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Q17446

- PMK1_CAEEL

UniProt

Q17446 - PMK1_CAEEL

Protein

Mitogen-activated protein kinase pmk-1

Gene

pmk-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Functions downstream of the MAPKK sek-1 and the MAPKKK nsy-1 as the MAP kinase required for pathogen resistance.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.1 Publication

    Cofactori

    Magnesium.1 Publication

    Enzyme regulationi

    Activated by phosphorylation on threonine and tyrosine. Inhibited by pyridinyl-imidazole related compounds.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei64 – 641ATPPROSITE-ProRule annotation
    Active sitei179 – 1791Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi41 – 499ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB
    3. protein kinase activity Source: WormBase
    4. protein serine/threonine kinase activity Source: WormBase
    5. transcription factor binding Source: WormBase

    GO - Biological processi

    1. defense response to Gram-negative bacterium Source: WormBase
    2. hyperosmotic response Source: WormBase
    3. innate immune response Source: WormBase
    4. intracellular signal transduction Source: UniProtKB
    5. p38MAPK cascade Source: WormBase
    6. peptidyl-serine phosphorylation Source: WormBase
    7. positive regulation of protein localization to nucleus Source: WormBase
    8. positive regulation of transcription from RNA polymerase II promoter Source: WormBase
    9. positive regulation of transcription from RNA polymerase II promoter involved in defense response to Gram-negative bacterium Source: WormBase
    10. programmed cell death Source: WormBase
    11. protein phosphorylation Source: WormBase
    12. response to osmotic stress Source: UniProtKB
    13. response to reactive oxygen species Source: WormBase
    14. response to superoxide Source: WormBase

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SignaLinkiQ17446.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase pmk-1 (EC:2.7.11.24)
    Alternative name(s):
    Stress-activated protein kinase pmk-1
    p38 MAP kinase 1
    Gene namesi
    Name:pmk-1
    ORF Names:B0218.3
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome IV

    Organism-specific databases

    WormBaseiB0218.3; CE06686; WBGene00004055; pmk-1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: WormBase
    2. nucleus Source: WormBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 377377Mitogen-activated protein kinase pmk-1PRO_0000186303Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei191 – 1911PhosphothreonineBy similarity
    Modified residuei193 – 1931PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ17446.
    PRIDEiQ17446.

    Interactioni

    Protein-protein interaction databases

    BioGridi56238. 22 interactions.
    DIPiDIP-26892N.
    IntActiQ17446. 17 interactions.
    MINTiMINT-1037719.
    STRINGi6239.B0218.3.

    Structurei

    3D structure databases

    ProteinModelPortaliQ17446.
    SMRiQ17446. Positions 16-359.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 319285Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi191 – 1933TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000074271.
    HOGENOMiHOG000233024.
    InParanoidiQ17446.
    KOiK04441.
    OMAiHEYLAAY.
    OrthoDBiEOG7PCJGV.
    PhylomeDBiQ17446.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008352. MAPK_p38.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01773. P38MAPKINASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q17446-1 [UniParc]FASTAAdd to Basket

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    MFPQTTMDHI LHPTPREGYY VVELNRSVWV VPNYYINLTP IGTGAYGTVC    50
    AAECTRSGTR VAIKKFNRPF QSIIHARRTY RELRLLRCMC HENIIDLLDV 100
    FTPNENVNDI EDVYFVSMLM GADLSNILKI QRLNDDHIQF LVYQILRGLK 150
    YIHSADIIHR DLKPSNIAVN EDCELKILDF GLARQTDSEM TGYVATRWYR 200
    APEIMLNWMH YTQTVDVWSV GCILAELITG KTLFPGSDHI DQLTRIMSVT 250
    GTPDEEFLKK ISSEEARNYI RNLPKMTRRD FKRLFAQATP QAIDLLEKML 300
    HLDPDRRPTA KEAMEHEYLA AYHDETDEPI AEEMDLNDDV RADTIDEWKK 350
    IIWEEISDFQ KNVAFADEEE DEEKMES 377
    Length:377
    Mass (Da):43,919
    Last modified:November 1, 1996 - v1
    Checksum:iF20BC395B38EFD03
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080124 Genomic DNA. Translation: CCD61386.1.
    PIRiT29750.
    RefSeqiNP_501365.1. NM_068964.3.
    UniGeneiCel.12534.

    Genome annotation databases

    EnsemblMetazoaiB0218.3; B0218.3; WBGene00004055.
    GeneIDi191743.
    KEGGicel:CELE_B0218.3.
    UCSCiB0218.3. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080124 Genomic DNA. Translation: CCD61386.1 .
    PIRi T29750.
    RefSeqi NP_501365.1. NM_068964.3.
    UniGenei Cel.12534.

    3D structure databases

    ProteinModelPortali Q17446.
    SMRi Q17446. Positions 16-359.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 56238. 22 interactions.
    DIPi DIP-26892N.
    IntActi Q17446. 17 interactions.
    MINTi MINT-1037719.
    STRINGi 6239.B0218.3.

    Proteomic databases

    PaxDbi Q17446.
    PRIDEi Q17446.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai B0218.3 ; B0218.3 ; WBGene00004055 .
    GeneIDi 191743.
    KEGGi cel:CELE_B0218.3.
    UCSCi B0218.3. c. elegans.

    Organism-specific databases

    CTDi 191743.
    WormBasei B0218.3 ; CE06686 ; WBGene00004055 ; pmk-1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000074271.
    HOGENOMi HOG000233024.
    InParanoidi Q17446.
    KOi K04441.
    OMAi HEYLAAY.
    OrthoDBi EOG7PCJGV.
    PhylomeDBi Q17446.

    Enzyme and pathway databases

    SignaLinki Q17446.

    Miscellaneous databases

    NextBioi 950180.
    PROi Q17446.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008352. MAPK_p38.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01773. P38MAPKINASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of pmk-(1-3): three p38 homologs in Caenorhabditis elegans."
      Berman K., McKay J., Avery L., Cobb M.
      Mol. Cell Biol. Res. Commun. 4:337-344(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ENZYME REGULATION.
      Strain: Bristol N2.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    3. Cited for: FUNCTION.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiPMK1_CAEEL
    AccessioniPrimary (citable) accession number: Q17446
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3