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Protein

Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase

Gene

phnN

Organism
Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of ribose 1,5-bisphosphate to 5-phospho-D-ribosyl alpha-1-diphosphate (PRPP).By similarity

Catalytic activityi

ATP + alpha-D-ribose 1,5-bisphosphate = ADP + 5-phospho-alpha-D-ribose 1-diphosphate.
Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphorylase activity Source: InterPro
  3. ribose 1,5-bisphosphate phosphokinase activity Source: UniProtKB-EC
  4. thymidine phosphorylase activity Source: UniProtKB-EC

GO - Biological processi

  1. 5-phosphoribose 1-diphosphate biosynthetic process Source: UniProtKB-UniPathway
  2. organic phosphonate transport Source: UniProtKB-KW
  3. pyrimidine nucleobase metabolic process Source: InterPro
  4. pyrimidine nucleoside metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Alkylphosphonate uptake

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRDEN375451:GJIZ-1440-MONOMER.
UniPathwayiUPA00087; UER00175.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase
Including the following 2 domains:
Ribose 1,5-bisphosphate phosphokinase PhnN (EC:2.7.4.23)
Alternative name(s):
Ribose 1,5-bisphosphokinase
Putative thymidine phosphorylase (EC:2.4.2.4)
Alternative name(s):
TdRPase
Gene namesi
Name:phnN
Ordered Locus Names:RD1_1528
OrganismiRoseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans)
Taxonomic identifieri375451 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRoseobacter
ProteomesiUP000007029 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 677677Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylasePRO_0000314717Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi375451.RD1_1528.

Structurei

3D structure databases

ProteinModelPortaliQ16A32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 187187Ribose 1,5-bisphosphokinaseAdd
BLAST
Regioni188 – 677490ThymidinephosphorylaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the ribose 1,5-bisphosphokinase family.Curated
In the C-terminal section; belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0213.
HOGENOMiHOG000252767.
KOiK00758.
OMAiMNAITRP.
OrthoDBiEOG61ZTGG.

Family and domain databases

Gene3Di3.40.1030.10. 1 hit.
3.40.50.300. 2 hits.
3.90.1170.30. 1 hit.
HAMAPiMF_00703. Thymid_phosp_2.
MF_00836. PhnN.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
IPR008144. Guanylate_kin-like.
IPR027417. P-loop_NTPase.
IPR012699. PhnN.
IPR013102. PYNP_C.
IPR017872. Pyrmidine_PPase_CS.
IPR028579. Thym_Pase_Put.
IPR013466. Thymidine/AMP_Pase.
IPR000053. Thymidine/pyrmidine_PPase.
[Graphical view]
PANTHERiPTHR10515. PTHR10515. 1 hit.
PfamiPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
PF00625. Guanylate_kin. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
SM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMiSSF47648. SSF47648. 1 hit.
SSF52418. SSF52418. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsiTIGR02645. ARCH_P_rylase. 1 hit.
TIGR02322. phosphon_PhnN. 1 hit.
PROSITEiPS50052. GUANYLATE_KINASE_2. 1 hit.
PS00647. THYMID_PHOSPHORYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q16A32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGTLFLIVG PSGVGKDTLL EGARDRLATS RWFSFPQRVV TRAADAGGED
60 70 80 90 100
YIPVTPSEFE QQLAAGAFWH QWHAHGLSYG IPMQVARDLD NGINVVLNAS
110 120 130 140 150
RNEIGAFRDK ATHVVTIGIS APPGIVEERL HERGRESEEE IKRRLARLVE
160 170 180 190 200
QAPLTGYALE IVNDRTIEEG ITALVDLIAG ACDLNAEITR FPVTIGSKQV
210 220 230 240 250
CLVHKGNQIA SRVLAGSSRV TLSLRGKSVS AELGETWSDE IVSQDLCALS
260 270 280 290 300
EGAMAALDAV EGDVVSIERS PTPKSRSILQ KKVRGGELSH AEMEAFIHDL
310 320 330 340 350
VNGRFSTSEI AGFLVAASNN LTMDEVISLT QVRAAFAHRH DWGKAIVVDK
360 370 380 390 400
HSMGGVPGNR ITPIIIPILA AFGLTVPKTS SRAITSAAGT ADMMEVLSRV
410 420 430 440 450
DLSPDEMKSV VEQTNGCIAW NGNLTHSPVD DVMNAINRPL GLQSTLLDVS
460 470 480 490 500
SIMSKKLAAG STHVLIDMPV GPKAKTRTQG EAGALKNLFE SVGQGIGLNT
510 520 530 540 550
KVQISDGTKP IGRGVGPVLE ALDVLSVLRG EAGAPTDLLD KSIGYAATIL
560 570 580 590 600
EWSGAVSQGQ GAQVTRDLVS SGKAFEKLFE IRDAQGRQHD PLQPGQFTED
610 620 630 640 650
ICADRSGRVE AIDIQGISEV ARLSGAPKDK AAGVVLNVQV GDQIAEKQPL
660 670
LRVHSSSLRG IEEAVCAAQA QSAFKIL
Length:677
Mass (Da):72,031
Last modified:July 24, 2006 - v1
Checksum:i21D61E53D52766DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000362 Genomic DNA. Translation: ABG31161.1.
RefSeqiWP_011567781.1. NC_008209.1.
YP_681847.1. NC_008209.1.

Genome annotation databases

EnsemblBacteriaiABG31161; ABG31161; RD1_1528.
GeneIDi4197184.
KEGGirde:RD1_1528.
PATRICi23361171. VBIRosDen86677_1476.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000362 Genomic DNA. Translation: ABG31161.1.
RefSeqiWP_011567781.1. NC_008209.1.
YP_681847.1. NC_008209.1.

3D structure databases

ProteinModelPortaliQ16A32.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi375451.RD1_1528.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG31161; ABG31161; RD1_1528.
GeneIDi4197184.
KEGGirde:RD1_1528.
PATRICi23361171. VBIRosDen86677_1476.

Phylogenomic databases

eggNOGiCOG0213.
HOGENOMiHOG000252767.
KOiK00758.
OMAiMNAITRP.
OrthoDBiEOG61ZTGG.

Enzyme and pathway databases

UniPathwayiUPA00087; UER00175.
BioCyciRDEN375451:GJIZ-1440-MONOMER.

Family and domain databases

Gene3Di3.40.1030.10. 1 hit.
3.40.50.300. 2 hits.
3.90.1170.30. 1 hit.
HAMAPiMF_00703. Thymid_phosp_2.
MF_00836. PhnN.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
IPR008144. Guanylate_kin-like.
IPR027417. P-loop_NTPase.
IPR012699. PhnN.
IPR013102. PYNP_C.
IPR017872. Pyrmidine_PPase_CS.
IPR028579. Thym_Pase_Put.
IPR013466. Thymidine/AMP_Pase.
IPR000053. Thymidine/pyrmidine_PPase.
[Graphical view]
PANTHERiPTHR10515. PTHR10515. 1 hit.
PfamiPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
PF00625. Guanylate_kin. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
SM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMiSSF47648. SSF47648. 1 hit.
SSF52418. SSF52418. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsiTIGR02645. ARCH_P_rylase. 1 hit.
TIGR02322. phosphon_PhnN. 1 hit.
PROSITEiPS50052. GUANYLATE_KINASE_2. 1 hit.
PS00647. THYMID_PHOSPHORYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33942 / OCh 114.

Entry informationi

Entry nameiRBKTP_ROSDO
AccessioniPrimary (citable) accession number: Q16A32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 14, 2008
Last sequence update: July 24, 2006
Last modified: March 31, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.