Q17428 (PORED_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polyprenol reductase
EC=1.3.1.94

Gene names

ORF Names:

B0024.13

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism By similarity.
Catalytic activity	Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the steroid 5-alpha reductase family. Polyprenol reductase subfamily.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dolichol metabolic process Inferred from sequence or structural similarity. Source: UniProtKB dolichol-linked oligosaccharide biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB polyprenol catabolic process Inferred from sequence or structural similarity. Source: UniProtKB protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	oxidoreductase activity, acting on the CH-CH group of donors Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Polyprenol reductase		PRO_0000398653
Regions
	Transmembrane	12 – 32	21	Helical; Potential
	Transmembrane	72 – 92	21	Helical; Potential
	Transmembrane	114 – 134	21	Helical; Potential
	Transmembrane	151 – 171	21	Helical; Potential
	Transmembrane	184 – 204	21	Helical; Potential
	Transmembrane	242 – 262	21	Helical; Potential
	Transmembrane	270 – 290	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q17428 [UniParc]. Last modified July 5, 2004. Version 2. Checksum: 1D4F125A25B3A9EC	FASTA	309	35,430
	10 20 30 40 50 60 MLDRLWEVRQ ALPLYLLVST LGLAISCCFT LICPHVCRLI PALTTYGKAA DQQEDNSLVE 70 80 90 100 110 120 KISVPKKWFK HFYAIGLLTL FICLHTVHSL IYNPNYLHPV VLKILATLTR SYSIPPITPS 130 140 150 160 170 180 TSILALLLIS LHVARRLYET IFVSVYSDSR MNLFHYAVGI VHYIILPISI MCETQGVASK 190 200 210 220 230 240 LPQLHVSIDD ISLTQWAGAV LFWICNWKQH QLAEQIANTR KGPRGLIRNY AYGICFGGWF 250 260 270 280 290 300 NLVSCPHFLF EICIYLSLFL VIPDAYVYRF IIMFVCINQT FAALITHSWY HKTFPKYPKS RKALIPYVL

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71178 Genomic DNA. Translation: CAA94885.2.
PIR	T18648.
RefSeq	NP_001256209.1. NM_001269280.1.
3D structure databases
ProteinModelPortal	Q17428.
ModBase	Search...
Protein-protein interaction databases
STRING	6239.B0024.13b.
Proteomic databases
PaxDb	Q17428.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	B0024.13; B0024.13; B0024.13.
GeneID	179438.
KEGG	cel:CELE_B0024.13.
UCSC	B0024.13a. c. elegans.
Organism-specific databases
CTD	179438.
WormBase	B0024.13; CE35376; WBGene00007102.
Phylogenomic databases
eggNOG	NOG330066.
GeneTree	ENSGT00500000044920.
HOGENOM	HOG000018885.
InParanoid	Q7JLP4.
OMA	THKWYLQ.
Enzyme and pathway databases
UniPathway	UPA00378.
Family and domain databases
InterPro	IPR001104. 3-oxo-5_a-steroid_4-DH_C. [Graphical view]
Pfam	PF02544. Steroid_dh. 1 hit. [Graphical view]
PROSITE	PS50244. S5A_REDUCTASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	905390.

Entry information

Entry name

PORED_CAEEL

Accession

Primary (citable) accession number: Q17428
Secondary accession number(s): Q7JLP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project