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Protein

Cholesterol 7-desaturase

Gene

daf-36

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the production of 7-dehydrocholesterol (7-DHC) by the desaturation of the C7-C8 single bond of cholesterol. This reaction is the first step in the synthesis of the steroid hormone Delta7-dafachronic acid. Dafachronic acids bind directly to the nuclear hormone receptor (NHR) daf-12, suppressing dauer formation and inducing reproductive growth.3 Publications

Catalytic activityi

Cholesterol + O2 + NAD(P)H = cholesta-5,7-dien-3-beta-ol + NAD(P)+ + 2 H2O.2 Publications

Cofactori

[2Fe-2S] clusterPROSITE-ProRule annotationNote: Binds 1 [2Fe-2S] cluster per subunit.PROSITE-ProRule annotation

Pathwayi: dafachronic acid biosynthesis

This protein is involved in the pathway dafachronic acid biosynthesis, which is part of Steroid hormone biosynthesis.2 Publications
View all proteins of this organism that are known to be involved in the pathway dafachronic acid biosynthesis and in Steroid hormone biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi122Iron-sulfur (2Fe-2S)Curated1
Metal bindingi124Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1
Metal bindingi143Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi146Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18486
BRENDAi1.3.1.21 1045
UniPathwayiUPA01020

Chemistry databases

SwissLipidsiSLP:000000036
SLP:000000191

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol 7-desaturase1 Publication (EC:1.14.19.212 Publications)
Alternative name(s):
Cholesterol desaturase daf-36
Gene namesi
Name:daf-36
ORF Names:C12D8.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiC12D8.5 ; CE34156 ; WBGene00007536 ; daf-36

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei6 – 26HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi122C → A: Abrogates cholesterol dehydrogenase activity. 1 Publication1
Mutagenesisi234D → A: Abrogates cholesterol dehydrogenase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004216801 – 428Cholesterol 7-desaturaseAdd BLAST428

Proteomic databases

EPDiQ17938
PaxDbiQ17938
PeptideAtlasiQ17938

Expressioni

Tissue specificityi

Expressed in intestine at all postembryonic stages, including dauer. Expression is reduced in daf-2 mutants.1 Publication

Gene expression databases

BgeeiWBGene00007536

Interactioni

Protein-protein interaction databases

STRINGi6239.C12D8.5

Structurei

3D structure databases

ProteinModelPortaliQ17938
SMRiQ17938
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 187RieskePROSITE-ProRule annotationAdd BLAST107

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IDZ7 Eukaryota
COG4638 LUCA
GeneTreeiENSGT00390000016856
HOGENOMiHOG000018897
InParanoidiQ17938
KOiK14938
OMAiRTEHFIN
OrthoDBiEOG091G0ACN
PhylomeDBiQ17938

Family and domain databases

Gene3Di2.102.10.10, 1 hit
InterProiView protein in InterPro
IPR017941 Rieske_2Fe-2S
IPR036922 Rieske_2Fe-2S_sf
PfamiView protein in Pfam
PF00355 Rieske, 1 hit
SUPFAMiSSF50022 SSF50022, 1 hit
PROSITEiView protein in PROSITE
PS51296 RIESKE, 1 hit

Sequencei

Sequence statusi: Complete.

Q17938-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLEQIWGFL TAHPISVVTT ILIVYLIHIT LKPLNRVRRL GDVGLFFGKP
60 70 80 90 100
ELKGFYRERQ LERLKLLRRV GDMPPVFPNG WYCVCESEKL ANNQIMEITV
110 120 130 140 150
LGQFLSLIRS ESGAVYITDS YCPHIGANFN IGGRVVRDNC IQCPFHGWIF
160 170 180 190 200
SAETGKCVEV PYDEGRIPEQ AKVTTWPCIE RNNNIYLWYH CDGAEPEWEI
210 220 230 240 250
PEITEITDGF WHLGGRTEHE VMCHIQEIPE NGADIAHLNY LHKSAPPVTK
260 270 280 290 300
GSDIIKTDLS DPQPAVQHVW DGKWEVKSEE DRHCGVMHLN QFMTFWGYKV
310 320 330 340 350
PLTSSKLVAE QHGPGIVHML FDFGIWGKGV VFQTVTPEEA LLQRVRFRIF
360 370 380 390 400
SNIPWFFVKF FMTVEAMQFE RDVFIWSNKK YIKSPLLVKN DGPIQKHRRW
410 420
FSQFYTENSP KMLKDGSLSN QAKSIFDW
Length:428
Mass (Da):49,550
Last modified:October 1, 2003 - v2
Checksum:i93F0293DF344CBBA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73969 Genomic DNA Translation: CAA98235.2
PIRiT19219
RefSeqiNP_505629.2, NM_073228.4
UniGeneiCel.4822

Genome annotation databases

EnsemblMetazoaiC12D8.5; C12D8.5; WBGene00007536
GeneIDi179422
KEGGicel:CELE_C12D8.5
UCSCiC12D8.5 c. elegans

Similar proteinsi

Entry informationi

Entry nameiDAF36_CAEEL
AccessioniPrimary (citable) accession number: Q17938
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: October 1, 2003
Last modified: March 28, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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