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Q16851

- UGPA_HUMAN

UniProt

Q16851 - UGPA_HUMAN

Protein

UTP--glucose-1-phosphate uridylyltransferase

Gene

UGP2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 5 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Plays a central role as a glucosyl donor in cellular metabolic pathways.

    Catalytic activityi

    UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi127 – 1271MagnesiumBy similarity
    Metal bindingi253 – 2531MagnesiumBy similarity
    Active sitei396 – 3961By similarity

    GO - Molecular functioni

    1. glucose binding Source: Ensembl
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: IntAct
    4. pyrimidine ribonucleotide binding Source: Ensembl
    5. UTP:glucose-1-phosphate uridylyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: Reactome
    2. cellular glucuronidation Source: Reactome
    3. glucose 1-phosphate metabolic process Source: Ensembl
    4. glucose metabolic process Source: Reactome
    5. glycogen biosynthetic process Source: Reactome
    6. small molecule metabolic process Source: Reactome
    7. UDP-glucose metabolic process Source: ProtInc
    8. UDP-glucuronate biosynthetic process Source: Reactome
    9. xenobiotic metabolic process Source: Reactome

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS10006-MONOMER.
    ReactomeiREACT_169208. Glycogen synthesis.
    REACT_6737. Formation of the active cofactor, UDP-glucuronate.
    SABIO-RKQ16851.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UTP--glucose-1-phosphate uridylyltransferase (EC:2.7.7.9)
    Alternative name(s):
    UDP-glucose pyrophosphorylase
    Short name:
    UDPGP
    Short name:
    UGPase
    Gene namesi
    Name:UGP2
    Synonyms:UGP1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:12527. UGP2.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. extracellular vesicular exosome Source: UniProt
    3. nucleus Source: UniProt

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi123 – 1231C → S: No significant loss of activity.
    Mutagenesisi218 – 2181W → S: No significant loss of activity.
    Mutagenesisi266 – 2661H → R: No significant loss of activity.
    Mutagenesisi333 – 3331W → S: Loss of activity; possibly due to folding defect.
    Mutagenesisi389 – 3891R → H: No significant loss of activity.
    Mutagenesisi391 – 3911R → H: Loss of activity; possibly due to folding defect.
    Mutagenesisi422 – 4221R → H: No significant loss of activity.
    Mutagenesisi445 – 4451R → H: No significant loss of activity.
    Mutagenesisi502 – 5032NL → PE: Abolishes oligomerization and significantly increases enzymatic activity.

    Organism-specific databases

    PharmGKBiPA37172.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 508508UTP--glucose-1-phosphate uridylyltransferasePRO_0000185752Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei13 – 131Phosphoserine2 Publications
    Modified residuei438 – 4381N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ16851.
    PaxDbiQ16851.
    PRIDEiQ16851.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00395676.
    UCD-2DPAGEQ16851.

    PTM databases

    PhosphoSiteiQ16851.

    Expressioni

    Gene expression databases

    ArrayExpressiQ16851.
    BgeeiQ16851.
    CleanExiHS_UGP2.
    GenevestigatoriQ16851.

    Organism-specific databases

    HPAiHPA034696.

    Interactioni

    Subunit structurei

    Homooctamer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GRB2P629932EBI-743729,EBI-401755

    Protein-protein interaction databases

    BioGridi113207. 38 interactions.
    IntActiQ16851. 6 interactions.
    MINTiMINT-1468909.
    STRINGi9606.ENSP00000338703.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3R2WX-ray3.60A/B/C/D1-508[»]
    3R3IX-ray3.57A/B/C/D1-508[»]
    ProteinModelPortaliQ16851.
    SMRiQ16851. Positions 22-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni457 – 50852OligomerizationAdd
    BLAST
    Regioni502 – 5032Critical for end-to-end subunit interaction

    Sequence similaritiesi

    Belongs to the UDPGP type 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG4284.
    HOGENOMiHOG000113618.
    HOVERGENiHBG055396.
    InParanoidiQ16851.
    KOiK00963.
    OrthoDBiEOG7ZPNK4.
    PhylomeDBiQ16851.
    TreeFamiTF300567.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR029044. Nucleotide-diphossugar_trans.
    IPR016267. UDPGP_trans.
    IPR002618. UDPGP_trans_fam.
    [Graphical view]
    PANTHERiPTHR11952. PTHR11952. 1 hit.
    PTHR11952:SF1. PTHR11952:SF1. 1 hit.
    PfamiPF01704. UDPGP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000806. UDPGP. 1 hit.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q16851-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSRFVQDLSK AMSQDGASQF QEVIRQELEL SVKKELEKIL TTASSHEFEH    50
    TKKDLDGFRK LFHRFLQEKG PSVDWGKIQR PPEDSIQPYE KIKARGLPDN 100
    ISSVLNKLVV VKLNGGLGTS MGCKGPKSLI GVRNENTFLD LTVQQIEHLN 150
    KTYNTDVPLV LMNSFNTDED TKKILQKYNH CRVKIYTFNQ SRYPRINKES 200
    LLPVAKDVSY SGENTEAWYP PGHGDIYASF YNSGLLDTFI GEGKEYIFVS 250
    NIDNLGATVD LYILNHLMNP PNGKRCEFVM EVTNKTRADV KGGTLTQYEG 300
    KLRLVEIAQV PKAHVDEFKS VSKFKIFNTN NLWISLAAVK RLQEQNAIDM 350
    EIIVNAKTLD GGLNVIQLET AVGAAIKSFE NSLGINVPRS RFLPVKTTSD 400
    LLLVMSNLYS LNAGSLTMSE KREFPTVPLV KLGSSFTKVQ DYLRRFESIP 450
    DMLELDHLTV SGDVTFGKNV SLKGTVIIIA NHGDRIDIPP GAVLENKIVS 500
    GNLRILDH 508
    Length:508
    Mass (Da):56,940
    Last modified:January 23, 2007 - v5
    Checksum:i60E54806807AB470
    GO
    Isoform 2 (identifier: Q16851-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-11: Missing.

    Show »
    Length:497
    Mass (Da):55,677
    Checksum:i2B3FD9731E371E47
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti25 – 251R → L(PubMed:8354390)Curated
    Sequence conflicti44 – 441S → T(PubMed:8354390)Curated
    Sequence conflicti48 – 481F → Y(PubMed:8354390)Curated
    Sequence conflicti62 – 621F → Y(PubMed:8354390)Curated
    Sequence conflicti152 – 1521T → S(PubMed:8354390)Curated
    Sequence conflicti202 – 2021L → R(PubMed:8354390)Curated
    Sequence conflicti202 – 2021L → R in AAB05640. (PubMed:8631325)Curated
    Sequence conflicti210 – 2101Y → S(PubMed:8354390)Curated
    Sequence conflicti214 – 2141N → S(PubMed:8354390)Curated
    Sequence conflicti240 – 2412IG → LE(PubMed:8354390)Curated
    Sequence conflicti356 – 3561A → P(PubMed:8354390)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti268 – 2681M → I.2 Publications
    Corresponds to variant rs1130982 [ dbSNP | Ensembl ].
    VAR_033042

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1111Missing in isoform 2. 2 PublicationsVSP_012834Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U27460 mRNA. Translation: AAB05640.1.
    BC000173 mRNA. Translation: AAH00173.2.
    BC002954 mRNA. Translation: AAH02954.1.
    BC047004 mRNA. Translation: AAH47004.1.
    CCDSiCCDS1875.1. [Q16851-1]
    CCDS42690.1. [Q16851-2]
    PIRiS35692.
    RefSeqiNP_001001521.1. NM_001001521.1. [Q16851-2]
    NP_006750.3. NM_006759.3. [Q16851-1]
    XP_005264594.1. XM_005264537.2. [Q16851-2]
    XP_005264595.1. XM_005264538.1. [Q16851-2]
    UniGeneiHs.516217.

    Genome annotation databases

    EnsembliENST00000337130; ENSP00000338703; ENSG00000169764. [Q16851-1]
    ENST00000394417; ENSP00000377939; ENSG00000169764. [Q16851-2]
    ENST00000467648; ENSP00000420793; ENSG00000169764. [Q16851-2]
    GeneIDi7360.
    KEGGihsa:7360.
    UCSCiuc002scl.3. human. [Q16851-1]

    Polymorphism databases

    DMDMi59803098.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U27460 mRNA. Translation: AAB05640.1 .
    BC000173 mRNA. Translation: AAH00173.2 .
    BC002954 mRNA. Translation: AAH02954.1 .
    BC047004 mRNA. Translation: AAH47004.1 .
    CCDSi CCDS1875.1. [Q16851-1 ]
    CCDS42690.1. [Q16851-2 ]
    PIRi S35692.
    RefSeqi NP_001001521.1. NM_001001521.1. [Q16851-2 ]
    NP_006750.3. NM_006759.3. [Q16851-1 ]
    XP_005264594.1. XM_005264537.2. [Q16851-2 ]
    XP_005264595.1. XM_005264538.1. [Q16851-2 ]
    UniGenei Hs.516217.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3R2W X-ray 3.60 A/B/C/D 1-508 [» ]
    3R3I X-ray 3.57 A/B/C/D 1-508 [» ]
    ProteinModelPortali Q16851.
    SMRi Q16851. Positions 22-500.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113207. 38 interactions.
    IntActi Q16851. 6 interactions.
    MINTi MINT-1468909.
    STRINGi 9606.ENSP00000338703.

    PTM databases

    PhosphoSitei Q16851.

    Polymorphism databases

    DMDMi 59803098.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00395676.
    UCD-2DPAGE Q16851.

    Proteomic databases

    MaxQBi Q16851.
    PaxDbi Q16851.
    PRIDEi Q16851.

    Protocols and materials databases

    DNASUi 7360.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000337130 ; ENSP00000338703 ; ENSG00000169764 . [Q16851-1 ]
    ENST00000394417 ; ENSP00000377939 ; ENSG00000169764 . [Q16851-2 ]
    ENST00000467648 ; ENSP00000420793 ; ENSG00000169764 . [Q16851-2 ]
    GeneIDi 7360.
    KEGGi hsa:7360.
    UCSCi uc002scl.3. human. [Q16851-1 ]

    Organism-specific databases

    CTDi 7360.
    GeneCardsi GC02P064068.
    HGNCi HGNC:12527. UGP2.
    HPAi HPA034696.
    MIMi 191750. gene.
    191760. gene.
    neXtProti NX_Q16851.
    PharmGKBi PA37172.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG4284.
    HOGENOMi HOG000113618.
    HOVERGENi HBG055396.
    InParanoidi Q16851.
    KOi K00963.
    OrthoDBi EOG7ZPNK4.
    PhylomeDBi Q16851.
    TreeFami TF300567.

    Enzyme and pathway databases

    BioCyci MetaCyc:HS10006-MONOMER.
    Reactomei REACT_169208. Glycogen synthesis.
    REACT_6737. Formation of the active cofactor, UDP-glucuronate.
    SABIO-RK Q16851.

    Miscellaneous databases

    ChiTaRSi UGP2. human.
    GenomeRNAii 7360.
    NextBioi 28818.
    PROi Q16851.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q16851.
    Bgeei Q16851.
    CleanExi HS_UGP2.
    Genevestigatori Q16851.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR029044. Nucleotide-diphossugar_trans.
    IPR016267. UDPGP_trans.
    IPR002618. UDPGP_trans_fam.
    [Graphical view ]
    PANTHERi PTHR11952. PTHR11952. 1 hit.
    PTHR11952:SF1. PTHR11952:SF1. 1 hit.
    Pfami PF01704. UDPGP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000806. UDPGP. 1 hit.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a human liver UDP-glucose pyrophosphorylase cDNA by complementation of the bacterial galU mutation."
      Peng H.-L., Chang H.-Y.
      FEBS Lett. 329:153-158(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, VARIANT ILE-268.
      Tissue: Liver.
    2. "Sequence differences between human muscle and liver cDNAs for UDPglucose pyrophosphorylase and kinetic properties of the recombinant enzymes expressed in Escherichia coli."
      Duggleby R.G., Chao Y.C., Huang J.G., Peng H.-L., Chang H.-Y.
      Eur. J. Biochem. 235:173-179(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT ILE-268.
      Tissue: Skeletal muscle.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Cervix, Lymph and Skin.
    4. "The importance of conserved residues in human liver UDPglucose pyrophosphorylase."
      Chang H.-Y., Peng H.-L., Chao Y.C., Duggleby R.G.
      Eur. J. Biochem. 236:723-728(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    5. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-438, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "The crystal structure of human UDP-glucose pyrophosphorylase reveals a latch effect that influences enzymatic activity."
      Yu Q., Zheng X.
      Biochem. J. 442:283-291(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.57 ANGSTROMS), SUBUNIT, MUTAGENESIS OF 502-ASP--LEU-503.

    Entry informationi

    Entry nameiUGPA_HUMAN
    AccessioniPrimary (citable) accession number: Q16851
    Secondary accession number(s): Q07131
    , Q0P6K2, Q86Y81, Q9BU15
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 128 of the entry and version 5 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    The human genome was initially thought to contain 2 genes for UTP--glucose-1-phosphate uridylyltransferase: UGP1 and UGP2. However, the sequence defined as UGP1 (PubMed:8354390) probably does not exist and corresponds to UGP2.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3