Reviewed, UniProtKB/Swiss-Prot Q17025 (CTR2_ANOGA)

Names and origin

Protein names

Recommended name:
    Chymotrypsin-2
    EC=3.4.21.1
Alternative name(s):
    AnChym2

Gene names

Name:	CHYM2
ORF Names:	AGAP006711

Organism

Anopheles gambiae (African malaria mosquito) [Complete proteome]

Taxonomic identifier

7165 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Anophelinae › Anopheles

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.
Subcellular location	Secreted. Ref.1
Tissue specificity	After blood feeding, expression is induced in the midgut epithelium, followed by secretion into the midgut lumen. Ref.1
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 32	15	Activation peptide Potential		PRO_0000027656
	Chain	33 – 258	226	Chymotrypsin-2		PRO_0000027657
Regions
	Domain	33 – 255	223	Peptidase S1
Sites
	Active site	74	1	Charge relay system By similarity
	Active site	119	1	Charge relay system By similarity
	Active site	212	1	Charge relay system By similarity
	Site	206	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	59 ↔ 75		By similarity
	Disulfide bond	182 ↔ 198		By similarity
	Disulfide bond	208 ↔ 232		By similarity
Experimental info
	Sequence conflict	21	1	T → P in CAA79326. Ref.1
	Sequence conflict	21	1	T → P in CAA83567. Ref.1
	Sequence conflict	28	1	H → N in CAA79326. Ref.1
	Sequence conflict	28	1	H → N in CAA83567. Ref.1
	Sequence conflict	37	1	E → V in CAA79326. Ref.1
	Sequence conflict	37	1	E → V in CAA83567. Ref.1
	Sequence conflict	42	1	G → C in CAA79326. Ref.1
	Sequence conflict	42	1	G → C in CAA83567. Ref.1
	Sequence conflict	66	1	N → D in CAA79326. Ref.1
	Sequence conflict	66	1	N → D in CAA83567. Ref.1
	Sequence conflict	166	1	P → R in CAA79326. Ref.1
	Sequence conflict	190	1	K → E in CAA79326. Ref.1
	Sequence conflict	190	1	K → E in CAA83567. Ref.1
	Sequence conflict	194 – 196	3	LGH → FPD in CAA79326. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q17025-1 [UniParc]. Last modified May 16, 2006. Version 3. Checksum: 8C82E91B5C790EB8	FASTA	258	27,928
	10 20 30 40 50 60 MLRKVFAVVS VLLVVSAAKV TKLVLDDHYV NRVVGGEVAK NGSAPYQVSL QVPGWGHNCG 70 80 90 100 110 120 GSLLNNRWVL TAAHCLVGYE PSDLMVLVGT NSLKEGGELL KVDKLLYHSR YNRPQFHNDI 130 140 150 160 170 180 GLMRLEQPVQ FSELVQSVEY LEKAVPVNAT VRLTGWGRTS TNGNVPTLLQ SLNVVTLSNE 190 200 210 220 230 240 DCKAKMGNPK NVDLGHVCTL TKAGEGACNG DSGGPLVYEG KLVGVVNFGV PCGRGFPDGF 250 ARVSYYHEWV RTTMANNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Blood digestion in the malaria mosquito Anopheles gambiae: molecular cloning and biochemical characterization of two inducible chymotrypsins." Vizioli J., Catteruccia F., della Torre A., Reckmann I., Mueller H.M. Eur. J. Biochem. 268:4027-4035(2001) [PubMed: 11453997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Suakoko. Tissue: Midgut.
[2]	"The genome sequence of the malaria mosquito Anopheles gambiae." Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F. , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L. Science 298:129-149(2002) [PubMed: 12364791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PEST.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z18888 mRNA. Translation: CAA79326.1. Z32645 Genomic DNA. Translation: CAA83567.1. AAAB01008807 Genomic DNA. Translation: EAA04684.3.
PIR	S44184.
RefSeq	XP_309032.2.
3D structure databases
HSSP	HSSP built from PDB template 1FDP based on UniProtKB P00746.
ModBase	Search...
Protein family/group databases
MEROPS	S01.166.
Genome annotation databases
Ensembl	AGAP006711. Anopheles gambiae. [Contig view]
GeneID	1270347.
KEGG	aga:AgaP_AGAP006711.
VectorBase	AGAP006711. Anopheles gambiae.
Phylogenomic databases
HOGENOM	Q17025.
Enzyme and pathway databases
BRENDA	3.4.21.1. 165157.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CTR2_ANOGA

Accession

Primary (citable) accession number: Q17025
Secondary accession number(s): Q17026, Q7PT16

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 16, 2006
Last modified:	June 16, 2009
This is version 68 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)