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Q17058

- MAL1_APIME

UniProt

Q17058 - MAL1_APIME

Protein

Alpha-glucosidase

Gene
N/A
Organism
Apis mellifera (Honeybee)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Converts sucrose in nectar to glucose and fructose.

    Catalytic activityi

    Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei223 – 2231NucleophileBy similarity
    Active sitei286 – 2861Proton donorBy similarity
    Sitei348 – 3481Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. cation binding Source: InterPro
    2. maltose alpha-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    SABIO-RKQ17058.

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-glucosidase (EC:3.2.1.20)
    Alternative name(s):
    Maltase
    OrganismiApis mellifera (Honeybee)
    Taxonomic identifieri7460 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis
    ProteomesiUP000005203: Unplaced

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 17171 PublicationAdd
    BLAST
    Chaini18 – 567550Alpha-glucosidasePRO_0000001452Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi123 – 1231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi290 – 2901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi313 – 3131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi319 – 3191N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi499 – 4991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi507 – 5071N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ17058.
    PRIDEiQ17058.

    Expressioni

    Tissue specificityi

    Expressed specifically in the hypopharyngeal glands of the forager (worker) honeybee.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi7460.Q17058.

    Structurei

    3D structure databases

    ProteinModelPortaliQ17058.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0366.
    HOGENOMiHOG000220640.
    InParanoidiQ17058.
    OMAiKMLATML.
    PhylomeDBiQ17058.

    Family and domain databases

    Gene3Di3.20.20.80. 2 hits.
    InterProiIPR015902. Glyco_hydro_13.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    [Graphical view]
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q17058-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKAVIVFCLM ALSIVDAAWK PLPENLKEDL IVYQVYPRSF KDSNGDGIGD    50
    IEGIKEKLDH FLEMGVDMFW LSPIYPSPMV DFGYDISNYT DVHPIFGTIS 100
    DLDNLVSAAH EKGLKIILDF VPNHTSDQHE WFQLSLKNIE PYNNYYIWHP 150
    GKIVNGKRVP PTNWVGVFGG SAWSWREERQ AYYLHQFAPE QPDLNYYNPV 200
    VLDDMQNVLR FWLRRGFDGF RVDALPYICE DMRFLDEPLS GETNDPNKTE 250
    YTLKIYTHDI PETYNVVRKF RDVLDEFPQP KHMLIEAYTN LSMTMKYYDY 300
    GADFPFNFAF IKNVSRDSNS SDFKKLVDNW MTYMPPSGIP NWVPGNHDQL 350
    RLVSRFGEEK ARMITTMSLL LPGVAVNYYG DEIGMSDTYI SWEDTQDPQG 400
    CGAGKENYQT MSRDPARTPF QWDDSVSAGF SSSSNTWLRV NENYKTVNLA 450
    AEKKDKNSFF NMFKKFASLK KSPYFKEANL NTRMLNDNVF AFSRETEDNG 500
    SLYAILNFSN EEQIVDLKAF NNVPKKLNMF YNNFNSDIKS ISNNEQVKVS 550
    ALGFFILISQ DAKFGNF 567
    Length:567
    Mass (Da):65,565
    Last modified:November 1, 1996 - v1
    Checksum:iA14D88DD657C99C2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D79208 mRNA. Translation: BAA11466.1.
    PIRiJC4714.
    RefSeqiNP_001011608.1. NM_001011608.1.
    XP_006560868.1. XM_006560805.1.
    XP_006560869.1. XM_006560806.1.
    XP_006560870.1. XM_006560807.1.
    UniGeneiAme.1213.

    Genome annotation databases

    EnsemblMetazoaiGB19017-RA; GB19017-PA; GB19017.
    GeneIDi406131.
    KEGGiame:406131.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D79208 mRNA. Translation: BAA11466.1 .
    PIRi JC4714.
    RefSeqi NP_001011608.1. NM_001011608.1.
    XP_006560868.1. XM_006560805.1.
    XP_006560869.1. XM_006560806.1.
    XP_006560870.1. XM_006560807.1.
    UniGenei Ame.1213.

    3D structure databases

    ProteinModelPortali Q17058.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7460.Q17058.

    Chemistry

    BindingDBi Q17058.
    ChEMBLi CHEMBL4406.

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Proteomic databases

    PaxDbi Q17058.
    PRIDEi Q17058.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai GB19017-RA ; GB19017-PA ; GB19017 .
    GeneIDi 406131.
    KEGGi ame:406131.

    Organism-specific databases

    CTDi 406131.

    Phylogenomic databases

    eggNOGi COG0366.
    HOGENOMi HOG000220640.
    InParanoidi Q17058.
    OMAi KMLATML.
    PhylomeDBi Q17058.

    Enzyme and pathway databases

    SABIO-RK Q17058.

    Family and domain databases

    Gene3Di 3.20.20.80. 2 hits.
    InterProi IPR015902. Glyco_hydro_13.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    [Graphical view ]
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of cDNA and analysis of expression of the gene for alpha-glucosidase from the hypopharyngeal gland of the honeybee apis mellifera L."
      Ohashi K., Sawata M., Takeuchi H., Natori S., Kubo T.
      Biochem. Biophys. Res. Commun. 221:380-385(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-41; 116-137 AND 255-267.
      Tissue: Hypopharyngeal gland.
    2. "Change in the expression of hypopharyngeal-gland proteins of the worker honeybees (Apis mellifera L.) with age and/or role."
      Kubo T., Sasaki M., Nakamura J., Sasagawa H., Ohashi K., Takeuchi H., Natori S.
      J. Biochem. 119:291-295(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Tissue: Hypopharyngeal gland.

    Entry informationi

    Entry nameiMAL1_APIME
    AccessioniPrimary (citable) accession number: Q17058
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3