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Q16831

- UPP1_HUMAN

UniProt

Q16831 - UPP1_HUMAN

Protein

Uridine phosphorylase 1

Gene

UPP1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.

    Catalytic activityi

    Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei217 – 2171Substrate
    Binding sitei219 – 2191Substrate

    GO - Molecular functioni

    1. uridine phosphorylase activity Source: ProtInc

    GO - Biological processi

    1. cellular response to glucose starvation Source: Ensembl
    2. nucleobase-containing compound metabolic process Source: ProtInc
    3. nucleobase-containing small molecule metabolic process Source: Reactome
    4. nucleotide catabolic process Source: InterPro
    5. pyrimidine nucleobase metabolic process Source: Reactome
    6. pyrimidine nucleoside catabolic process Source: Reactome
    7. pyrimidine nucleoside salvage Source: Reactome
    8. small molecule metabolic process Source: Reactome
    9. UMP salvage Source: UniProtKB-UniPathway
    10. uridine metabolic process Source: Ensembl

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:HS00053-MONOMER.
    ReactomeiREACT_1023. Pyrimidine catabolism.
    REACT_655. Pyrimidine salvage reactions.
    SABIO-RKQ16831.
    SignaLinkiQ16831.
    UniPathwayiUPA00574; UER00633.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Uridine phosphorylase 1 (EC:2.4.2.3)
    Short name:
    UPase 1
    Short name:
    UrdPase 1
    Gene namesi
    Name:UPP1
    Synonyms:UP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:12576. UPP1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA365.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 310310Uridine phosphorylase 1PRO_0000063191Add
    BLAST

    Proteomic databases

    MaxQBiQ16831.
    PaxDbiQ16831.
    PeptideAtlasiQ16831.
    PRIDEiQ16831.

    PTM databases

    PhosphoSiteiQ16831.

    Expressioni

    Inductioni

    By vitamin D3 and a mixture of inflammatory cytokines: TNF, IL1/interleukin-1 and IFNG/IFN-gamma.

    Gene expression databases

    ArrayExpressiQ16831.
    BgeeiQ16831.
    CleanExiHS_UPP1.
    GenevestigatoriQ16831.

    Organism-specific databases

    HPAiHPA055394.

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Protein-protein interaction databases

    BioGridi113224. 10 interactions.
    IntActiQ16831. 1 interaction.
    STRINGi9606.ENSP00000330032.

    Structurei

    Secondary structure

    1
    310
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi26 – 283
    Turni35 – 384
    Turni41 – 433
    Helixi46 – 505
    Beta strandi55 – 606
    Helixi62 – 7615
    Beta strandi81 – 833
    Turni88 – 914
    Beta strandi97 – 1004
    Beta strandi103 – 1075
    Helixi112 – 12817
    Beta strandi135 – 14713
    Beta strandi152 – 1598
    Beta strandi165 – 1717
    Beta strandi174 – 1796
    Helixi184 – 19714
    Beta strandi202 – 2098
    Helixi215 – 2173
    Beta strandi222 – 2243
    Helixi229 – 24113
    Beta strandi244 – 2507
    Helixi251 – 26010
    Beta strandi264 – 27411
    Helixi275 – 2773
    Helixi285 – 2928
    Helixi294 – 30613

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3EUEX-ray2.30A1-310[»]
    3EUFX-ray1.90A/B/C/D1-310[»]
    3NBQX-ray2.30A/B/C/D1-310[»]
    ProteinModelPortaliQ16831.
    SMRiQ16831. Positions 16-309.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ16831.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PNP/UDP phosphorylase family.Curated

    Phylogenomic databases

    eggNOGiCOG2820.
    HOGENOMiHOG000231747.
    HOVERGENiHBG047725.
    InParanoidiQ16831.
    KOiK00757.
    OMAiYHAKCSN.
    PhylomeDBiQ16831.
    TreeFamiTF314310.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    InterProiIPR018017. Nucleoside_phosphorylase.
    IPR018016. Nucleoside_phosphorylase_CS.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR010059. Uridine_phosphorylase_euk.
    [Graphical view]
    PANTHERiPTHR21234. PTHR21234. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01719. euk_UDPppase. 1 hit.
    PROSITEiPS01232. PNP_UDP_1. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q16831-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAATGANAEK AESHNDCPVR LLNPNIAKMK EDILYHFNLT TSRHNFPALF    50
    GDVKFVCVGG SPSRMKAFIR CVGAELGLDC PGRDYPNICA GTDRYAMYKV 100
    GPVLSVSHGM GIPSISIMLH ELIKLLYYAR CSNVTIIRIG TSGGIGLEPG 150
    TVVITEQAVD TCFKAEFEQI VLGKRVIRKT DLNKKLVQEL LLCSAELSEF 200
    TTVVGNTMCT LDFYEGQGRL DGALCSYTEK DKQAYLEAAY AAGVRNIEME 250
    SSVFAAMCSA CGLQAAVVCV TLLNRLEGDQ ISSPRNVLSE YQQRPQRLVS 300
    YFIKKKLSKA 310
    Length:310
    Mass (Da):33,934
    Last modified:November 1, 1997 - v1
    Checksum:i110CF678561E53F3
    GO
    Isoform 2 (identifier: Q16831-2) [UniParc]FASTAAdd to Basket

    Also known as: Truncated

    The sequence of this isoform differs from the canonical sequence as follows:
         15-36: NDCPVRLLNPNIAKMKEDILYH → KSGARHCGHNRAGSGYLLQGRV
         37-310: Missing.

    Note: Inactive.

    Show »
    Length:36
    Mass (Da):3,694
    Checksum:i5D38DC6FA1548616
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei15 – 3622NDCPV…DILYH → KSGARHCGHNRAGSGYLLQG RV in isoform 2. 1 PublicationVSP_001279Add
    BLAST
    Alternative sequencei37 – 310274Missing in isoform 2. 1 PublicationVSP_001280Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X90858 mRNA. Translation: CAA62369.1.
    X90858 mRNA. Translation: CAA62370.1.
    BT006699 mRNA. Translation: AAP35345.1.
    CH471128 Genomic DNA. Translation: EAW60994.1.
    CH471128 Genomic DNA. Translation: EAW60995.1.
    BC001405 mRNA. Translation: AAH01405.1.
    BC007348 mRNA. Translation: AAH07348.1.
    BC053592 mRNA. Translation: AAH53592.1. Sequence problems.
    CCDSiCCDS5507.1. [Q16831-1]
    PIRiJC4343.
    RefSeqiNP_001274355.1. NM_001287426.1. [Q16831-1]
    NP_001274357.1. NM_001287428.1.
    NP_001274358.1. NM_001287429.1.
    NP_001274359.1. NM_001287430.1.
    NP_003355.1. NM_003364.3. [Q16831-1]
    UniGeneiHs.488240.

    Genome annotation databases

    EnsembliENST00000331803; ENSP00000330032; ENSG00000183696. [Q16831-1]
    ENST00000395560; ENSP00000378927; ENSG00000183696. [Q16831-2]
    ENST00000395564; ENSP00000378931; ENSG00000183696. [Q16831-1]
    ENST00000417464; ENSP00000413611; ENSG00000183696. [Q16831-2]
    ENST00000457596; ENSP00000408899; ENSG00000183696. [Q16831-2]
    GeneIDi7378.
    KEGGihsa:7378.
    UCSCiuc003toj.3. human. [Q16831-1]

    Polymorphism databases

    DMDMi2494059.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X90858 mRNA. Translation: CAA62369.1 .
    X90858 mRNA. Translation: CAA62370.1 .
    BT006699 mRNA. Translation: AAP35345.1 .
    CH471128 Genomic DNA. Translation: EAW60994.1 .
    CH471128 Genomic DNA. Translation: EAW60995.1 .
    BC001405 mRNA. Translation: AAH01405.1 .
    BC007348 mRNA. Translation: AAH07348.1 .
    BC053592 mRNA. Translation: AAH53592.1 . Sequence problems.
    CCDSi CCDS5507.1. [Q16831-1 ]
    PIRi JC4343.
    RefSeqi NP_001274355.1. NM_001287426.1. [Q16831-1 ]
    NP_001274357.1. NM_001287428.1.
    NP_001274358.1. NM_001287429.1.
    NP_001274359.1. NM_001287430.1.
    NP_003355.1. NM_003364.3. [Q16831-1 ]
    UniGenei Hs.488240.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3EUE X-ray 2.30 A 1-310 [» ]
    3EUF X-ray 1.90 A/B/C/D 1-310 [» ]
    3NBQ X-ray 2.30 A/B/C/D 1-310 [» ]
    ProteinModelPortali Q16831.
    SMRi Q16831. Positions 16-309.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113224. 10 interactions.
    IntActi Q16831. 1 interaction.
    STRINGi 9606.ENSP00000330032.

    Chemistry

    BindingDBi Q16831.
    ChEMBLi CHEMBL4811.

    PTM databases

    PhosphoSitei Q16831.

    Polymorphism databases

    DMDMi 2494059.

    Proteomic databases

    MaxQBi Q16831.
    PaxDbi Q16831.
    PeptideAtlasi Q16831.
    PRIDEi Q16831.

    Protocols and materials databases

    DNASUi 7378.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000331803 ; ENSP00000330032 ; ENSG00000183696 . [Q16831-1 ]
    ENST00000395560 ; ENSP00000378927 ; ENSG00000183696 . [Q16831-2 ]
    ENST00000395564 ; ENSP00000378931 ; ENSG00000183696 . [Q16831-1 ]
    ENST00000417464 ; ENSP00000413611 ; ENSG00000183696 . [Q16831-2 ]
    ENST00000457596 ; ENSP00000408899 ; ENSG00000183696 . [Q16831-2 ]
    GeneIDi 7378.
    KEGGi hsa:7378.
    UCSCi uc003toj.3. human. [Q16831-1 ]

    Organism-specific databases

    CTDi 7378.
    GeneCardsi GC07P048094.
    HGNCi HGNC:12576. UPP1.
    HPAi HPA055394.
    MIMi 191730. gene.
    neXtProti NX_Q16831.
    PharmGKBi PA365.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2820.
    HOGENOMi HOG000231747.
    HOVERGENi HBG047725.
    InParanoidi Q16831.
    KOi K00757.
    OMAi YHAKCSN.
    PhylomeDBi Q16831.
    TreeFami TF314310.

    Enzyme and pathway databases

    UniPathwayi UPA00574 ; UER00633 .
    BioCyci MetaCyc:HS00053-MONOMER.
    Reactomei REACT_1023. Pyrimidine catabolism.
    REACT_655. Pyrimidine salvage reactions.
    SABIO-RK Q16831.
    SignaLinki Q16831.

    Miscellaneous databases

    ChiTaRSi UPP1. human.
    EvolutionaryTracei Q16831.
    GeneWikii UPP1.
    GenomeRNAii 7378.
    NextBioi 28890.
    PROi Q16831.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q16831.
    Bgeei Q16831.
    CleanExi HS_UPP1.
    Genevestigatori Q16831.

    Family and domain databases

    Gene3Di 3.40.50.1580. 1 hit.
    InterProi IPR018017. Nucleoside_phosphorylase.
    IPR018016. Nucleoside_phosphorylase_CS.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR010059. Uridine_phosphorylase_euk.
    [Graphical view ]
    PANTHERi PTHR21234. PTHR21234. 1 hit.
    Pfami PF01048. PNP_UDP_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53167. SSF53167. 1 hit.
    TIGRFAMsi TIGR01719. euk_UDPppase. 1 hit.
    PROSITEi PS01232. PNP_UDP_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of human uridine phosphorylase."
      Watanabe S., Uchida T.
      Biochem. Biophys. Res. Commun. 216:265-272(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
      Tissue: Colon.
    2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Ovary, Placenta and Skin.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Implications of the structure of human uridine phosphorylase 1 on the development of novel inhibitors for improving the therapeutic window of fluoropyrimidine chemotherapy."
      Roosild T.P., Castronovo S., Fabbiani M., Pizzorno G.
      BMC Struct. Biol. 9:14-14(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) ALONE AND IN COMPLEX WITH INHIBITOR, SUBUNIT.
    7. "Active site conformational dynamics in human uridine phosphorylase 1."
      Roosild T.P., Castronovo S.
      PLoS ONE 5:E12741-E12741(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH 5-FLUOROURACIL, SUBSTRATE-BINDING SITES, SUBUNIT.

    Entry informationi

    Entry nameiUPP1_HUMAN
    AccessioniPrimary (citable) accession number: Q16831
    Secondary accession number(s): D3DVM4, Q15362
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3