Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-glucosidase

Gene
N/A
Organism
Apis mellifera (Honeybee)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Converts sucrose in nectar to glucose and fructose.

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei223 – 2231NucleophileBy similarity
Active sitei286 – 2861Proton donorBy similarity
Sitei348 – 3481Transition state stabilizerBy similarity

GO - Molecular functioni

  1. alpha-1,4-glucosidase activity Source: UniProtKB-EC
  2. cation binding Source: InterPro
  3. maltose alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKQ17058.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-glucosidase (EC:3.2.1.20)
Alternative name(s):
Maltase
OrganismiApis mellifera (Honeybee)
Taxonomic identifieri7460 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis
ProteomesiUP000005203 Componentsi: Unassembled WGS sequence, Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 17171 PublicationAdd
BLAST
Chaini18 – 567550Alpha-glucosidasePRO_0000001452Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi123 – 1231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi290 – 2901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi313 – 3131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi319 – 3191N-linked (GlcNAc...)Sequence Analysis
Glycosylationi499 – 4991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi507 – 5071N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ17058.
PRIDEiQ17058.

Expressioni

Tissue specificityi

Expressed specifically in the hypopharyngeal glands of the forager (worker) honeybee.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi7460.Q17058.

Structurei

3D structure databases

ProteinModelPortaliQ17058.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0366.
HOGENOMiHOG000220640.
InParanoidiQ17058.
OMAiIFIAEAW.
PhylomeDBiQ17058.

Family and domain databases

Gene3Di3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q17058-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVIVFCLM ALSIVDAAWK PLPENLKEDL IVYQVYPRSF KDSNGDGIGD
60 70 80 90 100
IEGIKEKLDH FLEMGVDMFW LSPIYPSPMV DFGYDISNYT DVHPIFGTIS
110 120 130 140 150
DLDNLVSAAH EKGLKIILDF VPNHTSDQHE WFQLSLKNIE PYNNYYIWHP
160 170 180 190 200
GKIVNGKRVP PTNWVGVFGG SAWSWREERQ AYYLHQFAPE QPDLNYYNPV
210 220 230 240 250
VLDDMQNVLR FWLRRGFDGF RVDALPYICE DMRFLDEPLS GETNDPNKTE
260 270 280 290 300
YTLKIYTHDI PETYNVVRKF RDVLDEFPQP KHMLIEAYTN LSMTMKYYDY
310 320 330 340 350
GADFPFNFAF IKNVSRDSNS SDFKKLVDNW MTYMPPSGIP NWVPGNHDQL
360 370 380 390 400
RLVSRFGEEK ARMITTMSLL LPGVAVNYYG DEIGMSDTYI SWEDTQDPQG
410 420 430 440 450
CGAGKENYQT MSRDPARTPF QWDDSVSAGF SSSSNTWLRV NENYKTVNLA
460 470 480 490 500
AEKKDKNSFF NMFKKFASLK KSPYFKEANL NTRMLNDNVF AFSRETEDNG
510 520 530 540 550
SLYAILNFSN EEQIVDLKAF NNVPKKLNMF YNNFNSDIKS ISNNEQVKVS
560
ALGFFILISQ DAKFGNF
Length:567
Mass (Da):65,565
Last modified:November 1, 1996 - v1
Checksum:iA14D88DD657C99C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D79208 mRNA. Translation: BAA11466.1.
PIRiJC4714.
RefSeqiNP_001011608.1. NM_001011608.1.
XP_006560868.1. XM_006560805.1.
XP_006560869.1. XM_006560806.1.
XP_006560870.1. XM_006560807.1.
UniGeneiAme.1213.

Genome annotation databases

GeneIDi406131.
KEGGiame:406131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D79208 mRNA. Translation: BAA11466.1.
PIRiJC4714.
RefSeqiNP_001011608.1. NM_001011608.1.
XP_006560868.1. XM_006560805.1.
XP_006560869.1. XM_006560806.1.
XP_006560870.1. XM_006560807.1.
UniGeneiAme.1213.

3D structure databases

ProteinModelPortaliQ17058.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7460.Q17058.

Chemistry

BindingDBiQ17058.
ChEMBLiCHEMBL4406.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbiQ17058.
PRIDEiQ17058.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi406131.
KEGGiame:406131.

Organism-specific databases

CTDi406131.

Phylogenomic databases

eggNOGiCOG0366.
HOGENOMiHOG000220640.
InParanoidiQ17058.
OMAiIFIAEAW.
PhylomeDBiQ17058.

Enzyme and pathway databases

SABIO-RKQ17058.

Family and domain databases

Gene3Di3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of cDNA and analysis of expression of the gene for alpha-glucosidase from the hypopharyngeal gland of the honeybee apis mellifera L."
    Ohashi K., Sawata M., Takeuchi H., Natori S., Kubo T.
    Biochem. Biophys. Res. Commun. 221:380-385(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-41; 116-137 AND 255-267.
    Tissue: Hypopharyngeal gland.
  2. "Change in the expression of hypopharyngeal-gland proteins of the worker honeybees (Apis mellifera L.) with age and/or role."
    Kubo T., Sasaki M., Nakamura J., Sasagawa H., Ohashi K., Takeuchi H., Natori S.
    J. Biochem. 119:291-295(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Tissue: Hypopharyngeal gland.

Entry informationi

Entry nameiMAL1_APIME
AccessioniPrimary (citable) accession number: Q17058
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.