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Protein

Fatty acid amide hydrolase 1

Gene

faah-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Degrades bioactive fatty acid amides like palmitoyl ethanolamide (PEA), palmitoleoyl ethanolamide (POEA), oleoyl ethanolamide (OEA), linoleoyl ethanolamide (LOEA), arachidonoyl ethanolamide (anandamide, or AEA), and eicosapentaneoyl ethanolamide (EPEA), thereby serving to terminate the signaling functions of these molecules. EPEA promotes dauer formation and may constitute a signal of high nutrient availability. Breakdown of EPEA may promote lifespan extension when nutrient availability is high (PubMed:21562563). Facilitates axon regeneration after injury by degradating inhibitory compounds such as AEA (PubMed:23072806).2 Publications

Catalytic activityi

Anandamide + H2O = arachidonic acid + ethanolamine.
Oleamide + H2O = oleic acid + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei139 – 1391Charge relay systemBy similarity
Active sitei214 – 2141Charge relay systemBy similarity
Binding sitei214 – 2141SubstrateBy similarity
Active sitei238 – 2381Acyl-ester intermediateBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid amide hydrolase 1 (EC:3.5.1.99)
Gene namesi
Name:faah-1
ORF Names:B0218.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiB0218.1a; CE06684; WBGene00015047; faah-1.
B0218.1b; CE33351; WBGene00015047; faah-1.

Pathology & Biotechi

Disruption phenotypei

Viable. Reduction (50 percent) in the frequency of motoneuron axon regeneration in young adults but not in L4 larvae.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1414Sequence AnalysisAdd
BLAST
Chaini15 – 572558Fatty acid amide hydrolase 1PRO_0000421279Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ17449.
PRIDEiQ17449.

Expressioni

Tissue specificityi

Expressed in the pharynx, some pharyngeal neurons, the posterior intestine and anal depressor muscles.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi6239.B0218.1a.1.

Structurei

3D structure databases

ProteinModelPortaliQ17449.
SMRiQ17449. Positions 34-570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni235 – 2384Substrate bindingBy similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili32 – 6332Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the amidase family.Curated

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiCOG0154.
GeneTreeiENSGT00550000074673.
HOGENOMiHOG000016500.
InParanoidiQ17449.
KOiK15528.
OMAiANDGPMA.
OrthoDBiEOG72JWG0.
PhylomeDBiQ17449.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q17449-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIFYLVLLVL GAIAFYVHFS NNRKKLIERL EIVAQRRRDD LSKNVEQARK
60 70 80 90 100
AADKLDTQRR DWIGSLDFEQ LRDELQRGHV TCVEAIRAYF HKAILAHEKT
110 120 130 140 150
NAVTCFILDA ERQAEELDEQ AKLPYYVKPP LFGVPLSLKE CLKVKGYDTT
160 170 180 190 200
RGFVQDAYHP ATEDSIQVEH YKKLGLIPFC QTNVPQSLLS YNCSNPLFGT
210 220 230 240 250
TTNPYDSTRT CGGSSGGEGA LIGAGGSLIG IGTDVGGSVR IPCHFTGTAG
260 270 280 290 300
IKPSKMRFAH RGGGASVPGK PLIDANDGPM AKDVKTNVEF LRNVWGDIDF
310 320 330 340 350
QSDRDPYCPP VHWNESVYSS EKKLRVGYYI DDGWFTPTPA LQRAVLESKK
360 370 380 390 400
HLEAAGHTVI PFYPPRLPSV MQLYFRAVCL DGGQYVLNKL LKDIIEPTIR
410 420 430 440 450
FQVTLWMVPV WIQRILSYPV SLVFPRMGML MQSLTRDTFE LREAYADIEA
460 470 480 490 500
YREEFVGLMM KDNLDVILCP ASIMPAPQHD IPSKVVSGVS YTCLYNLLDF
510 520 530 540 550
GAGVVPVTAV SKSDEEKLIN EYPETDKWYQ ITKKATLGAV GMPIGVQVAA
560 570
PPYREEAVLR TMREIEIAVT GK
Length:572
Mass (Da):64,001
Last modified:November 1, 1996 - v1
Checksum:i4CE781426865D6E9
GO
Isoform b (identifier: Q17449-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     176-240: Missing.

Show »
Length:507
Mass (Da):57,581
Checksum:iAC99192C957DD3D6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei176 – 24065Missing in isoform b. CuratedVSP_045382Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080124 Genomic DNA. Translation: CCD61383.1.
FO080124 Genomic DNA. Translation: CCD61384.1.
PIRiT29753.
RefSeqiNP_501368.1. NM_068967.5. [Q17449-1]
NP_872095.1. NM_182295.3. [Q17449-2]
UniGeneiCel.17636.

Genome annotation databases

EnsemblMetazoaiB0218.1a.1; B0218.1a.1; WBGene00015047. [Q17449-1]
B0218.1a.2; B0218.1a.2; WBGene00015047. [Q17449-1]
B0218.1a.3; B0218.1a.3; WBGene00015047. [Q17449-1]
B0218.1a.4; B0218.1a.4; WBGene00015047. [Q17449-1]
GeneIDi177613.
KEGGicel:CELE_B0218.1.
UCSCiB0218.1a.1. c. elegans. [Q17449-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080124 Genomic DNA. Translation: CCD61383.1.
FO080124 Genomic DNA. Translation: CCD61384.1.
PIRiT29753.
RefSeqiNP_501368.1. NM_068967.5. [Q17449-1]
NP_872095.1. NM_182295.3. [Q17449-2]
UniGeneiCel.17636.

3D structure databases

ProteinModelPortaliQ17449.
SMRiQ17449. Positions 34-570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.B0218.1a.1.

Proteomic databases

PaxDbiQ17449.
PRIDEiQ17449.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiB0218.1a.1; B0218.1a.1; WBGene00015047. [Q17449-1]
B0218.1a.2; B0218.1a.2; WBGene00015047. [Q17449-1]
B0218.1a.3; B0218.1a.3; WBGene00015047. [Q17449-1]
B0218.1a.4; B0218.1a.4; WBGene00015047. [Q17449-1]
GeneIDi177613.
KEGGicel:CELE_B0218.1.
UCSCiB0218.1a.1. c. elegans. [Q17449-1]

Organism-specific databases

CTDi177613.
WormBaseiB0218.1a; CE06684; WBGene00015047; faah-1.
B0218.1b; CE33351; WBGene00015047; faah-1.

Phylogenomic databases

eggNOGiCOG0154.
GeneTreeiENSGT00550000074673.
HOGENOMiHOG000016500.
InParanoidiQ17449.
KOiK15528.
OMAiANDGPMA.
OrthoDBiEOG72JWG0.
PhylomeDBiQ17449.

Miscellaneous databases

NextBioi897594.
PROiQ17449.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  2. "N-acylethanolamine signalling mediates the effect of diet on lifespan in Caenorhabditis elegans."
    Lucanic M., Held J.M., Vantipalli M.C., Klang I.M., Graham J.B., Gibson B.W., Lithgow G.J., Gill M.S.
    Nature 473:226-229(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  3. "Endocannabinoid-Goalpha signalling inhibits axon regeneration in Caenorhabditis elegans by antagonizing Gqalpha-PKC-JNK signalling."
    Pastuhov S.I., Fujiki K., Nix P., Kanao S., Bastiani M., Matsumoto K., Hisamoto N.
    Nat. Commun. 3:1136-1136(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiFAAH1_CAEEL
AccessioniPrimary (citable) accession number: Q17449
Secondary accession number(s): H2KY70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: November 1, 1996
Last modified: July 22, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.