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Protein

Cullin-2

Gene

cul-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Positive cell-cycle regulator that is required at two distinct points in the cell cycle; the G1-to-S-phase transition and mitosis. Also required for proper cytoskeletal movement and mitotic chromosome condensation. Core component of multiple cullin-RING-based CBC (Cul2-ElonginB-ElonginC) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the CBC complex depends on the variable substrate recognition component (By similarity). May function in ubiquitin-mediated degradation of CKIs to target cki-1 for degradation. CBC(zif-1) may ensure germline precursor cell asymmetry by targeting germline proteins for destruction if expressed in non-germline cells. CBC(fem-1) directs ubiquitination of tra-1.By similarity4 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • anaphase-promoting complex-dependent catabolic process Source: WormBase
  • anterior/posterior axis specification, embryo Source: WormBase
  • chromosome condensation Source: UniProtKB-KW
  • germ-line stem cell division Source: WormBase
  • male germ-line sex determination Source: WormBase
  • male somatic sex determination Source: WormBase
  • meiotic spindle elongation Source: WormBase
  • positive regulation of metaphase/anaphase transition of meiotic cell cycle Source: WormBase
  • protein localization Source: WormBase
  • protein ubiquitination Source: UniProtKB-UniPathway
  • sister chromosome movement towards spindle pole involved in meiotic sister chromatid segregation Source: WormBase
  • transcription factor catabolic process Source: WormBase

Keywordsi

Molecular functionDevelopmental protein
Biological processCell cycle, Cell division, DNA condensation, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-CEL-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-CEL-8951664 Neddylation
R-CEL-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-2
Short name:
CUL-2
Gene namesi
Name:cul-2
ORF Names:ZK520.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiZK520.4a ; CE32774 ; WBGene00000837 ; cul-2
ZK520.4b ; CE32775 ; WBGene00000837 ; cul-2
ZK520.4c ; CE32776 ; WBGene00000837 ; cul-2
ZK520.4d ; CE36259 ; WBGene00000837 ; cul-2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001197811 – 850Cullin-2Add BLAST850

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki795Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)By similarity

Post-translational modificationi

Neddylated; which enhances the ubiquitination activity of CBC (Cul2-ElonginB-ElonginC) E3 ubiquitin-protein ligase complexes.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ17390
PaxDbiQ17390
PeptideAtlasiQ17390
PRIDEiQ17390

Expressioni

Tissue specificityi

In adults, highly expressed in meiotic cells and oocytes. In larvae, expressed in many proliferating cell types: P cells during the L1 stage; seam cells when they divide at every molt; vulval and somatic gonad cells in late L3 and L4 stages; and intestinal cells throughout larval development.1 Publication

Developmental stagei

Expressed both maternally and zygotically. Highest levels in embryos and lower levels in larvae and adults.1 Publication

Gene expression databases

BgeeiWBGene00000837

Interactioni

Subunit structurei

Component of multiple CBC (Cul2-ElonginB-ElonginC) E3 ubiquitin-protein ligase complexes formed of cul-2, elb-1, elc-1, rbx-1 and a variable substrate recognition component (By similarity). Component of the CBC(fem-1) E3 ubiquitin-protein ligase complex with fem-1, fem-2 and fem-3. Probable component of an CBC(zif-1) E3 ubiquitin-protein ligase including cul-2, elc-1, rbx-1 and zif-1. Part of an E3 ubiquitin-protein ligase complex including cul-2, elc-1 and zyg-11.By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi41972, 6 interactors
IntActiQ17390, 9 interactors
MINTiQ17390
STRINGi6239.ZK520.4a

Structurei

3D structure databases

ProteinModelPortaliQ17390
SMRiQ17390
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2284 Eukaryota
COG5647 LUCA
GeneTreeiENSGT00760000119212
HOGENOMiHOG000176713
InParanoidiQ17390
KOiK03870
OMAiMPTQFVE
OrthoDBiEOG091G024O
PhylomeDBiQ17390

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR016157 Cullin_CS
IPR016158 Cullin_homology
IPR036317 Cullin_homology_sf
IPR001373 Cullin_N
IPR019559 Cullin_neddylation_domain
IPR016159 Cullin_repeat-like_dom_sf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00888 Cullin, 1 hit
PF10557 Cullin_Nedd8, 1 hit
SMARTiView protein in SMART
SM00182 CULLIN, 1 hit
SM00884 Cullin_Nedd8, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF74788 SSF74788, 1 hit
SSF75632 SSF75632, 1 hit
PROSITEiView protein in PROSITE
PS01256 CULLIN_1, 1 hit
PS50069 CULLIN_2, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q17390-1) [UniParc]FASTAAdd to basket
Also known as: a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTFNIFLTFS TISPKIFSVT STGNRSEVHR PQAKLASNTQ PTSNRTTLGR
60 70 80 90 100
RKAISDDNFP VVEPILKKIR PPRPQVMYSL KPKVVEFDKV WVQLRPSIID
110 120 130 140 150
IINLRPITNV QWHHKFSDVY DICVSIPTPL SERLYNEVKA CIQEHVRQKR
160 170 180 190 200
QDIVDVDPDL LLQEYHKMWR VFHEGAIFIH RLFGYLNKQF VKQKRCTDLD
210 220 230 240 250
NFAQYAAFLQ IPDVKEIGCL ALEIWKEDLV KTILPQLVKL LLIAIDNDRK
260 270 280 290 300
GNFPHIANEV SGVINSFVKM EETDFDVVPA EGARYKARES TTAFYQESFE
310 320 330 340 350
KPLLTDTEQY YSALAQKMLT DLSCSEYMEQ VIVLLEQEEM RAKKYLHESS
360 370 380 390 400
VEKVITLCQK VMIKAHKDKL HAVCHDLITN EENKDLRNMY RLLKPIQAGL
410 420 430 440 450
SVMVKEFEEY VKKKGLEAVS RLTGENVPQQ FVENVLRVYN KFNDMKTAVF
460 470 480 490 500
MDDGEFSSGL DKALQGVVNS KEPGQSVPKA SERLARYTDG LLKKSTKGLS
510 520 530 540 550
ETDLEAKLDS AIVIFRYIED KDIFQKFYSK MLANRLIAST SISMDAEELM
560 570 580 590 600
INKLKQACGY EFTSKLSRMF TDIGLSQELS NNFDKHIADI KTVQPDVKFV
610 620 630 640 650
PTQTMILQAG SWPLNAPQLS TNSNNQTAQD VANFHLPRIL QPVIQEFEKF
660 670 680 690 700
YTGKHNGRKL TWLFNMSQGD VRLTYLDKQY VAQMYVYQMA ALLCFERRDA
710 720 730 740 750
ILVKDIGEEI GVSGDYLLKT IRTILDVTLL TCDDQNLTAD SLVRLNMSMT
760 770 780 790 800
SKRMKFRLQA PQVNKAVEKE QEAVANTVSQ DRKYYMECAI VRIMKTRKVL
810 820 830 840 850
KHNALVTEIM DQTKGRFSPD VPFIKKSIED LIEKMYIQRT DQNDEYQYLA
Length:850
Mass (Da):97,970
Last modified:September 21, 2011 - v3
Checksum:i7D9F4340B9127019
GO
Isoform 2 (identifier: Q17390-2) [UniParc]FASTAAdd to basket
Also known as: b

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MASSTKVSLRR
     2-70: Missing.

Note: No experimental confirmation available.
Show »
Length:791
Mass (Da):91,343
Checksum:i8993DF21905F0738
GO
Isoform 3 (identifier: Q17390-3) [UniParc]FASTAAdd to basket
Also known as: c

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Note: No experimental confirmation available.
Show »
Length:774
Mass (Da):89,450
Checksum:iF722B5585D52550B
GO
Isoform 4 (identifier: Q17390-4) [UniParc]FASTAAdd to basket
Also known as: d

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.
     606-638: Missing.
     777-777: T → TFQ

Show »
Length:743
Mass (Da):86,140
Checksum:iBA2A2F27550AB7D3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti131S → I in AAC47121 (PubMed:8681378).Curated1
Sequence conflicti479 – 480KA → NG in AAC47121 (PubMed:8681378).Curated2
Sequence conflicti680Y → H in AAC47121 (PubMed:8681378).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0417871 – 76Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST76
Alternative sequenceiVSP_0417881M → MASSTKVSLRR in isoform 2. Curated1
Alternative sequenceiVSP_0417892 – 70Missing in isoform 2. CuratedAdd BLAST69
Alternative sequenceiVSP_041790606 – 638Missing in isoform 4. 1 PublicationAdd BLAST33
Alternative sequenceiVSP_041791777T → TFQ in isoform 4. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58084 mRNA Translation: AAC47121.1
Z92822 Genomic DNA Translation: CAB07302.3
Z92822 Genomic DNA Translation: CAB70188.2
Z92822 Genomic DNA Translation: CAD18893.2
Z92822 Genomic DNA Translation: CAD45612.3
PIRiC88618
T27884
RefSeqiNP_001023007.1, NM_001027836.2 [Q17390-3]
NP_001023008.1, NM_001027837.1 [Q17390-2]
NP_001023009.1, NM_001027838.2 [Q17390-4]
NP_499825.3, NM_067424.4
UniGeneiCel.21341

Genome annotation databases

EnsemblMetazoaiZK520.4a; ZK520.4a; WBGene00000837 [Q17390-1]
GeneIDi176806
KEGGicel:CELE_ZK520.4
UCSCiZK520.4a c. elegans

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCUL2_CAEEL
AccessioniPrimary (citable) accession number: Q17390
Secondary accession number(s): O46021
, Q8I4A7, Q8WQ96, Q9NA22
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 21, 2011
Last modified: April 25, 2018
This is version 130 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health