Q16A32 (RBKTP_ROSDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase

Including the following 2 domains:

1. Ribose 1,5-bisphosphate phosphokinase PhnN
   EC=2.7.4.23
   Alternative name(s):
   Ribose 1,5-bisphosphokinase
2. Putative thymidine phosphorylase
   EC=2.4.2.4
   Alternative name(s):
   TdRPase

Gene names

Name:	phnN
Ordered Locus Names:	RD1_1528

Organism

Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans) [Complete proteome] [HAMAP]

Taxonomic identifier

375451 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Roseobacter › Roseobacter denitrificans

Protein attributes

Sequence length	677 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of ribose 1,5-bisphosphate to 5-phospho-D-ribosyl alpha-1-diphosphate (PRPP) By similarity. HAMAP-Rule MF_00703
Catalytic activity	ATP + alpha-D-ribose 1,5-bisphosphate = ADP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_00703 Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate. HAMAP-Rule MF_00703
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route II): step 3/3. HAMAP-Rule MF_00703
Sequence similarities	In the N-terminal section; belongs to the ribose 1,5-bisphosphokinase family. In the C-terminal section; belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Alkylphosphonate uptake
Ligand	ATP-binding Nucleotide-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	5-phosphoribose 1-diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway organic phosphonate transport Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleobase metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleoside metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorylase activity Inferred from electronic annotation. Source: InterPro ribose 1,5-bisphosphate phosphokinase activity Inferred from electronic annotation. Source: EC thymidine phosphorylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 677	677	Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase HAMAP-Rule MF_00703		PRO_0000314717
Regions
	Region	1 – 187	187	Ribose 1,5-bisphosphokinase HAMAP-Rule MF_00703
	Region	188 – 677	490	Thymidinephosphorylase HAMAP-Rule MF_00703

Sequences

	Sequence		Length	Mass (Da)
	Q16A32 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 21D61E53D52766DE	FASTA	677	72,031
	10 20 30 40 50 60 MPGTLFLIVG PSGVGKDTLL EGARDRLATS RWFSFPQRVV TRAADAGGED YIPVTPSEFE 70 80 90 100 110 120 QQLAAGAFWH QWHAHGLSYG IPMQVARDLD NGINVVLNAS RNEIGAFRDK ATHVVTIGIS 130 140 150 160 170 180 APPGIVEERL HERGRESEEE IKRRLARLVE QAPLTGYALE IVNDRTIEEG ITALVDLIAG 190 200 210 220 230 240 ACDLNAEITR FPVTIGSKQV CLVHKGNQIA SRVLAGSSRV TLSLRGKSVS AELGETWSDE 250 260 270 280 290 300 IVSQDLCALS EGAMAALDAV EGDVVSIERS PTPKSRSILQ KKVRGGELSH AEMEAFIHDL 310 320 330 340 350 360 VNGRFSTSEI AGFLVAASNN LTMDEVISLT QVRAAFAHRH DWGKAIVVDK HSMGGVPGNR 370 380 390 400 410 420 ITPIIIPILA AFGLTVPKTS SRAITSAAGT ADMMEVLSRV DLSPDEMKSV VEQTNGCIAW 430 440 450 460 470 480 NGNLTHSPVD DVMNAINRPL GLQSTLLDVS SIMSKKLAAG STHVLIDMPV GPKAKTRTQG 490 500 510 520 530 540 EAGALKNLFE SVGQGIGLNT KVQISDGTKP IGRGVGPVLE ALDVLSVLRG EAGAPTDLLD 550 560 570 580 590 600 KSIGYAATIL EWSGAVSQGQ GAQVTRDLVS SGKAFEKLFE IRDAQGRQHD PLQPGQFTED 610 620 630 640 650 660 ICADRSGRVE AIDIQGISEV ARLSGAPKDK AAGVVLNVQV GDQIAEKQPL LRVHSSSLRG 670 IEEAVCAAQA QSAFKIL

References

[1]

"The complete genome sequence of Roseobacter denitrificans reveals a mixotrophic rather than photosynthetic metabolism." Swingley W.D., Sadekar S., Mastrian S.D., Matthies H.J., Hao J., Ramos H., Acharya C.R., Conrad A.L., Taylor H.L., Dejesa L.C., Shah M.K., O'Huallachain M.E., Lince M.T., Blankenship R.E., Beatty J.T., Touchman J.W. J. Bacteriol. 189:683-690(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33942 / OCh 114.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000362 Genomic DNA. Translation: ABG31161.1.
RefSeq	YP_681847.1. NC_008209.1.
3D structure databases
ProteinModelPortal	Q16A32.
ModBase	Search...
Protein-protein interaction databases
STRING	375451.RD1_1528.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABG31161; ABG31161; RD1_1528.
GeneID	4197184.
KEGG	rde:RD1_1528.
PATRIC	23361171. VBIRosDen86677_1476.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0213.
HOGENOM	HOG000252767.
KO	K00758.
OMA	FTEDICA.
ProtClustDB	CLSK956389.
Enzyme and pathway databases
BioCyc	RDEN375451:GJIZ-1440-MONOMER.
UniPathway	UPA00087; UER00175.
Family and domain databases
Gene3D	3.40.1030.10. 1 hit. 3.90.1170.30. 1 hit.
HAMAP	MF_00703. Thymid_phosp_2. Fused. MF_00836. PhnN. Fused.
InterPro	IPR000312. Glycosyl_Trfase_fam3. IPR017459. Glycosyl_Trfase_fam3_N_dom. IPR008144. Guanylate_kin. IPR008145. Guanylate_kin/L-typ_Ca_channel. IPR012699. PhnN. IPR013102. PYNP_C. IPR000053. Pyrmidine_PPase. IPR017872. Pyrmidine_PPase_CS. IPR013466. Thymidine_Pase_type2. [Graphical view]
PANTHER	PTHR10515. PTHR10515. 1 hit.
Pfam	PF02885. Glycos_trans_3N. 1 hit. PF00591. Glycos_transf_3. 1 hit. PF00625. Guanylate_kin. 1 hit. PF07831. PYNP_C. 1 hit. [Graphical view]
SMART	SM00072. GuKc. 1 hit. SM00941. PYNP_C. 1 hit. [Graphical view]
SUPFAM	SSF47648. Glyco_trans_3. 1 hit. SSF52418. Glyco_trans_3. 1 hit. SSF54680. PYNP_C. 1 hit.
TIGRFAMs	TIGR02645. ARCH_P_rylase. 1 hit. TIGR02322. phosphon_PhnN. 1 hit.
PROSITE	PS50052. GUANYLATE_KINASE_2. 1 hit. PS00647. THYMID_PHOSPHORYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RBKTP_ROSDO

Accession

Primary (citable) accession number: Q16A32

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 25, 2006
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program