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Q17353 (PAR3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Partitioning defective protein 3
Alternative name(s):
Abnormal embryonic partitioning of cytoplasm protein 3
Gene names
Name:par-3
ORF Names:F54E7.3
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length1379 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

In cooperation with pkc-3, required for establishing cell polarity and regulating spindle orientation in the early embryo. Localization is crucial for recruiting par-6 and pkc-3 to the peripheral apical cortex and restricting par-2 to basolateral surfaces. Necessary for apicobasal and anterior-posterior asymmetries associated with cell adhesion and gastrulation during the first few cycles of embryogenesis, and also for epithelial cell polarity in the distal spermatheca. Ref.1 Ref.3 Ref.4 Ref.5 Ref.6 Ref.7

Subunit structure

Required, together with pkc-3, for the localization of par-6; par-6 is involved in localizing/maintaining par-3 at the cell periphery. Interacts with par-6 and pkc-3 for localization at the periphery of anterior cortex of the embryo. Ref.3 Ref.4 Ref.5

Subcellular location

Cytoplasm. Note: Cytoplasmic and cell periphery. Ref.1

Tissue specificity

Asymmetrically distributed at the periphery of the zygote and in dividing blastomeres of the germline lineage. Coexpressed with par-6; patchy expression observed at the periphery after completion of meiosis I and in meiosis II. On completion of metaphase II, expression is restricted to the anterior 85% of embryo length; this decreases to 55% in embryos between prophase and telophase of the first mitosis. During the first cleavage, expression is detected in the advancing furrow. Transiently coexpressed and colocalized asymmetrically with par-6 and pkc-3, in the developing somatic gonad, including the spermathecal precursor cells of L4 larvae. Ref.1 Ref.5 Ref.6 Ref.7

Developmental stage

Expressed both maternally and zygotically. Ref.1

Sequence similarities

Belongs to the PAR3 family. Ref.1

Contains 3 PDZ (DHR) domains.

Ontologies

Keywords
   Biological processCell adhesion
Cell cycle
Cell division
Differentiation
Fertilization
Gastrulation
Gonadal differentiation
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
Repeat
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processasymmetric cell division

Inferred from mutant phenotype PubMed 7713417. Source: WormBase

asymmetric protein localization

Inferred from mutant phenotype PubMed 9130699. Source: WormBase

cell adhesion

Inferred from mutant phenotype Ref.6. Source: UniProtKB

cell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

establishment of cell polarity

Inferred from mutant phenotype Ref.1. Source: WormBase

establishment of mitotic spindle localization

Inferred from genetic interaction Ref.1. Source: UniProtKB

establishment of mitotic spindle orientation

Inferred from mutant phenotype PubMed 7713417. Source: WormBase

establishment or maintenance of cell polarity

Inferred from genetic interaction Ref.1. Source: UniProtKB

gastrulation

Inferred from mutant phenotype Ref.6. Source: UniProtKB

gonad development

Inferred from mutant phenotype Ref.7. Source: UniProtKB

gonadal mesoderm development

Inferred from electronic annotation. Source: UniProtKB-KW

polarity specification of anterior/posterior axis

Inferred from mutant phenotype PubMed 15716356. Source: WormBase

single fertilization

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentapical part of cell

Inferred from direct assay PubMed 11807031. Source: WormBase

basal part of cell

Inferred from direct assay PubMed 11807031. Source: WormBase

cell cortex

Inferred from direct assay Ref.1. Source: WormBase

cytoplasm

Inferred from direct assay Ref.1. Source: UniProtKB

integral component of membrane

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 21110867. Source: IntAct

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform b Ref.1 (identifier: Q17353-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform a Ref.2 (identifier: Q17353-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1017-1019: Missing.
Note: No experimental confirmation available.
Isoform c (identifier: Q17353-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-107: MSASSTSSSS...LLRYKKARGM → MHNGRGGRYD...NVTVSKRNFQ
     1017-1019: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13791379Partitioning defective protein 3
PRO_0000185074

Regions

Domain381 – 483103PDZ 1
Domain515 – 59985PDZ 2
Domain659 – 75092PDZ 3
Coiled coil606 – 62621 Potential
Compositional bias776 – 81944Ser-rich

Natural variations

Alternative sequence1 – 107107MSASS…KARGM → MHNGRGGRYDVCPPPPPPPY HFNHVHTPPSKVIVQQQKQQ QKAHREPPPSYPASKMTTTN DNVTVSKRNFQ in isoform c.
VSP_034689
Alternative sequence1017 – 10193Missing in isoform a and isoform c. Ref.2
VSP_051597

Sequences

Sequence LengthMass (Da)Tools
Isoform b [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3B498E7652CFF17D

FASTA1,379149,326
        10         20         30         40         50         60 
MSASSTSSSS TSCPEGGEPS GSCKSSDEGE STLKKRMQQY GIASGYANSS ISTLDRSQYQ 

        70         80         90        100        110        120 
SLPLNGTRRV TVQFGRMKIV VPWKESDQTV GQLADAALLR YKKARGMANE DRIHVHRLEC 

       130        140        150        160        170        180 
ASDGGILDMD DVLEEVFDLN YDQILAITDE ANGGSTTPTY SQIQKQQHHY AQPLPYARKF 

       190        200        210        220        230        240 
DGGPSTPIAS AFGSVTVNHQ AHRAASPYNV GFARSNSRDF APQPTHSKER RDSVVEVSSF 

       250        260        270        280        290        300 
DQIPQSGLRV STPKPSRQSE DVIDGKPMNQ PILRSSLRTE ASGSRTEEAT PVKQSRVTLS 

       310        320        330        340        350        360 
PEVEKKLAEQ DERKSERRKH YDKNPGRFAR GSDRKSRITD ALLDARDRIA DQLESQNPAE 

       370        380        390        400        410        420 
ETKSQMIRVK IDQGPMPGTS LVTFPPIPEK SENEKQLGIE VNAVFDESSE LPGTSEPTKL 

       430        440        450        460        470        480 
SSVQIMKIED GGRIAKDGRI RVGDCIVAID GKPVDQMSII RVRASISDLA AVTSRPVTLI 

       490        500        510        520        530        540 
INRSLESFLE QESSAKPIQS ALQQANTQYI GHTTVVELIK SSNGFGFTVT GRETAKGERL 

       550        560        570        580        590        600 
FYIGTVKPYG VALGHLKSGD RLLEINGTPT GQWTQSEIVE KLKETMVGEK IKFLVSRVSQ 

       610        620        630        640        650        660 
SAIMSTSASS ENKENEETLK VVEEEKIPQK LPLPALMTPP VPKDTPALSP SGASRFEIVI 

       670        680        690        700        710        720 
PFINGSSSAG LGVSLKARVS KKSNGSKVDC GIFIKNVMHG GAAFKEGGLR VDDRIVGVED 

       730        740        750        760        770        780 
IDLEPLDNRE AQAALAKKLK EVGMISSNVR LTISRYNECN PGQISRDLSR ITVDASSPSP 

       790        800        810        820        830        840 
SSRMSSHTAP DSLLPSPATR GTSSSGADSS HSRQSSASSA VPAVPARLTE RDSIVSDGTS 

       850        860        870        880        890        900 
RNDESELPDS ADPFNREGLG RKSLSEKRGM GAAADPQHIK LFQDIKHQRQ NSAPTSSTQK 

       910        920        930        940        950        960 
RSKSQPRSSS QRNYRSPMKL VDLPTTAAAS ASTNSQNLDD SDMLNRRSQS MESINRPVES 

       970        980        990       1000       1010       1020 
ILRGTGQIPT GSSSKVQFMQ AASPDQHPFP PGAALLRLKN EESRSRDKSR RKSMGNPFSA 

      1030       1040       1050       1060       1070       1080 
MRNFFGFGSK SRDASPEKTP TESVQLRSVE RPKSIIDERN NGSSERAPPP LPPHQSQRRG 

      1090       1100       1110       1120       1130       1140 
SGGNVFVDYG EPYGLIPQYP HNTTSGYESY ADSELYDRYA AHRYHPRGGP IIDEDEYIYR 

      1150       1160       1170       1180       1190       1200 
QQSTSGNSPI NTSSYVNYGL PASNAYHVGS RIPPQTSSGS ISKTSGAMRR VYPAEYDEDV 

      1210       1220       1230       1240       1250       1260 
AYHQQIPQQS TRYQQGSGSG RGNADYHHMF NSWFAYTGGG AVGAAPVIKS SYGSSPVRIA 

      1270       1280       1290       1300       1310       1320 
AASAIERGES FVVEPVSGSS ASATDRRGRS TSSGAVASGS SSTGFQYAAK EKYADARSGK 

      1330       1340       1350       1360       1370 
FNGGSTRLFI PRHGGGLSAA AFATNFGGEA YETRGGGAGG SPSQYRRRDQ GPPHRFPQY 

« Hide

Isoform a [UniParc].

Checksum: 92CF11545C626856
Show »

FASTA1,376148,994
Isoform c [UniParc].

Checksum: 390E4FBB0E9236E0
Show »

FASTA1,340145,588

References

« Hide 'large scale' references
[1]"Asymmetrically distributed PAR-3 protein contributes to cell polarity and spindle alignment in early C. elegans embryos."
Etemad-Moghadam B., Guo S., Kemphues K.J.
Cell 83:743-752(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: Bristol N2.
Tissue: Embryo.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[3]"par-6, a gene involved in the establishment of asymmetry in early C. elegans embryos, mediates the asymmetric localization of PAR-3."
Watts J.L., Etemad-Moghadam B., Guo S., Boyd L., Draper B.W., Mello C.C., Priess J.R., Kemphues K.J.
Development 122:3133-3140(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PAR-6.
Strain: Bristol N2.
[4]"Atypical protein kinase C cooperates with PAR-3 to establish embryonic polarity in Caenorhabditis elegans."
Tabuse Y., Izumi Y., Piano F., Kemphues K.J., Miwa J., Ohno S.
Development 125:3607-3614(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PKC-3.
[5]"PAR-6 is a conserved PDZ domain-containing protein that colocalizes with PAR-3 in Caenorhabditis elegans embryos."
Hung T.-J., Kemphues K.J.
Development 126:127-135(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PAR-6 AND PKC-3, TISSUE SPECIFICITY.
Strain: Bristol N2.
[6]"C. elegans PAR-3 and PAR-6 are required for apicobasal asymmetries associated with cell adhesion and gastrulation."
Nance J., Munro E.M., Priess J.R.
Development 130:5339-5350(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]"PAR-3 is required for epithelial cell polarity in the distal spermatheca of C. elegans."
Aono S., Legouis R., Hoose W.A., Kemphues K.J.
Development 131:2865-2874(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U25032 mRNA. Translation: AAB18670.1.
FO080172 Genomic DNA. Translation: CCD61764.1.
FO080172 Genomic DNA. Translation: CCD61765.1.
FO080172 Genomic DNA. Translation: CCD61766.1.
RefSeqNP_001022607.1. NM_001027436.2. [Q17353-1]
NP_001040857.1. NM_001047392.1. [Q17353-3]
NP_498217.2. NM_065816.6. [Q17353-2]
UniGeneCel.5328.

3D structure databases

ProteinModelPortalQ17353.
SMRQ17353. Positions 69-150, 506-597, 663-758.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid41010. 5 interactions.
DIPDIP-25317N.
IntActQ17353. 40 interactions.
MINTMINT-1103392.

Proteomic databases

PaxDbQ17353.
PRIDEQ17353.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaF54E7.3b.1; F54E7.3b.1; WBGene00003918. [Q17353-1]
F54E7.3b.2; F54E7.3b.2; WBGene00003918. [Q17353-1]
GeneID175783.
KEGGcel:CELE_F54E7.3.
UCSCF54E7.3b. c. elegans. [Q17353-1]

Organism-specific databases

CTD175783.
WormBaseF54E7.3a; CE28449; WBGene00003918; par-3.
F54E7.3b; CE28450; WBGene00003918; par-3.
F54E7.3c; CE39941; WBGene00003918; par-3.

Phylogenomic databases

eggNOGNOG268288.
GeneTreeENSGT00740000115461.
HOGENOMHOG000018783.
InParanoidQ17353.
KOK04237.
OMAQANTQYI.
PhylomeDBQ17353.

Family and domain databases

Gene3D2.30.42.10. 3 hits.
InterProIPR021922. DUF3534.
IPR001478. PDZ.
[Graphical view]
PfamPF12053. DUF3534. 1 hit.
PF00595. PDZ. 3 hits.
[Graphical view]
SMARTSM00228. PDZ. 3 hits.
[Graphical view]
SUPFAMSSF50156. SSF50156. 3 hits.
PROSITEPS50106. PDZ. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio889646.

Entry information

Entry namePAR3_CAEEL
AccessionPrimary (citable) accession number: Q17353
Secondary accession number(s): Q27GV0, Q95QE9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase