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Q16E06 (SYL_ROSDO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:RD1_0049
OrganismRoseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans) [Complete proteome] [HAMAP]
Taxonomic identifier375451 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRoseobacter

Protein attributes

Sequence length848 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 848848Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009419

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q16E06 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: 50E5E9AC6461A4A7

FASTA84894,820
        10         20         30         40         50         60 
MTRYTPAEIE ARWQKAWREN EVFKAIRSAD KPKYYVLEMF PYPSGRIHMG HVRNYTMGDV 

        70         80         90        100        110        120 
IARYKLATGH NVLHPMGWDA FGMPAENAAM AIGGHPKDWT YGNIKDMRDQ MKPLGLSIDW 

       130        140        150        160        170        180 
SREFATCDPE YYGQQQALFL DFLEAGLVYR KNAIVNWDPV DMTVLANEQV EQGRGWRSGA 

       190        200        210        220        230        240 
LVERRELTQW FFKISDHSEE LLSALDTLEN WPAKVRLMQE NWIGKSRGLQ FAFSTIDGPD 

       250        260        270        280        290        300 
GHDRIEVYTT RPDTLLGASF VGISPDHPLA KLLERDNDNV AAFCAECRKG GTTEEAIETA 

       310        320        330        340        350        360 
EKLGFDTGLR VRHPFDTAAE LPVYIANFIL MDYGTGAIFG CPAHDQRDFE FATKYDLPII 

       370        380        390        400        410        420 
STFLPSQDAD EVLSEAFVPA KTEKVFYNRG FAGAEFQTGL EAIDAAIDFC ESNGIGQGVT 

       430        440        450        460        470        480 
KYRLRDWGLS RQRYWGCPIP VVHCDDCGVV PEKKENLPIE LPYDVSFDTP GNPLNRHPTW 

       490        500        510        520        530        540 
RDTPCPACGK PAQRETDTMD TFVDSSWYFA RFTAPRAETP TVMEDAQYWM NVDQYIGGIE 

       550        560        570        580        590        600 
HAILHLLYSR FFARAMQMTG HLPQSAIEPF DALFTQGMVT HEIYQTRDAN GRPVYHLPED 

       610        620        630        640        650        660 
VTDGRLADGT EVEIIPSAKM SKSKKNVVDP LGIIASYGAD TARWFVLSDS PPERDVEWTA 

       670        680        690        700        710        720 
SGAEAAFKHL TRVWTLSERI GKMDKDAAGQ GDEDLLRAMH VCIHDVTMGI ESFGFNAAIA 

       730        740        750        760        770        780 
KLYAFTAKLQ KSKAGYGAQR TAIMTLAQLM SPMTPHLAED IWAHQGGDGL VTTAPWPRAD 

       790        800        810        820        830        840 
EAMLVSDTVT LPVQINGKRR AEIVISADLS KEEVEKIALA DPAVIRSLNG ATPKKIIVVP 


GRIVNVVV 

« Hide

References

[1]"The complete genome sequence of Roseobacter denitrificans reveals a mixotrophic rather than photosynthetic metabolism."
Swingley W.D., Sadekar S., Mastrian S.D., Matthies H.J., Hao J., Ramos H., Acharya C.R., Conrad A.L., Taylor H.L., Dejesa L.C., Shah M.K., O'Huallachain M.E., Lince M.T., Blankenship R.E., Beatty J.T., Touchman J.W.
J. Bacteriol. 189:683-690(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33942 / OCh 114.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000362 Genomic DNA. Translation: ABG29787.1.
RefSeqYP_680473.1. NC_008209.1.

3D structure databases

ProteinModelPortalQ16E06.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING375451.RD1_0049.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG29787; ABG29787; RD1_0049.
GeneID4198491.
KEGGrde:RD1_0049.
PATRIC23358281. VBIRosDen86677_0046.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAPVIHCPK.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycRDEN375451:GJIZ-45-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ROSDO
AccessionPrimary (citable) accession number: Q16E06
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries