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Protein

Alcohol dehydrogenase class-3

Gene

H24K24.3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.By similarity

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.
S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48Zinc 1; catalyticBy similarity1
Metal bindingi70Zinc 1; catalyticBy similarity1
Metal bindingi100Zinc 2By similarity1
Metal bindingi103Zinc 2By similarity1
Metal bindingi106Zinc 2By similarity1
Metal bindingi114Zinc 2By similarity1
Metal bindingi177Zinc 1; catalyticBy similarity1

GO - Molecular functioni

  • alcohol dehydrogenase activity, zinc-dependent Source: UniProtKB
  • S-(hydroxymethyl)glutathione dehydrogenase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • alcohol metabolic process Source: UniProtKB
  • ethanol oxidation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

ReactomeiR-CEL-2161541. Abacavir metabolism.
R-CEL-5365859. RA biosynthesis pathway.
R-CEL-71384. Ethanol oxidation.

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase class-3 (EC:1.1.1.1)
Alternative name(s):
Alcohol dehydrogenase class-III
Glutathione-dependent formaldehyde dehydrogenase (EC:1.1.1.-)
Short name:
FALDH
Short name:
FDH
Short name:
GSH-FDH
S-(hydroxymethyl)glutathione dehydrogenase (EC:1.1.1.284)
Gene namesi
ORF Names:H24K24.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiH24K24.3a; CE23822; WBGene00019240.
H24K24.3b; CE29978; WBGene00019240.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001607701 – 384Alcohol dehydrogenase class-3Add BLAST384

Proteomic databases

EPDiQ17335.
PaxDbiQ17335.
PeptideAtlasiQ17335.
PRIDEiQ17335.

Expressioni

Gene expression databases

BgeeiWBGene00019240.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi6239.H24K24.3b.1.

Structurei

3D structure databases

ProteinModelPortaliQ17335.
SMRiQ17335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0022. Eukaryota.
COG1062. LUCA.
GeneTreeiENSGT00430000030800.
HOGENOMiHOG000294674.
InParanoidiQ17335.
KOiK00121.
OMAiSIQNYLI.
PhylomeDBiQ17335.

Family and domain databases

CDDicd08300. alcohol_DH_class_III. 1 hit.
Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR014183. ADH_3.
IPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 2 hits.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR02818. adh_III_F_hyde. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q17335-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSTAGQVIN CKAAVAWSAK APLSIETIQV APPKAHEVRV KILYTAVCHT
60 70 80 90 100
DAYTLDGHDP EGLFPVVLGH EGSGIVESVG EGVTGFAPGD HVVPLYVPQC
110 120 130 140 150
KECEYCKNPK TNLCQKIRIS QGNGFMPDGS SRFTCNGKQL FHFMGCSTFS
160 170 180 190 200
EYTVVADISL CKVNPEAPLE KVSLLGCGIS TGYGAVLNTC KVEEGSTVAV
210 220 230 240 250
WGLGAVGLAV IMGAKAAGAK KIVGIDLIES KFESAKFFGA TECINPKSVE
260 270 280 290 300
LPEGKSFQAW LVEQFDGGFD YTFECIGNVH TMRQALEAAH KGWGVSCIIG
310 320 330 340 350
VAGAGQEIAT RPFQLVTGRT WKGTAFGGWK SVESVPRLVD DYMNKKLLID
360 370 380
EFITHRWNID DINTAFDVLH KGESLRSVLA FEKI
Length:384
Mass (Da):41,292
Last modified:November 1, 1996 - v1
Checksum:iE7FA7F7D55CD3908
GO
Isoform b (identifier: Q17335-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     41-41: K → KFQ

Note: No experimental confirmation available.
Show »
Length:386
Mass (Da):41,568
Checksum:iB27E1DF5ADF4EA5D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00020841K → KFQ in isoform b. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18781 mRNA. Translation: AAB03374.1.
FO081563 Genomic DNA. Translation: CCD72435.1.
FO081563 Genomic DNA. Translation: CCD72436.1.
RefSeqiNP_001024016.1. NM_001028845.3. [Q17335-2]
NP_741507.1. NM_171434.5. [Q17335-1]
UniGeneiCel.17999.

Genome annotation databases

EnsemblMetazoaiH24K24.3a.1; H24K24.3a.1; WBGene00019240. [Q17335-1]
H24K24.3a.2; H24K24.3a.2; WBGene00019240. [Q17335-1]
GeneIDi178597.
KEGGicel:CELE_H24K24.3.
UCSCiH24K24.3a.1. c. elegans. [Q17335-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18781 mRNA. Translation: AAB03374.1.
FO081563 Genomic DNA. Translation: CCD72435.1.
FO081563 Genomic DNA. Translation: CCD72436.1.
RefSeqiNP_001024016.1. NM_001028845.3. [Q17335-2]
NP_741507.1. NM_171434.5. [Q17335-1]
UniGeneiCel.17999.

3D structure databases

ProteinModelPortaliQ17335.
SMRiQ17335.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.H24K24.3b.1.

Proteomic databases

EPDiQ17335.
PaxDbiQ17335.
PeptideAtlasiQ17335.
PRIDEiQ17335.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiH24K24.3a.1; H24K24.3a.1; WBGene00019240. [Q17335-1]
H24K24.3a.2; H24K24.3a.2; WBGene00019240. [Q17335-1]
GeneIDi178597.
KEGGicel:CELE_H24K24.3.
UCSCiH24K24.3a.1. c. elegans. [Q17335-1]

Organism-specific databases

CTDi178597.
WormBaseiH24K24.3a; CE23822; WBGene00019240.
H24K24.3b; CE29978; WBGene00019240.

Phylogenomic databases

eggNOGiKOG0022. Eukaryota.
COG1062. LUCA.
GeneTreeiENSGT00430000030800.
HOGENOMiHOG000294674.
InParanoidiQ17335.
KOiK00121.
OMAiSIQNYLI.
PhylomeDBiQ17335.

Enzyme and pathway databases

ReactomeiR-CEL-2161541. Abacavir metabolism.
R-CEL-5365859. RA biosynthesis pathway.
R-CEL-71384. Ethanol oxidation.

Miscellaneous databases

PROiQ17335.

Gene expression databases

BgeeiWBGene00019240.

Family and domain databases

CDDicd08300. alcohol_DH_class_III. 1 hit.
Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR014183. ADH_3.
IPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 2 hits.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR02818. adh_III_F_hyde. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADHX_CAEEL
AccessioniPrimary (citable) accession number: Q17335
Secondary accession number(s): Q8WSP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.