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Protein

Dual specificity protein phosphatase 7

Gene

DUSP7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dual specificity protein phosphatase (PubMed:9788880). Shows high activity towards MAPK1/ERK2 (PubMed:9788880). Also has lower activity towards MAPK14 and MAPK8 (PubMed:9788880). In arrested oocytes, plays a role in meiotic resumption (By similarity). Promotes nuclear envelope breakdown and activation of the CDK1/Cyclin-B complex in oocytes, probably by dephosphorylating and inactivating the conventional protein kinase C (cPKC) isozyme PRKCB (By similarity). May also inactivate PRKCA and/or PRKCG (By similarity). Also important in oocytes for normal chromosome alignment on the metaphase plate and progression to anaphase, where it might regulate activity of the spindle-assembly checkpoint (SAC) complex (By similarity).By similarity1 Publication

Caution

An out-of-frame translation product, PYST2SB, has been experimentally demonstrated to be formed from the alternative promoter. The expression of the in-frame product has not yet been shown.Curated

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Enzyme regulationi

Strongly inhibited by sodium orthovanadate.1 Publication

Kineticsi

  1. KM=6.82 mM for p-nitrophenyl phosphate1 Publication
  2. KM=0.76 mM for p-nitrophenyl phosphate (in the presence of MAPK1)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei331Phosphocysteine intermediateBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    • activation of MAPK activity Source: Reactome
    • MAPK cascade Source: Reactome
    • negative regulation of MAP kinase activity Source: UniProtKB
    • peptidyl-tyrosine dephosphorylation Source: UniProtKB

    Keywordsi

    Molecular functionHydrolase, Protein phosphatase

    Enzyme and pathway databases

    ReactomeiR-HSA-112409 RAF-independent MAPK1/3 activation
    R-HSA-202670 ERKs are inactivated
    R-HSA-5675221 Negative regulation of MAPK pathway
    SIGNORiQ16829

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein phosphatase 7Imported (EC:3.1.3.161 Publication, EC:3.1.3.481 Publication)
    Alternative name(s):
    Dual specificity protein phosphatase PYST21 Publication
    Gene namesi
    Name:DUSP7Imported
    Synonyms:PYST21 Publication
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 3

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000164086.9
    HGNCiHGNC:3073 DUSP7
    MIMi602749 gene
    neXtProtiNX_Q16829

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi1849
    OpenTargetsiENSG00000164086
    PharmGKBiPA27530

    Polymorphism and mutation databases

    BioMutaiDUSP7
    DMDMi338817906

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000948071 – 419Dual specificity protein phosphatase 7Add BLAST419

    Proteomic databases

    MaxQBiQ16829
    PaxDbiQ16829
    PeptideAtlasiQ16829
    PRIDEiQ16829
    ProteomicsDBi61091
    61092 [Q16829-2]
    TopDownProteomicsiQ16829-2 [Q16829-2]

    PTM databases

    DEPODiQ16829
    iPTMnetiQ16829
    PhosphoSitePlusiQ16829

    Expressioni

    Tissue specificityi

    Strongly expressed in liver (PubMed:8670865). Expressed at significantly higher levels in malignant hematopoietic cells than in corresponding non-malignant cells (PubMed:14576828).2 Publications

    Gene expression databases

    BgeeiENSG00000164086
    CleanExiHS_DUSP7
    ExpressionAtlasiQ16829 baseline and differential
    GenevisibleiQ16829 HS

    Interactioni

    Subunit structurei

    Interacts with MAPK1/ERK2; the interaction enhances DUSP7 phosphatase activity.1 Publication

    Binary interactionsi

    Show more details

    Protein-protein interaction databases

    BioGridi108182, 13 interactors
    IntActiQ16829, 13 interactors
    MINTiQ16829
    STRINGi9606.ENSP00000417183

    Structurei

    Secondary structure

    1419
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi246 – 249Combined sources4
    Beta strandi252 – 255Combined sources4
    Helixi257 – 260Combined sources4
    Helixi263 – 268Combined sources6
    Beta strandi271 – 276Combined sources6
    Beta strandi279 – 281Combined sources3
    Beta strandi287 – 290Combined sources4
    Beta strandi292 – 295Combined sources4
    Helixi307 – 309Combined sources3
    Helixi310 – 322Combined sources13
    Beta strandi326 – 330Combined sources5
    Beta strandi332 – 336Combined sources5
    Helixi337 – 349Combined sources13
    Helixi354 – 364Combined sources11
    Helixi372 – 385Combined sources14

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4Y2EX-ray1.67A/B/C/D240-388[»]
    ProteinModelPortaliQ16829
    SMRiQ16829
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini68 – 187RhodanesePROSITE-ProRule annotationAdd BLAST120
    Domaini308 – 376Tyrosine-protein phosphataseSequence analysisAdd BLAST69

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni331 – 337Substrate bindingCombined sources1 Publication7

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi192 – 240Ser-richPROSITE-ProRule annotationAdd BLAST49

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG1717 Eukaryota
    COG2453 LUCA
    GeneTreeiENSGT00760000118902
    HOGENOMiHOG000294079
    HOVERGENiHBG007347
    InParanoidiQ16829
    KOiK04459
    OMAiRSKKCGI
    OrthoDBiEOG091G0249
    PhylomeDBiQ16829
    TreeFamiTF105122

    Family and domain databases

    CDDicd00127 DSPc, 1 hit
    Gene3Di3.40.250.10, 1 hit
    3.90.190.10, 1 hit
    InterProiView protein in InterPro
    IPR000340 Dual-sp_phosphatase_cat-dom
    IPR024950 DUSP
    IPR008343 MKP
    IPR029021 Prot-tyrosine_phosphatase-like
    IPR001763 Rhodanese-like_dom
    IPR036873 Rhodanese-like_dom_sf
    IPR000387 TYR_PHOSPHATASE_dom
    IPR020422 TYR_PHOSPHATASE_DUAL_dom
    PANTHERiPTHR10159 PTHR10159, 1 hit
    PfamiView protein in Pfam
    PF00782 DSPc, 1 hit
    PF00581 Rhodanese, 1 hit
    PIRSFiPIRSF000939 MAPK_Ptase, 1 hit
    PRINTSiPR01764 MAPKPHPHTASE
    SMARTiView protein in SMART
    SM00195 DSPc, 1 hit
    SM00450 RHOD, 1 hit
    SUPFAMiSSF52799 SSF52799, 1 hit
    SSF52821 SSF52821, 1 hit
    PROSITEiView protein in PROSITE
    PS50206 RHODANESE_3, 1 hit
    PS50056 TYR_PHOSPHATASE_2, 1 hit
    PS50054 TYR_PHOSPHATASE_DUAL, 1 hit

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

    Isoform 1 (identifier: Q16829-1) [UniParc]FASTAAdd to basket
    Also known as: PYST2-L

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MKNQLRGPPA RAHMSTSGAA AAGGTRAGSE PGAGSGSGAG TGAGAATGAG
    60 70 80 90 100
    AMPCKSAEWL QEELEARGGA SLLLLDCRPH ELFESSHIET AINLAIPGLM
    110 120 130 140 150
    LRRLRKGNLP IRSIIPNHAD KERFATRCKA ATVLLYDEAT AEWQPEPGAP
    160 170 180 190 200
    ASVLGLLLQK LRDDGCQAYY LQGGFNKFQT EYSEHCETNV DSSSSPSSSP
    210 220 230 240 250
    PTSVLGLGGL RISSDCSDGE SDRELPSSAT ESDGSPVPSS QPAFPVQILP
    260 270 280 290 300
    YLYLGCAKDS TNLDVLGKYG IKYILNVTPN LPNAFEHGGE FTYKQIPISD
    310 320 330 340 350
    HWSQNLSQFF PEAISFIDEA RSKKCGVLVH CLAGISRSVT VTVAYLMQKM
    360 370 380 390 400
    NLSLNDAYDF VKRKKSNISP NFNFMGQLLD FERTLGLSSP CDNHASSEQL
    410
    YFSTPTNHNL FPLNTLEST
    Length:419
    Mass (Da):44,957
    Last modified:June 28, 2011 - v4
    Checksum:i434467AC16A3F8BA
    GO
    Isoform 2 (identifier: Q16829-2) [UniParc]FASTAAdd to basket
    Also known as: PYST2-S

    The sequence of this isoform differs from the canonical sequence as follows:
         1-51: Missing.

    Show »
    Length:368
    Mass (Da):40,551
    Checksum:i097023C27EBC341C
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti87 – 90HIET → THRD no nucleotide entry (PubMed:9788880).Curated4

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_051752235S → N. Corresponds to variant dbSNP:rs34821455Ensembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0128221 – 51Missing in isoform 2. 1 PublicationAdd BLAST51

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF508727 mRNA Translation: AAM77606.1
    AF508728 mRNA No translation available.
    AC115284 Genomic DNA No translation available.
    AL556300 mRNA No translation available.
    BC019107 mRNA Translation: AAH19107.2
    BC104880 mRNA Translation: AAI04881.1
    BC104882 mRNA Translation: AAI04883.1
    X93921 mRNA Translation: CAA63814.1
    CCDSiCCDS33766.2 [Q16829-1]
    RefSeqiNP_001938.2, NM_001947.3 [Q16829-1]
    UniGeneiHs.591664

    Genome annotation databases

    EnsembliENST00000495880; ENSP00000417183; ENSG00000164086 [Q16829-1]
    GeneIDi1849
    KEGGihsa:1849
    UCSCiuc003dct.4 human [Q16829-1]

    Keywords - Coding sequence diversityi

    Alternative promoter usage, Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiDUS7_HUMAN
    AccessioniPrimary (citable) accession number: Q16829
    Secondary accession number(s): Q2M3J7, Q8NFJ0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: June 28, 2011
    Last modified: June 20, 2018
    This is version 151 of the entry and version 4 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

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