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Q17353

- PAR3_CAEEL

UniProt

Q17353 - PAR3_CAEEL

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Protein

Partitioning defective protein 3

Gene

par-3

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

In cooperation with pkc-3, required for establishing cell polarity and regulating spindle orientation in the early embryo. Localization is crucial for recruiting par-6 and pkc-3 to the peripheral apical cortex and restricting par-2 to basolateral surfaces. Necessary for apicobasal and anterior-posterior asymmetries associated with cell adhesion and gastrulation during the first few cycles of embryogenesis, and also for epithelial cell polarity in the distal spermatheca.6 Publications

GO - Biological processi

  1. asymmetric cell division Source: WormBase
  2. asymmetric protein localization Source: WormBase
  3. cell adhesion Source: UniProtKB
  4. cell differentiation Source: UniProtKB-KW
  5. establishment of cell polarity Source: WormBase
  6. establishment of mitotic spindle localization Source: UniProtKB
  7. establishment of mitotic spindle orientation Source: WormBase
  8. establishment or maintenance of cell polarity Source: UniProtKB
  9. gastrulation Source: UniProtKB
  10. gonadal mesoderm development Source: UniProtKB-KW
  11. gonad development Source: UniProtKB
  12. polarity specification of anterior/posterior axis Source: WormBase
  13. single fertilization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell adhesion, Cell cycle, Cell division, Differentiation, Fertilization, Gastrulation, Gonadal differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Partitioning defective protein 3
Alternative name(s):
Abnormal embryonic partitioning of cytoplasm protein 3
Gene namesi
Name:par-3Imported
ORF Names:F54E7.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiF54E7.3a; CE28449; WBGene00003918; par-3.
F54E7.3b; CE28450; WBGene00003918; par-3.
F54E7.3c; CE39941; WBGene00003918; par-3.

Subcellular locationi

Cytoplasm 1 Publication
Note: Cytoplasmic and cell periphery.

GO - Cellular componenti

  1. apical part of cell Source: WormBase
  2. basal part of cell Source: WormBase
  3. cell cortex Source: WormBase
  4. cytoplasm Source: UniProtKB
  5. integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13791379Partitioning defective protein 3PRO_0000185074Add
BLAST

Proteomic databases

PaxDbiQ17353.
PRIDEiQ17353.

Expressioni

Tissue specificityi

Asymmetrically distributed at the periphery of the zygote and in dividing blastomeres of the germline lineage. Coexpressed with par-6; patchy expression observed at the periphery after completion of meiosis I and in meiosis II. On completion of metaphase II, expression is restricted to the anterior 85% of embryo length; this decreases to 55% in embryos between prophase and telophase of the first mitosis. During the first cleavage, expression is detected in the advancing furrow. Transiently coexpressed and colocalized asymmetrically with par-6 and pkc-3, in the developing somatic gonad, including the spermathecal precursor cells of L4 larvae.4 Publications

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Interactioni

Subunit structurei

Required, together with pkc-3, for the localization of par-6; par-6 is involved in localizing/maintaining par-3 at the cell periphery. Interacts with par-6 and pkc-3 for localization at the periphery of anterior cortex of the embryo.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
abu-7O165022EBI-1812329,EBI-328492
CELE_F53A10.2H2L0552EBI-1812329,EBI-2413872
CELE_T11B7.1Q223873EBI-1812329,EBI-320525
pkc-3Q192663EBI-321762,EBI-319158

Protein-protein interaction databases

BioGridi41010. 5 interactions.
DIPiDIP-25317N.
IntActiQ17353. 40 interactions.
MINTiMINT-1103392.

Structurei

3D structure databases

ProteinModelPortaliQ17353.
SMRiQ17353. Positions 69-150, 506-597, 663-758.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini381 – 483103PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini515 – 59985PDZ 2PROSITE-ProRule annotationAdd
BLAST
Domaini659 – 75092PDZ 3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili606 – 62621Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi776 – 81944Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the PAR3 family.1 Publication
Contains 3 PDZ (DHR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG268288.
GeneTreeiENSGT00760000119017.
HOGENOMiHOG000018783.
InParanoidiQ17353.
KOiK04237.
OMAiQANTQYI.
PhylomeDBiQ17353.

Family and domain databases

Gene3Di2.30.42.10. 3 hits.
InterProiIPR021922. DUF3534.
IPR001478. PDZ.
[Graphical view]
PfamiPF12053. DUF3534. 1 hit.
PF00595. PDZ. 3 hits.
[Graphical view]
SMARTiSM00228. PDZ. 3 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 3 hits.
PROSITEiPS50106. PDZ. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform b1 Publication (identifier: Q17353-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSASSTSSSS TSCPEGGEPS GSCKSSDEGE STLKKRMQQY GIASGYANSS
60 70 80 90 100
ISTLDRSQYQ SLPLNGTRRV TVQFGRMKIV VPWKESDQTV GQLADAALLR
110 120 130 140 150
YKKARGMANE DRIHVHRLEC ASDGGILDMD DVLEEVFDLN YDQILAITDE
160 170 180 190 200
ANGGSTTPTY SQIQKQQHHY AQPLPYARKF DGGPSTPIAS AFGSVTVNHQ
210 220 230 240 250
AHRAASPYNV GFARSNSRDF APQPTHSKER RDSVVEVSSF DQIPQSGLRV
260 270 280 290 300
STPKPSRQSE DVIDGKPMNQ PILRSSLRTE ASGSRTEEAT PVKQSRVTLS
310 320 330 340 350
PEVEKKLAEQ DERKSERRKH YDKNPGRFAR GSDRKSRITD ALLDARDRIA
360 370 380 390 400
DQLESQNPAE ETKSQMIRVK IDQGPMPGTS LVTFPPIPEK SENEKQLGIE
410 420 430 440 450
VNAVFDESSE LPGTSEPTKL SSVQIMKIED GGRIAKDGRI RVGDCIVAID
460 470 480 490 500
GKPVDQMSII RVRASISDLA AVTSRPVTLI INRSLESFLE QESSAKPIQS
510 520 530 540 550
ALQQANTQYI GHTTVVELIK SSNGFGFTVT GRETAKGERL FYIGTVKPYG
560 570 580 590 600
VALGHLKSGD RLLEINGTPT GQWTQSEIVE KLKETMVGEK IKFLVSRVSQ
610 620 630 640 650
SAIMSTSASS ENKENEETLK VVEEEKIPQK LPLPALMTPP VPKDTPALSP
660 670 680 690 700
SGASRFEIVI PFINGSSSAG LGVSLKARVS KKSNGSKVDC GIFIKNVMHG
710 720 730 740 750
GAAFKEGGLR VDDRIVGVED IDLEPLDNRE AQAALAKKLK EVGMISSNVR
760 770 780 790 800
LTISRYNECN PGQISRDLSR ITVDASSPSP SSRMSSHTAP DSLLPSPATR
810 820 830 840 850
GTSSSGADSS HSRQSSASSA VPAVPARLTE RDSIVSDGTS RNDESELPDS
860 870 880 890 900
ADPFNREGLG RKSLSEKRGM GAAADPQHIK LFQDIKHQRQ NSAPTSSTQK
910 920 930 940 950
RSKSQPRSSS QRNYRSPMKL VDLPTTAAAS ASTNSQNLDD SDMLNRRSQS
960 970 980 990 1000
MESINRPVES ILRGTGQIPT GSSSKVQFMQ AASPDQHPFP PGAALLRLKN
1010 1020 1030 1040 1050
EESRSRDKSR RKSMGNPFSA MRNFFGFGSK SRDASPEKTP TESVQLRSVE
1060 1070 1080 1090 1100
RPKSIIDERN NGSSERAPPP LPPHQSQRRG SGGNVFVDYG EPYGLIPQYP
1110 1120 1130 1140 1150
HNTTSGYESY ADSELYDRYA AHRYHPRGGP IIDEDEYIYR QQSTSGNSPI
1160 1170 1180 1190 1200
NTSSYVNYGL PASNAYHVGS RIPPQTSSGS ISKTSGAMRR VYPAEYDEDV
1210 1220 1230 1240 1250
AYHQQIPQQS TRYQQGSGSG RGNADYHHMF NSWFAYTGGG AVGAAPVIKS
1260 1270 1280 1290 1300
SYGSSPVRIA AASAIERGES FVVEPVSGSS ASATDRRGRS TSSGAVASGS
1310 1320 1330 1340 1350
SSTGFQYAAK EKYADARSGK FNGGSTRLFI PRHGGGLSAA AFATNFGGEA
1360 1370
YETRGGGAGG SPSQYRRRDQ GPPHRFPQY
Length:1,379
Mass (Da):149,326
Last modified:November 1, 1996 - v1
Checksum:i3B498E7652CFF17D
GO
Isoform a (identifier: Q17353-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1017-1019: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:1,376
Mass (Da):148,994
Checksum:i92CF11545C626856
GO
Isoform c (identifier: Q17353-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-107: MSASSTSSSS...LLRYKKARGM → MHNGRGGRYD...NVTVSKRNFQ
     1017-1019: Missing.

Note: No experimental confirmation available.

Show »
Length:1,340
Mass (Da):145,588
Checksum:i390E4FBB0E9236E0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 107107MSASS…KARGM → MHNGRGGRYDVCPPPPPPPY HFNHVHTPPSKVIVQQQKQQ QKAHREPPPSYPASKMTTTN DNVTVSKRNFQ in isoform c. CuratedVSP_034689Add
BLAST
Alternative sequencei1017 – 10193Missing in isoform a and isoform c. CuratedVSP_051597

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25032 mRNA. Translation: AAB18670.1.
FO080172 Genomic DNA. Translation: CCD61764.1.
FO080172 Genomic DNA. Translation: CCD61765.1.
FO080172 Genomic DNA. Translation: CCD61766.1.
RefSeqiNP_001022607.1. NM_001027436.2. [Q17353-1]
NP_001040857.1. NM_001047392.1. [Q17353-3]
NP_498217.2. NM_065816.6. [Q17353-2]
UniGeneiCel.5328.

Genome annotation databases

EnsemblMetazoaiF54E7.3b.1; F54E7.3b.1; WBGene00003918. [Q17353-1]
F54E7.3b.2; F54E7.3b.2; WBGene00003918. [Q17353-1]
GeneIDi175783.
KEGGicel:CELE_F54E7.3.
UCSCiF54E7.3b. c. elegans. [Q17353-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25032 mRNA. Translation: AAB18670.1 .
FO080172 Genomic DNA. Translation: CCD61764.1 .
FO080172 Genomic DNA. Translation: CCD61765.1 .
FO080172 Genomic DNA. Translation: CCD61766.1 .
RefSeqi NP_001022607.1. NM_001027436.2. [Q17353-1 ]
NP_001040857.1. NM_001047392.1. [Q17353-3 ]
NP_498217.2. NM_065816.6. [Q17353-2 ]
UniGenei Cel.5328.

3D structure databases

ProteinModelPortali Q17353.
SMRi Q17353. Positions 69-150, 506-597, 663-758.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 41010. 5 interactions.
DIPi DIP-25317N.
IntActi Q17353. 40 interactions.
MINTi MINT-1103392.

Proteomic databases

PaxDbi Q17353.
PRIDEi Q17353.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai F54E7.3b.1 ; F54E7.3b.1 ; WBGene00003918 . [Q17353-1 ]
F54E7.3b.2 ; F54E7.3b.2 ; WBGene00003918 . [Q17353-1 ]
GeneIDi 175783.
KEGGi cel:CELE_F54E7.3.
UCSCi F54E7.3b. c. elegans. [Q17353-1 ]

Organism-specific databases

CTDi 175783.
WormBasei F54E7.3a ; CE28449 ; WBGene00003918 ; par-3.
F54E7.3b ; CE28450 ; WBGene00003918 ; par-3.
F54E7.3c ; CE39941 ; WBGene00003918 ; par-3.

Phylogenomic databases

eggNOGi NOG268288.
GeneTreei ENSGT00760000119017.
HOGENOMi HOG000018783.
InParanoidi Q17353.
KOi K04237.
OMAi QANTQYI.
PhylomeDBi Q17353.

Miscellaneous databases

NextBioi 889646.

Family and domain databases

Gene3Di 2.30.42.10. 3 hits.
InterProi IPR021922. DUF3534.
IPR001478. PDZ.
[Graphical view ]
Pfami PF12053. DUF3534. 1 hit.
PF00595. PDZ. 3 hits.
[Graphical view ]
SMARTi SM00228. PDZ. 3 hits.
[Graphical view ]
SUPFAMi SSF50156. SSF50156. 3 hits.
PROSITEi PS50106. PDZ. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Asymmetrically distributed PAR-3 protein contributes to cell polarity and spindle alignment in early C. elegans embryos."
    Etemad-Moghadam B., Guo S., Kemphues K.J.
    Cell 83:743-752(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Bristol N21 Publication.
    Tissue: Embryo1 Publication.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "par-6, a gene involved in the establishment of asymmetry in early C. elegans embryos, mediates the asymmetric localization of PAR-3."
    Watts J.L., Etemad-Moghadam B., Guo S., Boyd L., Draper B.W., Mello C.C., Priess J.R., Kemphues K.J.
    Development 122:3133-3140(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PAR-6.
    Strain: Bristol N21 Publication.
  4. "Atypical protein kinase C cooperates with PAR-3 to establish embryonic polarity in Caenorhabditis elegans."
    Tabuse Y., Izumi Y., Piano F., Kemphues K.J., Miwa J., Ohno S.
    Development 125:3607-3614(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PKC-3.
  5. "PAR-6 is a conserved PDZ domain-containing protein that colocalizes with PAR-3 in Caenorhabditis elegans embryos."
    Hung T.-J., Kemphues K.J.
    Development 126:127-135(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PAR-6 AND PKC-3, TISSUE SPECIFICITY.
    Strain: Bristol N21 Publication.
  6. "C. elegans PAR-3 and PAR-6 are required for apicobasal asymmetries associated with cell adhesion and gastrulation."
    Nance J., Munro E.M., Priess J.R.
    Development 130:5339-5350(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  7. "PAR-3 is required for epithelial cell polarity in the distal spermatheca of C. elegans."
    Aono S., Legouis R., Hoose W.A., Kemphues K.J.
    Development 131:2865-2874(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiPAR3_CAEEL
AccessioniPrimary (citable) accession number: Q17353
Secondary accession number(s): Q27GV0, Q95QE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3