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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

AAEL012172

Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-methyl-5'-thioadenosine phosphorylase (AAEL012172)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13PhosphateUniRule annotation1
Sitei173Important for substrate specificityUniRule annotation1
Binding sitei191Substrate; via amide nitrogenUniRule annotation1
Binding sitei192PhosphateUniRule annotation1
Sitei228Important for substrate specificityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Short name:
MTAPaseUniRule annotation
Gene namesi
ORF Names:AAEL012172, AAEL012179
OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
Taxonomic identifieri7159 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
Proteomesi
  • UP000008820 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004151171 – 279S-methyl-5'-thioadenosine phosphorylaseAdd BLAST279

Proteomic databases

PRIDEiQ16MW6

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi7159.AAEL012179-PA

Structurei

3D structure databases

ProteinModelPortaliQ16MW6
SMRiQ16MW6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni55 – 56Phosphate bindingUniRule annotation2
Regioni88 – 89Phosphate bindingUniRule annotation2
Regioni215 – 217Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3985 Eukaryota
COG0005 LUCA
HOGENOMiHOG000228987
InParanoidiQ16MW6
KOiK00772
OMAiNLYRSWG
OrthoDBiEOG091G0CQI
PhylomeDBiQ16MW6

Family and domain databases

HAMAPiMF_01963 MTAP, 1 hit
InterProiView protein in InterPro
IPR010044 MTAP
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
IPR018099 Purine_phosphorylase-2_CS
PANTHERiPTHR42679 PTHR42679, 1 hit
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01694 MTAP, 1 hit
PROSITEiView protein in PROSITE
PS01240 PNP_MTAP_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q16MW6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPAIKIGII GGSGLDDSQI LESRSEKVVS THFGNPSDVL IEGKIAGVDC
60 70 80 90 100
VLLARHGRNH SIMPSNVNYR ANIWALKTVG CTHVIVSTAT GSLQERIHPG
110 120 130 140 150
DLVIPDNFID RTTKRAQTFY DGNDMLVGVC HVPMEPAFCS RTREVLIETA
160 170 180 190 200
KELGLVGVHN KGTVVTIEGP RFSSKAESNL FRQWGADLVN MTLVPEVVLA
210 220 230 240 250
KEAGLCYAAI AMATDYDCWR DCGENVNVAD VMATFKKNVT KVTQLITAVI
260 270
PKIAEMDWTE TIGELTKTVN GSIMLPHAY
Length:279
Mass (Da):30,396
Last modified:July 25, 2006 - v1
Checksum:iD4C9933EE90815D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH477847 Genomic DNA Translation: EAT35677.1
CH477847 Genomic DNA Translation: EAT35678.1
RefSeqiXP_001655912.1, XM_001655862.1
XP_001655913.1, XM_001655863.1

Genome annotation databases

EnsemblMetazoaiAAEL012172-RA; AAEL012172-PA; AAEL012172
AAEL012179-RA; AAEL012179-PA; AAEL012179
GeneIDi5575922
KEGGiaag:5575922
VectorBaseiAAEL012172-RB; AAEL012172-PB; AAEL012172
AAEL012172-RC; AAEL012172-PC; AAEL012172

Similar proteinsi

Entry informationi

Entry nameiMTAP_AEDAE
AccessioniPrimary (citable) accession number: Q16MW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: July 25, 2006
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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