Q16829 (DUS7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual specificity protein phosphatase 7
EC=3.1.3.16
EC=3.1.3.48

Alternative name(s):
Dual specificity protein phosphatase PYST2

Gene names

Name:	DUSP7
Synonyms:	PYST2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates the activity of the MAP kinase family in response to changes in the cellular environment. PYST2-S may act as a negative regulator of PYST2-L although it is unclear whether this is by competing for transcription, translation or activation factors. Ref.1
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Subcellular location	Cytoplasm.
Tissue specificity	Expressed at significantly higher levels in malignant hematopoietic cells than in corresponding non-malignant cells. Ref.6
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 rhodanese domain. Contains 1 tyrosine-protein phosphatase domain.
Caution	An out-of-frame translation product, PYST2SB, has been experimentally demonstrated to be formed from the alternative promoter. The expression of the in-frame product has not yet been shown.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative promoter usage Polymorphism
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	MyD88-dependent toll-like receptor signaling pathway Traceable author statement. Source: Reactome TRIF-dependent toll-like receptor signaling pathway Traceable author statement. Source: Reactome Toll signaling pathway Traceable author statement. Source: Reactome innate immune response Traceable author statement. Source: Reactome nerve growth factor receptor signaling pathway Traceable author statement. Source: Reactome peptidyl-tyrosine dephosphorylation Inferred from direct assay PubMed 9788880. Source: UniProtKB stress-activated MAPK cascade Traceable author statement. Source: Reactome toll-like receptor 1 signaling pathway Traceable author statement. Source: Reactome toll-like receptor 2 signaling pathway Traceable author statement. Source: Reactome toll-like receptor 3 signaling pathway Traceable author statement. Source: Reactome toll-like receptor 4 signaling pathway Traceable author statement. Source: Reactome
Cellular_component	cytosol Inferred from direct assay PubMed 9788880. Source: UniProtKB nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	MAP kinase tyrosine/serine/threonine phosphatase activity Inferred from direct assay PubMed 9788880. Source: UniProtKB protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GHR	P10912	2	EBI-1265847,EBI-286316

Alternative products

This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]

Isoform 1 (identifier: Q16829-1)

Also known as: PYST2-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q16829-2)

Also known as: PYST2-S;

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Dual specificity protein phosphatase 7		PRO_0000094807
Regions
	Domain	68 – 187	120	Rhodanese
	Domain	308 – 376	69	Tyrosine-protein phosphatase
	Compositional bias	192 – 240	49	Ser-rich
Sites
	Active site	331	1	Phosphocysteine intermediate By similarity
Natural variations
	Alternative sequence	1 – 51	51	Missing in isoform 2.		VSP_012822
	Natural variant	235	1	S → N. Corresponds to variant rs34821455 [ dbSNP | Ensembl ].		VAR_051752

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (PYST2-L) [UniParc]. Last modified June 28, 2011. Version 4. Checksum: 434467AC16A3F8BA	FASTA	419	44,957
	10 20 30 40 50 60 MKNQLRGPPA RAHMSTSGAA AAGGTRAGSE PGAGSGSGAG TGAGAATGAG AMPCKSAEWL 70 80 90 100 110 120 QEELEARGGA SLLLLDCRPH ELFESSHIET AINLAIPGLM LRRLRKGNLP IRSIIPNHAD 130 140 150 160 170 180 KERFATRCKA ATVLLYDEAT AEWQPEPGAP ASVLGLLLQK LRDDGCQAYY LQGGFNKFQT 190 200 210 220 230 240 EYSEHCETNV DSSSSPSSSP PTSVLGLGGL RISSDCSDGE SDRELPSSAT ESDGSPVPSS 250 260 270 280 290 300 QPAFPVQILP YLYLGCAKDS TNLDVLGKYG IKYILNVTPN LPNAFEHGGE FTYKQIPISD 310 320 330 340 350 360 HWSQNLSQFF PEAISFIDEA RSKKCGVLVH CLAGISRSVT VTVAYLMQKM NLSLNDAYDF 370 380 390 400 410 VKRKKSNISP NFNFMGQLLD FERTLGLSSP CDNHASSEQL YFSTPTNHNL FPLNTLEST
	Isoform 2 (PYST2-S) [UniParc]. Checksum: 097023C27EBC341C	FASTA	368	40,551
	10 20 30 40 50 60 MPCKSAEWLQ EELEARGGAS LLLLDCRPHE LFESSHIETA INLAIPGLML RRLRKGNLPI 70 80 90 100 110 120 RSIIPNHADK ERFATRCKAA TVLLYDEATA EWQPEPGAPA SVLGLLLQKL RDDGCQAYYL 130 140 150 160 170 180 QGGFNKFQTE YSEHCETNVD SSSSPSSSPP TSVLGLGGLR ISSDCSDGES DRELPSSATE 190 200 210 220 230 240 SDGSPVPSSQ PAFPVQILPY LYLGCAKDST NLDVLGKYGI KYILNVTPNL PNAFEHGGEF 250 260 270 280 290 300 TYKQIPISDH WSQNLSQFFP EAISFIDEAR SKKCGVLVHC LAGISRSVTV TVAYLMQKMN 310 320 330 340 350 360 LSLNDAYDFV KRKKSNISPN FNFMGQLLDF ERTLGLSSPC DNHASSEQLY FSTPTNHNLF PLNTLEST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the dual-specificity phosphatase PYST2 and its transcripts." Levy-Nissenbaum O., Sagi-Assif O., Witz I.P. Genes Chromosomes Cancer 39:37-47(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE PROMOTER USAGE, FUNCTION. Tissue: Monocyte.
[2]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Full-length cDNA libraries and normalization." Li W.B., Gruber C., Jessee J., Polayes D. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-235 (ISOFORM 1).
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 83-419 (ISOFORM 1). Tissue: Brain and Lymph.
[5]	"Differential regulation of the MAP, SAP and RK/p38 kinases by Pyst1, a novel cytosolic dual-specificity phosphatase." Groom L.A., Sneddon A.A., Alessi D.R., Dowd S., Keyse S.M. EMBO J. 15:3621-3632(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 149-419 (ISOFORM 1). Tissue: Testis.
[6]	"Dual-specificity phosphatase Pyst2-L is constitutively highly expressed in myeloid leukemia and other malignant cells." Levy-Nissenbaum O., Sagi-Assif O., Kapon D., Hantisteanu S., Burg T., Raanani P., Avigdor A., Ben-Bassat I., Witz I.P. Oncogene 22:7649-7660(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF508727 mRNA. Translation: AAM77606.1. AF508728 mRNA. No translation available. AC115284 Genomic DNA. No translation available. AL556300 mRNA. No translation available. BC019107 mRNA. Translation: AAH19107.2. BC104880 mRNA. Translation: AAI04881.1. BC104882 mRNA. Translation: AAI04883.1. X93921 mRNA. Translation: CAA63814.1.
IPI	IPI00394738. IPI00944963.
RefSeq	NP_001938.2. NM_001947.3.
UniGene	Hs.591664.
3D structure databases
ProteinModelPortal	Q16829.
SMR	Q16829. Positions 55-193, 243-385.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q16829. 1 interaction.
STRING	9606.ENSP00000296483.
PTM databases
PhosphoSite	Q16829.
Polymorphism databases
DMDM	59802887.
Proteomic databases
PaxDb	Q16829.
PRIDE	Q16829.
Protocols and materials databases
DNASU	1849.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000296483; ENSP00000296483; ENSG00000164086. ENST00000495880; ENSP00000417183; ENSG00000164086.
GeneID	1849.
KEGG	hsa:1849.
UCSC	uc003dct.3. human.
Organism-specific databases
CTD	1849.
GeneCards	GC03M052082.
H-InvDB	HIX0003343.
HGNC	HGNC:3073. DUSP7.
MIM	602749. gene.
neXtProt	NX_Q16829.
PharmGKB	PA27530.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2453.
HOGENOM	HOG000294079.
HOVERGEN	HBG007347.
InParanoid	Q16829.
KO	K04459.
OMA	LRDDGCK.
OrthoDB	EOG4GB76F.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction. REACT_6782. TRAF6 Mediated Induction of proinflammatory cytokines. REACT_6900. Immune System.
Gene expression databases
ArrayExpress	Q16829.
Bgee	Q16829.
CleanEx	HS_DUSP7.
Genevestigator	Q16829.
GermOnline	ENSG00000164086. Homo sapiens.
Family and domain databases
Gene3D	3.40.250.10. 1 hit.
InterPro	IPR000340. Dual-sp_phosphatase_cat-dom. IPR020422. Dual-sp_phosphatase_subgr_cat. IPR024950. DUSP. IPR008343. MKP. IPR001763. Rhodanese-like_dom. IPR000387. Tyr/Dual-sp_Pase. [Graphical view]
PANTHER	PTHR10159. PTHR10159. 1 hit.
Pfam	PF00782. DSPc. 1 hit. PF00581. Rhodanese. 1 hit. [Graphical view]
PIRSF	PIRSF000939. MAPK_Ptase. 1 hit.
PRINTS	PR01764. MAPKPHPHTASE.
SMART	SM00195. DSPc. 1 hit. SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. PS00383. TYR_PHOSPHATASE_1. False negative. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	1849.
NextBio	7575.
SOURCE	Search...

Entry information

Entry name

DUS7_HUMAN

Accession

Primary (citable) accession number: Q16829
Secondary accession number(s): Q2M3J7, Q8NFJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 28, 2011
Last modified:	May 1, 2013
This is version 112 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.