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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

AAEL012172

Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131PhosphateUniRule annotation
Sitei173 – 1731Important for substrate specificityUniRule annotation
Binding sitei191 – 1911Substrate; via amide nitrogenUniRule annotation
Binding sitei192 – 1921PhosphateUniRule annotation
Sitei228 – 2281Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. phosphorylase activity Source: InterPro
  2. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Short name:
MTAPaseUniRule annotation
Gene namesi
ORF Names:AAEL012172, AAEL012179
OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
Taxonomic identifieri7159 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
ProteomesiUP000008820 Componenti: Unassembled WGS sequence

Subcellular locationi

Cytoplasm UniRule annotation. Nucleus UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279S-methyl-5'-thioadenosine phosphorylasePRO_0000415117Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi7159.AAEL012179-PA.

Structurei

3D structure databases

ProteinModelPortaliQ16MW6.
SMRiQ16MW6. Positions 4-276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni55 – 562Phosphate bindingUniRule annotation
Regioni88 – 892Phosphate bindingUniRule annotation
Regioni215 – 2173Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228987.
InParanoidiQ16MW6.
KOiK00772.
OMAiMTNHTEA.
OrthoDBiEOG771270.
PhylomeDBiQ16MW6.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q16MW6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPAIKIGII GGSGLDDSQI LESRSEKVVS THFGNPSDVL IEGKIAGVDC
60 70 80 90 100
VLLARHGRNH SIMPSNVNYR ANIWALKTVG CTHVIVSTAT GSLQERIHPG
110 120 130 140 150
DLVIPDNFID RTTKRAQTFY DGNDMLVGVC HVPMEPAFCS RTREVLIETA
160 170 180 190 200
KELGLVGVHN KGTVVTIEGP RFSSKAESNL FRQWGADLVN MTLVPEVVLA
210 220 230 240 250
KEAGLCYAAI AMATDYDCWR DCGENVNVAD VMATFKKNVT KVTQLITAVI
260 270
PKIAEMDWTE TIGELTKTVN GSIMLPHAY
Length:279
Mass (Da):30,396
Last modified:July 25, 2006 - v1
Checksum:iD4C9933EE90815D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH477847 Genomic DNA. Translation: EAT35677.1.
CH477847 Genomic DNA. Translation: EAT35678.1.
RefSeqiXP_001655912.1. XM_001655862.1.
XP_001655913.1. XM_001655863.1.
UniGeneiAae.18816.
Aae.22832.

Genome annotation databases

EnsemblMetazoaiAAEL012172-RA; AAEL012172-PA; AAEL012172.
AAEL012179-RA; AAEL012179-PA; AAEL012179.
GeneIDi5575921.
5575922.
KEGGiaag:AaeL_AAEL012172.
aag:AaeL_AAEL012179.
VectorBaseiAAEL012172. Aedes aegypti.
AAEL012179. Aedes aegypti.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH477847 Genomic DNA. Translation: EAT35677.1.
CH477847 Genomic DNA. Translation: EAT35678.1.
RefSeqiXP_001655912.1. XM_001655862.1.
XP_001655913.1. XM_001655863.1.
UniGeneiAae.18816.
Aae.22832.

3D structure databases

ProteinModelPortaliQ16MW6.
SMRiQ16MW6. Positions 4-276.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7159.AAEL012179-PA.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAAEL012172-RA; AAEL012172-PA; AAEL012172.
AAEL012179-RA; AAEL012179-PA; AAEL012179.
GeneIDi5575921.
5575922.
KEGGiaag:AaeL_AAEL012172.
aag:AaeL_AAEL012179.
VectorBaseiAAEL012172. Aedes aegypti.
AAEL012179. Aedes aegypti.

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228987.
InParanoidiQ16MW6.
KOiK00772.
OMAiMTNHTEA.
OrthoDBiEOG771270.
PhylomeDBiQ16MW6.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Aedes aegypti, a major arbovirus vector."
    Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.
    , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.
    Science 316:1718-1723(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LVPib12.

Entry informationi

Entry nameiMTAP_AEDAE
AccessioniPrimary (citable) accession number: Q16MW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: July 25, 2006
Last modified: January 7, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.