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Q16829 (DUS7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dual specificity protein phosphatase 7

EC=3.1.3.16
EC=3.1.3.48
Alternative name(s):
Dual specificity protein phosphatase PYST2
Gene names
Name:DUSP7
Synonyms:PYST2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length419 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates the activity of the MAP kinase family in response to changes in the cellular environment. PYST2-S may act as a negative regulator of PYST2-L although it is unclear whether this is by competing for transcription, translation or activation factors. Ref.1

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Subcellular location

Cytoplasm.

Tissue specificity

Expressed at significantly higher levels in malignant hematopoietic cells than in corresponding non-malignant cells. Ref.6

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Contains 1 rhodanese domain.

Contains 1 tyrosine-protein phosphatase domain.

Caution

An out-of-frame translation product, PYST2SB, has been experimentally demonstrated to be formed from the alternative promoter. The expression of the in-frame product has not yet been shown.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative promoter usage
Polymorphism
   Molecular functionHydrolase
Protein phosphatase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processMyD88-dependent toll-like receptor signaling pathway

Traceable author statement. Source: Reactome

MyD88-independent toll-like receptor signaling pathway

Traceable author statement. Source: Reactome

TRIF-dependent toll-like receptor signaling pathway

Traceable author statement. Source: Reactome

inactivation of MAPK activity

Inferred from direct assay PubMed 9788880. Source: GOC

innate immune response

Traceable author statement. Source: Reactome

negative regulation of MAP kinase activity

Inferred from direct assay PubMed 9788880. Source: UniProtKB

neurotrophin TRK receptor signaling pathway

Traceable author statement. Source: Reactome

peptidyl-tyrosine dephosphorylation

Inferred from direct assay PubMed 9788880. Source: UniProtKB

stress-activated MAPK cascade

Traceable author statement. Source: Reactome

toll-like receptor 10 signaling pathway

Traceable author statement. Source: Reactome

toll-like receptor 2 signaling pathway

Traceable author statement. Source: Reactome

toll-like receptor 3 signaling pathway

Traceable author statement. Source: Reactome

toll-like receptor 4 signaling pathway

Traceable author statement. Source: Reactome

toll-like receptor 5 signaling pathway

Traceable author statement. Source: Reactome

toll-like receptor 9 signaling pathway

Traceable author statement. Source: Reactome

toll-like receptor TLR1:TLR2 signaling pathway

Traceable author statement. Source: Reactome

toll-like receptor TLR6:TLR2 signaling pathway

Traceable author statement. Source: Reactome

toll-like receptor signaling pathway

Traceable author statement. Source: Reactome

   Cellular_componentcytosol

Inferred from direct assay PubMed 9788880. Source: UniProtKB

nucleoplasm

Traceable author statement. Source: Reactome

   Molecular_functionMAP kinase tyrosine/serine/threonine phosphatase activity

Inferred from direct assay PubMed 9788880. Source: UniProtKB

protein binding

Inferred from physical interaction PubMed 12907755. Source: IntAct

protein tyrosine phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GHRP109122EBI-1265847,EBI-286316

Alternative products

This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]
Isoform 1 (identifier: Q16829-1)

Also known as: PYST2-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q16829-2)

Also known as: PYST2-S;

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 419419Dual specificity protein phosphatase 7
PRO_0000094807

Regions

Domain68 – 187120Rhodanese
Domain308 – 37669Tyrosine-protein phosphatase
Compositional bias192 – 24049Ser-rich

Sites

Active site3311Phosphocysteine intermediate By similarity

Natural variations

Alternative sequence1 – 5151Missing in isoform 2.
VSP_012822
Natural variant2351S → N.
Corresponds to variant rs34821455 [ dbSNP | Ensembl ].
VAR_051752

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (PYST2-L) [UniParc].

Last modified June 28, 2011. Version 4.
Checksum: 434467AC16A3F8BA

FASTA41944,957
        10         20         30         40         50         60 
MKNQLRGPPA RAHMSTSGAA AAGGTRAGSE PGAGSGSGAG TGAGAATGAG AMPCKSAEWL 

        70         80         90        100        110        120 
QEELEARGGA SLLLLDCRPH ELFESSHIET AINLAIPGLM LRRLRKGNLP IRSIIPNHAD 

       130        140        150        160        170        180 
KERFATRCKA ATVLLYDEAT AEWQPEPGAP ASVLGLLLQK LRDDGCQAYY LQGGFNKFQT 

       190        200        210        220        230        240 
EYSEHCETNV DSSSSPSSSP PTSVLGLGGL RISSDCSDGE SDRELPSSAT ESDGSPVPSS 

       250        260        270        280        290        300 
QPAFPVQILP YLYLGCAKDS TNLDVLGKYG IKYILNVTPN LPNAFEHGGE FTYKQIPISD 

       310        320        330        340        350        360 
HWSQNLSQFF PEAISFIDEA RSKKCGVLVH CLAGISRSVT VTVAYLMQKM NLSLNDAYDF 

       370        380        390        400        410 
VKRKKSNISP NFNFMGQLLD FERTLGLSSP CDNHASSEQL YFSTPTNHNL FPLNTLEST 

« Hide

Isoform 2 (PYST2-S) [UniParc].

Checksum: 097023C27EBC341C
Show »

FASTA36840,551

References

« Hide 'large scale' references
[1]"Characterization of the dual-specificity phosphatase PYST2 and its transcripts."
Levy-Nissenbaum O., Sagi-Assif O., Witz I.P.
Genes Chromosomes Cancer 39:37-47(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE PROMOTER USAGE, FUNCTION.
Tissue: Monocyte.
[2]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Full-length cDNA libraries and normalization."
Li W.B., Gruber C., Jessee J., Polayes D.
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-235 (ISOFORM 1).
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 83-419 (ISOFORM 1).
Tissue: Brain and Lymph.
[5]"Differential regulation of the MAP, SAP and RK/p38 kinases by Pyst1, a novel cytosolic dual-specificity phosphatase."
Groom L.A., Sneddon A.A., Alessi D.R., Dowd S., Keyse S.M.
EMBO J. 15:3621-3632(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 149-419 (ISOFORM 1).
Tissue: Testis.
[6]"Dual-specificity phosphatase Pyst2-L is constitutively highly expressed in myeloid leukemia and other malignant cells."
Levy-Nissenbaum O., Sagi-Assif O., Kapon D., Hantisteanu S., Burg T., Raanani P., Avigdor A., Ben-Bassat I., Witz I.P.
Oncogene 22:7649-7660(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF508727 mRNA. Translation: AAM77606.1.
AF508728 mRNA. No translation available.
AC115284 Genomic DNA. No translation available.
AL556300 mRNA. No translation available.
BC019107 mRNA. Translation: AAH19107.2.
BC104880 mRNA. Translation: AAI04881.1.
BC104882 mRNA. Translation: AAI04883.1.
X93921 mRNA. Translation: CAA63814.1.
CCDSCCDS33766.2. [Q16829-1]
RefSeqNP_001938.2. NM_001947.3. [Q16829-1]
UniGeneHs.591664.

3D structure databases

ProteinModelPortalQ16829.
SMRQ16829. Positions 55-193, 243-385.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108182. 4 interactions.
IntActQ16829. 4 interactions.
STRING9606.ENSP00000296483.

PTM databases

PhosphoSiteQ16829.

Polymorphism databases

DMDM338817906.

Proteomic databases

MaxQBQ16829.
PaxDbQ16829.
PRIDEQ16829.

Protocols and materials databases

DNASU1849.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000296483; ENSP00000296483; ENSG00000164086. [Q16829-2]
ENST00000495880; ENSP00000417183; ENSG00000164086. [Q16829-1]
GeneID1849.
KEGGhsa:1849.
UCSCuc003dct.3. human. [Q16829-1]

Organism-specific databases

CTD1849.
GeneCardsGC03M052082.
H-InvDBHIX0003343.
HGNCHGNC:3073. DUSP7.
MIM602749. gene.
neXtProtNX_Q16829.
PharmGKBPA27530.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2453.
HOGENOMHOG000294079.
HOVERGENHBG007347.
InParanoidQ16829.
KOK04459.
OMALRDDGCK.
OrthoDBEOG793B7W.
PhylomeDBQ16829.
TreeFamTF105122.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_6782. TRAF6 Mediated Induction of proinflammatory cytokines.
REACT_6900. Immune System.

Gene expression databases

ArrayExpressQ16829.
BgeeQ16829.
CleanExHS_DUSP7.
GenevestigatorQ16829.

Family and domain databases

Gene3D3.40.250.10. 1 hit.
3.90.190.10. 1 hit.
InterProIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR008343. MKP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR001763. Rhodanese-like_dom.
IPR000387. Tyr/Dual-sp_Pase.
[Graphical view]
PANTHERPTHR10159. PTHR10159. 1 hit.
PfamPF00782. DSPc. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PIRSFPIRSF000939. MAPK_Ptase. 1 hit.
PRINTSPR01764. MAPKPHPHTASE.
SMARTSM00195. DSPc. 1 hit.
SM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMSSF52799. SSF52799. 1 hit.
SSF52821. SSF52821. 1 hit.
PROSITEPS50206. RHODANESE_3. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiDUSP7.
GenomeRNAi1849.
NextBio7575.
PROQ16829.
SOURCESearch...

Entry information

Entry nameDUS7_HUMAN
AccessionPrimary (citable) accession number: Q16829
Secondary accession number(s): Q2M3J7, Q8NFJ0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 28, 2011
Last modified: July 9, 2014
This is version 123 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM