ID   SYK_ROSDO               Reviewed;         527 AA.
AC   Q16AV0;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 1.
DT   03-NOV-2009, entry version 27.
DE   RecName: Full=Lysyl-tRNA synthetase;
DE            EC=6.1.1.6;
DE   AltName: Full=Lysine--tRNA ligase;
DE            Short=LysRS;
GN   Name=lysS; OrderedLocusNames=RD1_1246;
OS   Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter
OS   sp. (strain OCh 114)) (Roseobacter denitrificans).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Roseobacter.
OX   NCBI_TaxID=375451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17098896; DOI=10.1128/JB.01390-06;
RA   Swingley W.D., Sadekar S., Mastrian S.D., Matthies H.J., Hao J.,
RA   Ramos H., Acharya C.R., Conrad A.L., Taylor H.L., Dejesa L.C.,
RA   Shah M.K., O'Huallachain M.E., Lince M.T., Blankenship R.E.,
RA   Beatty J.T., Touchman J.W.;
RT   "The complete genome sequence of Roseobacter denitrificans reveals a
RT   mixotrophic rather than photosynthetic metabolism.";
RL   J. Bacteriol. 189:683-690(2007).
CC   -!- CATALYTIC ACTIVITY: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate
CC       + L-lysyl-tRNA(Lys).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase
CC       family.
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DR   EMBL; CP000362; ABG30893.1; -; Genomic_DNA.
DR   RefSeq; YP_681579.1; -.
DR   STRING; Q16AV0; -.
DR   GeneID; 4197453; -.
DR   GenomeReviews; CP000362_GR; RD1_1246.
DR   KEGG; rde:RD1_1246; -.
DR   NMPDR; fig|375451.6.peg.1163; -.
DR   HOGENOM; Q16AV0; -.
DR   OMA; AFSDDMD; -.
DR   BioCyc; RDEN375451:RD1_1246-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:HAMAP.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:HAMAP.
DR   HAMAP; MF_00177; -; 1.
DR   InterPro; IPR008925; aa-tRNA-synth_I_codon-bd.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002904; Lys-tRNA-synth_Ic_arc-type.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   Gene3D; G3DSA:1.10.10.350; tRNA_synt_bd; 1.
DR   Pfam; PF01921; tRNA-synt_1f; 1.
DR   TIGRFAMs; TIGR00467; lysS_arch; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm;
KW   Ligase; Nucleotide-binding; Protein biosynthesis.
FT   CHAIN         1    527       Lysyl-tRNA synthetase.
FT                                /FTId=PRO_1000040354.
FT   MOTIF        44     52       "HIGH" region.
FT   MOTIF       290    294       "KMSKS" region.
FT   BINDING     293    293       ATP (By similarity).
SQ   SEQUENCE   527 AA;  59384 MW;  23D33C5964D04CA1 CRC64;
     MSDTRTAAMT SKAWPFEEAR RVLKRYEKNP PEKGYVLFET GYGPSGLPHI GTFGEVARTS
     MVMRAFQEIS DIPTRLICFS DDLDGMRKVP GNVPNPDALT EHLQRPLTSV PDPFGTHASF
     GAHNNAMLRR FLDTFGFEYE FISATEFYNT GQFDEILLRA AAKYDEIMAV MLKSLREERR
     QTYSIFLPIH PESGRVMYVP MKEVNAQAGT ITFDSEDGEE MTLPVTGGAV KLQWKPDFGA
     RWAALGVDFE MYGKDHSTNT PIYDKICRIL GQRPPEHFTY ELFLDENGQK ISKSSGNGVS
     IDEWLTYAST ESLSYFMYQK PKTAKRMYFD VIPKAVDEYH QQLRAYAGQD TAQRLNNPVW
     HIHGGDVPAS NMLVPFSMLL NLASVSSAED KSQLWGFIQR YAPESNPENN PDMDAAADFA
     VRYFNDFVKP KKVYRAASDL EREALEDLRD QLKAYDGPVD DEALQSIVYA CGRERFDPLR
     GWFTALYEVL LGASQGPRFG GFIALYGVQE TVALIDAALA GELLSDD
//