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Q17353

- PAR3_CAEEL

UniProt

Q17353 - PAR3_CAEEL

Protein

Partitioning defective protein 3

Gene

par-3

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    In cooperation with pkc-3, required for establishing cell polarity and regulating spindle orientation in the early embryo. Localization is crucial for recruiting par-6 and pkc-3 to the peripheral apical cortex and restricting par-2 to basolateral surfaces. Necessary for apicobasal and anterior-posterior asymmetries associated with cell adhesion and gastrulation during the first few cycles of embryogenesis, and also for epithelial cell polarity in the distal spermatheca.6 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. asymmetric cell division Source: WormBase
    2. asymmetric protein localization Source: WormBase
    3. cell adhesion Source: UniProtKB
    4. cell differentiation Source: UniProtKB-KW
    5. establishment of cell polarity Source: WormBase
    6. establishment of mitotic spindle localization Source: UniProtKB
    7. establishment of mitotic spindle orientation Source: WormBase
    8. establishment or maintenance of cell polarity Source: UniProtKB
    9. gastrulation Source: UniProtKB
    10. gonadal mesoderm development Source: UniProtKB-KW
    11. gonad development Source: UniProtKB
    12. polarity specification of anterior/posterior axis Source: WormBase
    13. single fertilization Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Cell adhesion, Cell cycle, Cell division, Differentiation, Fertilization, Gastrulation, Gonadal differentiation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Partitioning defective protein 3
    Alternative name(s):
    Abnormal embryonic partitioning of cytoplasm protein 3
    Gene namesi
    Name:par-3Imported
    ORF Names:F54E7.3
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiF54E7.3a; CE28449; WBGene00003918; par-3.
    F54E7.3b; CE28450; WBGene00003918; par-3.
    F54E7.3c; CE39941; WBGene00003918; par-3.

    Subcellular locationi

    Cytoplasm 1 Publication
    Note: Cytoplasmic and cell periphery.

    GO - Cellular componenti

    1. apical part of cell Source: WormBase
    2. basal part of cell Source: WormBase
    3. cell cortex Source: WormBase
    4. cytoplasm Source: UniProtKB
    5. integral component of membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13791379Partitioning defective protein 3PRO_0000185074Add
    BLAST

    Proteomic databases

    PaxDbiQ17353.
    PRIDEiQ17353.

    Expressioni

    Tissue specificityi

    Asymmetrically distributed at the periphery of the zygote and in dividing blastomeres of the germline lineage. Coexpressed with par-6; patchy expression observed at the periphery after completion of meiosis I and in meiosis II. On completion of metaphase II, expression is restricted to the anterior 85% of embryo length; this decreases to 55% in embryos between prophase and telophase of the first mitosis. During the first cleavage, expression is detected in the advancing furrow. Transiently coexpressed and colocalized asymmetrically with par-6 and pkc-3, in the developing somatic gonad, including the spermathecal precursor cells of L4 larvae.4 Publications

    Developmental stagei

    Expressed both maternally and zygotically.1 Publication

    Interactioni

    Subunit structurei

    Required, together with pkc-3, for the localization of par-6; par-6 is involved in localizing/maintaining par-3 at the cell periphery. Interacts with par-6 and pkc-3 for localization at the periphery of anterior cortex of the embryo.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    abu-7O165022EBI-1812329,EBI-328492
    CELE_F53A10.2H2L0552EBI-1812329,EBI-2413872
    CELE_T11B7.1Q223873EBI-1812329,EBI-320525
    pkc-3Q192663EBI-321762,EBI-319158

    Protein-protein interaction databases

    BioGridi41010. 5 interactions.
    DIPiDIP-25317N.
    IntActiQ17353. 40 interactions.
    MINTiMINT-1103392.

    Structurei

    3D structure databases

    ProteinModelPortaliQ17353.
    SMRiQ17353. Positions 69-150, 506-597, 663-758.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini381 – 483103PDZ 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini515 – 59985PDZ 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini659 – 75092PDZ 3PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili606 – 62621Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi776 – 81944Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PAR3 family.1 Publication
    Contains 3 PDZ (DHR) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiNOG268288.
    GeneTreeiENSGT00740000115461.
    HOGENOMiHOG000018783.
    InParanoidiQ17353.
    KOiK04237.
    OMAiQANTQYI.
    PhylomeDBiQ17353.

    Family and domain databases

    Gene3Di2.30.42.10. 3 hits.
    InterProiIPR021922. DUF3534.
    IPR001478. PDZ.
    [Graphical view]
    PfamiPF12053. DUF3534. 1 hit.
    PF00595. PDZ. 3 hits.
    [Graphical view]
    SMARTiSM00228. PDZ. 3 hits.
    [Graphical view]
    SUPFAMiSSF50156. SSF50156. 3 hits.
    PROSITEiPS50106. PDZ. 3 hits.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform b1 Publication (identifier: Q17353-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSASSTSSSS TSCPEGGEPS GSCKSSDEGE STLKKRMQQY GIASGYANSS     50
    ISTLDRSQYQ SLPLNGTRRV TVQFGRMKIV VPWKESDQTV GQLADAALLR 100
    YKKARGMANE DRIHVHRLEC ASDGGILDMD DVLEEVFDLN YDQILAITDE 150
    ANGGSTTPTY SQIQKQQHHY AQPLPYARKF DGGPSTPIAS AFGSVTVNHQ 200
    AHRAASPYNV GFARSNSRDF APQPTHSKER RDSVVEVSSF DQIPQSGLRV 250
    STPKPSRQSE DVIDGKPMNQ PILRSSLRTE ASGSRTEEAT PVKQSRVTLS 300
    PEVEKKLAEQ DERKSERRKH YDKNPGRFAR GSDRKSRITD ALLDARDRIA 350
    DQLESQNPAE ETKSQMIRVK IDQGPMPGTS LVTFPPIPEK SENEKQLGIE 400
    VNAVFDESSE LPGTSEPTKL SSVQIMKIED GGRIAKDGRI RVGDCIVAID 450
    GKPVDQMSII RVRASISDLA AVTSRPVTLI INRSLESFLE QESSAKPIQS 500
    ALQQANTQYI GHTTVVELIK SSNGFGFTVT GRETAKGERL FYIGTVKPYG 550
    VALGHLKSGD RLLEINGTPT GQWTQSEIVE KLKETMVGEK IKFLVSRVSQ 600
    SAIMSTSASS ENKENEETLK VVEEEKIPQK LPLPALMTPP VPKDTPALSP 650
    SGASRFEIVI PFINGSSSAG LGVSLKARVS KKSNGSKVDC GIFIKNVMHG 700
    GAAFKEGGLR VDDRIVGVED IDLEPLDNRE AQAALAKKLK EVGMISSNVR 750
    LTISRYNECN PGQISRDLSR ITVDASSPSP SSRMSSHTAP DSLLPSPATR 800
    GTSSSGADSS HSRQSSASSA VPAVPARLTE RDSIVSDGTS RNDESELPDS 850
    ADPFNREGLG RKSLSEKRGM GAAADPQHIK LFQDIKHQRQ NSAPTSSTQK 900
    RSKSQPRSSS QRNYRSPMKL VDLPTTAAAS ASTNSQNLDD SDMLNRRSQS 950
    MESINRPVES ILRGTGQIPT GSSSKVQFMQ AASPDQHPFP PGAALLRLKN 1000
    EESRSRDKSR RKSMGNPFSA MRNFFGFGSK SRDASPEKTP TESVQLRSVE 1050
    RPKSIIDERN NGSSERAPPP LPPHQSQRRG SGGNVFVDYG EPYGLIPQYP 1100
    HNTTSGYESY ADSELYDRYA AHRYHPRGGP IIDEDEYIYR QQSTSGNSPI 1150
    NTSSYVNYGL PASNAYHVGS RIPPQTSSGS ISKTSGAMRR VYPAEYDEDV 1200
    AYHQQIPQQS TRYQQGSGSG RGNADYHHMF NSWFAYTGGG AVGAAPVIKS 1250
    SYGSSPVRIA AASAIERGES FVVEPVSGSS ASATDRRGRS TSSGAVASGS 1300
    SSTGFQYAAK EKYADARSGK FNGGSTRLFI PRHGGGLSAA AFATNFGGEA 1350
    YETRGGGAGG SPSQYRRRDQ GPPHRFPQY 1379
    Length:1,379
    Mass (Da):149,326
    Last modified:November 1, 1996 - v1
    Checksum:i3B498E7652CFF17D
    GO
    Isoform a (identifier: Q17353-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1017-1019: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:1,376
    Mass (Da):148,994
    Checksum:i92CF11545C626856
    GO
    Isoform c (identifier: Q17353-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-107: MSASSTSSSS...LLRYKKARGM → MHNGRGGRYD...NVTVSKRNFQ
         1017-1019: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,340
    Mass (Da):145,588
    Checksum:i390E4FBB0E9236E0
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 107107MSASS…KARGM → MHNGRGGRYDVCPPPPPPPY HFNHVHTPPSKVIVQQQKQQ QKAHREPPPSYPASKMTTTN DNVTVSKRNFQ in isoform c. CuratedVSP_034689Add
    BLAST
    Alternative sequencei1017 – 10193Missing in isoform a and isoform c. CuratedVSP_051597

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25032 mRNA. Translation: AAB18670.1.
    FO080172 Genomic DNA. Translation: CCD61764.1.
    FO080172 Genomic DNA. Translation: CCD61765.1.
    FO080172 Genomic DNA. Translation: CCD61766.1.
    RefSeqiNP_001022607.1. NM_001027436.2. [Q17353-1]
    NP_001040857.1. NM_001047392.1. [Q17353-3]
    NP_498217.2. NM_065816.6. [Q17353-2]
    UniGeneiCel.5328.

    Genome annotation databases

    EnsemblMetazoaiF54E7.3b.1; F54E7.3b.1; WBGene00003918. [Q17353-1]
    F54E7.3b.2; F54E7.3b.2; WBGene00003918. [Q17353-1]
    GeneIDi175783.
    KEGGicel:CELE_F54E7.3.
    UCSCiF54E7.3b. c. elegans. [Q17353-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25032 mRNA. Translation: AAB18670.1 .
    FO080172 Genomic DNA. Translation: CCD61764.1 .
    FO080172 Genomic DNA. Translation: CCD61765.1 .
    FO080172 Genomic DNA. Translation: CCD61766.1 .
    RefSeqi NP_001022607.1. NM_001027436.2. [Q17353-1 ]
    NP_001040857.1. NM_001047392.1. [Q17353-3 ]
    NP_498217.2. NM_065816.6. [Q17353-2 ]
    UniGenei Cel.5328.

    3D structure databases

    ProteinModelPortali Q17353.
    SMRi Q17353. Positions 69-150, 506-597, 663-758.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 41010. 5 interactions.
    DIPi DIP-25317N.
    IntActi Q17353. 40 interactions.
    MINTi MINT-1103392.

    Proteomic databases

    PaxDbi Q17353.
    PRIDEi Q17353.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai F54E7.3b.1 ; F54E7.3b.1 ; WBGene00003918 . [Q17353-1 ]
    F54E7.3b.2 ; F54E7.3b.2 ; WBGene00003918 . [Q17353-1 ]
    GeneIDi 175783.
    KEGGi cel:CELE_F54E7.3.
    UCSCi F54E7.3b. c. elegans. [Q17353-1 ]

    Organism-specific databases

    CTDi 175783.
    WormBasei F54E7.3a ; CE28449 ; WBGene00003918 ; par-3.
    F54E7.3b ; CE28450 ; WBGene00003918 ; par-3.
    F54E7.3c ; CE39941 ; WBGene00003918 ; par-3.

    Phylogenomic databases

    eggNOGi NOG268288.
    GeneTreei ENSGT00740000115461.
    HOGENOMi HOG000018783.
    InParanoidi Q17353.
    KOi K04237.
    OMAi QANTQYI.
    PhylomeDBi Q17353.

    Miscellaneous databases

    NextBioi 889646.

    Family and domain databases

    Gene3Di 2.30.42.10. 3 hits.
    InterProi IPR021922. DUF3534.
    IPR001478. PDZ.
    [Graphical view ]
    Pfami PF12053. DUF3534. 1 hit.
    PF00595. PDZ. 3 hits.
    [Graphical view ]
    SMARTi SM00228. PDZ. 3 hits.
    [Graphical view ]
    SUPFAMi SSF50156. SSF50156. 3 hits.
    PROSITEi PS50106. PDZ. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Asymmetrically distributed PAR-3 protein contributes to cell polarity and spindle alignment in early C. elegans embryos."
      Etemad-Moghadam B., Guo S., Kemphues K.J.
      Cell 83:743-752(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: Bristol N21 Publication.
      Tissue: Embryo1 Publication.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.
    3. "par-6, a gene involved in the establishment of asymmetry in early C. elegans embryos, mediates the asymmetric localization of PAR-3."
      Watts J.L., Etemad-Moghadam B., Guo S., Boyd L., Draper B.W., Mello C.C., Priess J.R., Kemphues K.J.
      Development 122:3133-3140(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PAR-6.
      Strain: Bristol N21 Publication.
    4. "Atypical protein kinase C cooperates with PAR-3 to establish embryonic polarity in Caenorhabditis elegans."
      Tabuse Y., Izumi Y., Piano F., Kemphues K.J., Miwa J., Ohno S.
      Development 125:3607-3614(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PKC-3.
    5. "PAR-6 is a conserved PDZ domain-containing protein that colocalizes with PAR-3 in Caenorhabditis elegans embryos."
      Hung T.-J., Kemphues K.J.
      Development 126:127-135(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PAR-6 AND PKC-3, TISSUE SPECIFICITY.
      Strain: Bristol N21 Publication.
    6. "C. elegans PAR-3 and PAR-6 are required for apicobasal asymmetries associated with cell adhesion and gastrulation."
      Nance J., Munro E.M., Priess J.R.
      Development 130:5339-5350(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    7. "PAR-3 is required for epithelial cell polarity in the distal spermatheca of C. elegans."
      Aono S., Legouis R., Hoose W.A., Kemphues K.J.
      Development 131:2865-2874(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPAR3_CAEEL
    AccessioniPrimary (citable) accession number: Q17353
    Secondary accession number(s): Q27GV0, Q95QE9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3