Reviewed, UniProtKB/Swiss-Prot Q16974 (KPC1_APLCA)

Names and origin

Protein names

Recommended name:
    Calcium-dependent protein kinase C
    EC=2.7.11.13
Alternative name(s):
    APL I

Gene names

Name:

PRKC1

Organism

Aplysia californica (California sea hare)

Taxonomic identifier

6500 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Mollusca › Gastropoda › Orthogastropoda › Apogastropoda › Heterobranchia › Euthyneura › Opisthobranchia › Aplysiomorpha › Aplysioidea › Aplysiidae › Aplysia

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is calcium-dependent, phospholipid-dependent, serine- and threonine-specific enzyme. Activation of PKC by serotonin results in presynaptic facilitation of depressed sensory-to-motor neuron synapses, which is thought to underlie behavioral dishabituation. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by phosphatidylserine.
Subcellular location	Cytoplasm. Note: Translocated to neuronal membranes.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase C activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 649	649	Calcium-dependent protein kinase C		PRO_0000055727
Regions
	Domain	157 – 244	88	C2
	Domain	320 – 578	259	Protein kinase
	Domain	579 – 649	71	AGC-kinase C-terminal
	Zinc finger	21 – 71	51	Phorbol-ester/DAG-type 1
	Zinc finger	85 – 135	51	Phorbol-ester/DAG-type 2
	Nucleotide binding	326 – 334	9	ATP By similarity
Sites
	Active site	444	1	Proton acceptor By similarity
	Binding site	349	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q16974-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: A53253399284E330	FASTA	649	74,079
	10 20 30 40 50 60 MEKRVARRGA LRQKNVHEVK NHKFLARFFK QPTFCSHCKD FIWGFGKQGF QCQVCSLVVH 70 80 90 100 110 120 KRCHEFVCFI CPGADKGPDS DATNLHKFKL HSYGSPTFCD HCGSLLYGLL HQGLKCDSCD 130 140 150 160 170 180 MNVHKRCEKN VPLLCGTDHT ERRGRILIKG AVKGSKVLVE ILEAKNLCPM DPNGLADPYV 190 200 210 220 230 240 KVKLIPYDAH KLKLKTKTIK ASLNPVWNES FTVDIGPEDN SKRLSLEVWD WDRTSRNDFM 250 260 270 280 290 300 GSLSFGISEL IKSPVEGWFK LLNQEEGEFY GVPVTDDITE SIQEIKSKMH RSSISSEKRY 310 320 330 340 350 360 PEPDKVQNMS KQDIVRASDF NFLTVLGKGS FGKVVLAERK GTDELYAIKI LKKDVIIQDD 370 380 390 400 410 420 DVECTMIEKR VLALPDKPPF LVQLHSCFQT MDRLYFVMEY VNGGDLMYRI QQEGKFKEPV 430 440 450 460 470 480 AAFYAAEIAI GLFYLHTQGI VYRDLKLDNV MLDAEGHIKI ADFGMCKENI MGDKTTRTFC 490 500 510 520 530 540 GTPDYIAPEI VLYQPYGRSV DWWAYGVLLY EMLAGQPPFD GEDEEELFTS ITDHNVSYPK 550 560 570 580 590 600 ALSREAVSLC KGLLTKTPAK RLGCGPNGER DIKDRAFFRP IQWERIELRE VQPPYKPRIK 610 620 630 640 SRKDVSNFDR EFTSEAPNVT PTDKLFIMNL DQCEFSGFSY VNPEFVVTV

References

[1]	"Cloning and characterization of Ca(2+)-dependent and Ca(2+)-independent PKCs expressed in Aplysia sensory cells." Kruger K.E., Sossin W.S., Sacktor T.C., Bergold P.J., Beushausen S., Schwartz J.H. J. Neurosci. 11:2303-2313(1991) [PubMed: 1869917] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization of two isoforms of protein kinase C in the nervous system of Aplysia californica." Sossin W.S., Diaz-Arrastia R., Schwartz J.H. J. Biol. Chem. 268:5763-5768(1993) [PubMed: 8449941] [Abstract] Cited for: CHARACTERIZATION.
[3]	"The role of C2 domains in Ca2+-activated and Ca2+-independent protein kinase Cs in aplysia." Pepio A.M., Fan X., Sossin W.S. J. Biol. Chem. 273:19040-19048(1998) [PubMed: 9668085] [Abstract] Cited for: CHARACTERIZATION.
[4]	Erratum Pepio A.M., Fan X., Sossin W.S. J. Biol. Chem. 273:22856-22856(1998)

Cross-references

Sequence databases
EMBLGenBankDDBJ	M94883 mRNA. Translation: AAA27770.2.
3D structure databases
HSSP	HSSP built from PDB template 1TBN based on UniProtKB P05697.
SMR	Q16974. Positions 81-143.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.13. 629.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR020477. C2_region. IPR020454. DAG/PE_bd. IPR015745. PKC. IPR017892. Pkinase_C. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR014375. Protein_kinase_C_a/b/g. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22985:SF86. PKC. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF00168. C2. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PIRSF	PIRSF000550. PKC_alpha. 1 hit.
PRINTS	PR00360. C2DOMAIN. PR00008. DAGPEDOMAIN.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 2 hits. SM00239. C2. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50004. C2. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPC1_APLCA

Accession

Primary (citable) accession number: Q16974

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	November 3, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)