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Q17446 (PMK1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase pmk-1

EC=2.7.11.24
Alternative name(s):
Stress-activated protein kinase pmk-1
p38 MAP kinase 1
Gene names
Name:pmk-1
ORF Names:B0218.3
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Functions downstream of the MAPKK sek-1 and the MAPKKK nsy-1 as the MAP kinase required for pathogen resistance. Ref.1 Ref.3

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. Ref.1

Cofactor

Magnesium. Ref.1

Enzyme regulation

Activated by phosphorylation on threonine and tyrosine. Inhibited by pyridinyl-imidazole related compounds. Ref.1

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response to Gram-negative bacterium

Inferred from mutant phenotype Ref.3PubMed 19454349. Source: WormBase

hyperosmotic response

Inferred from genetic interaction PubMed 10393177. Source: WormBase

innate immune response

Inferred from mutant phenotype PubMed 12526744PubMed 19454349. Source: WormBase

intracellular signal transduction

Inferred from direct assay Ref.1. Source: UniProtKB

p38MAPK cascade

Inferred from mutant phenotype Ref.3. Source: WormBase

peptidyl-serine phosphorylation

Inferred from direct assay PubMed 16166371. Source: WormBase

positive regulation of protein localization to nucleus

Inferred from mutant phenotype PubMed 16166371. Source: WormBase

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 19454349. Source: WormBase

positive regulation of transcription from RNA polymerase II promoter involved in defense response to Gram-negative bacterium

Inferred from mutant phenotype PubMed 17888400. Source: WormBase

programmed cell death

Inferred from mutant phenotype PubMed 12526744. Source: WormBase

protein phosphorylation

Inferred from direct assay Ref.1PubMed 20369020. Source: WormBase

response to osmotic stress

Inferred from direct assay Ref.1. Source: UniProtKB

response to reactive oxygen species

Inferred from expression pattern PubMed 16166371. Source: WormBase

response to superoxide

Inferred from expression pattern PubMed 16166371. Source: WormBase

   Cellular_componentcytosol

Inferred from direct assay PubMed 20133945. Source: WormBase

nucleus

Inferred from direct assay PubMed 20133945. Source: WormBase

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from direct assay Ref.1. Source: UniProtKB

protein kinase activity

Inferred from direct assay Ref.1PubMed 20369020. Source: WormBase

protein serine/threonine kinase activity

Inferred from direct assay PubMed 16166371. Source: WormBase

transcription factor binding

Inferred from physical interaction PubMed 20369020. Source: WormBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 377377Mitogen-activated protein kinase pmk-1
PRO_0000186303

Regions

Domain35 – 319285Protein kinase
Nucleotide binding41 – 499ATP By similarity UniProtKB Q16539
Motif191 – 1933TXY

Sites

Active site1791Proton acceptor By similarity UniProtKB Q16539
Binding site641ATP By similarity UniProtKB Q16539

Amino acid modifications

Modified residue1911Phosphothreonine By similarity UniProtKB Q16539
Modified residue1931Phosphotyrosine By similarity UniProtKB Q16539

Sequences

Sequence LengthMass (Da)Tools
Q17446 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F20BC395B38EFD03

FASTA37743,919
        10         20         30         40         50         60 
MFPQTTMDHI LHPTPREGYY VVELNRSVWV VPNYYINLTP IGTGAYGTVC AAECTRSGTR 

        70         80         90        100        110        120 
VAIKKFNRPF QSIIHARRTY RELRLLRCMC HENIIDLLDV FTPNENVNDI EDVYFVSMLM 

       130        140        150        160        170        180 
GADLSNILKI QRLNDDHIQF LVYQILRGLK YIHSADIIHR DLKPSNIAVN EDCELKILDF 

       190        200        210        220        230        240 
GLARQTDSEM TGYVATRWYR APEIMLNWMH YTQTVDVWSV GCILAELITG KTLFPGSDHI 

       250        260        270        280        290        300 
DQLTRIMSVT GTPDEEFLKK ISSEEARNYI RNLPKMTRRD FKRLFAQATP QAIDLLEKML 

       310        320        330        340        350        360 
HLDPDRRPTA KEAMEHEYLA AYHDETDEPI AEEMDLNDDV RADTIDEWKK IIWEEISDFQ 

       370 
KNVAFADEEE DEEKMES 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of pmk-(1-3): three p38 homologs in Caenorhabditis elegans."
Berman K., McKay J., Avery L., Cobb M.
Mol. Cell Biol. Res. Commun. 4:337-344(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ENZYME REGULATION.
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[3]"A conserved p38 MAP kinase pathway in Caenorhabditis elegans innate immunity."
Kim D.H., Feinbaum R., Alloing G., Emerson F.E., Garsin D.A., Inoue H., Tanaka-Hino M., Hisamoto N., Matsumoto K., Tan M.-W., Ausubel F.M.
Science 297:623-626(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FO080124 Genomic DNA. Translation: CCD61386.1.
PIRT29750.
RefSeqNP_501365.1. NM_068964.3.
UniGeneCel.12534.

3D structure databases

ProteinModelPortalQ17446.
SMRQ17446. Positions 16-359.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid56238. 22 interactions.
DIPDIP-26892N.
IntActQ17446. 17 interactions.
MINTMINT-1037719.
STRING6239.B0218.3.

Proteomic databases

PaxDbQ17446.
PRIDEQ17446.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaB0218.3; B0218.3; WBGene00004055.
GeneID191743.
KEGGcel:CELE_B0218.3.
UCSCB0218.3. c. elegans.

Organism-specific databases

CTD191743.
WormBaseB0218.3; CE06686; WBGene00004055; pmk-1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000074271.
HOGENOMHOG000233024.
InParanoidQ17446.
KOK04441.
OMAHEYLAAY.
OrthoDBEOG7PCJGV.
PhylomeDBQ17446.

Enzyme and pathway databases

SignaLinkQ17446.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01773. P38MAPKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio950180.
PROQ17446.

Entry information

Entry namePMK1_CAEEL
AccessionPrimary (citable) accession number: Q17446
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase