Q17361 (UBP14_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 14
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 14
Ubiquitin thioesterase 14
Ubiquitin-specific-processing protease 14

Gene names

Name:	usp-14
Synonyms:	tgt-1
ORF Names:	C13B4.2

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
Caution	Was originally (Ref.1) thought to be a queuine tRNA-ribosyltransferase.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Proteasome
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	proteasome complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 489	489	Ubiquitin carboxyl-terminal hydrolase 14		PRO_0000080640
Sites
	Active site	111	1	Nucleophile By similarity
	Active site	409	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	398	1	I → M in AAA74956. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q17361 [UniParc]. Last modified April 16, 2002. Version 2. Checksum: 4433F2CC59703A9B	FASTA	489	55,878
	10 20 30 40 50 60 MPIVNVKWQK EKYVVEVDTS APPMVFKAQL FALTQVVPER QKVVIMGRTL GDDDWEGITI 70 80 90 100 110 120 KENMTIMMMG SVGEIPKPPT VLEKKQANRD KQAEEISALY PCGLANLGNT CYFNSCVQML 130 140 150 160 170 180 KEVNELVLKP AEEMRIREHN DRLCHNLATL FNSLRDKDRA LRSKGEPIKP FAAILTLSDS 190 200 210 220 230 240 FPQFEKFKQQ DANECLVSIM SNVTRIYGLS GWNIESLFRI QTETTMKCLE SDEVSEKKVE 250 260 270 280 290 300 RNNQLTCYVN QDVRFLQTGI KAGFEEEMTR NSEELNRDAK WQKNTQISRL PKYLTVNINR 310 320 330 340 350 360 FFYKESTKTN AKILKSVQFP MQLDTYDLCS QELKDKLVAR RADIKLEEDA KLERELRKKV 370 380 390 400 410 420 LDKEQGDKIF DDGVALPTAF EDDAGSNNSG FYDLKGIITH KGRSSQDGHY VAWMRSSEDG 430 440 450 460 470 480 KWRLFDDEHV TVVDEEAILK TSGGGDWHSA YVLLYEARVI KQFPELPPAP VPTEVAADTA EPMEVSEKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Deshpande K.L., Katze J.R. Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U32223 mRNA. Translation: AAA74956.1. Z81468, Z83236 Genomic DNA. Translation: CAB03876.1. Z83236, Z81468 Genomic DNA. Translation: CAB05785.1.
PIR	T19227.
RefSeq	NP_497006.1. NM_064605.8.
3D structure databases
ProteinModelPortal	Q17361.
SMR	Q17361. Positions 4-80, 101-458.
ModBase	Search...
Protein family/group databases
MEROPS	C19.A36.
Proteomic databases
PaxDb	Q17361.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C13B4.2.1; C13B4.2.1; C13B4.2. C13B4.2.2; C13B4.2.2; C13B4.2.
GeneID	175105.
KEGG	cel:CELE_C13B4.2.
UCSC	C13B4.2.1. c. elegans.
Organism-specific databases
CTD	175105.
WormBase	C13B4.2; CE15615; WBGene00006856; usp-14.
Phylogenomic databases
eggNOG	COG5207.
GeneTree	ENSGT00390000009615.
HOGENOM	HOG000202292.
InParanoid	Q17361.
KO	K11843.
OMA	YGPRRIE.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR000626. Ubiquitin. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
SMART	SM00213. UBQ. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	886786.

Entry information

Entry name

UBP14_CAEEL

Accession

Primary (citable) accession number: Q17361
Secondary accession number(s): O45248

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	April 16, 2002
Last modified:	May 1, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project