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Q16W22

- LIAS_AEDAE

UniProt

Q16W22 - LIAS_AEDAE

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Protein

Lipoyl synthase, mitochondrial

Gene
AAEL009368
Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi111 – 1111Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi116 – 1161Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi122 – 1221Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi142 – 1421Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi146 – 1461Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi149 – 1491Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
ORF Names:AAEL009368
OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
Taxonomic identifieri7159 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
ProteomesiUP000008820: Unassembled WGS sequence

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 393Lipoyl synthase, mitochondrialUniRule annotationPRO_0000398214
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Interactioni

Protein-protein interaction databases

STRINGi7159.AAEL009368-PA.

Structurei

3D structure databases

ProteinModelPortaliQ16W22.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG7P2XS7.
PhylomeDBiQ16W22.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q16W22-1 [UniParc]FASTAAdd to Basket

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MSQISSNLLR NTVQNGKFGC LKNSIQCKHT AANPLEKIRE RLESGPSFQD    50
FVQNPSYNRD DWTDYEGKLR REKGENDRLR LPPWLKTKIP MGKNFSRIKD 100
QLRELKLATV CEEAKCPNIG ECWGGGEHGT QTATIMLMGD TCTRGCRFCS 150
VKTARVPPPL DPAEPTNTAS AIASWGLDYI VLTSVDRDDL PDGGSNHIAA 200
TIREIKRQNP RIFVECLAPD FRGDLECVKV VAQSGLDVYA HNIETVEALT 250
PFVRDRRARY RQSLDVLRSI KEINPSMITK TSIMLGLGET DEQIEQTMKD 300
LRSVGVDCLT LGQYMQPTKR HLKVIEYVTP EKFKHWETRG NELGFLYTAS 350
GPLVRSSYKA GEFFITSILK NRAEEAERRK EAAGGQDTKT EQT 393
Length:393
Mass (Da):44,306
Last modified:July 25, 2006 - v1
Checksum:i54EF5326151DF5D9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH477577 Genomic DNA. Translation: EAT38774.1.
RefSeqiXP_001659989.1. XM_001659939.1.
UniGeneiAae.14529.

Genome annotation databases

EnsemblMetazoaiAAEL009368-RA; AAEL009368-PA; AAEL009368.
GeneIDi5571879.
KEGGiaag:AaeL_AAEL009368.
VectorBaseiAAEL009368. Aedes aegypti.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH477577 Genomic DNA. Translation: EAT38774.1 .
RefSeqi XP_001659989.1. XM_001659939.1.
UniGenei Aae.14529.

3D structure databases

ProteinModelPortali Q16W22.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7159.AAEL009368-PA.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai AAEL009368-RA ; AAEL009368-PA ; AAEL009368 .
GeneIDi 5571879.
KEGGi aag:AaeL_AAEL009368.
VectorBasei AAEL009368. Aedes aegypti.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG7P2XS7.
PhylomeDBi Q16W22.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Aedes aegypti, a major arbovirus vector."
    Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.
    , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.
    Science 316:1718-1723(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LVPib12.

Entry informationi

Entry nameiLIAS_AEDAE
AccessioniPrimary (citable) accession number: Q16W22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 25, 2006
Last modified: June 11, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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