Reviewed, UniProtKB/Swiss-Prot Q17693 (MTHR_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable methylenetetrahydrofolate reductase
    EC=1.5.1.20

Gene names

ORF Names:

C06A8.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	663 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
mdf-2	Q9NGT3	1	EBI-313786,EBI-328699

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 663	663	Probable methylenetetrahydrofolate reductase		PRO_0000190248

Sequences

	Sequence		Length	Mass (Da)
	Q17693-1 [UniParc]. Last modified June 20, 2002. Version 2. Checksum: EA94806A2C3BC1CD	FASTA	663	75,487
	10 20 30 40 50 60 MTNTGETKVI ESHGTIKKID SLSTMPYCGV ETDENAVVEE KITLESGKSW SPKHYELLHE 70 80 90 100 110 120 RIERLIDEKQ QFFSLEFFPP RFVNGVPNFL ERVERLSEGG SVFVDMTWHM GSDPANVDKV 130 140 150 160 170 180 TSSSSIAASM LDYCGVDTML HMTCVQYNKA DTLKHLEQAK AMGLRSILAL RGDLPPGTEL 190 200 210 220 230 240 EDTHQFRALD MIRWIREEYG NYFSIGCAGY PLGHPQAPSY KADLMYLKAK CDAGANFVIT 250 260 270 280 290 300 QLFFEAETFE KFVRDCREIG ITQPIIPGIM PIMGYESIKR IAKLSQLEIP QHILDDLEPI 310 320 330 340 350 360 KHDDDAVQKY GTERCIEMCR RLLDNGTAPS IHLYTMNREG SIREILKSLG LWKLEGDRVF 370 380 390 400 410 420 PWKNRSQHPI RCLESVRPIY WSFRPRSYIT RTRDWDQFPN GRWGNSSSPA FGDVSSYYLS 430 440 450 460 470 480 NLTTVRNADD RLAMFGANIE SFEDVKRVFI NYITQAPNAD GVKVTVLPWT EAETGVQPET 490 500 510 520 530 540 SLISEQLVWC NENGILTVNS QPSVNGAPST DPLVGWGKPG GYCYQKAYLE CFMTAELSDK 550 560 570 580 590 600 LIQIIEREFP VRVNYHAINK DSTFDKTNSE ETTPIAVTWG VFPGSEIAQP TVVDPLSFRA 610 620 630 640 650 660 WRDEAYQMWM AQWGDFYPKE SKSYGVIKAV HDEFRLVTLV DNDFQKPSVL FDVLQKALDE LKK

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U39849 Genomic DNA. Translation: AAA81048.2.
PIR	T15423.
RefSeq	NP_741027.1.
UniGene	Cel.17243
3D structure databases
SMR	Q17693. Positions 62-353.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-26125N.
IntAct	Q17693. 3 interactions.
STRING	Q17693.
Proteomic databases
PRIDE	Q17693.
Genome annotation databases
Ensembl	C06A8.1a.1; C06A8.1a.1; C06A8.1; Caenorhabditis elegans. [Genome view] C06A8.1a.2; C06A8.1a.2; C06A8.1; Caenorhabditis elegans. [Genome view]
GeneID	174254.
KEGG	cel:C06A8.1.
UCSC	C06A8.1a.1. c. elegans.
Organism-specific databases
CTD	174254.
WormBase	WBGene00015512. C06A8.1.
WormPep	C06A8.1. CE30593. [WorfDB]
Phylogenomic databases
eggNOG	meNOG04108.
HOGENOM	HBG649727.
InParanoid	Q17693.
OMA	DITWHPA.
PhylomeDB	Q17693.
Enzyme and pathway databases
BRENDA	1.5.1.20. 672.
Gene expression databases
ArrayExpress	Q17693.
Family and domain databases
InterPro	IPR004621. Fadh2_euk. IPR003171. Mehydrof_redctse. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00677. fadh2_euk. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	883243.

Entry information

Entry name

MTHR_CAEEL

Accession

Primary (citable) accession number: Q17693

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	June 20, 2002
Last modified:	February 9, 2010
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project