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Q17938

- DAF36_CAEEL

UniProt

Q17938 - DAF36_CAEEL

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Protein

Cholesterol desaturase daf-36

Gene

daf-36

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the production of 7-dehydrocholesterol (7-DHC) by the desaturation of the C7-C8 single bond of cholesterol. This reaction is the first step in the synthesis of the steroid hormone delta(7)-dafachronic acid. Dafachronic acids bind directly to the nuclear hormone receptor (NHR) daf-12, suppressing dauer formation and inducing reproductive growth.3 Publications

Catalytic activityi

Cholesterol + NADP+ = cholesta-5,7-dien-3-beta-ol + NADPH.2 Publications

Cofactori

Binds 1 2Fe-2S cluster per subunit.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi122 – 1221Iron-sulfur (2Fe-2S)Curated
Metal bindingi124 – 1241Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation
Metal bindingi143 – 1431Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi146 – 1461Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 7-dehydrocholesterol reductase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cholesterol metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18486.
UniPathwayiUPA01020.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol desaturase daf-36 (EC:1.3.1.21)
Gene namesi
Name:daf-36
ORF Names:C12D8.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome V

Organism-specific databases

WormBaseiC12D8.5; CE34156; WBGene00007536; daf-36.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: WormBase
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi122 – 1221C → A: Abrogates cholesterol dehydrogenase activity. 1 Publication
Mutagenesisi234 – 2341D → A: Abrogates cholesterol dehydrogenase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Cholesterol desaturase daf-36PRO_0000421680Add
BLAST

Proteomic databases

PaxDbiQ17938.
PRIDEiQ17938.

Expressioni

Tissue specificityi

Expressed in intestine at all postembryonic stages, including dauer. Expression is reduced in daf-2 mutants.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi6239.C12D8.5.

Structurei

3D structure databases

ProteinModelPortaliQ17938.
SMRiQ17938. Positions 79-244.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 187107RieskePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4638.
GeneTreeiENSGT00390000016856.
HOGENOMiHOG000018897.
InParanoidiQ17938.
KOiK14938.
OMAiPNGWYRV.
OrthoDBiEOG7N0C5Q.
PhylomeDBiQ17938.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
[Graphical view]
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q17938-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLEQIWGFL TAHPISVVTT ILIVYLIHIT LKPLNRVRRL GDVGLFFGKP
60 70 80 90 100
ELKGFYRERQ LERLKLLRRV GDMPPVFPNG WYCVCESEKL ANNQIMEITV
110 120 130 140 150
LGQFLSLIRS ESGAVYITDS YCPHIGANFN IGGRVVRDNC IQCPFHGWIF
160 170 180 190 200
SAETGKCVEV PYDEGRIPEQ AKVTTWPCIE RNNNIYLWYH CDGAEPEWEI
210 220 230 240 250
PEITEITDGF WHLGGRTEHE VMCHIQEIPE NGADIAHLNY LHKSAPPVTK
260 270 280 290 300
GSDIIKTDLS DPQPAVQHVW DGKWEVKSEE DRHCGVMHLN QFMTFWGYKV
310 320 330 340 350
PLTSSKLVAE QHGPGIVHML FDFGIWGKGV VFQTVTPEEA LLQRVRFRIF
360 370 380 390 400
SNIPWFFVKF FMTVEAMQFE RDVFIWSNKK YIKSPLLVKN DGPIQKHRRW
410 420
FSQFYTENSP KMLKDGSLSN QAKSIFDW
Length:428
Mass (Da):49,550
Last modified:October 1, 2003 - v2
Checksum:i93F0293DF344CBBA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z73969 Genomic DNA. Translation: CAA98235.2.
PIRiT19219.
RefSeqiNP_505629.2. NM_073228.4.
UniGeneiCel.4822.

Genome annotation databases

EnsemblMetazoaiC12D8.5; C12D8.5; WBGene00007536.
GeneIDi179422.
KEGGicel:CELE_C12D8.5.
UCSCiC12D8.5. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z73969 Genomic DNA. Translation: CAA98235.2 .
PIRi T19219.
RefSeqi NP_505629.2. NM_073228.4.
UniGenei Cel.4822.

3D structure databases

ProteinModelPortali Q17938.
SMRi Q17938. Positions 79-244.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 6239.C12D8.5.

Proteomic databases

PaxDbi Q17938.
PRIDEi Q17938.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai C12D8.5 ; C12D8.5 ; WBGene00007536 .
GeneIDi 179422.
KEGGi cel:CELE_C12D8.5.
UCSCi C12D8.5. c. elegans.

Organism-specific databases

CTDi 179422.
WormBasei C12D8.5 ; CE34156 ; WBGene00007536 ; daf-36.

Phylogenomic databases

eggNOGi COG4638.
GeneTreei ENSGT00390000016856.
HOGENOMi HOG000018897.
InParanoidi Q17938.
KOi K14938.
OMAi PNGWYRV.
OrthoDBi EOG7N0C5Q.
PhylomeDBi Q17938.

Enzyme and pathway databases

UniPathwayi UPA01020 .
BioCyci MetaCyc:MONOMER-18486.

Miscellaneous databases

NextBioi 905318.

Family and domain databases

Gene3Di 2.102.10.10. 1 hit.
InterProi IPR017941. Rieske_2Fe-2S.
[Graphical view ]
Pfami PF00355. Rieske. 1 hit.
[Graphical view ]
SUPFAMi SSF50022. SSF50022. 1 hit.
PROSITEi PS51296. RIESKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Hormonal control of C. elegans dauer formation and life span by a Rieske-like oxygenase."
    Rottiers V., Motola D.L., Gerisch B., Cummins C.L., Nishiwaki K., Mangelsdorf D.J., Antebi A.
    Dev. Cell 10:473-482(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  3. "The Rieske oxygenase DAF-36 functions as a cholesterol 7-desaturase in steroidogenic pathways governing longevity."
    Wollam J., Magomedova L., Magner D.B., Shen Y., Rottiers V., Motola D.L., Mangelsdorf D.J., Cummins C.L., Antebi A.
    Aging Cell 10:879-884(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY.
  4. Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY, MUTAGENESIS OF CYS-122 AND ASP-234.

Entry informationi

Entry nameiDAF36_CAEEL
AccessioniPrimary (citable) accession number: Q17938
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: October 1, 2003
Last modified: October 1, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3