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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei622 – 6221ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRDEN375451:GJIZ-45-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:RD1_0049
OrganismiRoseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans)
Taxonomic identifieri375451 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRoseobacter
ProteomesiUP000007029 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 848848Leucine--tRNA ligasePRO_1000009419Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi375451.RD1_0049.

Structurei

3D structure databases

ProteinModelPortaliQ16E06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5111"HIGH" regionAdd
BLAST
Motifi619 – 6235"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q16E06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRYTPAEIE ARWQKAWREN EVFKAIRSAD KPKYYVLEMF PYPSGRIHMG
60 70 80 90 100
HVRNYTMGDV IARYKLATGH NVLHPMGWDA FGMPAENAAM AIGGHPKDWT
110 120 130 140 150
YGNIKDMRDQ MKPLGLSIDW SREFATCDPE YYGQQQALFL DFLEAGLVYR
160 170 180 190 200
KNAIVNWDPV DMTVLANEQV EQGRGWRSGA LVERRELTQW FFKISDHSEE
210 220 230 240 250
LLSALDTLEN WPAKVRLMQE NWIGKSRGLQ FAFSTIDGPD GHDRIEVYTT
260 270 280 290 300
RPDTLLGASF VGISPDHPLA KLLERDNDNV AAFCAECRKG GTTEEAIETA
310 320 330 340 350
EKLGFDTGLR VRHPFDTAAE LPVYIANFIL MDYGTGAIFG CPAHDQRDFE
360 370 380 390 400
FATKYDLPII STFLPSQDAD EVLSEAFVPA KTEKVFYNRG FAGAEFQTGL
410 420 430 440 450
EAIDAAIDFC ESNGIGQGVT KYRLRDWGLS RQRYWGCPIP VVHCDDCGVV
460 470 480 490 500
PEKKENLPIE LPYDVSFDTP GNPLNRHPTW RDTPCPACGK PAQRETDTMD
510 520 530 540 550
TFVDSSWYFA RFTAPRAETP TVMEDAQYWM NVDQYIGGIE HAILHLLYSR
560 570 580 590 600
FFARAMQMTG HLPQSAIEPF DALFTQGMVT HEIYQTRDAN GRPVYHLPED
610 620 630 640 650
VTDGRLADGT EVEIIPSAKM SKSKKNVVDP LGIIASYGAD TARWFVLSDS
660 670 680 690 700
PPERDVEWTA SGAEAAFKHL TRVWTLSERI GKMDKDAAGQ GDEDLLRAMH
710 720 730 740 750
VCIHDVTMGI ESFGFNAAIA KLYAFTAKLQ KSKAGYGAQR TAIMTLAQLM
760 770 780 790 800
SPMTPHLAED IWAHQGGDGL VTTAPWPRAD EAMLVSDTVT LPVQINGKRR
810 820 830 840
AEIVISADLS KEEVEKIALA DPAVIRSLNG ATPKKIIVVP GRIVNVVV
Length:848
Mass (Da):94,820
Last modified:July 25, 2006 - v1
Checksum:i50E5E9AC6461A4A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000362 Genomic DNA. Translation: ABG29787.1.
RefSeqiWP_011566409.1. NC_008209.1.

Genome annotation databases

EnsemblBacteriaiABG29787; ABG29787; RD1_0049.
KEGGirde:RD1_0049.
PATRICi23358281. VBIRosDen86677_0046.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000362 Genomic DNA. Translation: ABG29787.1.
RefSeqiWP_011566409.1. NC_008209.1.

3D structure databases

ProteinModelPortaliQ16E06.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi375451.RD1_0049.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG29787; ABG29787; RD1_0049.
KEGGirde:RD1_0049.
PATRICi23358281. VBIRosDen86677_0046.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciRDEN375451:GJIZ-45-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33942 / OCh 114.

Entry informationi

Entry nameiSYL_ROSDO
AccessioniPrimary (citable) accession number: Q16E06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: July 22, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.