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Reviewed, UniProtKB/Swiss-Prot Q17446 (PMK1_CAEEL)

Last modified June 16, 2009. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Mitogen-activated protein kinase pmk-1
    EC=2.7.11.24
Alternative name(s):
    p38 MAP kinase 1
    Stress-activated protein kinase pmk-1
Gene names
Name: pmk-1
ORF Names: B0218.3
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Functions downstream of the MAPKK sek-1 and the MAPKKK nsy-1 as the MAP kinase required for pathogen resistance. Ref.1 Ref.3

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. Ref.1

Cofactor

Magnesium. Ref.1

Enzyme regulation

Activated by phosphorylation on threonine and tyrosine. Inhibited by pyridinyl-imidazole related compounds. Ref.1

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 377377Mitogen-activated protein kinase pmk-1
PRO_0000186303

Regions

Domain35 – 319285Protein kinase
Nucleotide binding41 – 499ATP By similarity UniProtKB Q16539
Motif191 – 1933TXY

Sites

Active site1791Proton acceptor By similarity UniProtKB Q16539
Binding site641ATP By similarity UniProtKB Q16539

Amino acid modifications

Modified residue1911Phosphothreonine By similarity UniProtKB Q16539
Modified residue1931Phosphotyrosine By similarity UniProtKB Q16539

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Sequences

Sequence LengthMass (Da)Tools
Q17446-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F20BC395B38EFD03

FASTA37743,919
        10         20         30         40         50         60 
MFPQTTMDHI LHPTPREGYY VVELNRSVWV VPNYYINLTP IGTGAYGTVC AAECTRSGTR 

        70         80         90        100        110        120 
VAIKKFNRPF QSIIHARRTY RELRLLRCMC HENIIDLLDV FTPNENVNDI EDVYFVSMLM 

       130        140        150        160        170        180 
GADLSNILKI QRLNDDHIQF LVYQILRGLK YIHSADIIHR DLKPSNIAVN EDCELKILDF 

       190        200        210        220        230        240 
GLARQTDSEM TGYVATRWYR APEIMLNWMH YTQTVDVWSV GCILAELITG KTLFPGSDHI 

       250        260        270        280        290        300 
DQLTRIMSVT GTPDEEFLKK ISSEEARNYI RNLPKMTRRD FKRLFAQATP QAIDLLEKML 

       310        320        330        340        350        360 
HLDPDRRPTA KEAMEHEYLA AYHDETDEPI AEEMDLNDDV RADTIDEWKK IIWEEISDFQ 

       370 
KNVAFADEEE DEEKMES

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References

« Hide 'large scale' references
[1]"Isolation and characterization of pmk-(1-3): three p38 homologs in Caenorhabditis elegans."
Berman K., McKay J., Avery L., Cobb M.
Mol. Cell Biol. Res. Commun. 4:337-344(2001) [PubMed: 11703092] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ENZYME REGULATION.
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[3]"A conserved p38 MAP kinase pathway in Caenorhabditis elegans innate immunity."
Kim D.H., Feinbaum R., Alloing G., Emerson F.E., Garsin D.A., Inoue H., Tanaka-Hino M., Hisamoto N., Matsumoto K., Tan M.-W., Ausubel F.M.
Science 297:623-626(2002) [PubMed: 12142542] [Abstract]
Cited for: FUNCTION.
Strain: Bristol N2.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U58752 Genomic DNA. Translation: AAB00664.1.
PIRT29750.
RefSeqNP_501365.1.
UniGeneCel.12534

3D structure databases

HSSPHSSP built from PDB template 1KV2 based on UniProtKB Q16539.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:26892N.
IntActQ17446. 23 interactions.

Genome annotation databases

EnsemblB0218.3. Caenorhabditis elegans. [Contig view]
GeneID191743.
KEGGcel:B0218.3.
NMPDRfig|6239.3.peg.14161.

Organism-specific databases

WormBaseWBGene00004055. pmk-1.
WormPepB0218.3. CE06686. [WorfDB]

Phylogenomic databases

OMAQ17446. IRNLPKM.

Enzyme and pathway databases

BRENDA2.7.11.24. 672.

Gene expression databases

ArrayExpressQ17446.

Family and domain databases

InterProIPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01773. P38MAPKINASE.
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. False negative.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio950180.

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Entry information

Entry namePMK1_CAEEL
AccessionPrimary (citable) accession number: Q17446
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: November 1, 1996
Last modified: June 16, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents