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Protein

Defensin

Gene

Def1

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the anti Gram-positive activity of immune hemolymph.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Defensin

Keywords - Biological processi

Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Defensin
Gene namesi
Name:Def1
ORF Names:AGAP011294
OrganismiAnopheles gambiae (African malaria mosquito)
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
Proteomesi
  • UP000007062 Componenti: Chromosome 3L

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Propeptidei26 – 6237Sequence analysisPRO_0000006738Add
BLAST
Chaini63 – 10240DefensinPRO_0000006739Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi65 ↔ 92PROSITE-ProRule annotation1 Publication
Disulfide bondi78 ↔ 98PROSITE-ProRule annotation1 Publication
Disulfide bondi82 ↔ 100PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiQ17027.

Expressioni

Developmental stagei

In larvae, transcript is enhanced 4 hours after the bacterial infection, increases up to 12 hours post inoculation and declines by 24 hours and by 30 hours returns to background levels. In adult female, expression is evident by 18 hours after infection. Constitutively expressed in both early and late pupae.1 Publication

Inductioni

By bacterial infection.1 Publication

Structurei

Secondary structure

1
102
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi68 – 736Combined sources
Helixi74 – 763Combined sources
Beta strandi77 – 793Combined sources
Helixi80 – 856Combined sources
Beta strandi90 – 934Combined sources
Beta strandi94 – 963Combined sources
Beta strandi98 – 1003Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E3ENMR-A63-102[»]
2E3FNMR-A63-101[»]
2E3GNMR-A63-102[»]
2NY8NMR-X63-102[»]
2NY9NMR-X63-102[»]
2NZ3NMR-A63-102[»]
ProteinModelPortaliQ17027.
SMRiQ17027. Positions 63-102.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ17027.

Family & Domainsi

Sequence similaritiesi

Belongs to the invertebrate defensin family. Type 1 subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410Z251. LUCA.
InParanoidiQ17027.
OrthoDBiEOG091G1AEF.
PhylomeDBiQ17027.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR001542. Defensin_invertebrate/fungal.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PfamiPF01097. Defensin_2. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS51378. INVERT_DEFENSINS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q17027-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKCATIVCTI AVVLAATLLN GSVQAAPQEE AALSGGANLN TLLDELPEET
60 70 80 90 100
HHAALENYRA KRATCDLASG FGVGSSLCAA HCIARRYRGG YCNSKAVCVC

RN
Length:102
Mass (Da):10,627
Last modified:November 1, 1997 - v1
Checksum:i628834560AEDCD0C
GO

Sequence cautioni

The sequence CAA63775 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti9 – 91T → A in strain: Gbab1064e, Gbab1068c, Gbab5010bs, Gbab5011f, Gbab5024h, Gbbkj16034a, Gbjo4i, Gbjo8h, Gbjo12g, Gbjo15as, Gbng3334as and Gbng3365b.
Natural varianti15 – 151A → S in strain: Gbab1068c, Gbab5011f and Gbab5024h.
Natural varianti18 – 181L → F in strain: Gbbkj16015a, Gbbkj16024b, Gbbkj16025b, Gbbkj16032b and Gbng3347f.
Natural varianti34 – 341S → G in strain: Gbab1068c, Gbab5010bs, Gbab5011f, Gbab5024h, Gbbkj16015a, Gbbkj16024b, Gbbkj16025b, Gbbkj16032b, Gbbkj16034a, Gbjo4i, Gbjo6e, Gbjo7h, Gbjo8h, Gbjo9a, Gbjo12g, Gbjo14c, Gbjo15as, Gbjo22d, Gbng3336g, Gbng3339b, Gbng3340f and Gbng3347f.
Natural varianti37 – 382AN → VS in strain: Gbng3343c.
Natural varianti37 – 371A → G in strain: Gbng3337bs.
Natural varianti37 – 371A → V in strain: Gbab1068c, Gbab5011f, Gbab5024h and Gbng3336g.
Natural varianti51 – 511H → L in strain: Gbjo6e.
Natural varianti69 – 691S → R in strain: Gbng3365b.
Natural varianti75 – 762SS → NN in strain: KWA.
Natural varianti95 – 951K → R in strain: Gbjo3a.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X93562 mRNA. Translation: CAA63775.1. Different initiation.
AF063402 Genomic DNA. Translation: AAC18575.1.
DQ212001 Genomic DNA. Translation: ABB00946.1.
DQ212002 Genomic DNA. Translation: ABB00947.1.
DQ212003 Genomic DNA. Translation: ABB00948.1.
DQ212004 Genomic DNA. Translation: ABB00949.1.
DQ212005 Genomic DNA. Translation: ABB00950.1.
DQ212006 Genomic DNA. Translation: ABB00951.1.
DQ212007 Genomic DNA. Translation: ABB00952.1.
DQ212008 Genomic DNA. Translation: ABB00953.1.
DQ212009 Genomic DNA. Translation: ABB00954.1.
DQ212010 Genomic DNA. Translation: ABB00955.1.
DQ212011 Genomic DNA. Translation: ABB00956.1.
DQ212012 Genomic DNA. Translation: ABB00957.1.
DQ212013 Genomic DNA. Translation: ABB00958.1.
DQ212014 Genomic DNA. Translation: ABB00959.1.
DQ212015 Genomic DNA. Translation: ABB00960.1.
DQ212016 Genomic DNA. Translation: ABB00961.1.
DQ212017 Genomic DNA. Translation: ABB00962.1.
DQ212018 Genomic DNA. Translation: ABB00963.1.
DQ212019 Genomic DNA. Translation: ABB00964.1.
DQ212020 Genomic DNA. Translation: ABB00965.1.
DQ212021 Genomic DNA. Translation: ABB00966.1.
DQ212022 Genomic DNA. Translation: ABB00967.1.
DQ212023 Genomic DNA. Translation: ABB00968.1.
DQ212024 Genomic DNA. Translation: ABB00969.1.
DQ212025 Genomic DNA. Translation: ABB00970.1.
DQ212026 Genomic DNA. Translation: ABB00971.1.
DQ212027 Genomic DNA. Translation: ABB00972.1.
DQ212028 Genomic DNA. Translation: ABB00973.1.
DQ212029 Genomic DNA. Translation: ABB00974.1.
DQ212030 Genomic DNA. Translation: ABB00975.1.
DQ212031 Genomic DNA. Translation: ABB00976.1.
DQ212032 Genomic DNA. Translation: ABB00977.1.
DQ212033 Genomic DNA. Translation: ABB00978.1.
DQ212034 Genomic DNA. Translation: ABB00979.1.
DQ212035 Genomic DNA. Translation: ABB00980.1.
DQ212036 Genomic DNA. Translation: ABB00981.1.
DQ212037 Genomic DNA. Translation: ABB00982.1.
DQ212038 Genomic DNA. Translation: ABB00983.1.
DQ212039 Genomic DNA. Translation: ABB00984.1.
DQ212040 Genomic DNA. Translation: ABB00985.1.
DQ212041 Genomic DNA. Translation: ABB00986.1.
DQ212042 Genomic DNA. Translation: ABB00987.1.
AAAB01008816 Genomic DNA. Translation: EAA05234.4.
RefSeqiXP_309352.4. XM_309352.4.

Genome annotation databases

EnsemblMetazoaiAGAP011294-RA; AGAP011294-PA; AGAP011294.
GeneIDi1270637.
KEGGiaga:AgaP_AGAP011294.
VectorBaseiAGAP011294-RA; AGAP011294-PA; AGAP011294.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X93562 mRNA. Translation: CAA63775.1. Different initiation.
AF063402 Genomic DNA. Translation: AAC18575.1.
DQ212001 Genomic DNA. Translation: ABB00946.1.
DQ212002 Genomic DNA. Translation: ABB00947.1.
DQ212003 Genomic DNA. Translation: ABB00948.1.
DQ212004 Genomic DNA. Translation: ABB00949.1.
DQ212005 Genomic DNA. Translation: ABB00950.1.
DQ212006 Genomic DNA. Translation: ABB00951.1.
DQ212007 Genomic DNA. Translation: ABB00952.1.
DQ212008 Genomic DNA. Translation: ABB00953.1.
DQ212009 Genomic DNA. Translation: ABB00954.1.
DQ212010 Genomic DNA. Translation: ABB00955.1.
DQ212011 Genomic DNA. Translation: ABB00956.1.
DQ212012 Genomic DNA. Translation: ABB00957.1.
DQ212013 Genomic DNA. Translation: ABB00958.1.
DQ212014 Genomic DNA. Translation: ABB00959.1.
DQ212015 Genomic DNA. Translation: ABB00960.1.
DQ212016 Genomic DNA. Translation: ABB00961.1.
DQ212017 Genomic DNA. Translation: ABB00962.1.
DQ212018 Genomic DNA. Translation: ABB00963.1.
DQ212019 Genomic DNA. Translation: ABB00964.1.
DQ212020 Genomic DNA. Translation: ABB00965.1.
DQ212021 Genomic DNA. Translation: ABB00966.1.
DQ212022 Genomic DNA. Translation: ABB00967.1.
DQ212023 Genomic DNA. Translation: ABB00968.1.
DQ212024 Genomic DNA. Translation: ABB00969.1.
DQ212025 Genomic DNA. Translation: ABB00970.1.
DQ212026 Genomic DNA. Translation: ABB00971.1.
DQ212027 Genomic DNA. Translation: ABB00972.1.
DQ212028 Genomic DNA. Translation: ABB00973.1.
DQ212029 Genomic DNA. Translation: ABB00974.1.
DQ212030 Genomic DNA. Translation: ABB00975.1.
DQ212031 Genomic DNA. Translation: ABB00976.1.
DQ212032 Genomic DNA. Translation: ABB00977.1.
DQ212033 Genomic DNA. Translation: ABB00978.1.
DQ212034 Genomic DNA. Translation: ABB00979.1.
DQ212035 Genomic DNA. Translation: ABB00980.1.
DQ212036 Genomic DNA. Translation: ABB00981.1.
DQ212037 Genomic DNA. Translation: ABB00982.1.
DQ212038 Genomic DNA. Translation: ABB00983.1.
DQ212039 Genomic DNA. Translation: ABB00984.1.
DQ212040 Genomic DNA. Translation: ABB00985.1.
DQ212041 Genomic DNA. Translation: ABB00986.1.
DQ212042 Genomic DNA. Translation: ABB00987.1.
AAAB01008816 Genomic DNA. Translation: EAA05234.4.
RefSeqiXP_309352.4. XM_309352.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E3ENMR-A63-102[»]
2E3FNMR-A63-101[»]
2E3GNMR-A63-102[»]
2NY8NMR-X63-102[»]
2NY9NMR-X63-102[»]
2NZ3NMR-A63-102[»]
ProteinModelPortaliQ17027.
SMRiQ17027. Positions 63-102.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ17027.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAGAP011294-RA; AGAP011294-PA; AGAP011294.
GeneIDi1270637.
KEGGiaga:AgaP_AGAP011294.
VectorBaseiAGAP011294-RA; AGAP011294-PA; AGAP011294.

Organism-specific databases

CTDi1270637.

Phylogenomic databases

eggNOGiENOG410Z251. LUCA.
InParanoidiQ17027.
OrthoDBiEOG091G1AEF.
PhylomeDBiQ17027.

Miscellaneous databases

EvolutionaryTraceiQ17027.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR001542. Defensin_invertebrate/fungal.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PfamiPF01097. Defensin_2. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS51378. INVERT_DEFENSINS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEFI_ANOGA
AccessioniPrimary (citable) accession number: Q17027
Secondary accession number(s): O61721
, Q38L95, Q38L99, Q38LA3, Q38LA4, Q38LA6, Q38LB0, Q38LB2, Q38LB3, Q38LC5, Q38LD1, Q38LD3, Q38LD5, Q7QHQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.