Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative methionine synthase reductase

Gene

tag-165

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the reductive regeneration of cob(I)alamin cofactor required for the maintenance of methionine synthase in a functional state.By similarity

Catalytic activityi

2 [methionine synthase]-methylcob(I)alamin + 2 S-adenosylhomocysteine + NADP+ = 2 [methionine synthase]-cob(II)alamin + NADPH + 2 S-adenosyl-L-methionine.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarity
  • FMNBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei293 – 2931NADPBy similarity
Binding sitei643 – 6431NADPBy similarity
Binding sitei681 – 6811FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi93 – 12432FMNPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi455 – 4584FADBy similarity
Nucleotide bindingi488 – 4914FADBy similarity
Nucleotide bindingi607 – 6093NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-CEL-156581. Methylation.
R-CEL-1614635. Sulfur amino acid metabolism.
R-CEL-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative methionine synthase reductase (EC:1.16.1.8)
Short name:
MSR
Gene namesi
Name:tag-165
ORF Names:C01G6.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiC01G6.6a; CE00868; WBGene00006510; tag-165.
C01G6.6b; CE32562; WBGene00006510; tag-165.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 682682Putative methionine synthase reductasePRO_0000096638Add
BLAST

Proteomic databases

EPDiQ17574.
PaxDbiQ17574.
PRIDEiQ17574.

PTM databases

iPTMnetiQ17574.

Expressioni

Gene expression databases

BgeeiWBGene00006510.

Interactioni

Protein-protein interaction databases

STRINGi6239.C01G6.6a.2.

Structurei

3D structure databases

ProteinModelPortaliQ17574.
SMRiQ17574. Positions 3-682.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 147144Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini271 – 516246FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1158. Eukaryota.
COG0369. LUCA.
GeneTreeiENSGT00840000129757.
HOGENOMiHOG000007485.
InParanoidiQ17574.
KOiK00597.
OMAiFSVICPN.
OrthoDBiEOG091G045Q.
PhylomeDBiQ17574.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q17574-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDFLIAFGS QTGQAETIAK SLKEKAELIG LTPRLHALDE NEKKFNLNEE
60 70 80 90 100
KLCAIVVSST GDGDAPDNCA RFVRRINRNS LENEYLKNLD YVLLGLGDSN
110 120 130 140 150
YSSYQTIPRK IDKQLTALGA NRLFDRAEAD DQVGLELEVE PWIEKFFATL
160 170 180 190 200
ASRFDISADK MNAITESSNL KLNQVKTEEE KKALLQKRIE DEESDDEGRG
210 220 230 240 250
RVIGIDMLIP EHYDYPEISL LKGSQTLSND ENLRVPIAPQ PFIVSSVSNR
260 270 280 290 300
KLPEDTKLEW QNLCKMPGVV TKPFEVLVVS AEFVTDPFSK KIKTKRMITV
310 320 330 340 350
DFGDHAAELQ YEPGDAIYFC VPNPALEVNF ILKRCGVLDI ADQQCELSIN
360 370 380 390 400
PKTEKINAQI PGHVHKITTL RHMFTTCLDI RRAPGRPLIR VLAESTSDPN
410 420 430 440 450
EKRRLLELCS AQGMKDFTDF VRTPGLSLAD MLFAFPNVKP PVDRLIELLP
460 470 480 490 500
RLIPRPYSMS SYENRKARLI YSEMEFPATD GRRHSRKGLA TDWLNSLRIG
510 520 530 540 550
DKVQVLGKEP ARFRLPPLGM TKNSAGKLPL LMVGPGTGVS VFLSFLHFLR
560 570 580 590 600
KLKQDSPSDF VDVPRVLFFG CRDSSVDAIY MSELEMFVSE GILTDLIICE
610 620 630 640 650
SEQKGERVQD GLRKYLDKVL PFLTASTESK IFICGDAKGM SKDVWQCFSD
660 670 680
IVASDQGIPD LEAKKKLMDL KKSDQYIEDV WG
Note: No experimental confirmation available.
Length:682
Mass (Da):76,834
Last modified:November 1, 1996 - v1
Checksum:iC7E6D9AA07C1D2FD
GO
Isoform b (identifier: Q17574-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     133-133: V → T
     134-682: Missing.

Note: No experimental confirmation available.
Show »
Length:133
Mass (Da):14,842
Checksum:i86824BE4605CE43E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei133 – 1331V → T in isoform b. CuratedVSP_020538
Alternative sequencei134 – 682549Missing in isoform b. CuratedVSP_020539Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35595 Genomic DNA. Translation: CAA84637.1.
Z35595 Genomic DNA. Translation: CAD57684.1.
PIRiT18838.
RefSeqiNP_495978.1. NM_063577.4. [Q17574-1]
UniGeneiCel.16006.

Genome annotation databases

EnsemblMetazoaiC01G6.6a.1; C01G6.6a.1; WBGene00006510. [Q17574-1]
C01G6.6a.2; C01G6.6a.2; WBGene00006510. [Q17574-1]
GeneIDi174471.
KEGGicel:CELE_C01G6.6.
UCSCiC01G6.6b.1. c. elegans. [Q17574-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35595 Genomic DNA. Translation: CAA84637.1.
Z35595 Genomic DNA. Translation: CAD57684.1.
PIRiT18838.
RefSeqiNP_495978.1. NM_063577.4. [Q17574-1]
UniGeneiCel.16006.

3D structure databases

ProteinModelPortaliQ17574.
SMRiQ17574. Positions 3-682.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C01G6.6a.2.

PTM databases

iPTMnetiQ17574.

Proteomic databases

EPDiQ17574.
PaxDbiQ17574.
PRIDEiQ17574.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC01G6.6a.1; C01G6.6a.1; WBGene00006510. [Q17574-1]
C01G6.6a.2; C01G6.6a.2; WBGene00006510. [Q17574-1]
GeneIDi174471.
KEGGicel:CELE_C01G6.6.
UCSCiC01G6.6b.1. c. elegans. [Q17574-1]

Organism-specific databases

CTDi174471.
WormBaseiC01G6.6a; CE00868; WBGene00006510; tag-165.
C01G6.6b; CE32562; WBGene00006510; tag-165.

Phylogenomic databases

eggNOGiKOG1158. Eukaryota.
COG0369. LUCA.
GeneTreeiENSGT00840000129757.
HOGENOMiHOG000007485.
InParanoidiQ17574.
KOiK00597.
OMAiFSVICPN.
OrthoDBiEOG091G045Q.
PhylomeDBiQ17574.

Enzyme and pathway databases

ReactomeiR-CEL-156581. Methylation.
R-CEL-1614635. Sulfur amino acid metabolism.
R-CEL-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Miscellaneous databases

PROiQ17574.

Gene expression databases

BgeeiWBGene00006510.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTRR_CAEEL
AccessioniPrimary (citable) accession number: Q17574
Secondary accession number(s): Q8I4N2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.