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Protein

Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase

Gene

phnN

Organism
Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of ribose 1,5-bisphosphate to 5-phospho-D-ribosyl alpha-1-diphosphate (PRPP).By similarity

Catalytic activityi

ATP + alpha-D-ribose 1,5-bisphosphate = ADP + 5-phospho-alpha-D-ribose 1-diphosphate.
Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate.

Pathwayi: 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route II).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Phosphopentomutase (deoB)
  2. no protein annotated in this organism
  3. Ribose 1,5-bisphosphate phosphokinase PhnN (phnN), Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase (phnN)
This subpathway is part of the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route II), the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Multifunctional enzyme, Transferase
Biological processAlkylphosphonate uptake
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRDEN375451:G1G6J-1424-MONOMER
UniPathwayiUPA00087; UER00175

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase
Including the following 2 domains:
Ribose 1,5-bisphosphate phosphokinase PhnN (EC:2.7.4.23)
Alternative name(s):
Ribose 1,5-bisphosphokinase
Putative thymidine phosphorylase (EC:2.4.2.4)
Alternative name(s):
TdRPase
Gene namesi
Name:phnN
Ordered Locus Names:RD1_1528
OrganismiRoseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans)
Taxonomic identifieri375451 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRoseobacter
Proteomesi
  • UP000007029 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003147171 – 677Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylaseAdd BLAST677

Interactioni

Protein-protein interaction databases

STRINGi375451.RD1_1528

Structurei

3D structure databases

ProteinModelPortaliQ16A32
SMRiQ16A32
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 187Ribose 1,5-bisphosphokinaseAdd BLAST187
Regioni188 – 677ThymidinephosphorylaseAdd BLAST490

Sequence similaritiesi

In the N-terminal section; belongs to the ribose 1,5-bisphosphokinase family.Curated
In the C-terminal section; belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CMW Bacteria
COG0213 LUCA
COG3709 LUCA
HOGENOMiHOG000252767
KOiK00758
OMAiQMVASIM
OrthoDBiPOG091H02BC

Family and domain databases

Gene3Di3.40.1030.10, 2 hits
3.90.1170.30, 1 hit
HAMAPiMF_00836 PhnN, 1 hit
MF_00703 Thymid_phosp_2, 1 hit
InterProiView protein in InterPro
IPR008145 GK/Ca_channel_bsu
IPR000312 Glycosyl_Trfase_fam3
IPR017459 Glycosyl_Trfase_fam3_N_dom
IPR036320 Glycosyl_Trfase_fam3_N_dom)sf
IPR008144 Guanylate_kin-like_dom
IPR035902 Nuc_phospho_transferase
IPR027417 P-loop_NTPase
IPR012699 PhnN
IPR036566 PYNP-like_C_sf
IPR013102 PYNP_C
IPR017872 Pyrmidine_PPase_CS
IPR028579 Thym_Pase_Put
IPR013466 Thymidine/AMP_Pase
IPR000053 Thymidine/pyrmidine_PPase
PANTHERiPTHR10515 PTHR10515, 1 hit
PfamiView protein in Pfam
PF02885 Glycos_trans_3N, 1 hit
PF00591 Glycos_transf_3, 1 hit
PF00625 Guanylate_kin, 1 hit
PF07831 PYNP_C, 1 hit
SMARTiView protein in SMART
SM00072 GuKc, 1 hit
SM00941 PYNP_C, 1 hit
SUPFAMiSSF47648 SSF47648, 1 hit
SSF52418 SSF52418, 1 hit
SSF52540 SSF52540, 1 hit
SSF54680 SSF54680, 1 hit
TIGRFAMsiTIGR02645 ARCH_P_rylase, 1 hit
TIGR02322 phosphon_PhnN, 1 hit
PROSITEiView protein in PROSITE
PS50052 GUANYLATE_KINASE_2, 1 hit
PS00647 THYMID_PHOSPHORYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q16A32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGTLFLIVG PSGVGKDTLL EGARDRLATS RWFSFPQRVV TRAADAGGED
60 70 80 90 100
YIPVTPSEFE QQLAAGAFWH QWHAHGLSYG IPMQVARDLD NGINVVLNAS
110 120 130 140 150
RNEIGAFRDK ATHVVTIGIS APPGIVEERL HERGRESEEE IKRRLARLVE
160 170 180 190 200
QAPLTGYALE IVNDRTIEEG ITALVDLIAG ACDLNAEITR FPVTIGSKQV
210 220 230 240 250
CLVHKGNQIA SRVLAGSSRV TLSLRGKSVS AELGETWSDE IVSQDLCALS
260 270 280 290 300
EGAMAALDAV EGDVVSIERS PTPKSRSILQ KKVRGGELSH AEMEAFIHDL
310 320 330 340 350
VNGRFSTSEI AGFLVAASNN LTMDEVISLT QVRAAFAHRH DWGKAIVVDK
360 370 380 390 400
HSMGGVPGNR ITPIIIPILA AFGLTVPKTS SRAITSAAGT ADMMEVLSRV
410 420 430 440 450
DLSPDEMKSV VEQTNGCIAW NGNLTHSPVD DVMNAINRPL GLQSTLLDVS
460 470 480 490 500
SIMSKKLAAG STHVLIDMPV GPKAKTRTQG EAGALKNLFE SVGQGIGLNT
510 520 530 540 550
KVQISDGTKP IGRGVGPVLE ALDVLSVLRG EAGAPTDLLD KSIGYAATIL
560 570 580 590 600
EWSGAVSQGQ GAQVTRDLVS SGKAFEKLFE IRDAQGRQHD PLQPGQFTED
610 620 630 640 650
ICADRSGRVE AIDIQGISEV ARLSGAPKDK AAGVVLNVQV GDQIAEKQPL
660 670
LRVHSSSLRG IEEAVCAAQA QSAFKIL
Length:677
Mass (Da):72,031
Last modified:July 25, 2006 - v1
Checksum:i21D61E53D52766DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000362 Genomic DNA Translation: ABG31161.1
RefSeqiWP_011567781.1, NZ_FOOO01000013.1

Genome annotation databases

EnsemblBacteriaiABG31161; ABG31161; RD1_1528
KEGGirde:RD1_1528

Similar proteinsi

Entry informationi

Entry nameiRBKTP_ROSDO
AccessioniPrimary (citable) accession number: Q16A32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: March 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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