Reviewed, UniProtKB/Swiss-Prot Q16AV0 (SYK_ROSDO)

Names and origin

Protein names

Recommended name:
Lysyl-tRNA synthetase
EC=6.1.1.6

Alternative name(s):
Lysine--tRNA ligase
Short name=LysRS

Gene names

Name:	lysS
Ordered Locus Names:	RD1_1246

Organism

Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans) [Complete proteome] [HAMAP]

Taxonomic identifier

375451 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Roseobacter

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP MF_00177
Subcellular location	Cytoplasm HAMAP MF_00177.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 527	527	Lysyl-tRNA synthetase HAMAP MF_00177		PRO_1000040354
Regions
	Motif	44 – 52	9	"HIGH" region HAMAP MF_00177
	Motif	290 – 294	5	"KMSKS" region HAMAP MF_00177
Sites
	Binding site	293	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q16AV0-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 23D33C5964D04CA1	FASTA	527	59,384
	10 20 30 40 50 60 MSDTRTAAMT SKAWPFEEAR RVLKRYEKNP PEKGYVLFET GYGPSGLPHI GTFGEVARTS 70 80 90 100 110 120 MVMRAFQEIS DIPTRLICFS DDLDGMRKVP GNVPNPDALT EHLQRPLTSV PDPFGTHASF 130 140 150 160 170 180 GAHNNAMLRR FLDTFGFEYE FISATEFYNT GQFDEILLRA AAKYDEIMAV MLKSLREERR 190 200 210 220 230 240 QTYSIFLPIH PESGRVMYVP MKEVNAQAGT ITFDSEDGEE MTLPVTGGAV KLQWKPDFGA 250 260 270 280 290 300 RWAALGVDFE MYGKDHSTNT PIYDKICRIL GQRPPEHFTY ELFLDENGQK ISKSSGNGVS 310 320 330 340 350 360 IDEWLTYAST ESLSYFMYQK PKTAKRMYFD VIPKAVDEYH QQLRAYAGQD TAQRLNNPVW 370 380 390 400 410 420 HIHGGDVPAS NMLVPFSMLL NLASVSSAED KSQLWGFIQR YAPESNPENN PDMDAAADFA 430 440 450 460 470 480 VRYFNDFVKP KKVYRAASDL EREALEDLRD QLKAYDGPVD DEALQSIVYA CGRERFDPLR 490 500 510 520 GWFTALYEVL LGASQGPRFG GFIALYGVQE TVALIDAALA GELLSDD

References

[1]

"The complete genome sequence of Roseobacter denitrificans reveals a mixotrophic rather than photosynthetic metabolism." Swingley W.D., Sadekar S., Mastrian S.D., Matthies H.J., Hao J., Ramos H., Acharya C.R., Conrad A.L., Taylor H.L., Dejesa L.C., Shah M.K., O'Huallachain M.E., Lince M.T., Blankenship R.E., Beatty J.T., Touchman J.W. J. Bacteriol. 189:683-690(2007) [PubMed: 17098896] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000362 Genomic DNA. Translation: ABG30893.1.
RefSeq	YP_681579.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q16AV0.
Genome annotation databases
GeneID	4197453.
GenomeReviews	Gene locus RD1_1246 in contig CP000362_GR.
KEGG	rde:RD1_1246.
NMPDR	fig|375451.6.peg.1163.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1384.
HOGENOM	HBG294650.
OMA	AFSDDMD.
ProtClustDB	PRK00750.
Enzyme and pathway databases
BioCyc	RDEN375451:RD1_1246-MONOMER.
Family and domain databases
HAMAP	MF_00177. Lys_tRNA_synth_class1. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR002904. Lys-tRNA-synth_I. IPR020754. Lys-tRNA-synth_Ic. IPR020756. Lysyl-tRNA_synth_I_arc-type. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
Pfam	PF01921. tRNA-synt_1f. 1 hit. [Graphical view]
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00467. lysS_arch. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK_ROSDO

Accession

Primary (citable) accession number: Q16AV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 25, 2006
Last modified:	March 2, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)