Q17094 (OPSD_ALLSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rhodopsin

Gene names

Name:

RHO

Organism

Alloteuthis subulata (Squid) (Loligo subulata)

Taxonomic identifier

54069 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Cephalopoda › Coleoidea › Neocoleoidea › Decapodiformes › Teuthida › Myopsina › Loliginidae › Alloteuthis

Protein attributes

Sequence length	439 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
Subcellular location	Membrane; Multi-pass membrane protein.
Post-translational modification	Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
Biophysicochemical properties	Absorption: Abs(max)=499 nm

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Chromophore
Molecular function	G-protein coupled receptor Photoreceptor protein Receptor Retinal protein Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological_process	phototransduction Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW photoreceptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›439	›439	Rhodopsin		PRO_0000197733
Regions
	Topological domain	‹1 – 26	›26	Extracellular Potential
	Transmembrane	27 – 51	25	Helical; Name=1; Potential
	Topological domain	52 – 63	12	Cytoplasmic Potential
	Transmembrane	64 – 90	27	Helical; Name=2; Potential
	Topological domain	91 – 104	14	Extracellular Potential
	Transmembrane	105 – 124	20	Helical; Name=3; Potential
	Topological domain	125 – 144	20	Cytoplasmic Potential
	Transmembrane	145 – 168	24	Helical; Name=4; Potential
	Topological domain	169 – 192	24	Extracellular Potential
	Transmembrane	193 – 220	28	Helical; Name=5; Potential
	Topological domain	221 – 254	34	Cytoplasmic Potential
	Transmembrane	255 – 278	24	Helical; Name=6; Potential
	Topological domain	279 – 287	9	Extracellular Potential
	Transmembrane	288 – 311	24	Helical; Name=7; Potential
	Topological domain	312 – ›439	›128	Cytoplasmic Potential
	Compositional bias	363 – 373	11	Met-rich
	Compositional bias	374 – 436	63	Gln/Pro-rich
Amino acid modifications
	Modified residue	298	1	N6-(retinylidene)lysine By similarity
	Lipidation	329	1	S-palmitoyl cysteine By similarity
	Lipidation	330	1	S-palmitoyl cysteine By similarity
	Glycosylation	1	1	N-linked (GlcNAc...) Probable
	Disulfide bond	101 ↔ 179		By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	439	1

Sequences

	Sequence		Length	Mass (Da)
	Q17094 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B8167DFD8A00390E	FASTA	439	49,017
	10 20 30 40 50 60 NETWWYNPYM DIHSHWKQFD QVPAAVYYSL GIFIAICGII GCAGNGIVIY LFTKTKSLQT 70 80 90 100 110 120 PANMFIINLA FSDFTFSLVN GFPMMTISCF LKHWVFGQAA CKVYGLIGGI FGLTSIMTMT 130 140 150 160 170 180 MISIDRYNVI RRPMSASKKM SHRKAFIMIV FVWIWSTIWA IGPIFGWGAY QLEGVLCNCS 190 200 210 220 230 240 FDYITRDAST RSNIVCMYIF AFMFPIVVIF FCYFNIVMSV SNHEKEMAAM AKRLNAKELR 250 260 270 280 290 300 KAQAGASAEM KLAKISIVIV TQSLLSWSPY AIVALLAQFG PIEWVTPYAA QLPVMFAKAS 310 320 330 340 350 360 AIHNPMIYSV SHPKFREAIA SNFPWILTCC QYDEKEIEDD KDAEAEIPAA EQSGGESVDA 370 380 390 400 410 420 AQMKEMMAMM QKMQAQQQQQ PAYPPQGYPP QGYPPPPPQG YPPQGYPPQG YPPQGYPPPP 430 QGPPPQGPPP QAAPPQGVD

References

[1]	"The molecular basis of a spectral shift in the rhodopsins of two species of squid from different photic environments." Morris A., Bowmaker J.K., Hunt D.M. Proc. R. Soc. B 254:233-240(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Epidermis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49108 Genomic DNA. Translation: CAA88923.1.
PIR	S60755.
3D structure databases
ProteinModelPortal	Q17094.
SMR	Q17094. Positions 1-351.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR001760. Opsin. IPR006031. XYPPX. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. PF02162. XYPPX. 3 hits. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00238. OPSIN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. PS00238. OPSIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OPSD_ALLSU

Accession

Primary (citable) accession number: Q17094

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)