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Q17058 (MAL1_APIME) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-glucosidase

EC=3.2.1.20
Alternative name(s):
Maltase
OrganismApis mellifera (Honeybee) [Reference proteome]
Taxonomic identifier7460 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis

Protein attributes

Sequence length567 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Converts sucrose in nectar to glucose and fructose.

Catalytic activity

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Subunit structure

Monomer.

Tissue specificity

Expressed specifically in the hypopharyngeal glands of the forager (worker) honeybee.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Ref.1
Chain18 – 567550Alpha-glucosidase
PRO_0000001452

Sites

Active site2231Nucleophile By similarity
Active site2861Proton donor By similarity
Site3481Transition state stabilizer By similarity

Amino acid modifications

Glycosylation881N-linked (GlcNAc...) Potential
Glycosylation1231N-linked (GlcNAc...) Potential
Glycosylation2471N-linked (GlcNAc...) Potential
Glycosylation2901N-linked (GlcNAc...) Potential
Glycosylation3131N-linked (GlcNAc...) Potential
Glycosylation3191N-linked (GlcNAc...) Potential
Glycosylation4991N-linked (GlcNAc...) Potential
Glycosylation5071N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q17058 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A14D88DD657C99C2

FASTA56765,565
        10         20         30         40         50         60 
MKAVIVFCLM ALSIVDAAWK PLPENLKEDL IVYQVYPRSF KDSNGDGIGD IEGIKEKLDH 

        70         80         90        100        110        120 
FLEMGVDMFW LSPIYPSPMV DFGYDISNYT DVHPIFGTIS DLDNLVSAAH EKGLKIILDF 

       130        140        150        160        170        180 
VPNHTSDQHE WFQLSLKNIE PYNNYYIWHP GKIVNGKRVP PTNWVGVFGG SAWSWREERQ 

       190        200        210        220        230        240 
AYYLHQFAPE QPDLNYYNPV VLDDMQNVLR FWLRRGFDGF RVDALPYICE DMRFLDEPLS 

       250        260        270        280        290        300 
GETNDPNKTE YTLKIYTHDI PETYNVVRKF RDVLDEFPQP KHMLIEAYTN LSMTMKYYDY 

       310        320        330        340        350        360 
GADFPFNFAF IKNVSRDSNS SDFKKLVDNW MTYMPPSGIP NWVPGNHDQL RLVSRFGEEK 

       370        380        390        400        410        420 
ARMITTMSLL LPGVAVNYYG DEIGMSDTYI SWEDTQDPQG CGAGKENYQT MSRDPARTPF 

       430        440        450        460        470        480 
QWDDSVSAGF SSSSNTWLRV NENYKTVNLA AEKKDKNSFF NMFKKFASLK KSPYFKEANL 

       490        500        510        520        530        540 
NTRMLNDNVF AFSRETEDNG SLYAILNFSN EEQIVDLKAF NNVPKKLNMF YNNFNSDIKS 

       550        560 
ISNNEQVKVS ALGFFILISQ DAKFGNF 

« Hide

References

[1]"Molecular cloning of cDNA and analysis of expression of the gene for alpha-glucosidase from the hypopharyngeal gland of the honeybee apis mellifera L."
Ohashi K., Sawata M., Takeuchi H., Natori S., Kubo T.
Biochem. Biophys. Res. Commun. 221:380-385(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-41; 116-137 AND 255-267.
Tissue: Hypopharyngeal gland.
[2]"Change in the expression of hypopharyngeal-gland proteins of the worker honeybees (Apis mellifera L.) with age and/or role."
Kubo T., Sasaki M., Nakamura J., Sasagawa H., Ohashi K., Takeuchi H., Natori S.
J. Biochem. 119:291-295(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Tissue: Hypopharyngeal gland.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D79208 mRNA. Translation: BAA11466.1.
PIRJC4714.
RefSeqNP_001011608.1. NM_001011608.1.
XP_006560868.1. XM_006560805.1.
XP_006560869.1. XM_006560806.1.
XP_006560870.1. XM_006560807.1.
UniGeneAme.1213.

3D structure databases

ProteinModelPortalQ17058.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7460.Q17058.

Chemistry

BindingDBQ17058.
ChEMBLCHEMBL4406.

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbQ17058.
PRIDEQ17058.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaGB19017-RA; GB19017-PA; GB19017.
GeneID406131.
KEGGame:406131.

Organism-specific databases

CTD406131.

Phylogenomic databases

eggNOGCOG0366.
HOGENOMHOG000220640.
InParanoidQ17058.
OMARDVESTN.
PhylomeDBQ17058.

Enzyme and pathway databases

SABIO-RKQ17058.

Family and domain databases

Gene3D3.20.20.80. 2 hits.
InterProIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMAL1_APIME
AccessionPrimary (citable) accession number: Q17058
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: March 19, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries