Q16AX5 (SYE1_ROSDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	RD1_1219

Organism

Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans) [Complete proteome] [HAMAP]

Taxonomic identifier

375451 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Roseobacter

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 441	441	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000367764
Regions
	Motif	8 – 18	11	"HIGH" region HAMAP MF_00022_B
	Motif	239 – 243	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	242	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q16AX5 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 7C0096F3E24FD620	FASTA	441	48,693
	10 20 30 40 50 60 MTTTRFAPSP TGYIHIGNLR TALMNYLIAR KAGGTFILRI DDTDPERSKE EYVDGIKQDL 70 80 90 100 110 120 EWLGLHWDRI ERQSLRLDRY AQAADKLRDM GRFYEAFETP TELDLKRKKQ LNMGKPPVYD 130 140 150 160 170 180 RAALELSDAE KDALRAERGA GVWRFKLERE RIEWADGILG DISIDAASVS DPVLIRGDGQ 190 200 210 220 230 240 ILYTLASVVD DTEMGVTNVV RGSDHVTNTA TQIQIINALG GTVPDFAHHS LLTGPQGEAL 250 260 270 280 290 300 SKRLGTLALR DLRENGVQPA ALLSLMARLG SSDPVELQTD MADLIEGFDI SRFGSAPTKF 310 320 330 340 350 360 DADDLYPLTA RYLAGLPLAD VAGDLSAAGV PDDMAAQFWD VTRENITTLK DIGPWWTLMR 370 380 390 400 410 420 DGAEPQIDDE DREFVAEALA LLPDGPFDAT TWGTWTAAVK EKTGRKGRAL FMPLRKALTG 430 440 QSHGPDMSGL MPLLQVVKAR R

References

[1]

"The complete genome sequence of Roseobacter denitrificans reveals a mixotrophic rather than photosynthetic metabolism." Swingley W.D., Sadekar S., Mastrian S.D., Matthies H.J., Hao J., Ramos H., Acharya C.R., Conrad A.L., Taylor H.L., Dejesa L.C., Shah M.K., O'Huallachain M.E., Lince M.T., Blankenship R.E., Beatty J.T., Touchman J.W. J. Bacteriol. 189:683-690(2007) [PubMed: 17098896] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33942 / OCh 114.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000362 Genomic DNA. Translation: ABG30868.1.
RefSeq	YP_681554.1. NC_008209.1.
3D structure databases
ProteinModelPortal	Q16AX5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q16AX5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4197467.
GenomeReviews	Gene locus RD1_1219 in contig CP000362_GR.
KEGG	rde:RD1_1219.
NMPDR	fig|375451.6.peg.1138.
PATRIC	23360569. VBIRosDen86677_1178.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	ARTALIC.
ProtClustDB	PRK12558.
Enzyme and pathway databases
BioCyc	RDEN375451:RD1_1219-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_ROSDO

Accession

Primary (citable) accession number: Q16AX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 25, 2006
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program