Q16864 (VATF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
V-type proton ATPase subunit F
Short name=V-ATPase subunit F

Alternative name(s):
V-ATPase 14 kDa subunit
Vacuolar proton pump subunit F

Gene names

Name:	ATP6V1F
Synonyms:	ATP6S14, VATF

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Subunit structure	V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d).
Sequence similarities	Belongs to the V-ATPase F subunit family.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Coding sequence diversity	Alternative splicing Polymorphism
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP hydrolysis coupled proton transport Inferred from electronic annotation. Source: InterPro cellular iron ion homeostasis Traceable author statement. Source: Reactome insulin receptor signaling pathway Traceable author statement. Source: Reactome interaction with host Traceable author statement. Source: Reactome phagosome maturation Traceable author statement. Source: Reactome transferrin transport Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome proton-transporting V-type ATPase, V1 domain Inferred from electronic annotation. Source: InterPro vacuolar proton-transporting V-type ATPase complex Inferred from direct assay PubMed 18752060. Source: UniProtKB
Molecular_function	ATPase activity, uncoupled Non-traceable author statement Ref.1. Source: UniProtKB hydrogen ion transmembrane transporter activity Non-traceable author statement Ref.1. Source: UniProtKB proton-transporting ATPase activity, rotational mechanism Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q16864-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q16864-2)

The sequence of this isoform differs from the canonical sequence as follows:
     52-52: F → FRSLGSLPGSVVEANPNQRDPPLWDEIDS

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 119	119	V-type proton ATPase subunit F		PRO_0000144799
Natural variations
	Alternative sequence	52	1	F → FRSLGSLPGSVVEANPNQRD PPLWDEIDS in isoform 2.		VSP_045952
	Natural variant	24	1	G → V. Corresponds to variant rs10958 [ dbSNP | Ensembl ].		VAR_048348
Experimental info
	Sequence conflict	8	1	I → T in BAA08392. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 20, 2007. Version 2. Checksum: 9FE5A8BD249A85FA	FASTA	119	13,370
	10 20 30 40 50 60 MAGRGKLIAV IGDEDTVTGF LLGGIGELNK NRHPNFLVVE KDTTINEIED TFRQFLNRDD 70 80 90 100 110 IGIILINQYI AEMVRHALDA HQQSIPAVLE IPSKEHPYDA AKDSILRRAR GMFTAEDLR
	Isoform 2 [UniParc]. Checksum: 964BE12671E7C4DA	FASTA	147	16,402
	10 20 30 40 50 60 MAGRGKLIAV IGDEDTVTGF LLGGIGELNK NRHPNFLVVE KDTTINEIED TFRSLGSLPG 70 80 90 100 110 120 SVVEANPNQR DPPLWDEIDS RQFLNRDDIG IILINQYIAE MVRHALDAHQ QSIPAVLEIP 130 140 SKEHPYDAAK DSILRRARGM FTAEDLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing and expression of a novel cDNA encoding human vacuolar ATPase (14-kDa subunit)." Fujiwara T., Kawai A., Shimizu F., Hirano H., Okuno S., Takeda S., Ozaki K., Shimada Y., Nagata M., Watanabe T., Takaichi A., Nakamura Y., Shin S. DNA Res. 2:107-111(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Fetal brain.
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Lung adenocarcinoma.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D49400 mRNA. Translation: BAA08392.1. CR456896 mRNA. Translation: CAG33177.1. AC025594 Genomic DNA. No translation available. BC104230 mRNA. Translation: AAI04231.1. BC104231 mRNA. Translation: AAI04232.1. BC107854 mRNA. Translation: AAI07855.1. BU956696 mRNA. No translation available.
IPI	IPI00004488. IPI00946714.
PIR	JC4193.
RefSeq	NP_001185838.1. NM_001198909.1. NP_004222.2. NM_004231.3.
UniGene	Hs.78089.
3D structure databases
ProteinModelPortal	Q16864.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q16864. 1 interaction.
MINT	MINT-1426240.
STRING	9606.ENSP00000249289.
Protein family/group databases
TCDB	3.A.2.2.4. H+- or Na+-translocating F-type, V-type and A-type ATPase (F-ATPase) superfamily.
PTM databases
PhosphoSite	Q16864.
Polymorphism databases
DMDM	126302612.
Proteomic databases
PaxDb	Q16864.
PeptideAtlas	Q16864.
PRIDE	Q16864.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000249289; ENSP00000249289; ENSG00000128524. ENST00000492758; ENSP00000417378; ENSG00000128524.
GeneID	9296.
KEGG	hsa:9296.
UCSC	uc003voc.2. human.
Organism-specific databases
CTD	9296.
GeneCards	GC07P128502.
HGNC	HGNC:16832. ATP6V1F.
HPA	CAB009529.
MIM	607160. gene.
neXtProt	NX_Q16864.
PharmGKB	PA38421.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1436.
HOGENOM	HOG000056545.
HOVERGEN	HBG004488.
InParanoid	Q16864.
KO	K02151.
OrthoDB	EOG408N9G.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction. REACT_116125. Disease. REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	Q16864.
Bgee	Q16864.
CleanEx	HS_ATP6V1F.
Genevestigator	Q16864.
GermOnline	ENSG00000128524. Homo sapiens.
Family and domain databases
InterPro	IPR008218. ATPase_V1-cplx_f_g_su. IPR005772. ATPase_V1-cplx_fsu_euk. [Graphical view]
PANTHER	PTHR13861. PTHR13861. 1 hit.
Pfam	PF01990. ATP-synt_F. 1 hit. [Graphical view]
PIRSF	PIRSF015945. ATPase_V1_F_euk. 1 hit.
TIGRFAMs	TIGR01101. V_ATP_synt_F. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	9296.
NextBio	34831.
SOURCE	Search...

Entry information

Entry name

VATF_HUMAN

Accession

Primary (citable) accession number: Q16864
Secondary accession number(s): C9J2K4, Q6IBA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 20, 2007
Last modified:	May 1, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.