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Protein

Cullin-2

Gene

cul-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Positive cell-cycle regulator that is required at two distinct points in the cell cycle; the G1-to-S-phase transition and mitosis. Also required for proper cytoskeletal movement and mitotic chromosome condensation. Core component of multiple cullin-RING-based CBC (Cul2-ElonginB-ElonginC) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the CBC complex depends on the variable substrate recognition component (By similarity). May function in ubiquitin-mediated degradation of CKIs to target cki-1 for degradation. CBC(zif-1) may ensure germline precursor cell asymmetry by targeting germline proteins for destruction if expressed in non-germline cells. CBC(fem-1) directs ubiquitination of tra-1.By similarity4 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • anaphase-promoting complex-dependent catabolic process Source: WormBase
  • anterior/posterior axis specification, embryo Source: WormBase
  • asymmetric protein localization Source: WormBase
  • chromosome condensation Source: UniProtKB-KW
  • germ-line stem cell division Source: WormBase
  • male germ-line sex determination Source: WormBase
  • male somatic sex determination Source: WormBase
  • meiotic spindle elongation Source: WormBase
  • mitotic nuclear division Source: UniProtKB-KW
  • positive regulation of metaphase/anaphase transition of meiotic cell cycle Source: WormBase
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: GO_Central
  • sister chromosome movement towards spindle pole involved in meiotic sister chromatid segregation Source: WormBase
  • transcription factor catabolic process Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell cycle, Cell division, DNA condensation, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-CEL-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-2
Short name:
CUL-2
Gene namesi
Name:cul-2
ORF Names:ZK520.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiZK520.4a; CE32774; WBGene00000837; cul-2.
ZK520.4b; CE32775; WBGene00000837; cul-2.
ZK520.4c; CE32776; WBGene00000837; cul-2.
ZK520.4d; CE36259; WBGene00000837; cul-2.

Subcellular locationi

GO - Cellular componenti

  • cullin-RING ubiquitin ligase complex Source: GO_Central
  • cytoplasm Source: WormBase
  • nucleus Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001197811 – 850Cullin-2Add BLAST850

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki795Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)By similarity

Post-translational modificationi

Neddylated; which enhances the ubiquitination activity of CBC (Cul2-ElonginB-ElonginC) E3 ubiquitin-protein ligase complexes.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ17390.
PaxDbiQ17390.
PeptideAtlasiQ17390.
PRIDEiQ17390.

Expressioni

Tissue specificityi

In adults, highly expressed in meiotic cells and oocytes. In larvae, expressed in many proliferating cell types: P cells during the L1 stage; seam cells when they divide at every molt; vulval and somatic gonad cells in late L3 and L4 stages; and intestinal cells throughout larval development.1 Publication

Developmental stagei

Expressed both maternally and zygotically. Highest levels in embryos and lower levels in larvae and adults.1 Publication

Gene expression databases

BgeeiWBGene00000837.

Interactioni

Subunit structurei

Component of multiple CBC (Cul2-ElonginB-ElonginC) E3 ubiquitin-protein ligase complexes formed of cul-2, elb-1, elc-1, rbx-1 and a variable substrate recognition component (By similarity). Component of the CBC(fem-1) E3 ubiquitin-protein ligase complex with fem-1, fem-2 and fem-3. Probable component of an CBC(zif-1) E3 ubiquitin-protein ligase including cul-2, elc-1, rbx-1 and zif-1. Part of an E3 ubiquitin-protein ligase complex including cul-2, elc-1 and zyg-11.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
elc-1Q9BKS12EBI-1811231,EBI-300574
zer-1Q2WF592EBI-1811231,EBI-1811352
zyg-11P215413EBI-1811231,EBI-314647

GO - Molecular functioni

Protein-protein interaction databases

BioGridi41972. 6 interactors.
IntActiQ17390. 9 interactors.
MINTiMINT-4507382.
STRINGi6239.ZK520.4a.

Structurei

3D structure databases

ProteinModelPortaliQ17390.
SMRiQ17390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2284. Eukaryota.
COG5647. LUCA.
GeneTreeiENSGT00760000119212.
HOGENOMiHOG000176713.
InParanoidiQ17390.
KOiK03870.
OMAiMPTQFVE.
OrthoDBiEOG091G024O.
PhylomeDBiQ17390.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR016159. Cullin_repeat-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
[Graphical view]
SMARTiSM00182. CULLIN. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF74788. SSF74788. 1 hit.
SSF75632. SSF75632. 1 hit.
PROSITEiPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q17390-1) [UniParc]FASTAAdd to basket
Also known as: a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTFNIFLTFS TISPKIFSVT STGNRSEVHR PQAKLASNTQ PTSNRTTLGR
60 70 80 90 100
RKAISDDNFP VVEPILKKIR PPRPQVMYSL KPKVVEFDKV WVQLRPSIID
110 120 130 140 150
IINLRPITNV QWHHKFSDVY DICVSIPTPL SERLYNEVKA CIQEHVRQKR
160 170 180 190 200
QDIVDVDPDL LLQEYHKMWR VFHEGAIFIH RLFGYLNKQF VKQKRCTDLD
210 220 230 240 250
NFAQYAAFLQ IPDVKEIGCL ALEIWKEDLV KTILPQLVKL LLIAIDNDRK
260 270 280 290 300
GNFPHIANEV SGVINSFVKM EETDFDVVPA EGARYKARES TTAFYQESFE
310 320 330 340 350
KPLLTDTEQY YSALAQKMLT DLSCSEYMEQ VIVLLEQEEM RAKKYLHESS
360 370 380 390 400
VEKVITLCQK VMIKAHKDKL HAVCHDLITN EENKDLRNMY RLLKPIQAGL
410 420 430 440 450
SVMVKEFEEY VKKKGLEAVS RLTGENVPQQ FVENVLRVYN KFNDMKTAVF
460 470 480 490 500
MDDGEFSSGL DKALQGVVNS KEPGQSVPKA SERLARYTDG LLKKSTKGLS
510 520 530 540 550
ETDLEAKLDS AIVIFRYIED KDIFQKFYSK MLANRLIAST SISMDAEELM
560 570 580 590 600
INKLKQACGY EFTSKLSRMF TDIGLSQELS NNFDKHIADI KTVQPDVKFV
610 620 630 640 650
PTQTMILQAG SWPLNAPQLS TNSNNQTAQD VANFHLPRIL QPVIQEFEKF
660 670 680 690 700
YTGKHNGRKL TWLFNMSQGD VRLTYLDKQY VAQMYVYQMA ALLCFERRDA
710 720 730 740 750
ILVKDIGEEI GVSGDYLLKT IRTILDVTLL TCDDQNLTAD SLVRLNMSMT
760 770 780 790 800
SKRMKFRLQA PQVNKAVEKE QEAVANTVSQ DRKYYMECAI VRIMKTRKVL
810 820 830 840 850
KHNALVTEIM DQTKGRFSPD VPFIKKSIED LIEKMYIQRT DQNDEYQYLA
Length:850
Mass (Da):97,970
Last modified:September 21, 2011 - v3
Checksum:i7D9F4340B9127019
GO
Isoform 2 (identifier: Q17390-2) [UniParc]FASTAAdd to basket
Also known as: b

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MASSTKVSLRR
     2-70: Missing.

Note: No experimental confirmation available.
Show »
Length:791
Mass (Da):91,343
Checksum:i8993DF21905F0738
GO
Isoform 3 (identifier: Q17390-3) [UniParc]FASTAAdd to basket
Also known as: c

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Note: No experimental confirmation available.
Show »
Length:774
Mass (Da):89,450
Checksum:iF722B5585D52550B
GO
Isoform 4 (identifier: Q17390-4) [UniParc]FASTAAdd to basket
Also known as: d

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.
     606-638: Missing.
     777-777: T → TFQ

Show »
Length:743
Mass (Da):86,140
Checksum:iBA2A2F27550AB7D3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti131S → I in AAC47121 (PubMed:8681378).Curated1
Sequence conflicti479 – 480KA → NG in AAC47121 (PubMed:8681378).Curated2
Sequence conflicti680Y → H in AAC47121 (PubMed:8681378).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0417871 – 76Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST76
Alternative sequenceiVSP_0417881M → MASSTKVSLRR in isoform 2. Curated1
Alternative sequenceiVSP_0417892 – 70Missing in isoform 2. CuratedAdd BLAST69
Alternative sequenceiVSP_041790606 – 638Missing in isoform 4. 1 PublicationAdd BLAST33
Alternative sequenceiVSP_041791777T → TFQ in isoform 4. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58084 mRNA. Translation: AAC47121.1.
Z92822 Genomic DNA. Translation: CAB07302.3.
Z92822 Genomic DNA. Translation: CAB70188.2.
Z92822 Genomic DNA. Translation: CAD18893.2.
Z92822 Genomic DNA. Translation: CAD45612.3.
PIRiC88618.
T27884.
RefSeqiNP_001023007.1. NM_001027836.2. [Q17390-3]
NP_001023008.1. NM_001027837.1. [Q17390-2]
NP_001023009.1. NM_001027838.2. [Q17390-4]
NP_499825.3. NM_067424.4. [Q17390-1]
UniGeneiCel.21341.

Genome annotation databases

EnsemblMetazoaiZK520.4a; ZK520.4a; WBGene00000837. [Q17390-1]
GeneIDi176806.
KEGGicel:CELE_ZK520.4.
UCSCiZK520.4a. c. elegans.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58084 mRNA. Translation: AAC47121.1.
Z92822 Genomic DNA. Translation: CAB07302.3.
Z92822 Genomic DNA. Translation: CAB70188.2.
Z92822 Genomic DNA. Translation: CAD18893.2.
Z92822 Genomic DNA. Translation: CAD45612.3.
PIRiC88618.
T27884.
RefSeqiNP_001023007.1. NM_001027836.2. [Q17390-3]
NP_001023008.1. NM_001027837.1. [Q17390-2]
NP_001023009.1. NM_001027838.2. [Q17390-4]
NP_499825.3. NM_067424.4. [Q17390-1]
UniGeneiCel.21341.

3D structure databases

ProteinModelPortaliQ17390.
SMRiQ17390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41972. 6 interactors.
IntActiQ17390. 9 interactors.
MINTiMINT-4507382.
STRINGi6239.ZK520.4a.

Proteomic databases

EPDiQ17390.
PaxDbiQ17390.
PeptideAtlasiQ17390.
PRIDEiQ17390.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZK520.4a; ZK520.4a; WBGene00000837. [Q17390-1]
GeneIDi176806.
KEGGicel:CELE_ZK520.4.
UCSCiZK520.4a. c. elegans.

Organism-specific databases

CTDi176806.
WormBaseiZK520.4a; CE32774; WBGene00000837; cul-2.
ZK520.4b; CE32775; WBGene00000837; cul-2.
ZK520.4c; CE32776; WBGene00000837; cul-2.
ZK520.4d; CE36259; WBGene00000837; cul-2.

Phylogenomic databases

eggNOGiKOG2284. Eukaryota.
COG5647. LUCA.
GeneTreeiENSGT00760000119212.
HOGENOMiHOG000176713.
InParanoidiQ17390.
KOiK03870.
OMAiMPTQFVE.
OrthoDBiEOG091G024O.
PhylomeDBiQ17390.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-CEL-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.

Miscellaneous databases

PROiQ17390.

Gene expression databases

BgeeiWBGene00000837.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR016159. Cullin_repeat-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
[Graphical view]
SMARTiSM00182. CULLIN. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF74788. SSF74788. 1 hit.
SSF75632. SSF75632. 1 hit.
PROSITEiPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCUL2_CAEEL
AccessioniPrimary (citable) accession number: Q17390
Secondary accession number(s): O46021
, Q8I4A7, Q8WQ96, Q9NA22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 21, 2011
Last modified: November 30, 2016
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.