Reviewed, UniProtKB/Swiss-Prot Q17820 (SAE1_CAEEL)

Names and origin

Protein names

Recommended name:
    SUMO-activating enzyme subunit aos-1

Gene names

Name:	aos-1
ORF Names:	C08B6.9

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The dimeric enzyme acts as a E1 ligase for smo-1. It mediates ATP-dependent activation of smo-1 and formation of a thioester with a conserved cysteine residue on uba-2 Probable.
Pathway	Protein modification; protein sumoylation.
Subunit structure	Heterodimer of aos-1 and uba-2.
Sequence similarities	Belongs to the ubiquitin-activating E1 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW nematode larval development Inferred from mutant phenotype. Source: WormBase oogenesis Inferred from mutant phenotype. Source: WormBase positive regulation of embryonic development Inferred from mutant phenotype. Source: WormBase
Molecular function	binding Inferred from electronic annotation. Source: InterPro ligase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	SUMO-activating enzyme subunit aos-1		PRO_0000270189

Sequences

	Sequence		Length	Mass (Da)
	Q17820-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2897C0F918E881B6	FASTA	343	38,738
	10 20 30 40 50 60 MEVSKAEQAI YDRQIRLWGM EAQNKIRNSK VLIIGGKQLG AEVAKTLSLA GVDEMHLVDH 70 80 90 100 110 120 RLVDTEEIGM NFLYDASVDN SKMTKWAASY NFLYNLNRNV KLFIVEEDVL SKNDSEIEEY 130 140 150 160 170 180 LTKFTLVVVL DESYERTAKV NNICRKHHIR FISGAIYGWI GYAFFDFDGH AYLVKAKSPD 190 200 210 220 230 240 CLNEEESETG KTSTVVTVDE EFVLETFSYP SFVETLNSDF TAKKIVRKCK RIVPTSYFLV 250 260 270 280 290 300 KSMLRASSEN KLTGVTENDI EKLIPIWNEE VAAGNHTIDM QPVQPDRFDH LFGPNFGPTA 310 320 330 340 ACVGGVIGQE AIKSISEGKN PLRNLFIYTG FESTGFMCNF PPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Functional and phylogenetic analysis of the ubiquitylation system in Caenorhabditis elegans: ubiquitin-conjugating enzymes, ubiquitin-activating enzymes, and ubiquitin-like proteins." Jones D., Crowe E., Stevens T.A., Candido E.P.M. Genome Biol. 3:RESEARCH0002.1-RESEARCH0002.15(2002) [PubMed: 11806825] [Abstract] Cited for: FUNCTION.
[3]	"SUMO modification is required for in vivo Hox gene regulation by the Caenorhabditis elegans Polycomb group protein SOP-2." Zhang H., Smolen G.A., Palmer R., Christoforou A., van den Heuvel S., Haber D.A. Nat. Genet. 36:507-511(2004) [PubMed: 15107848] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z72502 Genomic DNA. Translation: CAA96591.1.
PIR	T19082.
RefSeq	NP_505604.1.
UniGene	Cel.30581
3D structure databases
HSSP	HSSP built from PDB template 1Y8Q based on UniProtKB Q9UBE0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q17820. 1 interaction.
STRING	Q17820.
Genome annotation databases
Ensembl	C08B6.9; C08B6.9; C08B6.9; Caenorhabditis elegans. [Genome view]
GeneID	179409.
KEGG	cel:C08B6.9.
NMPDR	fig|6239.3.peg.19316.
UCSC	C08B6.9. c. elegans.
Organism-specific databases
CTD	179409.
WormBase	WBGene00000142. aos-1.
WormPep	C08B6.9. CE05246. [WorfDB]
Phylogenomic databases
OMA	IALYDRQ
Gene expression databases
ArrayExpress	Q17820.
Family and domain databases
InterPro	IPR009036. Molybdenum_cofac_synth_MoeB. IPR016040. NAD(P)-bd_dom. IPR000594. ThiF_NAD_FAD_bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00899. ThiF. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	905268.

Entry information

Entry name

SAE1_CAEEL

Accession

Primary (citable) accession number: Q17820

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project