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Protein

Cullin-1

Gene

cul-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Required for developmentally programmed transitions from the G1 phase of the cell cycle to the G0 phase or the apoptotic pathway.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • determination of adult lifespan Source: UniProtKB
  • embryo development ending in birth or egg hatching Source: WormBase
  • negative regulation of apoptotic process Source: WormBase
  • negative regulation of cell proliferation Source: WormBase
  • negative regulation of centrosome duplication Source: UniProtKB
  • negative regulation of DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
  • negative regulation of mitotic cell cycle Source: WormBase
  • nematode larval development Source: WormBase
  • positive regulation of apoptotic process Source: WormBase
  • protein ubiquitination Source: UniProtKB-UniPathway
  • regulation of cell size Source: WormBase
  • regulation of protein stability Source: UniProtKB
  • reproduction Source: WormBase
  • response to starvation Source: UniProtKB
  • ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-CEL-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-CEL-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-CEL-69231. Cyclin D associated events in G1.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-1
Short name:
CUL-1
Alternative name(s):
Abnormal cell lineage protein 19
Gene namesi
Name:cul-1
Synonyms:lin-19
ORF Names:D2045.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiD2045.6; CE29089; WBGene00000836; cul-1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 780780Cullin-1PRO_0000119780Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki724 – 724Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ17389.
PaxDbiQ17389.
PRIDEiQ17389.

Expressioni

Tissue specificityi

Ubiquitous.

Developmental stagei

Highest levels in embryos and lower levels in larvae and adults.

Interactioni

Subunit structurei

Component of an SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex composed of cul-1, fsn-1, rpm-1 and skr-1 (PubMed:15208641). Interacts with Skp1-related proteins skr-1, skr-2, skr-3, skr-4, skr-7, skr-8, skr-9 and skr-10 (PubMed:11864566).2 Publications

Protein-protein interaction databases

BioGridi41658. 8 interactions.
STRINGi6239.D2045.6.

Structurei

3D structure databases

ProteinModelPortaliQ17389.
SMRiQ17389. Positions 16-780.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2166. Eukaryota.
COG5647. LUCA.
GeneTreeiENSGT00760000119212.
HOGENOMiHOG000176713.
InParanoidiQ17389.
KOiK03347.
OMAiKDNANMY.
OrthoDBiEOG7X3QQG.
PhylomeDBiQ17389.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR016159. Cullin_repeat-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
[Graphical view]
SMARTiSM00182. CULLIN. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF74788. SSF74788. 1 hit.
SSF75632. SSF75632. 1 hit.
PROSITEiPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q17389-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNSEPRRMT CDSEVVWKKL QDGLDVAYRR ENMAPKDYMT LYTSVYDYCT
60 70 80 90 100
SITLSTSRRD GEDGRAESST PARTAGADFV GHEMYQRVEE YVKAYVIAVC
110 120 130 140 150
EKGAELSGED LLKYYTTEWE NFRISSKVMD GIFAYLNRHW IRRELDEGHE
160 170 180 190 200
NIYMVYTLAL VVWKRNLFND LKDKVIDAML ELIRSERTGS MINSRYISGV
210 220 230 240 250
VECLVELGVD DSETDAKKDA ETKKLAVYKE FFEVKFLEAT RGFYTQEAAN
260 270 280 290 300
FLSNGGNVTD YMIKVETRLN QEDDRCQLYL NSSTKTPLAT CCESVLISNQ
310 320 330 340 350
LDFLQRHFGG LLVDKRDDDL SRMFKLCDRV PNGLDELRKS LENHIAKEGH
360 370 380 390 400
QALERVAMEA ATDAKLYVKT LLEVHERYQS LVNRSFKNEP GFMQSLDKAA
410 420 430 440 450
TSFINNNAVT KRAPPQAQLT KSAELLARYC DQLLRKSSKM PDEAELEELQ
460 470 480 490 500
TKIMVVFKYI DDKDVFSKFY TKMFSKRLIS ELSASDEAEA NFITKLKSMC
510 520 530 540 550
GYEYTARLSK MVNDTQVSKD LTADFKEKKA DMLGQKSVEF NVLVLSSGSW
560 570 580 590 600
PTFPTTPITL PQQLSKTIEI FGQFYNEKFN GRRLTWVYSQ SRGEITSTAF
610 620 630 640 650
PKKYVFTATT AQMCTMLLFN EQDSYTVEQI AAATKMDEKS APAIVGSLIK
660 670 680 690 700
NLVLKADTEL QKEDEVPMTA TVSLNKAYMN KKVRVDLSKF TMKQDAVRDT
710 720 730 740 750
ENVQKNVEED RKSVISACIV RIMKTRKRVQ HQQLMTEVIT QLSGRFKPKV
760 770 780
EMIKRCIGSL IEKEYMLRTE GQKDLYEYLA
Length:780
Mass (Da):89,527
Last modified:November 1, 1997 - v1
Checksum:i38A5C2EE0A7CC881
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58083 mRNA. Translation: AAC47120.1.
Z35639 Genomic DNA. Translation: CAA84695.2.
PIRiT20365.
RefSeqiNP_499309.1. NM_066908.4.
UniGeneiCel.6661.

Genome annotation databases

EnsemblMetazoaiD2045.6; D2045.6; WBGene00000836.
GeneIDi176466.
KEGGicel:CELE_D2045.6.
UCSCiD2045.6. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58083 mRNA. Translation: AAC47120.1.
Z35639 Genomic DNA. Translation: CAA84695.2.
PIRiT20365.
RefSeqiNP_499309.1. NM_066908.4.
UniGeneiCel.6661.

3D structure databases

ProteinModelPortaliQ17389.
SMRiQ17389. Positions 16-780.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41658. 8 interactions.
STRINGi6239.D2045.6.

Proteomic databases

EPDiQ17389.
PaxDbiQ17389.
PRIDEiQ17389.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiD2045.6; D2045.6; WBGene00000836.
GeneIDi176466.
KEGGicel:CELE_D2045.6.
UCSCiD2045.6. c. elegans.

Organism-specific databases

CTDi176466.
WormBaseiD2045.6; CE29089; WBGene00000836; cul-1.

Phylogenomic databases

eggNOGiKOG2166. Eukaryota.
COG5647. LUCA.
GeneTreeiENSGT00760000119212.
HOGENOMiHOG000176713.
InParanoidiQ17389.
KOiK03347.
OMAiKDNANMY.
OrthoDBiEOG7X3QQG.
PhylomeDBiQ17389.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-CEL-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-CEL-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-CEL-69231. Cyclin D associated events in G1.

Miscellaneous databases

NextBioi892698.
PROiQ17389.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR016159. Cullin_repeat-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
[Graphical view]
SMARTiSM00182. CULLIN. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF74788. SSF74788. 1 hit.
SSF75632. SSF75632. 1 hit.
PROSITEiPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family."
    Kipreos E.T., Lander L.E., Wing J.P., He W.W., Hedgecock E.M.
    Cell 85:829-839(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Multiple Skp1-related proteins in Caenorhabditis elegans: diverse patterns of interaction with Cullins and F-box proteins."
    Yamanaka A., Yada M., Imaki H., Koga M., Ohshima Y., Nakayama K.
    Curr. Biol. 12:267-275(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SKR-1; SKR-2; SKR-3; SKR-4; SKR-7; SKR-8; SKR-9 AND SKR-10.
  4. "An SCF-like ubiquitin ligase complex that controls presynaptic differentiation."
    Liao E.H., Hung W., Abrams B., Zhen M.
    Nature 430:345-350(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RPM-1; FSN-1 AND SKR-1.
  5. "Regulation of Caenorhabditis elegans lifespan by a proteasomal E3 ligase complex."
    Ghazi A., Henis-Korenblit S., Kenyon C.
    Proc. Natl. Acad. Sci. U.S.A. 104:5947-5952(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCUL1_CAEEL
AccessioniPrimary (citable) accession number: Q17389
Secondary accession number(s): Q18985
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.