Reviewed, UniProtKB/Swiss-Prot Q16CP0 (DXS2_ROSDO)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase 2
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase 2
      Short name=DXP synthase 2
      Short name=DXPS 2

Gene names

Name:	dxs2
Ordered Locus Names:	RD1_0548

Organism

Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans) [Complete proteome] [HAMAP]

Taxonomic identifier

375451 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Roseobacter

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity.
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 642	642	1-deoxy-D-xylulose-5-phosphate synthase 2 HAMAP MF_00315		PRO_0000256479

Sequences

	Sequence		Length	Mass (Da)
	Q16CP0-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 262F0A60FC7520D7	FASTA	642	68,097
	10 20 30 40 50 60 MTDMPKTPLL DLVHRPADMK GLSDRQLVQL ADELRSETVS AVSVTGGHLG AGLGVVELTV 70 80 90 100 110 120 ALHAVFDTPR DKIIWDVGHQ CYPHKILTER RDRIRTLRMK GGLSGFTKRS ESPYDPFGAA 130 140 150 160 170 180 HSSTSISAAL GFAVARDLGG VVPEGLGDAI AVIGDGSMSA GMAYEAMNNA GHLKKRMIVI 190 200 210 220 230 240 LNDNEMSIAP PTGAMSSYLS RLYSGEPFQD FKAAAKGAVS LLPEPFREGA KRAKDMLKGM 250 260 270 280 290 300 AVGGTLFEEL GFSYIGPIDG HDLDQLLPLL RTVKARATGP IMIHALTKKG KGYAPAETAR 310 320 330 340 350 360 DKGHATAKFD VLTGQQTKAP SNAPSYTKVF AQSLLEEATK DDKICAVTAA MPDGTGLNLF 370 380 390 400 410 420 AERFPSRCFD VGIAEQHGVT FSAALAAGGM KPFCAMYSTF LQRGYDQVVH DVAIQRLPVR 430 440 450 460 470 480 FAIDRAGLVG ADGATHAGSF DIAYLANLPG FVVMAAADEA ELKHMVATAV AHDDGPIAFR 490 500 510 520 530 540 FPRGEGNGVD MPEVGEVLEI GKGRIITEGT RVAILSFGTR LAEVQKAGEA LAARGITPTI 550 560 570 580 590 600 ADARFAKPLD REMILDLVAN HEALITVEEG AIGGFGSHVA QLLSDEGVFD TGFKYRSMVL 610 620 630 640 PDIFIDQSSP ADMYAVAGMN AADIETKVLS VLGIAQIGEA RA

References

[1]

"The complete genome sequence of Roseobacter denitrificans reveals a mixotrophic rather than photosynthetic metabolism." Swingley W.D., Sadekar S., Mastrian S.D., Matthies H.J., Hao J., Ramos H., Acharya C.R., Conrad A.L., Taylor H.L., Dejesa L.C., Shah M.K., O'Huallachain M.E., Lince M.T., Blankenship R.E., Beatty J.T., Touchman J.W. J. Bacteriol. 189:683-690(2007) [PubMed: 17098896] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000362 Genomic DNA. Translation: ABG30253.1.
RefSeq	YP_680939.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q16CP0.
Genome annotation databases
GeneID	4197779.
GenomeReviews	Gene locus RD1_0548 in contig CP000362_GR.
KEGG	rde:RD1_0548.
NMPDR	fig|375451.6.peg.518.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q16CP0.
OMA	QRFPDRY.
Enzyme and pathway databases
BioCyc	RDEN375451:RD1_0548-MON.
Family and domain databases
HAMAP	MF_00315. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS2_ROSDO

Accession

Primary (citable) accession number: Q16CP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	July 25, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)