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Protein

Receptor-type tyrosine-protein phosphatase O

Gene

PTPRO

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Possesses tyrosine phosphatase activity. Plays a role in regulating the glomerular pressure/filtration rate relationship through an effect on podocyte structure and function (By similarity).By similarity1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1102SubstrateBy similarity1
Active sitei1136Phosphocysteine intermediatePROSITE-ProRule annotation1
Binding sitei1180SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase, Receptor

Enzyme and pathway databases

BRENDAi3.1.3.48 2681

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase O (EC:3.1.3.48)
Short name:
R-PTP-O
Alternative name(s):
Glomerular epithelial protein 1
Protein tyrosine phosphatase U2
Short name:
PTP-U2
Short name:
PTPase U2
Gene namesi
Name:PTPRO
Synonyms:GLEPP1, PTPU2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000151490.13
HGNCiHGNC:9678 PTPRO
MIMi600579 gene
neXtProtiNX_Q16827

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 822ExtracellularSequence analysisAdd BLAST793
Transmembranei823 – 843HelicalSequence analysisAdd BLAST21
Topological domaini844 – 1216CytoplasmicSequence analysisAdd BLAST373

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

Nephrotic syndrome 6 (NPHS6)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of nephrotic syndrome, a renal disease clinically characterized by severe proteinuria, resulting in complications such as hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure.
See also OMIM:614196

Organism-specific databases

DisGeNETi5800
MalaCardsiPTPRO
MIMi614196 phenotype
OpenTargetsiENSG00000151490
Orphaneti93214 Familial idiopathic steroid-resistant nephrotic syndrome with diffuse mesangial proliferation
93217 Familial idiopathic steroid-resistant nephrotic syndrome with diffuse mesangial sclerosis
93213 Familial idiopathic steroid-resistant nephrotic syndrome with focal segmental hyalinosis
93216 Familial idiopathic steroid-resistant nephrotic syndrome with minimal changes
PharmGKBiPA34023

Polymorphism and mutation databases

BioMutaiPTPRO
DMDMi209572663

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000002545830 – 1216Receptor-type tyrosine-protein phosphatase OAdd BLAST1187

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi75N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi154N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi189N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi201N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi227N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi278N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi287N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi323N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi324N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi370N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi461N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi490N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi700N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi712N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi733N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei865PhosphoserineBy similarity1
Modified residuei1210PhosphotyrosineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ16827
PaxDbiQ16827
PeptideAtlasiQ16827
PRIDEiQ16827
ProteomicsDBi61085
61086 [Q16827-2]
61087 [Q16827-3]
61088 [Q16827-4]

PTM databases

DEPODiQ16827
iPTMnetiQ16827
PhosphoSitePlusiQ16827

Expressioni

Tissue specificityi

Glomerulus of kidney. Also detected in brain, lung and placenta.1 Publication

Inductioni

By various differentiation-inducing agents.

Gene expression databases

BgeeiENSG00000151490
CleanExiHS_PTPRO
ExpressionAtlasiQ16827 baseline and differential
GenevisibleiQ16827 HS

Organism-specific databases

HPAiHPA034525

Interactioni

Subunit structurei

Interacts (phosphorylated form) with FYN and GRB2.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111764, 74 interactors
IntActiQ16827, 36 interactors
MINTiQ16827
STRINGi9606.ENSP00000281171

Structurei

Secondary structure

11216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi920 – 922Combined sources3
Helixi923 – 946Combined sources24
Turni947 – 951Combined sources5
Helixi956 – 958Combined sources3
Helixi960 – 965Combined sources6
Turni975 – 977Combined sources3
Beta strandi978 – 980Combined sources3
Turni988 – 991Combined sources4
Beta strandi992 – 998Combined sources7
Beta strandi1007 – 1010Combined sources4
Helixi1015 – 1017Combined sources3
Helixi1018 – 1027Combined sources10
Beta strandi1032 – 1035Combined sources4
Beta strandi1039 – 1041Combined sources3
Beta strandi1053 – 1056Combined sources4
Beta strandi1058 – 1060Combined sources3
Beta strandi1063 – 1072Combined sources10
Beta strandi1074 – 1085Combined sources12
Beta strandi1088 – 1097Combined sources10
Beta strandi1103 – 1105Combined sources3
Helixi1108 – 1127Combined sources20
Beta strandi1132 – 1140Combined sources9
Helixi1141 – 1158Combined sources18
Beta strandi1160 – 1162Combined sources3
Helixi1164 – 1172Combined sources9
Helixi1182 – 1202Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G59X-ray2.19A/B914-1200[»]
2GJTX-ray2.15A/B916-1208[»]
ProteinModelPortaliQ16827
SMRiQ16827
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ16827

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 124Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST75
Domaini142 – 211Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST70
Domaini246 – 306Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST61
Domaini329 – 425Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST97
Domaini435 – 531Fibronectin type-III 5PROSITE-ProRule annotationAdd BLAST97
Domaini532 – 628Fibronectin type-III 6PROSITE-ProRule annotationAdd BLAST97
Domaini631 – 724Fibronectin type-III 7PROSITE-ProRule annotationAdd BLAST94
Domaini725 – 817Fibronectin type-III 8PROSITE-ProRule annotationAdd BLAST93
Domaini938 – 1195Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST258

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1136 – 1142Substrate bindingBy similarity7

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0791 Eukaryota
COG5599 LUCA
GeneTreeiENSGT00900000140785
HOGENOMiHOG000115792
HOVERGENiHBG053763
InParanoidiQ16827
KOiK18035
OMAiSVTACTE
OrthoDBiEOG091G00JF
PhylomeDBiQ16827
TreeFamiTF351926

Family and domain databases

CDDicd00063 FN3, 3 hits
Gene3Di2.60.40.10, 3 hits
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
IPR029021 Prot-tyrosine_phosphatase-like
IPR000242 PTPase_domain
IPR016130 Tyr_Pase_AS
IPR003595 Tyr_Pase_cat
IPR000387 TYR_PHOSPHATASE_dom
PfamiView protein in Pfam
PF00041 fn3, 2 hits
PF00102 Y_phosphatase, 1 hit
PRINTSiPR00700 PRTYPHPHTASE
SMARTiView protein in SMART
SM00060 FN3, 4 hits
SM00194 PTPc, 1 hit
SM00404 PTPc_motif, 1 hit
SUPFAMiSSF49265 SSF49265, 2 hits
SSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS50853 FN3, 5 hits
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50055 TYR_PHOSPHATASE_PTP, 1 hit

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q16827-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGHLPTGIHG ARRLLPLLWL FVLFKNATAF HVTVQDDNNI VVSLEASDVI
60 70 80 90 100
SPASVYVVKI TGESKNYFFE FEEFNSTLPP PVIFKASYHG LYYIITLVVV
110 120 130 140 150
NGNVVTKPSR SITVLTKPLP VTSVSIYDYK PSPETGVLFE IHYPEKYNVF
160 170 180 190 200
TRVNISYWEG KDFRTMLYKD FFKGKTVFNH WLPGMCYSNI TFQLVSEATF
210 220 230 240 250
NKSTLVEYSG VSHEPKQHRT APYPPQNISV RIVNLNKNNW EEQSGNFPEE
260 270 280 290 300
SFMRSQDTIG KEKLFHFTEE TPEIPSGNIS SGWPDFNSSD YETTSQPYWW
310 320 330 340 350
DSASAAPESE DEFVSVLPME YENNSTLSET EKSTSGSFSF FPVQMILTWL
360 370 380 390 400
PPKPPTAFDG FHIHIEREEN FTEYLMVDEE AHEFVAELKE PGKYKLSVTT
410 420 430 440 450
FSSSGSCETR KSQSAKSLSF YISPSGEWIE ELTEKPQHVS VHVLSSTTAL
460 470 480 490 500
MSWTSSQENY NSTIVSVVSL TCQKQKESQR LEKQYCTQVN SSKPIIENLV
510 520 530 540 550
PGAQYQVVIY LRKGPLIGPP SDPVTFAIVP TGIKDLMLYP LGPTAVVLSW
560 570 580 590 600
TRPYLGVFRK YVVEMFYFNP ATMTSEWTTY YEIAATVSLT ASVRIANLLP
610 620 630 640 650
AWYYNFRVTM VTWGDPELSC CDSSTISFIT APVAPEITSV EYFNSLLYIS
660 670 680 690 700
WTYGDDTTDL SHSRMLHWMV VAEGKKKIKK SVTRNVMTAI LSLPPGDIYN
710 720 730 740 750
LSVTACTERG SNTSMLRLVK LEPAPPKSLF AVNKTQTSVT LLWVEEGVAD
760 770 780 790 800
FFEVFCQQVG SSQKTKLQEP VAVSSHVVTI SSLLPATAYN CSVTSFSHDS
810 820 830 840 850
PSVPTFIAVS TMVTEMNPNV VVISVLAILS TLLIGLLLVT LIILRKKHLQ
860 870 880 890 900
MARECGAGTF VNFASLERDG KLPYNWRRSI FAFLTLLPSC LWTDYLLAFY
910 920 930 940 950
INPWSKNGLK KRKLTNPVQL DDFDAYIKDM AKDSDYKFSL QFEELKLIGL
960 970 980 990 1000
DIPHFAADLP LNRCKNRYTN ILPYDFSRVR LVSMNEEEGA DYINANYIPG
1010 1020 1030 1040 1050
YNSPQEYIAT QGPLPETRND FWKMVLQQKS QIIVMLTQCN EKRRVKCDHY
1060 1070 1080 1090 1100
WPFTEEPIAY GDITVEMISE EEQDDWACRH FRINYADEMQ DVMHFNYTAW
1110 1120 1130 1140 1150
PDHGVPTANA AESILQFVHM VRQQATKSKG PMIIHCSAGV GRTGTFIALD
1160 1170 1180 1190 1200
RLLQHIRDHE FVDILGLVSE MRSYRMSMVQ TEEQYIFIHQ CVQLMWMKKK
1210
QQFCISDVIY ENVSKS
Length:1,216
Mass (Da):138,344
Last modified:October 14, 2008 - v2
Checksum:iC902B48D1A73BFE1
GO
Isoform 2 (identifier: Q16827-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     876-903: Missing.

Show »
Length:1,188
Mass (Da):134,941
Checksum:iC27FCA3BDFA0D1EF
GO
Isoform 3 (identifier: Q16827-3) [UniParc]FASTAAdd to basket
Also known as: PTPROt

The sequence of this isoform differs from the canonical sequence as follows:
     1-811: Missing.

Note: Predominantly expressed in B-lymphoid tissues.
Show »
Length:405
Mass (Da):47,163
Checksum:iD7BED5FFF7312782
GO
Isoform 4 (identifier: Q16827-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-811: Missing.
     876-903: Missing.

Show »
Length:377
Mass (Da):43,760
Checksum:i42E10120DBF2D1EF
GO
Isoform 5 (identifier: Q16827-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     594-597: RIAN → VIFP
     598-1216: Missing.

Note: No experimental confirmation available.
Show »
Length:597
Mass (Da):67,672
Checksum:iC0B2674199E893AA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti159E → G in CAA88425 (PubMed:7753550).Curated1
Sequence conflicti159E → G in AAA82892 (PubMed:7665166).Curated1
Sequence conflicti196S → C in CAA88425 (PubMed:7753550).Curated1
Sequence conflicti205L → V in CAA88425 (PubMed:7753550).Curated1
Sequence conflicti314V → F in CAA88425 (PubMed:7753550).Curated1
Sequence conflicti441V → F in CAA88425 (PubMed:7753550).Curated1
Sequence conflicti672A → T in CAA88425 (PubMed:7753550).Curated1
Sequence conflicti698I → T in CAA88425 (PubMed:7753550).Curated1
Sequence conflicti705A → T in CAA88425 (PubMed:7753550).Curated1
Sequence conflicti753E → K in CAA88425 (PubMed:7753550).Curated1
Sequence conflicti756 – 758CQQ → FQH in CAA88425 (PubMed:7753550).Curated3
Sequence conflicti775S → P in CAA88425 (PubMed:7753550).Curated1
Sequence conflicti790N → S in CAA88425 (PubMed:7753550).Curated1
Sequence conflicti861 – 863VNF → ANC in CAA88425 (PubMed:7753550).Curated3
Sequence conflicti876W → C in CAA88425 (PubMed:7753550).Curated1
Sequence conflicti876W → C in AAA82892 (PubMed:7665166).Curated1
Sequence conflicti896L → P in CAA88425 (PubMed:7753550).Curated1
Sequence conflicti896L → P in AAA82892 (PubMed:7665166).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0431361 – 811Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST811
Alternative sequenceiVSP_054481594 – 597RIAN → VIFP in isoform 5. 1 Publication4
Alternative sequenceiVSP_054482598 – 1216Missing in isoform 5. 1 PublicationAdd BLAST619
Alternative sequenceiVSP_035586876 – 903Missing in isoform 2 and isoform 4. 3 PublicationsAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48541 mRNA Translation: CAA88425.1
U20489 mRNA Translation: AAA82892.1
AF187043 mRNA Translation: AAF04086.1
AF187044 mRNA Translation: AAF04087.1
AK290982 mRNA Translation: BAF83671.1
AC007542 Genomic DNA No translation available.
AC022334 Genomic DNA No translation available.
AC092183 Genomic DNA No translation available.
CH471094 Genomic DNA Translation: EAW96349.1
CH471094 Genomic DNA Translation: EAW96350.1
CH471094 Genomic DNA Translation: EAW96351.1
BC035960 mRNA Translation: AAH35960.1
BC126201 mRNA Translation: AAI26202.1
BC126203 mRNA Translation: AAI26204.1
CCDSiCCDS44837.1 [Q16827-3]
CCDS53754.1 [Q16827-4]
CCDS8674.1 [Q16827-2]
CCDS8675.1 [Q16827-1]
PIRiA57064
S60613
RefSeqiNP_002839.1, NM_002848.3 [Q16827-2]
NP_109592.1, NM_030667.2 [Q16827-1]
NP_109593.1, NM_030668.2 [Q16827-4]
NP_109594.1, NM_030669.2 [Q16827-3]
NP_109595.1, NM_030670.2 [Q16827-4]
NP_109596.1, NM_030671.2 [Q16827-3]
UniGeneiHs.160871

Genome annotation databases

EnsembliENST00000281171; ENSP00000281171; ENSG00000151490 [Q16827-1]
ENST00000348962; ENSP00000343434; ENSG00000151490 [Q16827-2]
ENST00000442921; ENSP00000404188; ENSG00000151490 [Q16827-3]
ENST00000445537; ENSP00000393449; ENSG00000151490 [Q16827-3]
ENST00000542557; ENSP00000437571; ENSG00000151490 [Q16827-4]
ENST00000543886; ENSP00000444173; ENSG00000151490 [Q16827-5]
ENST00000544244; ENSP00000439234; ENSG00000151490 [Q16827-4]
GeneIDi5800
KEGGihsa:5800
UCSCiuc001rcu.3 human [Q16827-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPTPRO_HUMAN
AccessioniPrimary (citable) accession number: Q16827
Secondary accession number(s): A0AV39
, Q13101, Q8IYG3, Q9UBF0, Q9UBT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: October 14, 2008
Last modified: June 20, 2018
This is version 173 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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