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Protein

Probable methylenetetrahydrofolate reductase

Gene

C06A8.1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei76 – 761Proton donor/acceptorBy similarity
Binding sitei141 – 1411FADBy similarity
Binding sitei173 – 1731SubstrateBy similarity
Binding sitei210 – 2101FADBy similarity
Binding sitei223 – 2231FADBy similarity
Binding sitei230 – 2301FADBy similarity
Binding sitei241 – 2411SubstrateBy similarity
Binding sitei334 – 3341SubstrateBy similarity
Binding sitei338 – 3381SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi76 – 816NADBy similarity
Nucleotide bindingi107 – 1082NAD and FADBy similarity
Nucleotide bindingi171 – 1733FADBy similarity
Nucleotide bindingi214 – 2174FADBy similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: GO_Central

GO - Biological processi

  1. methionine metabolic process Source: InterPro
  2. one-carbon metabolic process Source: GO_Central
  3. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BRENDAi1.5.1.20. 1045.
ReactomeiREACT_306849. Metabolism of folate and pterines.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
ORF Names:C06A8.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiC06A8.1a; CE30593; WBGene00015512.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 663663Probable methylenetetrahydrofolate reductasePRO_0000190248Add
BLAST

Proteomic databases

PaxDbiQ17693.
PRIDEiQ17693.

Expressioni

Gene expression databases

ExpressionAtlasiQ17693. baseline and differential.

Interactioni

Protein-protein interaction databases

BioGridi39587. 3 interactions.
DIPiDIP-26125N.
IntActiQ17693. 3 interactions.
MINTiMINT-212490.
STRINGi6239.C06A8.1a.2.

Structurei

3D structure databases

ProteinModelPortaliQ17693.
SMRiQ17693. Positions 72-351.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
GeneTreeiENSGT00390000012490.
HOGENOMiHOG000246234.
InParanoidiQ17693.
KOiK00297.
OMAiYLEFFVS.
PhylomeDBiQ17693.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q17693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNTGETKVI ESHGTIKKID SLSTMPYCGV ETDENAVVEE KITLESGKSW
60 70 80 90 100
SPKHYELLHE RIERLIDEKQ QFFSLEFFPP RFVNGVPNFL ERVERLSEGG
110 120 130 140 150
SVFVDMTWHM GSDPANVDKV TSSSSIAASM LDYCGVDTML HMTCVQYNKA
160 170 180 190 200
DTLKHLEQAK AMGLRSILAL RGDLPPGTEL EDTHQFRALD MIRWIREEYG
210 220 230 240 250
NYFSIGCAGY PLGHPQAPSY KADLMYLKAK CDAGANFVIT QLFFEAETFE
260 270 280 290 300
KFVRDCREIG ITQPIIPGIM PIMGYESIKR IAKLSQLEIP QHILDDLEPI
310 320 330 340 350
KHDDDAVQKY GTERCIEMCR RLLDNGTAPS IHLYTMNREG SIREILKSLG
360 370 380 390 400
LWKLEGDRVF PWKNRSQHPI RCLESVRPIY WSFRPRSYIT RTRDWDQFPN
410 420 430 440 450
GRWGNSSSPA FGDVSSYYLS NLTTVRNADD RLAMFGANIE SFEDVKRVFI
460 470 480 490 500
NYITQAPNAD GVKVTVLPWT EAETGVQPET SLISEQLVWC NENGILTVNS
510 520 530 540 550
QPSVNGAPST DPLVGWGKPG GYCYQKAYLE CFMTAELSDK LIQIIEREFP
560 570 580 590 600
VRVNYHAINK DSTFDKTNSE ETTPIAVTWG VFPGSEIAQP TVVDPLSFRA
610 620 630 640 650
WRDEAYQMWM AQWGDFYPKE SKSYGVIKAV HDEFRLVTLV DNDFQKPSVL
660
FDVLQKALDE LKK
Length:663
Mass (Da):75,487
Last modified:June 20, 2002 - v2
Checksum:iEA94806A2C3BC1CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080131 Genomic DNA. Translation: CCD61458.1.
PIRiT15423.
RefSeqiNP_741027.1. NM_171024.4.
UniGeneiCel.17243.

Genome annotation databases

EnsemblMetazoaiC06A8.1a; C06A8.1a; WBGene00015512.
GeneIDi174254.
KEGGicel:CELE_C06A8.1.
UCSCiC06A8.1a.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080131 Genomic DNA. Translation: CCD61458.1.
PIRiT15423.
RefSeqiNP_741027.1. NM_171024.4.
UniGeneiCel.17243.

3D structure databases

ProteinModelPortaliQ17693.
SMRiQ17693. Positions 72-351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi39587. 3 interactions.
DIPiDIP-26125N.
IntActiQ17693. 3 interactions.
MINTiMINT-212490.
STRINGi6239.C06A8.1a.2.

Proteomic databases

PaxDbiQ17693.
PRIDEiQ17693.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC06A8.1a; C06A8.1a; WBGene00015512.
GeneIDi174254.
KEGGicel:CELE_C06A8.1.
UCSCiC06A8.1a.1. c. elegans.

Organism-specific databases

CTDi174254.
WormBaseiC06A8.1a; CE30593; WBGene00015512.

Phylogenomic databases

eggNOGiCOG0685.
GeneTreeiENSGT00390000012490.
HOGENOMiHOG000246234.
InParanoidiQ17693.
KOiK00297.
OMAiYLEFFVS.
PhylomeDBiQ17693.

Enzyme and pathway databases

UniPathwayiUPA00193.
BRENDAi1.5.1.20. 1045.
ReactomeiREACT_306849. Metabolism of folate and pterines.

Miscellaneous databases

NextBioi883243.
PROiQ17693.

Gene expression databases

ExpressionAtlasiQ17693. baseline and differential.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiMTHR_CAEEL
AccessioniPrimary (citable) accession number: Q17693
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 20, 2002
Last modified: April 1, 2015
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.