Reviewed, UniProtKB/Swiss-Prot Q16DV7 (DXS1_ROSDO)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase 1
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase 1
      Short name=DXP synthase 1
      Short name=DXPS 1

Gene names

Name:	dxs1
Ordered Locus Names:	RD1_0101

Organism

Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans) [Complete proteome] [HAMAP]

Taxonomic identifier

375451 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Roseobacter

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity. HAMAP MF_00315
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	1-deoxy-D-xylulose-5-phosphate synthase 1 HAMAP MF_00315		PRO_0000256478

Sequences

	Sequence		Length	Mass (Da)
	Q16DV7-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 40CB2BCB726E55C1	FASTA	645	67,788
	10 20 30 40 50 60 MTDTKTPTLD RVAGPADLRS MSDTELRNVA DELRAEVVEA VSQTGGHLGS SLGVVELSVA 70 80 90 100 110 120 IHAVFDTPRD KLVWDVGHQC YPHKILTGRR KQMPTLRQGG GISGFTKRSE SEYDPFGAAH 130 140 150 160 170 180 SSTSISAALG FTISRDMGQP TGDAVAVIGD GSISAGMAYE AMNNAGHEGR RMFVILNDNE 190 200 210 220 230 240 MSIAPPVGAM SSYLSSLSEL SRAGPFGGLV AIAEGMEQLA PKPVRDGARR ARELVTGLEN 250 260 270 280 290 300 RGTFFEELGF DYIGPIDGHD MAQLLSVLRG AKARATGPVL IHCCTVKGKG YAPAEGSADK 310 320 330 340 350 360 YHGVSKFDVG TGVQAKAKAN APSYTAVFGD ALTTLAEEDP SVVAVTAAMP SGTGVDRMAK 370 380 390 400 410 420 RFPNRVFDVG IAEQHGVTFA AAMAATGLKP FCAIYSTFLQ RGYDQIVHDV ALQGLPVRFA 430 440 450 460 470 480 IDRAGLVGAD GATHAGSFDI GYLGALPGMV VMAAADEAEL VHMVATAGAH NDGPIAFRYP 490 500 510 520 530 540 RGNGTGVPLP ETGRVLEIGK GRMIREGDGE VAILSLGTLL SDCEAAARIL EAEGINATIA 550 560 570 580 590 600 DARFAKPLDM ALIANLVQTH KALITVEQGA MAGFGAMVLQ SMAAEGLLDL GCKVRTMCLP 610 620 630 640 DRFIDQAAPA EMYRDAGLDT MGIVEQVTRV LGRDLKVVTL NAKRR

References

[1]

"The complete genome sequence of Roseobacter denitrificans reveals a mixotrophic rather than photosynthetic metabolism." Swingley W.D., Sadekar S., Mastrian S.D., Matthies H.J., Hao J., Ramos H., Acharya C.R., Conrad A.L., Taylor H.L., Dejesa L.C., Shah M.K., O'Huallachain M.E., Lince M.T., Blankenship R.E., Beatty J.T., Touchman J.W. J. Bacteriol. 189:683-690(2007) [PubMed: 17098896] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000362 Genomic DNA. Translation: ABG29836.1.
RefSeq	YP_680522.1.
3D structure databases
SMR	Q16DV7. Positions 4-631.
ModBase	Search...
Protein-protein interaction databases
STRING	Q16DV7.
Genome annotation databases
GeneID	4198115.
GenomeReviews	Gene locus RD1_0101 in contig CP000362_GR.
KEGG	rde:RD1_0101.
NMPDR	fig|375451.6.peg.95.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	KYPERFW.
PhylomeDB	Q16DV7.
Enzyme and pathway databases
BioCyc	RDEN375451:RD1_0101-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR000399. TPP_bd_CS. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS1_ROSDO

Accession

Primary (citable) accession number: Q16DV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	July 25, 2006
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)