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Q16MW6 (MTAP_AEDAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
S-methyl-5'-thioadenosine phosphorylase

EC=2.4.2.28
Alternative name(s):
5'-methylthioadenosine phosphorylase
Short name=MTA phosphorylase
Short name=MTAP
Short name=MTAPase
Gene names
ORF Names:AAEL012172, AAEL012179
OrganismAedes aegypti (Yellowfever mosquito) (Culex aegypti) [Reference proteome]
Taxonomic identifier7159 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia

Protein attributes

Sequence length279 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates By similarity. HAMAP-Rule MF_03155

Catalytic activity

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate. HAMAP-Rule MF_03155

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route): step 1/1. HAMAP-Rule MF_03155

Subunit structure

Homotrimer By similarity. HAMAP-Rule MF_03155

Subcellular location

Cytoplasm By similarity. Nucleus By similarity HAMAP-Rule MF_03155.

Sequence similarities

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.

Ontologies

Keywords
   Biological processPurine salvage
   Cellular componentCytoplasm
Nucleus
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-methionine salvage from methylthioadenosine

Inferred from electronic annotation. Source: UniProtKB-UniPathway

purine ribonucleoside salvage

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionS-methyl-5-thioadenosine phosphorylase activity

Inferred from electronic annotation. Source: UniProtKB-EC

phosphorylase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 279279S-methyl-5'-thioadenosine phosphorylase HAMAP-Rule MF_03155
PRO_0000415117

Regions

Region55 – 562Phosphate binding By similarity
Region88 – 892Phosphate binding By similarity
Region215 – 2173Substrate binding By similarity

Sites

Binding site131Phosphate By similarity
Binding site1911Substrate; via amide nitrogen By similarity
Binding site1921Phosphate By similarity
Site1731Important for substrate specificity By similarity
Site2281Important for substrate specificity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q16MW6 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: D4C9933EE90815D2

FASTA27930,396
        10         20         30         40         50         60 
MRPAIKIGII GGSGLDDSQI LESRSEKVVS THFGNPSDVL IEGKIAGVDC VLLARHGRNH 

        70         80         90        100        110        120 
SIMPSNVNYR ANIWALKTVG CTHVIVSTAT GSLQERIHPG DLVIPDNFID RTTKRAQTFY 

       130        140        150        160        170        180 
DGNDMLVGVC HVPMEPAFCS RTREVLIETA KELGLVGVHN KGTVVTIEGP RFSSKAESNL 

       190        200        210        220        230        240 
FRQWGADLVN MTLVPEVVLA KEAGLCYAAI AMATDYDCWR DCGENVNVAD VMATFKKNVT 

       250        260        270 
KVTQLITAVI PKIAEMDWTE TIGELTKTVN GSIMLPHAY 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH477847 Genomic DNA. Translation: EAT35677.1.
CH477847 Genomic DNA. Translation: EAT35678.1.
RefSeqXP_001655912.1. XM_001655862.1.
XP_001655913.1. XM_001655863.1.
UniGeneAae.18816.
Aae.22832.

3D structure databases

ProteinModelPortalQ16MW6.
SMRQ16MW6. Positions 4-276.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7159.AAEL012179-PA.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaAAEL012172-RA; AAEL012172-PA; AAEL012172.
AAEL012179-RA; AAEL012179-PA; AAEL012179.
GeneID5575921.
5575922.
KEGGaag:AaeL_AAEL012172.
aag:AaeL_AAEL012179.
VectorBaseAAEL012172. Aedes aegypti.
AAEL012179. Aedes aegypti.

Phylogenomic databases

eggNOGCOG0005.
HOGENOMHOG000228987.
KOK00772.
OMAMTNHTEA.
OrthoDBEOG771270.
PhylomeDBQ16MW6.

Enzyme and pathway databases

UniPathwayUPA00904; UER00873.

Family and domain databases

Gene3D3.40.50.1580. 1 hit.
HAMAPMF_01963. MTAP.
InterProIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERPTHR11904. PTHR11904. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMSSF53167. SSF53167. 1 hit.
TIGRFAMsTIGR01694. MTAP. 1 hit.
PROSITEPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTAP_AEDAE
AccessionPrimary (citable) accession number: Q16MW6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: July 25, 2006
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways