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Q17938

- DAF36_CAEEL

UniProt

Q17938 - DAF36_CAEEL

Protein

Cholesterol desaturase daf-36

Gene

daf-36

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the production of 7-dehydrocholesterol (7-DHC) by the desaturation of the C7-C8 single bond of cholesterol. This reaction is the first step in the synthesis of the steroid hormone delta(7)-dafachronic acid. Dafachronic acids bind directly to the nuclear hormone receptor (NHR) daf-12, suppressing dauer formation and inducing reproductive growth.3 Publications

    Catalytic activityi

    Cholesterol + NADP+ = cholesta-5,7-dien-3-beta-ol + NADPH.2 Publications

    Cofactori

    Binds 1 2Fe-2S cluster per subunit.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi122 – 1221Iron-sulfur (2Fe-2S)Curated
    Metal bindingi124 – 1241Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation
    Metal bindingi143 – 1431Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
    Metal bindingi146 – 1461Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 7-dehydrocholesterol reductase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cholesterol metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-18486.
    UniPathwayiUPA01020.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cholesterol desaturase daf-36 (EC:1.3.1.21)
    Gene namesi
    Name:daf-36
    ORF Names:C12D8.5
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome V

    Organism-specific databases

    WormBaseiC12D8.5; CE34156; WBGene00007536; daf-36.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: WormBase
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi122 – 1221C → A: Abrogates cholesterol dehydrogenase activity. 1 Publication
    Mutagenesisi234 – 2341D → A: Abrogates cholesterol dehydrogenase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 428428Cholesterol desaturase daf-36PRO_0000421680Add
    BLAST

    Proteomic databases

    PaxDbiQ17938.
    PRIDEiQ17938.

    Expressioni

    Tissue specificityi

    Expressed in intestine at all postembryonic stages, including dauer. Expression is reduced in daf-2 mutants.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi6239.C12D8.5.

    Structurei

    3D structure databases

    ProteinModelPortaliQ17938.
    SMRiQ17938. Positions 79-244.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei6 – 2621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini81 – 187107RieskePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Rieske domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG4638.
    GeneTreeiENSGT00390000016856.
    HOGENOMiHOG000018897.
    InParanoidiQ17938.
    KOiK14938.
    OMAiPNGWYRV.
    OrthoDBiEOG7N0C5Q.
    PhylomeDBiQ17938.

    Family and domain databases

    Gene3Di2.102.10.10. 1 hit.
    InterProiIPR017941. Rieske_2Fe-2S.
    [Graphical view]
    PfamiPF00355. Rieske. 1 hit.
    [Graphical view]
    SUPFAMiSSF50022. SSF50022. 1 hit.
    PROSITEiPS51296. RIESKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q17938-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLEQIWGFL TAHPISVVTT ILIVYLIHIT LKPLNRVRRL GDVGLFFGKP    50
    ELKGFYRERQ LERLKLLRRV GDMPPVFPNG WYCVCESEKL ANNQIMEITV 100
    LGQFLSLIRS ESGAVYITDS YCPHIGANFN IGGRVVRDNC IQCPFHGWIF 150
    SAETGKCVEV PYDEGRIPEQ AKVTTWPCIE RNNNIYLWYH CDGAEPEWEI 200
    PEITEITDGF WHLGGRTEHE VMCHIQEIPE NGADIAHLNY LHKSAPPVTK 250
    GSDIIKTDLS DPQPAVQHVW DGKWEVKSEE DRHCGVMHLN QFMTFWGYKV 300
    PLTSSKLVAE QHGPGIVHML FDFGIWGKGV VFQTVTPEEA LLQRVRFRIF 350
    SNIPWFFVKF FMTVEAMQFE RDVFIWSNKK YIKSPLLVKN DGPIQKHRRW 400
    FSQFYTENSP KMLKDGSLSN QAKSIFDW 428
    Length:428
    Mass (Da):49,550
    Last modified:October 1, 2003 - v2
    Checksum:i93F0293DF344CBBA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73969 Genomic DNA. Translation: CAA98235.2.
    PIRiT19219.
    RefSeqiNP_505629.2. NM_073228.4.
    UniGeneiCel.4822.

    Genome annotation databases

    EnsemblMetazoaiC12D8.5; C12D8.5; WBGene00007536.
    GeneIDi179422.
    KEGGicel:CELE_C12D8.5.
    UCSCiC12D8.5. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73969 Genomic DNA. Translation: CAA98235.2 .
    PIRi T19219.
    RefSeqi NP_505629.2. NM_073228.4.
    UniGenei Cel.4822.

    3D structure databases

    ProteinModelPortali Q17938.
    SMRi Q17938. Positions 79-244.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6239.C12D8.5.

    Proteomic databases

    PaxDbi Q17938.
    PRIDEi Q17938.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai C12D8.5 ; C12D8.5 ; WBGene00007536 .
    GeneIDi 179422.
    KEGGi cel:CELE_C12D8.5.
    UCSCi C12D8.5. c. elegans.

    Organism-specific databases

    CTDi 179422.
    WormBasei C12D8.5 ; CE34156 ; WBGene00007536 ; daf-36.

    Phylogenomic databases

    eggNOGi COG4638.
    GeneTreei ENSGT00390000016856.
    HOGENOMi HOG000018897.
    InParanoidi Q17938.
    KOi K14938.
    OMAi PNGWYRV.
    OrthoDBi EOG7N0C5Q.
    PhylomeDBi Q17938.

    Enzyme and pathway databases

    UniPathwayi UPA01020 .
    BioCyci MetaCyc:MONOMER-18486.

    Miscellaneous databases

    NextBioi 905318.

    Family and domain databases

    Gene3Di 2.102.10.10. 1 hit.
    InterProi IPR017941. Rieske_2Fe-2S.
    [Graphical view ]
    Pfami PF00355. Rieske. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50022. SSF50022. 1 hit.
    PROSITEi PS51296. RIESKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    2. "Hormonal control of C. elegans dauer formation and life span by a Rieske-like oxygenase."
      Rottiers V., Motola D.L., Gerisch B., Cummins C.L., Nishiwaki K., Mangelsdorf D.J., Antebi A.
      Dev. Cell 10:473-482(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    3. "The Rieske oxygenase DAF-36 functions as a cholesterol 7-desaturase in steroidogenic pathways governing longevity."
      Wollam J., Magomedova L., Magner D.B., Shen Y., Rottiers V., Motola D.L., Mangelsdorf D.J., Cummins C.L., Antebi A.
      Aging Cell 10:879-884(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY.
    4. Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY, MUTAGENESIS OF CYS-122 AND ASP-234.

    Entry informationi

    Entry nameiDAF36_CAEEL
    AccessioniPrimary (citable) accession number: Q17938
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 6, 2013
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3