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Q17361 (UBP14_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 14

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 14
Ubiquitin thioesterase 14
Ubiquitin-specific-processing protease 14
Gene names
Name:usp-14
Synonyms:tgt-1
ORF Names:C13B4.2
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length489 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sequence similarities

Belongs to the peptidase C19 family. USP14/UBP6 subfamily.

Contains 1 USP domain.

Caution

Was originally (Ref.1) thought to be a queuine tRNA-ribosyltransferase.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentProteasome
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentproteasome complex

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 489489Ubiquitin carboxyl-terminal hydrolase 14
PRO_0000080640

Regions

Domain102 – 458357USP

Sites

Active site1111Nucleophile By similarity
Active site4091Proton acceptor By similarity

Experimental info

Sequence conflict3981I → M in AAA74956. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q17361 [UniParc].

Last modified April 16, 2002. Version 2.
Checksum: 4433F2CC59703A9B

FASTA48955,878
        10         20         30         40         50         60 
MPIVNVKWQK EKYVVEVDTS APPMVFKAQL FALTQVVPER QKVVIMGRTL GDDDWEGITI 

        70         80         90        100        110        120 
KENMTIMMMG SVGEIPKPPT VLEKKQANRD KQAEEISALY PCGLANLGNT CYFNSCVQML 

       130        140        150        160        170        180 
KEVNELVLKP AEEMRIREHN DRLCHNLATL FNSLRDKDRA LRSKGEPIKP FAAILTLSDS 

       190        200        210        220        230        240 
FPQFEKFKQQ DANECLVSIM SNVTRIYGLS GWNIESLFRI QTETTMKCLE SDEVSEKKVE 

       250        260        270        280        290        300 
RNNQLTCYVN QDVRFLQTGI KAGFEEEMTR NSEELNRDAK WQKNTQISRL PKYLTVNINR 

       310        320        330        340        350        360 
FFYKESTKTN AKILKSVQFP MQLDTYDLCS QELKDKLVAR RADIKLEEDA KLERELRKKV 

       370        380        390        400        410        420 
LDKEQGDKIF DDGVALPTAF EDDAGSNNSG FYDLKGIITH KGRSSQDGHY VAWMRSSEDG 

       430        440        450        460        470        480 
KWRLFDDEHV TVVDEEAILK TSGGGDWHSA YVLLYEARVI KQFPELPPAP VPTEVAADTA 


EPMEVSEKQ 

« Hide

References

« Hide 'large scale' references
[1]Deshpande K.L., Katze J.R.
Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U32223 mRNA. Translation: AAA74956.1.
Z81468, Z83236 Genomic DNA. Translation: CAB03876.1.
PIRT19227.
RefSeqNP_497006.1. NM_064605.8.
UniGeneCel.19745.

3D structure databases

ProteinModelPortalQ17361.
SMRQ17361. Positions 4-80, 101-458.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC19.A36.

Proteomic databases

PaxDbQ17361.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaC13B4.2; C13B4.2; WBGene00006856.
GeneID175105.
KEGGcel:CELE_C13B4.2.
UCSCC13B4.2.1. c. elegans.

Organism-specific databases

CTD175105.
WormBaseC13B4.2; CE15615; WBGene00006856; usp-14.

Phylogenomic databases

eggNOGCOG5207.
GeneTreeENSGT00390000009615.
HOGENOMHOG000202292.
InParanoidQ17361.
KOK11843.
OMADWGNLKI.
OrthoDBEOG7F7W8H.
PhylomeDBQ17361.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
SMARTSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMSSF54236. SSF54236. 1 hit.
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio886786.
PROQ17361.

Entry information

Entry nameUBP14_CAEEL
AccessionPrimary (citable) accession number: Q17361
Secondary accession number(s): O45248
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 16, 2002
Last modified: June 11, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase