Q17058 (MAL1_APIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Alpha-glucosidase EC=3.2.1.20 Alternative name(s): Maltase
Organism	Apis mellifera (Honeybee) [Reference proteome]
Taxonomic identifier	7460 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Apoidea › Apidae › Apinae › Apini › Apis

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts sucrose in nectar to glucose and fructose.
Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Subunit structure	Monomer.
Tissue specificity	Expressed specifically in the hypopharyngeal glands of the forager (worker) honeybee.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cation binding Inferred from electronic annotation. Source: InterPro maltose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Ref.1
	Chain	18 – 567	550	Alpha-glucosidase		PRO_0000001452
Sites
	Active site	223	1	Nucleophile By similarity
	Active site	286	1	Proton donor By similarity
	Site	348	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	88	1	N-linked (GlcNAc...) Potential
	Glycosylation	123	1	N-linked (GlcNAc...) Potential
	Glycosylation	247	1	N-linked (GlcNAc...) Potential
	Glycosylation	290	1	N-linked (GlcNAc...) Potential
	Glycosylation	313	1	N-linked (GlcNAc...) Potential
	Glycosylation	319	1	N-linked (GlcNAc...) Potential
	Glycosylation	499	1	N-linked (GlcNAc...) Potential
	Glycosylation	507	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q17058 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A14D88DD657C99C2	FASTA	567	65,565
	10 20 30 40 50 60 MKAVIVFCLM ALSIVDAAWK PLPENLKEDL IVYQVYPRSF KDSNGDGIGD IEGIKEKLDH 70 80 90 100 110 120 FLEMGVDMFW LSPIYPSPMV DFGYDISNYT DVHPIFGTIS DLDNLVSAAH EKGLKIILDF 130 140 150 160 170 180 VPNHTSDQHE WFQLSLKNIE PYNNYYIWHP GKIVNGKRVP PTNWVGVFGG SAWSWREERQ 190 200 210 220 230 240 AYYLHQFAPE QPDLNYYNPV VLDDMQNVLR FWLRRGFDGF RVDALPYICE DMRFLDEPLS 250 260 270 280 290 300 GETNDPNKTE YTLKIYTHDI PETYNVVRKF RDVLDEFPQP KHMLIEAYTN LSMTMKYYDY 310 320 330 340 350 360 GADFPFNFAF IKNVSRDSNS SDFKKLVDNW MTYMPPSGIP NWVPGNHDQL RLVSRFGEEK 370 380 390 400 410 420 ARMITTMSLL LPGVAVNYYG DEIGMSDTYI SWEDTQDPQG CGAGKENYQT MSRDPARTPF 430 440 450 460 470 480 QWDDSVSAGF SSSSNTWLRV NENYKTVNLA AEKKDKNSFF NMFKKFASLK KSPYFKEANL 490 500 510 520 530 540 NTRMLNDNVF AFSRETEDNG SLYAILNFSN EEQIVDLKAF NNVPKKLNMF YNNFNSDIKS 550 560 ISNNEQVKVS ALGFFILISQ DAKFGNF

References

[1]

"Molecular cloning of cDNA and analysis of expression of the gene for alpha-glucosidase from the hypopharyngeal gland of the honeybee apis mellifera L." Ohashi K., Sawata M., Takeuchi H., Natori S., Kubo T. Biochem. Biophys. Res. Commun. 221:380-385(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-41; 116-137 AND 255-267. Tissue: Hypopharyngeal gland.

[2]

"Change in the expression of hypopharyngeal-gland proteins of the worker honeybees (Apis mellifera L.) with age and/or role." Kubo T., Sasaki M., Nakamura J., Sasagawa H., Ohashi K., Takeuchi H., Natori S. J. Biochem. 119:291-295(1996) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Tissue: Hypopharyngeal gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D79208 mRNA. Translation: BAA11466.1.
PIR	JC4714.
RefSeq	NP_001011608.1. NM_001011608.1.
UniGene	Ame.1213.
3D structure databases
ProteinModelPortal	Q17058.
ModBase	Search...
Protein-protein interaction databases
STRING	7460.Q17058.
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Proteomic databases
PaxDb	Q17058.
PRIDE	Q17058.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	GB19017-RA; GB19017-PA; GB19017.
GeneID	406131.
KEGG	ame:406131.
Organism-specific databases
CTD	406131.
Phylogenomic databases
eggNOG	COG0366.
HOGENOM	HOG000220640.
InParanoid	Q17058.
OMA	DVYEMMK.
OrthoDB	EOG48PK1D.
PhylomeDB	Q17058.
Enzyme and pathway databases
SABIO-RK	Q17058.
Family and domain databases
Gene3D	3.20.20.80. 2 hits.
InterPro	IPR015902. Glyco_hydro_13. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...
Other
BindingDB	Q17058.
ChEMBL	CHEMBL4406.

Entry information

Entry name

MAL1_APIME

Accession

Primary (citable) accession number: Q17058

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)