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Protein

Polyprenol reductase

Gene

B0024.13

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism (By similarity).By similarity

Catalytic activityi

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.

Pathway:iprotein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_333882. Synthesis of Dolichyl-phosphate.
REACT_346418. Androgen biosynthesis.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprenol reductase (EC:1.3.1.94)
Gene namesi
ORF Names:B0024.13
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiB0024.13; CE35376; WBGene00007102.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei12 – 3221HelicalSequence AnalysisAdd
BLAST
Transmembranei72 – 9221HelicalSequence AnalysisAdd
BLAST
Transmembranei114 – 13421HelicalSequence AnalysisAdd
BLAST
Transmembranei151 – 17121HelicalSequence AnalysisAdd
BLAST
Transmembranei184 – 20421HelicalSequence AnalysisAdd
BLAST
Transmembranei242 – 26221HelicalSequence AnalysisAdd
BLAST
Transmembranei270 – 29021HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Polyprenol reductasePRO_0000398653Add
BLAST

Proteomic databases

PaxDbiQ17428.

Interactioni

Protein-protein interaction databases

STRINGi6239.B0024.13.

Structurei

3D structure databases

ProteinModelPortaliQ17428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG330066.
GeneTreeiENSGT00500000044920.
HOGENOMiHOG000018885.
InParanoidiQ17428.
KOiK12345.
OMAiLANFWSS.
OrthoDBiEOG72ZCFT.
PhylomeDBiQ17428.

Family and domain databases

InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q17428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDRLWEVRQ ALPLYLLVST LGLAISCCFT LICPHVCRLI PALTTYGKAA
60 70 80 90 100
DQQEDNSLVE KISVPKKWFK HFYAIGLLTL FICLHTVHSL IYNPNYLHPV
110 120 130 140 150
VLKILATLTR SYSIPPITPS TSILALLLIS LHVARRLYET IFVSVYSDSR
160 170 180 190 200
MNLFHYAVGI VHYIILPISI MCETQGVASK LPQLHVSIDD ISLTQWAGAV
210 220 230 240 250
LFWICNWKQH QLAEQIANTR KGPRGLIRNY AYGICFGGWF NLVSCPHFLF
260 270 280 290 300
EICIYLSLFL VIPDAYVYRF IIMFVCINQT FAALITHSWY HKTFPKYPKS

RKALIPYVL
Length:309
Mass (Da):35,430
Last modified:July 5, 2004 - v2
Checksum:i1D4F125A25B3A9EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71178 Genomic DNA. Translation: CAA94885.2.
PIRiT18648.
RefSeqiNP_001256209.1. NM_001269280.1.
UniGeneiCel.9469.

Genome annotation databases

EnsemblMetazoaiB0024.13; B0024.13; WBGene00007102.
GeneIDi179438.
KEGGicel:CELE_B0024.13.
UCSCiB0024.13a. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71178 Genomic DNA. Translation: CAA94885.2.
PIRiT18648.
RefSeqiNP_001256209.1. NM_001269280.1.
UniGeneiCel.9469.

3D structure databases

ProteinModelPortaliQ17428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.B0024.13.

Proteomic databases

PaxDbiQ17428.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiB0024.13; B0024.13; WBGene00007102.
GeneIDi179438.
KEGGicel:CELE_B0024.13.
UCSCiB0024.13a. c. elegans.

Organism-specific databases

CTDi179438.
WormBaseiB0024.13; CE35376; WBGene00007102.

Phylogenomic databases

eggNOGiNOG330066.
GeneTreeiENSGT00500000044920.
HOGENOMiHOG000018885.
InParanoidiQ17428.
KOiK12345.
OMAiLANFWSS.
OrthoDBiEOG72ZCFT.
PhylomeDBiQ17428.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_333882. Synthesis of Dolichyl-phosphate.
REACT_346418. Androgen biosynthesis.

Miscellaneous databases

NextBioi905390.
PROiQ17428.

Family and domain databases

InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiPORED_CAEEL
AccessioniPrimary (citable) accession number: Q17428
Secondary accession number(s): Q7JLP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 5, 2004
Last modified: July 22, 2015
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.