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Protein

Calcium-independent protein kinase C

Gene

PRKC2

Organism
Aplysia californica (California sea hare)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Requires high levels of phosphatidylserine to be activated. The presence of the C2 domain lowers the affinity of protein kinase C activators for the C1 domains and this inhibition can be removed by phosphatidylserine. Phosphatidic acid, however, is much more potent than phosphatidylserine in reducing C2 domain-mediated inhibition, suggesting that phosphatidic acid may be a required cofactor for the activation of APL II.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei432ATPPROSITE-ProRule annotation1
Active sitei527Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri176 – 226Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri247 – 297Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51
Nucleotide bindingi409 – 417ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.11.13. 390.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-independent protein kinase C (EC:2.7.11.13)
Alternative name(s):
APL II
Gene namesi
Name:PRKC2
OrganismiAplysia californica (California sea hare)
Taxonomic identifieri6500 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaHeterobranchiaEuthyneuraEuopisthobranchiaAplysiomorphaAplysioideaAplysiidaeAplysia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000557281 – 743Calcium-independent protein kinase CAdd BLAST743

PTM databases

iPTMnetiQ16975.

Expressioni

Tissue specificityi

Expressed in nervous tissues, ovotestis and gut.

Structurei

3D structure databases

ProteinModelPortaliQ16975.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 108C2PROSITE-ProRule annotationAdd BLAST108
Domaini403 – 663Protein kinasePROSITE-ProRule annotationAdd BLAST261
Domaini665 – 736AGC-kinase C-terminalAdd BLAST72

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri176 – 226Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri247 – 297Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Family and domain databases

CDDicd00029. C1. 2 hits.
Gene3Di2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR017892. Pkinase_C.
IPR014376. Prot_kin_PKC_delta.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000551. PKC_delta. 1 hit.
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q16975-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRAKMVFN GSVKIKVCEA VDLKPTDFSL RLQKGSTKEK ASQMIEPYVN
60 70 80 90 100
IDVDEVYIAK TTTKPKSVKP QWVWNEDFTS EVHNGQNVNL TVFHDAAIPP
110 120 130 140 150
DEFVANCTIP FDDVKGKSDF WIDLEPNGKL HVVIELCGSA TEAAESTPKE
160 170 180 190 200
KVFKEKEGML NRRRGAMRRR VHQVNGHKFM AMFFRQPTFC SLCRDFIWGL
210 220 230 240 250
GKQGYQCQVC TCVVHKRCHQ HIVTKCPGSR DVANDEVTGK RFNINVPHRF
260 270 280 290 300
NVHNYRRPTF CDHCGSLLYG LVRQGLQCDV CKMNIHKRCQ KNVANNCGTN
310 320 330 340 350
TRDMAQILQE MGISGDKLRP KTKKLSISES HESPNKSSPM HERSLSSPIP
360 370 380 390 400
VIQDSDEAQP GEMGLPSDSL PVNASNEHQG SRTRSPSSDR SRSHHSRISL
410 420 430 440 450
HDFNFIKVLG KGSFGKVMLA EKKGTDEVYA IKVLKKDVII QDDDVECTMT
460 470 480 490 500
EKRILALSAK HPFLTALHSS FQTKERLFFV MEYVNGGDLM FQIQRARKFD
510 520 530 540 550
EPRARFYAAE VTLALMFLHR HGIIYRDLKL DNILLDAEGH CKIADFGMCK
560 570 580 590 600
EGMTENKLTQ TFCGTPDYIA PEILQELKYD ASVDWWALGV LMYEMMAGQP
610 620 630 640 650
PFEADNEDDL FESILHDDVL YPVWLSKEAV SILKGFMTKN PAKRLGCVTT
660 670 680 690 700
QGCEKAILVH PFFHEKIDWE ALEQRKVKPP FKPKIKNKTD ANNFDRDFTS
710 720 730 740
EDPVLTPVDP AVIKTINQEE FRGFSFANPD YGKLEMEASG QAH
Length:743
Mass (Da):84,414
Last modified:November 1, 1996 - v1
Checksum:i4C982C563CA2B659
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94884 mRNA. Translation: AAA27771.1.
RefSeqiNP_001191401.1. NM_001204472.1.
UniGeneiAcl.17890.

Genome annotation databases

GeneIDi100533493.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94884 mRNA. Translation: AAA27771.1.
RefSeqiNP_001191401.1. NM_001204472.1.
UniGeneiAcl.17890.

3D structure databases

ProteinModelPortaliQ16975.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiQ16975.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100533493.

Organism-specific databases

CTDi100533493.

Enzyme and pathway databases

BRENDAi2.7.11.13. 390.

Family and domain databases

CDDicd00029. C1. 2 hits.
Gene3Di2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR017892. Pkinase_C.
IPR014376. Prot_kin_PKC_delta.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000551. PKC_delta. 1 hit.
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKPC2_APLCA
AccessioniPrimary (citable) accession number: Q16975
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.