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Reviewed, UniProtKB/Swiss-Prot Q16875 (F263_HUMAN)

Last modified July 7, 2009. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Alternative name(s):
    6PF-2-K/Fru-2,6-P2ASE brain/placenta-type isozyme
    iPFK-2
    Renal carcinoma antigen NY-REN-56
Including the following 2 domains:
    1- Recommended name:
            6-phosphofructo-2-kinase
              EC=2.7.1.105
    2- Recommended name:
            Fructose-2,6-bisphosphatase
              EC=3.1.3.46
Gene names
Name: PFKFB3
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Synthesis and degradation of fructose 2,6-bisphosphate.

Catalytic activity

Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.

ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Tissue specificity

Ubiquitous.

Sequence similarities

In the C-terminal section; belongs to the phosphoglycerate mutase family.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-764464,EBI-764464
CUL2Q136171EBI-764464,EBI-456179

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q16875-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q16875-2)

The sequence of this isoform differs from the canonical sequence as follows:
     506-520: NMKGSRSSADSSRKH → PLLGQACLT

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5205206-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
PRO_0000179968

Regions

Nucleotide binding42 – 498ATP By similarity
Region1 – 2452456-phosphofructo-2-kinase
Region246 – 520275Fructose-2,6-bisphosphatase

Sites

Active site1251 Potential
Active site1551 Potential
Active site2541Tele-phosphohistidine intermediate By similarity
Active site3231 Potential
Active site3881Proton donor By similarity
Binding site991Fructose-6-phosphate By similarity
Binding site1901Fructose-6-phosphate By similarity

Amino acid modifications

Modified residue4611Phosphoserine Ref.7
Modified residue4631Phosphothreonine Ref.7
Modified residue4671Phosphoserine Ref.7
Modified residue4711Phosphothreonine; by PKC By similarity

Natural variations

Alternative sequence506 – 52015NMKGS…SSRKH → PLLGQACLT in isoform 2.
VSP_004680

Experimental info

Sequence conflict1361M → V in AAB99795. Ref.3
Sequence conflict1411A → G in AAC62000. Ref.4

Secondary structure

1 ............................................................................ 520
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A7675A4ADC376879

FASTA52059,609
        10         20         30         40         50         60 
MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY ISKKLTRYLN 

        70         80         90        100        110        120 
WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMKVRK QCALAALRDV KSYLAKEGGQ 

       130        140        150        160        170        180 
IAVFDATNTT RERRHMILHF AKENDFKAFF IESVCDDPTV VASNIMEVKI SSPDYKDCNS 

       190        200        210        220        230        240 
AEAMDDFMKR ISCYEASYQP LDPDKCDRDL SLIKVIDVGR RFLVNRVQDH IQSRIVYYLM 

       250        260        270        280        290        300 
NIHVQPRTIY LCRHGENEHN LQGRIGGDSG LSSRGKKFAS ALSKFVEEQN LKDLRVWTSQ 

       310        320        330        340        350        360 
LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ DKYYYRYPTG 

       370        380        390        400        410        420 
ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL DKSAEEMPYL KCPLHTVLKL 

       430        440        450        460        470        480 
TPVAYGCRVE SIYLNVESVC THRERSEDAK KGPNPLMRRN SVTPLASPEP TKKPRINSFE 

       490        500        510        520 
EHVASTSAAL PSCLPPEVPT QLPGQNMKGS RSSADSSRKH

« Hide

Isoform 2.

Checksum: 18FD1D267306853D
Show »

FASTA51458,876

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References

« Hide 'large scale' references
[1]"Cloning of cDNA encoding for a novel isozyme of fructose 6-phosphate, 2-kinase/fructose 2,6-bisphosphatase from human placenta."
Sakai A., Kato M., Fukasawa M., Ishiguro M., Furuya E., Sakakibara R.
J. Biochem. 119:506-511(1996) [PubMed: 8830046] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Placenta.
[2]"Molecular cloning, expression, and chromosomal localization of a ubiquitously expressed human 6-phosphofructo-2-kinase/ fructose-2, 6-bisphosphatase gene (PFKFB3)."
Manzano A., Rosa J.L., Ventura F., Perez J.X., Nadal M., Estivill X., Ambrosio S., Gil J., Bartrons R.
Cytogenet. Cell Genet. 83:214-217(1998) [PubMed: 10072580] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[3]"Human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase."
El-Maghrabi M.R.
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
Tissue: Brain.
[4]"An inducible gene product for 6-phosphofructo-2-kinase with an AU-rich instability element: role in tumor cell glycolysis and the Warburg effect."
Chesney J., Mitchell R.A., Benigni F., Bacher M., Spiegel L., Al-Abed Y., Han J.H., Metz C., Bucala R.
Proc. Natl. Acad. Sci. U.S.A. 96:3047-3052(1999) [PubMed: 10077634] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Skeletal muscle.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[6]"Antigens recognized by autologous antibody in patients with renal-cell carcinoma."
Scanlan M.J., Gordan J.D., Williamson B., Stockert E., Bander N.H., Jongeneel C.V., Gure A.O., Jaeger D., Jaeger E., Knuth A., Chen Y.-T., Old L.J.
Int. J. Cancer 83:456-464(1999) [PubMed: 10508479] [Abstract]
Cited for: IDENTIFICATION AS A RENAL CANCER ANTIGEN.
Tissue: Renal cell carcinoma.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-461; THR-463 AND SER-467, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

D49817 mRNA. Translation: BAA08624.1.
AF109735 mRNA. Translation: AAD08818.1.
AF041831 expand/collapse EMBL AC list , AF041823, AF041824, AF041825, AF041826, AF041827, AF041828, AF041829, AF041830 Genomic DNA. Translation: AAB99795.1.
AF056320 mRNA. Translation: AAC62000.1.
BC040482 mRNA. Translation: AAH40482.1.
IPIIPI00004511.
IPI00216306.
PIRJC4626.
RefSeqNP_001138915.1.
NP_004557.1.
UniGeneHs.195471

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2AXNX-ray2.10A1-520[»]
2DWOX-ray2.25A1-520[»]
2DWPX-ray2.70A1-520[»]
2I1VX-ray2.50B1-520[»]
ModBaseSearch...

Protein-protein interaction databases

IntActQ16875. 3 interactions.

PTM databases

PhosphoSiteQ16875.

Proteomic databases

PRIDEQ16875.

Genome annotation databases

EnsemblENSG00000170525. Homo sapiens. [Contig view]
GeneID5209.
KEGGhsa:5209.
UCSCuc001ije.1. human.

Organism-specific databases

GeneCardsGC10P006226.
H-InvDBHIX0008620.
HGNCHGNC:8874. PFKFB3.
MIM605319. gene.
PharmGKBPA33213.
GenAtlasSearch...

Phylogenomic databases

HOVERGENQ16875.

Enzyme and pathway databases

BRENDA2.7.1.105. 247.
3.1.3.46. 247.
Pathway_Interaction_DBhif1_tfpathway. HIF-1-alpha transcription factor network.
ReactomeREACT_1505. Integration of energy metabolism.
REACT_15380. Diabetes pathways.
REACT_474. Metabolism of carbohydrates.

Gene expression databases

ArrayExpressQ16875.
BgeeQ16875.
CleanExHS_PFKFB3.
GermOnlineENSG00000170525. Homo sapiens.

Family and domain databases

InterProIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR016260. Bifunct_6PFK/fruc_bisP_Ptase.
IPR001345. PG/BPGM_mutase.
IPR013078. PG_mutase.
[Graphical view]
PANTHERPTHR10606. 6Pfruct_kin. 1 hit.
PfamPF01591. 6PF2K. 1 hit.
PF00300. PGAM. 1 hit.
[Graphical view]
PIRSFPIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTSPR00991. 6PFRUCTKNASE.
PROSITEPS00175. PG_MUTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio20146.
SOURCESearch...

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Entry information

Entry nameF263_HUMAN
AccessionPrimary (citable) accession number: Q16875
Secondary accession number(s): O43622, O75902
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: July 7, 2009
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents