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Protein

Probable methylenetetrahydrofolate reductase

Gene

mthf-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei76Proton donor/acceptorBy similarity1
Binding sitei141FADBy similarity1
Binding sitei173SubstrateBy similarity1
Binding sitei210FADBy similarity1
Binding sitei223FADBy similarity1
Binding sitei230FADBy similarity1
Binding sitei241SubstrateBy similarity1
Binding sitei334SubstrateBy similarity1
Binding sitei338SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi76 – 81NADBy similarity6
Nucleotide bindingi107 – 108NAD and FADBy similarity2
Nucleotide bindingi171 – 173FADBy similarity3
Nucleotide bindingi214 – 217FADBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

BRENDAi1.5.1.20 1045
ReactomeiR-CEL-196757 Metabolism of folate and pterines
UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
Probable methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:mthf-1Imported
ORF Names:C06A8.1Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiC06A8.1a ; CE30593 ; WBGene00015512 ; mthf-1
C06A8.1b ; CE31291 ; WBGene00015512 ; mthf-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902481 – 663Probable methylenetetrahydrofolate reductaseAdd BLAST663

Proteomic databases

EPDiQ17693
PaxDbiQ17693
PeptideAtlasiQ17693
PRIDEiQ17693

PTM databases

iPTMnetiQ17693

Expressioni

Gene expression databases

BgeeiWBGene00015512

Interactioni

Protein-protein interaction databases

BioGridi39587, 3 interactors
DIPiDIP-26125N
IntActiQ17693, 3 interactors
STRINGi6239.C06A8.1a.2

Structurei

3D structure databases

ProteinModelPortaliQ17693
SMRiQ17693
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0564 Eukaryota
COG0685 LUCA
GeneTreeiENSGT00390000012490
HOGENOMiHOG000246234
InParanoidiQ17693
KOiK00297
OMAiMESGDKW
OrthoDBiEOG091G0YQK
PhylomeDBiQ17693

Family and domain databases

CDDicd00537 MTHFR, 1 hit
InterProiView protein in InterPro
IPR029041 FAD-linked_oxidoreductase-like
IPR004621 Fadh2_euk
IPR003171 Mehydrof_redctse
PfamiView protein in Pfam
PF02219 MTHFR, 1 hit
SUPFAMiSSF51730 SSF51730, 1 hit
TIGRFAMsiTIGR00677 fadh2_euk, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform aImported (identifier: Q17693-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTNTGETKVI ESHGTIKKID SLSTMPYCGV ETDENAVVEE KITLESGKSW
60 70 80 90 100
SPKHYELLHE RIERLIDEKQ QFFSLEFFPP RFVNGVPNFL ERVERLSEGG
110 120 130 140 150
SVFVDMTWHM GSDPANVDKV TSSSSIAASM LDYCGVDTML HMTCVQYNKA
160 170 180 190 200
DTLKHLEQAK AMGLRSILAL RGDLPPGTEL EDTHQFRALD MIRWIREEYG
210 220 230 240 250
NYFSIGCAGY PLGHPQAPSY KADLMYLKAK CDAGANFVIT QLFFEAETFE
260 270 280 290 300
KFVRDCREIG ITQPIIPGIM PIMGYESIKR IAKLSQLEIP QHILDDLEPI
310 320 330 340 350
KHDDDAVQKY GTERCIEMCR RLLDNGTAPS IHLYTMNREG SIREILKSLG
360 370 380 390 400
LWKLEGDRVF PWKNRSQHPI RCLESVRPIY WSFRPRSYIT RTRDWDQFPN
410 420 430 440 450
GRWGNSSSPA FGDVSSYYLS NLTTVRNADD RLAMFGANIE SFEDVKRVFI
460 470 480 490 500
NYITQAPNAD GVKVTVLPWT EAETGVQPET SLISEQLVWC NENGILTVNS
510 520 530 540 550
QPSVNGAPST DPLVGWGKPG GYCYQKAYLE CFMTAELSDK LIQIIEREFP
560 570 580 590 600
VRVNYHAINK DSTFDKTNSE ETTPIAVTWG VFPGSEIAQP TVVDPLSFRA
610 620 630 640 650
WRDEAYQMWM AQWGDFYPKE SKSYGVIKAV HDEFRLVTLV DNDFQKPSVL
660
FDVLQKALDE LKK
Note: No experimental confirmation available.
Length:663
Mass (Da):75,487
Last modified:June 20, 2002 - v2
Checksum:iEA94806A2C3BC1CD
GO
Isoform bImported (identifier: Q17693-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: MTNTGETKVIESHGTIKKIDSLSTMPYC → MAVLSKN

Note: No experimental confirmation available.
Show »
Length:642
Mass (Da):73,163
Checksum:i2CB45319226E736A
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0576551 – 28MTNTG…TMPYC → MAVLSKN in isoform b. CuratedAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080131 Genomic DNA Translation: CCD61458.1
FO080131 Genomic DNA Translation: CCD61459.1
PIRiT15423
RefSeqiNP_741027.1, NM_171024.4 [Q17693-1]
NP_741028.1, NM_171025.6 [Q17693-2]
UniGeneiCel.17243

Genome annotation databases

EnsemblMetazoaiC06A8.1a.1; C06A8.1a.1; WBGene00015512 [Q17693-1]
C06A8.1a.2; C06A8.1a.2; WBGene00015512 [Q17693-1]
GeneIDi174254
KEGGicel:CELE_C06A8.1
UCSCiC06A8.1a.1 c. elegans [Q17693-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMTHR_CAEEL
AccessioniPrimary (citable) accession number: Q17693
Secondary accession number(s): G8JXY2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 20, 2002
Last modified: March 28, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health