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Q17353

- PAR3_CAEEL

UniProt

Q17353 - PAR3_CAEEL

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Protein

Partitioning defective protein 3

Gene
par-3, F54E7.3
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

In cooperation with pkc-3, required for establishing cell polarity and regulating spindle orientation in the early embryo. Localization is crucial for recruiting par-6 and pkc-3 to the peripheral apical cortex and restricting par-2 to basolateral surfaces. Necessary for apicobasal and anterior-posterior asymmetries associated with cell adhesion and gastrulation during the first few cycles of embryogenesis, and also for epithelial cell polarity in the distal spermatheca.6 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. asymmetric cell division Source: WormBase
  2. asymmetric protein localization Source: WormBase
  3. cell adhesion Source: UniProtKB
  4. cell differentiation Source: UniProtKB-KW
  5. establishment of cell polarity Source: WormBase
  6. establishment of mitotic spindle localization Source: UniProtKB
  7. establishment of mitotic spindle orientation Source: WormBase
  8. establishment or maintenance of cell polarity Source: UniProtKB
  9. gastrulation Source: UniProtKB
  10. gonadal mesoderm development Source: UniProtKB-KW
  11. gonad development Source: UniProtKB
  12. polarity specification of anterior/posterior axis Source: WormBase
  13. single fertilization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell adhesion, Cell cycle, Cell division, Differentiation, Fertilization, Gastrulation, Gonadal differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Partitioning defective protein 3
Alternative name(s):
Abnormal embryonic partitioning of cytoplasm protein 3
Gene namesi
Name:par-3
ORF Names:F54E7.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiF54E7.3a; CE28449; WBGene00003918; par-3.
F54E7.3b; CE28450; WBGene00003918; par-3.
F54E7.3c; CE39941; WBGene00003918; par-3.

Subcellular locationi

Cytoplasm
Note: Cytoplasmic and cell periphery.1 Publication

GO - Cellular componenti

  1. apical part of cell Source: WormBase
  2. basal part of cell Source: WormBase
  3. cell cortex Source: WormBase
  4. cytoplasm Source: UniProtKB
  5. integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13791379Partitioning defective protein 3PRO_0000185074Add
BLAST

Proteomic databases

PaxDbiQ17353.
PRIDEiQ17353.

Expressioni

Tissue specificityi

Asymmetrically distributed at the periphery of the zygote and in dividing blastomeres of the germline lineage. Coexpressed with par-6; patchy expression observed at the periphery after completion of meiosis I and in meiosis II. On completion of metaphase II, expression is restricted to the anterior 85% of embryo length; this decreases to 55% in embryos between prophase and telophase of the first mitosis. During the first cleavage, expression is detected in the advancing furrow. Transiently coexpressed and colocalized asymmetrically with par-6 and pkc-3, in the developing somatic gonad, including the spermathecal precursor cells of L4 larvae.4 Publications

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Interactioni

Subunit structurei

Required, together with pkc-3, for the localization of par-6; par-6 is involved in localizing/maintaining par-3 at the cell periphery. Interacts with par-6 and pkc-3 for localization at the periphery of anterior cortex of the embryo.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
abu-7O165022EBI-1812329,EBI-328492
CELE_F53A10.2H2L0552EBI-1812329,EBI-2413872
CELE_T11B7.1Q223873EBI-1812329,EBI-320525
pkc-3Q192663EBI-321762,EBI-319158

Protein-protein interaction databases

BioGridi41010. 5 interactions.
DIPiDIP-25317N.
IntActiQ17353. 40 interactions.
MINTiMINT-1103392.

Structurei

3D structure databases

ProteinModelPortaliQ17353.
SMRiQ17353. Positions 69-150, 506-597, 663-758.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini381 – 483103PDZ 1Add
BLAST
Domaini515 – 59985PDZ 2Add
BLAST
Domaini659 – 75092PDZ 3Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili606 – 62621 Reviewed predictionAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi776 – 81944Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the PAR3 family.1 Publication
Contains 3 PDZ (DHR) domains.

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG268288.
GeneTreeiENSGT00740000115461.
HOGENOMiHOG000018783.
InParanoidiQ17353.
KOiK04237.
OMAiQANTQYI.
PhylomeDBiQ17353.

Family and domain databases

Gene3Di2.30.42.10. 3 hits.
InterProiIPR021922. DUF3534.
IPR001478. PDZ.
[Graphical view]
PfamiPF12053. DUF3534. 1 hit.
PF00595. PDZ. 3 hits.
[Graphical view]
SMARTiSM00228. PDZ. 3 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 3 hits.
PROSITEiPS50106. PDZ. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform b1 Publication (identifier: Q17353-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSASSTSSSS TSCPEGGEPS GSCKSSDEGE STLKKRMQQY GIASGYANSS     50
ISTLDRSQYQ SLPLNGTRRV TVQFGRMKIV VPWKESDQTV GQLADAALLR 100
YKKARGMANE DRIHVHRLEC ASDGGILDMD DVLEEVFDLN YDQILAITDE 150
ANGGSTTPTY SQIQKQQHHY AQPLPYARKF DGGPSTPIAS AFGSVTVNHQ 200
AHRAASPYNV GFARSNSRDF APQPTHSKER RDSVVEVSSF DQIPQSGLRV 250
STPKPSRQSE DVIDGKPMNQ PILRSSLRTE ASGSRTEEAT PVKQSRVTLS 300
PEVEKKLAEQ DERKSERRKH YDKNPGRFAR GSDRKSRITD ALLDARDRIA 350
DQLESQNPAE ETKSQMIRVK IDQGPMPGTS LVTFPPIPEK SENEKQLGIE 400
VNAVFDESSE LPGTSEPTKL SSVQIMKIED GGRIAKDGRI RVGDCIVAID 450
GKPVDQMSII RVRASISDLA AVTSRPVTLI INRSLESFLE QESSAKPIQS 500
ALQQANTQYI GHTTVVELIK SSNGFGFTVT GRETAKGERL FYIGTVKPYG 550
VALGHLKSGD RLLEINGTPT GQWTQSEIVE KLKETMVGEK IKFLVSRVSQ 600
SAIMSTSASS ENKENEETLK VVEEEKIPQK LPLPALMTPP VPKDTPALSP 650
SGASRFEIVI PFINGSSSAG LGVSLKARVS KKSNGSKVDC GIFIKNVMHG 700
GAAFKEGGLR VDDRIVGVED IDLEPLDNRE AQAALAKKLK EVGMISSNVR 750
LTISRYNECN PGQISRDLSR ITVDASSPSP SSRMSSHTAP DSLLPSPATR 800
GTSSSGADSS HSRQSSASSA VPAVPARLTE RDSIVSDGTS RNDESELPDS 850
ADPFNREGLG RKSLSEKRGM GAAADPQHIK LFQDIKHQRQ NSAPTSSTQK 900
RSKSQPRSSS QRNYRSPMKL VDLPTTAAAS ASTNSQNLDD SDMLNRRSQS 950
MESINRPVES ILRGTGQIPT GSSSKVQFMQ AASPDQHPFP PGAALLRLKN 1000
EESRSRDKSR RKSMGNPFSA MRNFFGFGSK SRDASPEKTP TESVQLRSVE 1050
RPKSIIDERN NGSSERAPPP LPPHQSQRRG SGGNVFVDYG EPYGLIPQYP 1100
HNTTSGYESY ADSELYDRYA AHRYHPRGGP IIDEDEYIYR QQSTSGNSPI 1150
NTSSYVNYGL PASNAYHVGS RIPPQTSSGS ISKTSGAMRR VYPAEYDEDV 1200
AYHQQIPQQS TRYQQGSGSG RGNADYHHMF NSWFAYTGGG AVGAAPVIKS 1250
SYGSSPVRIA AASAIERGES FVVEPVSGSS ASATDRRGRS TSSGAVASGS 1300
SSTGFQYAAK EKYADARSGK FNGGSTRLFI PRHGGGLSAA AFATNFGGEA 1350
YETRGGGAGG SPSQYRRRDQ GPPHRFPQY 1379
Length:1,379
Mass (Da):149,326
Last modified:November 1, 1996 - v1
Checksum:i3B498E7652CFF17D
GO
Isoform a1 Publication (identifier: Q17353-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1017-1019: Missing.

Note: No experimental confirmation available.

Show »
Length:1,376
Mass (Da):148,994
Checksum:i92CF11545C626856
GO
Isoform c (identifier: Q17353-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-107: MSASSTSSSS...LLRYKKARGM → MHNGRGGRYD...NVTVSKRNFQ
     1017-1019: Missing.

Note: No experimental confirmation available.

Show »
Length:1,340
Mass (Da):145,588
Checksum:i390E4FBB0E9236E0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 107107MSASS…KARGM → MHNGRGGRYDVCPPPPPPPY HFNHVHTPPSKVIVQQQKQQ QKAHREPPPSYPASKMTTTN DNVTVSKRNFQ in isoform c. VSP_034689Add
BLAST
Alternative sequencei1017 – 10193Missing in isoform a and isoform c. 1 PublicationVSP_051597

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25032 mRNA. Translation: AAB18670.1.
FO080172 Genomic DNA. Translation: CCD61764.1.
FO080172 Genomic DNA. Translation: CCD61765.1.
FO080172 Genomic DNA. Translation: CCD61766.1.
RefSeqiNP_001022607.1. NM_001027436.2. [Q17353-1]
NP_001040857.1. NM_001047392.1. [Q17353-3]
NP_498217.2. NM_065816.6. [Q17353-2]
UniGeneiCel.5328.

Genome annotation databases

EnsemblMetazoaiF54E7.3b.1; F54E7.3b.1; WBGene00003918. [Q17353-1]
F54E7.3b.2; F54E7.3b.2; WBGene00003918. [Q17353-1]
GeneIDi175783.
KEGGicel:CELE_F54E7.3.
UCSCiF54E7.3b. c. elegans. [Q17353-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25032 mRNA. Translation: AAB18670.1 .
FO080172 Genomic DNA. Translation: CCD61764.1 .
FO080172 Genomic DNA. Translation: CCD61765.1 .
FO080172 Genomic DNA. Translation: CCD61766.1 .
RefSeqi NP_001022607.1. NM_001027436.2. [Q17353-1 ]
NP_001040857.1. NM_001047392.1. [Q17353-3 ]
NP_498217.2. NM_065816.6. [Q17353-2 ]
UniGenei Cel.5328.

3D structure databases

ProteinModelPortali Q17353.
SMRi Q17353. Positions 69-150, 506-597, 663-758.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 41010. 5 interactions.
DIPi DIP-25317N.
IntActi Q17353. 40 interactions.
MINTi MINT-1103392.

Proteomic databases

PaxDbi Q17353.
PRIDEi Q17353.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai F54E7.3b.1 ; F54E7.3b.1 ; WBGene00003918 . [Q17353-1 ]
F54E7.3b.2 ; F54E7.3b.2 ; WBGene00003918 . [Q17353-1 ]
GeneIDi 175783.
KEGGi cel:CELE_F54E7.3.
UCSCi F54E7.3b. c. elegans. [Q17353-1 ]

Organism-specific databases

CTDi 175783.
WormBasei F54E7.3a ; CE28449 ; WBGene00003918 ; par-3.
F54E7.3b ; CE28450 ; WBGene00003918 ; par-3.
F54E7.3c ; CE39941 ; WBGene00003918 ; par-3.

Phylogenomic databases

eggNOGi NOG268288.
GeneTreei ENSGT00740000115461.
HOGENOMi HOG000018783.
InParanoidi Q17353.
KOi K04237.
OMAi QANTQYI.
PhylomeDBi Q17353.

Miscellaneous databases

NextBioi 889646.

Family and domain databases

Gene3Di 2.30.42.10. 3 hits.
InterProi IPR021922. DUF3534.
IPR001478. PDZ.
[Graphical view ]
Pfami PF12053. DUF3534. 1 hit.
PF00595. PDZ. 3 hits.
[Graphical view ]
SMARTi SM00228. PDZ. 3 hits.
[Graphical view ]
SUPFAMi SSF50156. SSF50156. 3 hits.
PROSITEi PS50106. PDZ. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Asymmetrically distributed PAR-3 protein contributes to cell polarity and spindle alignment in early C. elegans embryos."
    Etemad-Moghadam B., Guo S., Kemphues K.J.
    Cell 83:743-752(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Bristol N2.
    Tissue: Embryo.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "par-6, a gene involved in the establishment of asymmetry in early C. elegans embryos, mediates the asymmetric localization of PAR-3."
    Watts J.L., Etemad-Moghadam B., Guo S., Boyd L., Draper B.W., Mello C.C., Priess J.R., Kemphues K.J.
    Development 122:3133-3140(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PAR-6.
    Strain: Bristol N2.
  4. "Atypical protein kinase C cooperates with PAR-3 to establish embryonic polarity in Caenorhabditis elegans."
    Tabuse Y., Izumi Y., Piano F., Kemphues K.J., Miwa J., Ohno S.
    Development 125:3607-3614(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PKC-3.
  5. "PAR-6 is a conserved PDZ domain-containing protein that colocalizes with PAR-3 in Caenorhabditis elegans embryos."
    Hung T.-J., Kemphues K.J.
    Development 126:127-135(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PAR-6 AND PKC-3, TISSUE SPECIFICITY.
    Strain: Bristol N2.
  6. "C. elegans PAR-3 and PAR-6 are required for apicobasal asymmetries associated with cell adhesion and gastrulation."
    Nance J., Munro E.M., Priess J.R.
    Development 130:5339-5350(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  7. "PAR-3 is required for epithelial cell polarity in the distal spermatheca of C. elegans."
    Aono S., Legouis R., Hoose W.A., Kemphues K.J.
    Development 131:2865-2874(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiPAR3_CAEEL
AccessioniPrimary (citable) accession number: Q17353
Secondary accession number(s): Q27GV0, Q95QE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi