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Protein

Ubiquitin carboxyl-terminal hydrolase 14

Gene

usp-14

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei111 – 1111NucleophilePROSITE-ProRule annotation
Active sitei409 – 4091Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.A36.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 14 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 14
Ubiquitin thioesterase 14
Ubiquitin-specific-processing protease 14
Gene namesi
Name:usp-14
Synonyms:tgt-1
ORF Names:C13B4.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiC13B4.2; CE15615; WBGene00006856; usp-14.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489Ubiquitin carboxyl-terminal hydrolase 14PRO_0000080640Add
BLAST

Proteomic databases

PaxDbiQ17361.

Interactioni

Protein-protein interaction databases

STRINGi6239.C13B4.2.1.

Structurei

3D structure databases

ProteinModelPortaliQ17361.
SMRiQ17361. Positions 4-80, 101-458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini102 – 458357USPAdd
BLAST

Sequence similaritiesi

Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5207.
GeneTreeiENSGT00390000009615.
HOGENOMiHOG000202292.
InParanoidiQ17361.
KOiK11843.
OMAiDWGNLKI.
OrthoDBiEOG7F7W8H.
PhylomeDBiQ17361.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q17361-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIVNVKWQK EKYVVEVDTS APPMVFKAQL FALTQVVPER QKVVIMGRTL
60 70 80 90 100
GDDDWEGITI KENMTIMMMG SVGEIPKPPT VLEKKQANRD KQAEEISALY
110 120 130 140 150
PCGLANLGNT CYFNSCVQML KEVNELVLKP AEEMRIREHN DRLCHNLATL
160 170 180 190 200
FNSLRDKDRA LRSKGEPIKP FAAILTLSDS FPQFEKFKQQ DANECLVSIM
210 220 230 240 250
SNVTRIYGLS GWNIESLFRI QTETTMKCLE SDEVSEKKVE RNNQLTCYVN
260 270 280 290 300
QDVRFLQTGI KAGFEEEMTR NSEELNRDAK WQKNTQISRL PKYLTVNINR
310 320 330 340 350
FFYKESTKTN AKILKSVQFP MQLDTYDLCS QELKDKLVAR RADIKLEEDA
360 370 380 390 400
KLERELRKKV LDKEQGDKIF DDGVALPTAF EDDAGSNNSG FYDLKGIITH
410 420 430 440 450
KGRSSQDGHY VAWMRSSEDG KWRLFDDEHV TVVDEEAILK TSGGGDWHSA
460 470 480
YVLLYEARVI KQFPELPPAP VPTEVAADTA EPMEVSEKQ
Length:489
Mass (Da):55,878
Last modified:April 16, 2002 - v2
Checksum:i4433F2CC59703A9B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti398 – 3981I → M in AAA74956 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32223 mRNA. Translation: AAA74956.1.
Z81468, Z83236 Genomic DNA. Translation: CAB03876.1.
PIRiT19227.
RefSeqiNP_497006.1. NM_064605.8.
UniGeneiCel.19745.

Genome annotation databases

EnsemblMetazoaiC13B4.2; C13B4.2; WBGene00006856.
GeneIDi175105.
KEGGicel:CELE_C13B4.2.
UCSCiC13B4.2.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32223 mRNA. Translation: AAA74956.1.
Z81468, Z83236 Genomic DNA. Translation: CAB03876.1.
PIRiT19227.
RefSeqiNP_497006.1. NM_064605.8.
UniGeneiCel.19745.

3D structure databases

ProteinModelPortaliQ17361.
SMRiQ17361. Positions 4-80, 101-458.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C13B4.2.1.

Protein family/group databases

MEROPSiC19.A36.

Proteomic databases

PaxDbiQ17361.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC13B4.2; C13B4.2; WBGene00006856.
GeneIDi175105.
KEGGicel:CELE_C13B4.2.
UCSCiC13B4.2.1. c. elegans.

Organism-specific databases

CTDi175105.
WormBaseiC13B4.2; CE15615; WBGene00006856; usp-14.

Phylogenomic databases

eggNOGiCOG5207.
GeneTreeiENSGT00390000009615.
HOGENOMiHOG000202292.
InParanoidiQ17361.
KOiK11843.
OMAiDWGNLKI.
OrthoDBiEOG7F7W8H.
PhylomeDBiQ17361.

Miscellaneous databases

NextBioi886786.
PROiQ17361.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Deshpande K.L., Katze J.R.
    Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiUBP14_CAEEL
AccessioniPrimary (citable) accession number: Q17361
Secondary accession number(s): O45248
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 16, 2002
Last modified: June 24, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Caution

Was originally (Ref. 1) thought to be a queuine tRNA-ribosyltransferase.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.