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Q16A32 (RBKTP_ROSDO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase

Including the following 2 domains:

  1. Ribose 1,5-bisphosphate phosphokinase PhnN
    EC=2.7.4.23
    Alternative name(s):
    Ribose 1,5-bisphosphokinase
  2. Putative thymidine phosphorylase
    EC=2.4.2.4
    Alternative name(s):
    TdRPase
Gene names
Name:phnN
Ordered Locus Names:RD1_1528
OrganismRoseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans) [Complete proteome] [HAMAP]
Taxonomic identifier375451 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRoseobacter

Protein attributes

Sequence length677 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of ribose 1,5-bisphosphate to 5-phospho-D-ribosyl alpha-1-diphosphate (PRPP) By similarity. HAMAP-Rule MF_00703

Catalytic activity

ATP + alpha-D-ribose 1,5-bisphosphate = ADP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_00703

Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate. HAMAP-Rule MF_00703

Pathway

Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route II): step 3/3. HAMAP-Rule MF_00703

Sequence similarities

In the N-terminal section; belongs to the ribose 1,5-bisphosphokinase family.

In the C-terminal section; belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 677677Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase HAMAP-Rule MF_00703
PRO_0000314717

Regions

Region1 – 187187Ribose 1,5-bisphosphokinase HAMAP-Rule MF_00703
Region188 – 677490Thymidinephosphorylase HAMAP-Rule MF_00703

Sequences

Sequence LengthMass (Da)Tools
Q16A32 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: 21D61E53D52766DE

FASTA67772,031
        10         20         30         40         50         60 
MPGTLFLIVG PSGVGKDTLL EGARDRLATS RWFSFPQRVV TRAADAGGED YIPVTPSEFE 

        70         80         90        100        110        120 
QQLAAGAFWH QWHAHGLSYG IPMQVARDLD NGINVVLNAS RNEIGAFRDK ATHVVTIGIS 

       130        140        150        160        170        180 
APPGIVEERL HERGRESEEE IKRRLARLVE QAPLTGYALE IVNDRTIEEG ITALVDLIAG 

       190        200        210        220        230        240 
ACDLNAEITR FPVTIGSKQV CLVHKGNQIA SRVLAGSSRV TLSLRGKSVS AELGETWSDE 

       250        260        270        280        290        300 
IVSQDLCALS EGAMAALDAV EGDVVSIERS PTPKSRSILQ KKVRGGELSH AEMEAFIHDL 

       310        320        330        340        350        360 
VNGRFSTSEI AGFLVAASNN LTMDEVISLT QVRAAFAHRH DWGKAIVVDK HSMGGVPGNR 

       370        380        390        400        410        420 
ITPIIIPILA AFGLTVPKTS SRAITSAAGT ADMMEVLSRV DLSPDEMKSV VEQTNGCIAW 

       430        440        450        460        470        480 
NGNLTHSPVD DVMNAINRPL GLQSTLLDVS SIMSKKLAAG STHVLIDMPV GPKAKTRTQG 

       490        500        510        520        530        540 
EAGALKNLFE SVGQGIGLNT KVQISDGTKP IGRGVGPVLE ALDVLSVLRG EAGAPTDLLD 

       550        560        570        580        590        600 
KSIGYAATIL EWSGAVSQGQ GAQVTRDLVS SGKAFEKLFE IRDAQGRQHD PLQPGQFTED 

       610        620        630        640        650        660 
ICADRSGRVE AIDIQGISEV ARLSGAPKDK AAGVVLNVQV GDQIAEKQPL LRVHSSSLRG 

       670 
IEEAVCAAQA QSAFKIL 

« Hide

References

[1]"The complete genome sequence of Roseobacter denitrificans reveals a mixotrophic rather than photosynthetic metabolism."
Swingley W.D., Sadekar S., Mastrian S.D., Matthies H.J., Hao J., Ramos H., Acharya C.R., Conrad A.L., Taylor H.L., Dejesa L.C., Shah M.K., O'Huallachain M.E., Lince M.T., Blankenship R.E., Beatty J.T., Touchman J.W.
J. Bacteriol. 189:683-690(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33942 / OCh 114.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000362 Genomic DNA. Translation: ABG31161.1.
RefSeqYP_681847.1. NC_008209.1.

3D structure databases

ProteinModelPortalQ16A32.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING375451.RD1_1528.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG31161; ABG31161; RD1_1528.
GeneID4197184.
KEGGrde:RD1_1528.
PATRIC23361171. VBIRosDen86677_1476.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0213.
HOGENOMHOG000252767.
KOK00758.
OMAMNAITRP.
OrthoDBEOG61ZTGG.
ProtClustDBCLSK956389.

Enzyme and pathway databases

BioCycRDEN375451:GJIZ-1440-MONOMER.
UniPathwayUPA00087; UER00175.

Family and domain databases

Gene3D3.40.1030.10. 1 hit.
3.40.50.300. 2 hits.
3.90.1170.30. 1 hit.
HAMAPMF_00703. Thymid_phosp_2.
MF_00836. PhnN.
InterProIPR008145. GK/Ca_channel_bsu.
IPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
IPR008144. Guanylate_kin-like.
IPR027417. P-loop_NTPase.
IPR012699. PhnN.
IPR013102. PYNP_C.
IPR000053. Pyrmidine_PPase.
IPR017872. Pyrmidine_PPase_CS.
IPR028579. Thym_Pase_Put.
IPR013466. Thymidine/AMP_Pase.
[Graphical view]
PANTHERPTHR10515. PTHR10515. 1 hit.
PfamPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
PF00625. Guanylate_kin. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
SMARTSM00072. GuKc. 1 hit.
SM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMSSF47648. SSF47648. 1 hit.
SSF52418. SSF52418. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsTIGR02645. ARCH_P_rylase. 1 hit.
TIGR02322. phosphon_PhnN. 1 hit.
PROSITEPS50052. GUANYLATE_KINASE_2. 1 hit.
PS00647. THYMID_PHOSPHORYLASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRBKTP_ROSDO
AccessionPrimary (citable) accession number: Q16A32
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways