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Protein

Calcium-independent protein kinase C

Gene

PRKC2

Organism
Aplysia californica (California sea hare)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Requires high levels of phosphatidylserine to be activated. The presence of the C2 domain lowers the affinity of protein kinase C activators for the C1 domains and this inhibition can be removed by phosphatidylserine. Phosphatidic acid, however, is much more potent than phosphatidylserine in reducing C2 domain-mediated inhibition, suggesting that phosphatidic acid may be a required cofactor for the activation of APL II.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei432ATPPROSITE-ProRule annotation1
Active sitei527Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri176 – 226Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri247 – 297Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51
Nucleotide bindingi409 – 417ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.11.13 390

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-independent protein kinase C (EC:2.7.11.13)
Alternative name(s):
APL II
Gene namesi
Name:PRKC2
OrganismiAplysia californica (California sea hare)
Taxonomic identifieri6500 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaHeterobranchiaEuthyneuraEuopisthobranchiaAplysiomorphaAplysioideaAplysiidaeAplysia

Subcellular locationi

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000557281 – 743Calcium-independent protein kinase CAdd BLAST743

PTM databases

iPTMnetiQ16975

Expressioni

Tissue specificityi

Expressed in nervous tissues, ovotestis and gut.

Structurei

3D structure databases

ProteinModelPortaliQ16975
SMRiQ16975
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 108C2PROSITE-ProRule annotationAdd BLAST108
Domaini403 – 663Protein kinasePROSITE-ProRule annotationAdd BLAST261
Domaini665 – 736AGC-kinase C-terminalAdd BLAST72

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri176 – 226Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri247 – 297Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Family and domain databases

CDDicd00029 C1, 2 hits
cd05591 STKc_nPKC_epsilon, 1 hit
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR020454 DAG/PE-bd
IPR011009 Kinase-like_dom_sf
IPR034669 nPKC_epsilon
IPR002219 PE/DAG-bd
IPR017892 Pkinase_C
IPR014376 Prot_kin_PKC_delta
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF00168 C2, 1 hit
PF00069 Pkinase, 1 hit
PF00433 Pkinase_C, 1 hit
PIRSFiPIRSF000551 PKC_delta, 1 hit
PRINTSiPR00008 DAGPEDOMAIN
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00239 C2, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS50004 C2, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits

Sequencei

Sequence statusi: Complete.

Q16975-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRAKMVFN GSVKIKVCEA VDLKPTDFSL RLQKGSTKEK ASQMIEPYVN
60 70 80 90 100
IDVDEVYIAK TTTKPKSVKP QWVWNEDFTS EVHNGQNVNL TVFHDAAIPP
110 120 130 140 150
DEFVANCTIP FDDVKGKSDF WIDLEPNGKL HVVIELCGSA TEAAESTPKE
160 170 180 190 200
KVFKEKEGML NRRRGAMRRR VHQVNGHKFM AMFFRQPTFC SLCRDFIWGL
210 220 230 240 250
GKQGYQCQVC TCVVHKRCHQ HIVTKCPGSR DVANDEVTGK RFNINVPHRF
260 270 280 290 300
NVHNYRRPTF CDHCGSLLYG LVRQGLQCDV CKMNIHKRCQ KNVANNCGTN
310 320 330 340 350
TRDMAQILQE MGISGDKLRP KTKKLSISES HESPNKSSPM HERSLSSPIP
360 370 380 390 400
VIQDSDEAQP GEMGLPSDSL PVNASNEHQG SRTRSPSSDR SRSHHSRISL
410 420 430 440 450
HDFNFIKVLG KGSFGKVMLA EKKGTDEVYA IKVLKKDVII QDDDVECTMT
460 470 480 490 500
EKRILALSAK HPFLTALHSS FQTKERLFFV MEYVNGGDLM FQIQRARKFD
510 520 530 540 550
EPRARFYAAE VTLALMFLHR HGIIYRDLKL DNILLDAEGH CKIADFGMCK
560 570 580 590 600
EGMTENKLTQ TFCGTPDYIA PEILQELKYD ASVDWWALGV LMYEMMAGQP
610 620 630 640 650
PFEADNEDDL FESILHDDVL YPVWLSKEAV SILKGFMTKN PAKRLGCVTT
660 670 680 690 700
QGCEKAILVH PFFHEKIDWE ALEQRKVKPP FKPKIKNKTD ANNFDRDFTS
710 720 730 740
EDPVLTPVDP AVIKTINQEE FRGFSFANPD YGKLEMEASG QAH
Length:743
Mass (Da):84,414
Last modified:November 1, 1996 - v1
Checksum:i4C982C563CA2B659
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94884 mRNA Translation: AAA27771.1
RefSeqiNP_001191401.1, NM_001204472.1
UniGeneiAcl.17890

Genome annotation databases

GeneIDi100533493

Similar proteinsi

Entry informationi

Entry nameiKPC2_APLCA
AccessioniPrimary (citable) accession number: Q16975
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

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