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Protein

Probable methylenetetrahydrofolate reductase

Gene

mthf-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei76Proton donor/acceptorBy similarity1
Binding sitei141FADBy similarity1
Binding sitei173SubstrateBy similarity1
Binding sitei210FADBy similarity1
Binding sitei223FADBy similarity1
Binding sitei230FADBy similarity1
Binding sitei241SubstrateBy similarity1
Binding sitei334SubstrateBy similarity1
Binding sitei338SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi76 – 81NADBy similarity6
Nucleotide bindingi107 – 108NAD and FADBy similarity2
Nucleotide bindingi171 – 173FADBy similarity3
Nucleotide bindingi214 – 217FADBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BRENDAi1.5.1.20. 1045.
ReactomeiR-CEL-196757. Metabolism of folate and pterines.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:mthf-1Imported
ORF Names:C06A8.1Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiC06A8.1a; CE30593; WBGene00015512; mthf-1.
C06A8.1b; CE31291; WBGene00015512; mthf-1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902481 – 663Probable methylenetetrahydrofolate reductaseAdd BLAST663

Proteomic databases

EPDiQ17693.
PaxDbiQ17693.
PeptideAtlasiQ17693.
PRIDEiQ17693.

PTM databases

iPTMnetiQ17693.

Expressioni

Gene expression databases

BgeeiWBGene00015512.

Interactioni

Protein-protein interaction databases

BioGridi39587. 3 interactors.
DIPiDIP-26125N.
IntActiQ17693. 3 interactors.
MINTiMINT-212490.
STRINGi6239.C06A8.1a.2.

Structurei

3D structure databases

ProteinModelPortaliQ17693.
SMRiQ17693.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0564. Eukaryota.
COG0685. LUCA.
GeneTreeiENSGT00390000012490.
HOGENOMiHOG000246234.
InParanoidiQ17693.
KOiK00297.
OMAiVVGWGPS.
OrthoDBiEOG091G0YQK.
PhylomeDBiQ17693.

Family and domain databases

CDDicd00537. MTHFR. 1 hit.
Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform aImported (identifier: Q17693-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTNTGETKVI ESHGTIKKID SLSTMPYCGV ETDENAVVEE KITLESGKSW
60 70 80 90 100
SPKHYELLHE RIERLIDEKQ QFFSLEFFPP RFVNGVPNFL ERVERLSEGG
110 120 130 140 150
SVFVDMTWHM GSDPANVDKV TSSSSIAASM LDYCGVDTML HMTCVQYNKA
160 170 180 190 200
DTLKHLEQAK AMGLRSILAL RGDLPPGTEL EDTHQFRALD MIRWIREEYG
210 220 230 240 250
NYFSIGCAGY PLGHPQAPSY KADLMYLKAK CDAGANFVIT QLFFEAETFE
260 270 280 290 300
KFVRDCREIG ITQPIIPGIM PIMGYESIKR IAKLSQLEIP QHILDDLEPI
310 320 330 340 350
KHDDDAVQKY GTERCIEMCR RLLDNGTAPS IHLYTMNREG SIREILKSLG
360 370 380 390 400
LWKLEGDRVF PWKNRSQHPI RCLESVRPIY WSFRPRSYIT RTRDWDQFPN
410 420 430 440 450
GRWGNSSSPA FGDVSSYYLS NLTTVRNADD RLAMFGANIE SFEDVKRVFI
460 470 480 490 500
NYITQAPNAD GVKVTVLPWT EAETGVQPET SLISEQLVWC NENGILTVNS
510 520 530 540 550
QPSVNGAPST DPLVGWGKPG GYCYQKAYLE CFMTAELSDK LIQIIEREFP
560 570 580 590 600
VRVNYHAINK DSTFDKTNSE ETTPIAVTWG VFPGSEIAQP TVVDPLSFRA
610 620 630 640 650
WRDEAYQMWM AQWGDFYPKE SKSYGVIKAV HDEFRLVTLV DNDFQKPSVL
660
FDVLQKALDE LKK
Note: No experimental confirmation available.
Length:663
Mass (Da):75,487
Last modified:June 20, 2002 - v2
Checksum:iEA94806A2C3BC1CD
GO
Isoform bImported (identifier: Q17693-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: MTNTGETKVIESHGTIKKIDSLSTMPYC → MAVLSKN

Note: No experimental confirmation available.
Show »
Length:642
Mass (Da):73,163
Checksum:i2CB45319226E736A
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0576551 – 28MTNTG…TMPYC → MAVLSKN in isoform b. CuratedAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080131 Genomic DNA. Translation: CCD61458.1.
FO080131 Genomic DNA. Translation: CCD61459.1.
PIRiT15423.
RefSeqiNP_741027.1. NM_171024.4. [Q17693-1]
NP_741028.1. NM_171025.6. [Q17693-2]
UniGeneiCel.17243.

Genome annotation databases

EnsemblMetazoaiC06A8.1a; C06A8.1a; WBGene00015512. [Q17693-1]
GeneIDi174254.
KEGGicel:CELE_C06A8.1.
UCSCiC06A8.1a.1. c. elegans. [Q17693-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080131 Genomic DNA. Translation: CCD61458.1.
FO080131 Genomic DNA. Translation: CCD61459.1.
PIRiT15423.
RefSeqiNP_741027.1. NM_171024.4. [Q17693-1]
NP_741028.1. NM_171025.6. [Q17693-2]
UniGeneiCel.17243.

3D structure databases

ProteinModelPortaliQ17693.
SMRiQ17693.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi39587. 3 interactors.
DIPiDIP-26125N.
IntActiQ17693. 3 interactors.
MINTiMINT-212490.
STRINGi6239.C06A8.1a.2.

PTM databases

iPTMnetiQ17693.

Proteomic databases

EPDiQ17693.
PaxDbiQ17693.
PeptideAtlasiQ17693.
PRIDEiQ17693.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC06A8.1a; C06A8.1a; WBGene00015512. [Q17693-1]
GeneIDi174254.
KEGGicel:CELE_C06A8.1.
UCSCiC06A8.1a.1. c. elegans. [Q17693-1]

Organism-specific databases

CTDi174254.
WormBaseiC06A8.1a; CE30593; WBGene00015512; mthf-1.
C06A8.1b; CE31291; WBGene00015512; mthf-1.

Phylogenomic databases

eggNOGiKOG0564. Eukaryota.
COG0685. LUCA.
GeneTreeiENSGT00390000012490.
HOGENOMiHOG000246234.
InParanoidiQ17693.
KOiK00297.
OMAiVVGWGPS.
OrthoDBiEOG091G0YQK.
PhylomeDBiQ17693.

Enzyme and pathway databases

UniPathwayiUPA00193.
BRENDAi1.5.1.20. 1045.
ReactomeiR-CEL-196757. Metabolism of folate and pterines.

Miscellaneous databases

PROiQ17693.

Gene expression databases

BgeeiWBGene00015512.

Family and domain databases

CDDicd00537. MTHFR. 1 hit.
Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTHR_CAEEL
AccessioniPrimary (citable) accession number: Q17693
Secondary accession number(s): G8JXY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 20, 2002
Last modified: November 2, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.