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Protein

Cholesterol desaturase daf-36

Gene

daf-36

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the production of 7-dehydrocholesterol (7-DHC) by the desaturation of the C7-C8 single bond of cholesterol. This reaction is the first step in the synthesis of the steroid hormone delta(7)-dafachronic acid. Dafachronic acids bind directly to the nuclear hormone receptor (NHR) daf-12, suppressing dauer formation and inducing reproductive growth.3 Publications

Catalytic activityi

Cholesterol + NADP+ = cholesta-5,7-dien-3-beta-ol + NADPH.2 Publications

Cofactori

[2Fe-2S] clusterPROSITE-ProRule annotationNote: Binds 1 [2Fe-2S] cluster per subunit.PROSITE-ProRule annotation

Pathway: dafachronic acid biosynthesis

This protein is involved in the pathway dafachronic acid biosynthesis, which is part of Steroid hormone biosynthesis.2 Publications
View all proteins of this organism that are known to be involved in the pathway dafachronic acid biosynthesis and in Steroid hormone biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi122 – 1221Iron-sulfur (2Fe-2S)Curated
Metal bindingi124 – 1241Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation
Metal bindingi143 – 1431Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi146 – 1461Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18486.
BRENDAi1.3.1.21. 1045.
UniPathwayiUPA01020.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol desaturase daf-36 (EC:1.3.1.21)
Gene namesi
Name:daf-36
ORF Names:C12D8.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiC12D8.5; CE34156; WBGene00007536; daf-36.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: WormBase
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi122 – 1221C → A: Abrogates cholesterol dehydrogenase activity. 1 Publication
Mutagenesisi234 – 2341D → A: Abrogates cholesterol dehydrogenase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Cholesterol desaturase daf-36PRO_0000421680Add
BLAST

Proteomic databases

PaxDbiQ17938.
PRIDEiQ17938.

Expressioni

Tissue specificityi

Expressed in intestine at all postembryonic stages, including dauer. Expression is reduced in daf-2 mutants.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi6239.C12D8.5.

Structurei

3D structure databases

ProteinModelPortaliQ17938.
SMRiQ17938. Positions 74-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 187107RieskePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4638.
GeneTreeiENSGT00390000016856.
HOGENOMiHOG000018897.
InParanoidiQ17938.
KOiK14938.
OMAiRAECIQF.
OrthoDBiEOG7N0C5Q.
PhylomeDBiQ17938.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
[Graphical view]
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q17938-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLEQIWGFL TAHPISVVTT ILIVYLIHIT LKPLNRVRRL GDVGLFFGKP
60 70 80 90 100
ELKGFYRERQ LERLKLLRRV GDMPPVFPNG WYCVCESEKL ANNQIMEITV
110 120 130 140 150
LGQFLSLIRS ESGAVYITDS YCPHIGANFN IGGRVVRDNC IQCPFHGWIF
160 170 180 190 200
SAETGKCVEV PYDEGRIPEQ AKVTTWPCIE RNNNIYLWYH CDGAEPEWEI
210 220 230 240 250
PEITEITDGF WHLGGRTEHE VMCHIQEIPE NGADIAHLNY LHKSAPPVTK
260 270 280 290 300
GSDIIKTDLS DPQPAVQHVW DGKWEVKSEE DRHCGVMHLN QFMTFWGYKV
310 320 330 340 350
PLTSSKLVAE QHGPGIVHML FDFGIWGKGV VFQTVTPEEA LLQRVRFRIF
360 370 380 390 400
SNIPWFFVKF FMTVEAMQFE RDVFIWSNKK YIKSPLLVKN DGPIQKHRRW
410 420
FSQFYTENSP KMLKDGSLSN QAKSIFDW
Length:428
Mass (Da):49,550
Last modified:October 1, 2003 - v2
Checksum:i93F0293DF344CBBA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73969 Genomic DNA. Translation: CAA98235.2.
PIRiT19219.
RefSeqiNP_505629.2. NM_073228.4.
UniGeneiCel.4822.

Genome annotation databases

EnsemblMetazoaiC12D8.5; C12D8.5; WBGene00007536.
GeneIDi179422.
KEGGicel:CELE_C12D8.5.
UCSCiC12D8.5. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73969 Genomic DNA. Translation: CAA98235.2.
PIRiT19219.
RefSeqiNP_505629.2. NM_073228.4.
UniGeneiCel.4822.

3D structure databases

ProteinModelPortaliQ17938.
SMRiQ17938. Positions 74-407.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C12D8.5.

Proteomic databases

PaxDbiQ17938.
PRIDEiQ17938.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC12D8.5; C12D8.5; WBGene00007536.
GeneIDi179422.
KEGGicel:CELE_C12D8.5.
UCSCiC12D8.5. c. elegans.

Organism-specific databases

CTDi179422.
WormBaseiC12D8.5; CE34156; WBGene00007536; daf-36.

Phylogenomic databases

eggNOGiCOG4638.
GeneTreeiENSGT00390000016856.
HOGENOMiHOG000018897.
InParanoidiQ17938.
KOiK14938.
OMAiRAECIQF.
OrthoDBiEOG7N0C5Q.
PhylomeDBiQ17938.

Enzyme and pathway databases

UniPathwayiUPA01020.
BioCyciMetaCyc:MONOMER-18486.
BRENDAi1.3.1.21. 1045.

Miscellaneous databases

NextBioi905318.
PROiQ17938.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
[Graphical view]
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Hormonal control of C. elegans dauer formation and life span by a Rieske-like oxygenase."
    Rottiers V., Motola D.L., Gerisch B., Cummins C.L., Nishiwaki K., Mangelsdorf D.J., Antebi A.
    Dev. Cell 10:473-482(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  3. "The Rieske oxygenase DAF-36 functions as a cholesterol 7-desaturase in steroidogenic pathways governing longevity."
    Wollam J., Magomedova L., Magner D.B., Shen Y., Rottiers V., Motola D.L., Mangelsdorf D.J., Cummins C.L., Antebi A.
    Aging Cell 10:879-884(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY.
  4. Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY, MUTAGENESIS OF CYS-122 AND ASP-234.

Entry informationi

Entry nameiDAF36_CAEEL
AccessioniPrimary (citable) accession number: Q17938
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: October 1, 2003
Last modified: April 29, 2015
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.