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Reviewed, UniProtKB/Swiss-Prot Q16820 (MEP1B_HUMAN)

Last modified June 16, 2009. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Meprin A subunit beta
    EC=3.4.24.18
Alternative name(s):
    Endopeptidase-2
    N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta
    PABA peptide hydrolase
    PPH beta
Gene names
Name: MEP1B
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length701 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

Hydrolysis of protein and peptide substrates preferentially on carboxyl side of hydrophobic residues.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Homotetramer of alpha or beta subunits; heterotetramer of two alpha and two beta subunits are formed by non-covalent association of two disulfide-linked heterodimers By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Sequence similarities

Belongs to the peptidase M12A family.

Contains 1 EGF-like domain.

Contains 1 MAM domain.

Contains 1 MATH domain.

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Ontologies

Keywords
   Cellular componentMembrane
   Coding sequence diversityPolymorphism
   DomainEGF-like domain
Signal
Transmembrane
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processdigestion Ref.1

Traceable author statement. Source: ProtInc

proteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular space Ref.1

Traceable author statement. Source: ProtInc

integral to plasma membrane Ref.1

Traceable author statement. Source: ProtInc

   Molecular functionmetalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding Ref.1

Traceable author statement. Source: ProtInc

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Propeptide23 – 6139 Ref.1 Ref.3
PRO_0000028883
Chain62 – 701640Meprin A subunit beta
PRO_0000028884

Regions

Topological domain23 – 652630Extracellular Potential
Transmembrane653 – 67321 Potential
Topological domain674 – 70128Cytoplasmic Potential
Domain260 – 429170MAM
Domain430 – 585156MATH
Domain604 – 64441EGF-like
Region64 – 259196Metalloprotease

Sites

Active site1531 By similarity
Metal binding1521Zinc; catalytic By similarity
Metal binding1561Zinc; catalytic By similarity
Metal binding1621Zinc; catalytic By similarity

Amino acid modifications

Glycosylation2181N-linked (GlcNAc...) Potential
Glycosylation2541N-linked (GlcNAc...) Potential
Glycosylation3701N-linked (GlcNAc...) Potential
Glycosylation4211N-linked (GlcNAc...) Potential
Glycosylation4361N-linked (GlcNAc...) Potential
Glycosylation4451N-linked (GlcNAc...) Potential
Glycosylation5471N-linked (GlcNAc...) Potential
Glycosylation5921N-linked (GlcNAc...) Potential
Disulfide bond305Interchain (with C-308 in MEP1A) By similarity
Disulfide bond608 ↔ 619 By similarity
Disulfide bond613 ↔ 628 By similarity
Disulfide bond630 ↔ 643 By similarity

Natural variations

Natural variant3261V → M: dbSNP rs9959396.
VAR_057064
Natural variant6951P → L: dbSNP rs616114.
VAR_057065

Experimental info

Sequence conflict711W → S AA sequence Ref.3
Sequence conflict741T → P AA sequence Ref.3
Sequence conflict5461P → S in CAA57107. Ref.1
Sequence conflict6981Missing in CAA57107. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q16820-1 [UniParc].

Last modified November 23, 2004. Version 2.
Checksum: 473126DE05BB13FE

FASTA70179,581
        10         20         30         40         50         60 
MDLWNLSWFL FLDALLVISG LATPENFDVD GGMDQDIFDI NEGLGLDLFE GDIRLDRAQI 

        70         80         90        100        110        120 
RNSIIGEKYR WPHTIPYVLE DSLEMNAKGV ILNAFERYRL KTCIDFKPWA GETNYISVFK 

       130        140        150        160        170        180 
GSGCWSSVGN RRVGKQELSI GANCDRIATV QHEFLHALGF WHEQSRSDRD DYVRIMWDRI 

       190        200        210        220        230        240 
LSGREHNFNT YSDDISDSLN VPYDYTSVMH YSKTAFQNGT EPTIVTRISD FEDVIGQRMD 

       250        260        270        280        290        300 
FSDSDLLKLN QLYNCSSSLS FMDSCSFELE NVCGMIQSSG DNADWQRVSQ VPRGPESDHS 

       310        320        330        340        350        360 
NMGQCQGSGF FMHFDSSSVN VGATAVLESR TLYPKRGFQC LQFYLYNSGS ESDQLNIYIR 

       370        380        390        400        410        420 
EYSADNVDGN LTLVEEIKEI PTGSWQLYHV TLKVTKKFRV VFEGRKGSGA SLGGLSIDDI 

       430        440        450        460        470        480 
NLSETRCPHH IWHIRNFTQF IGSPNGTLYS PPFYSSKGYA FQIYLNLAHV TNAGIYFHLI 

       490        500        510        520        530        540 
SGANDDQLQW PCPWQQATMT LLDQNPDIRQ RMSNQRSITT DPFMTTDNGN YFWDRPSKVG 

       550        560        570        580        590        600 
TVALFPNGTQ FRRGGGYGTS AFITHERLKS RDFIKGDDVY ILLTVEDISH LNSTQIQLTP 

       610        620        630        640        650        660 
APSVQDLCSK TTCKNDGVCT VRDGKAECRC QSGEDWWYMG ERCEKRGSTR DTIVIAVSST 

       670        680        690        700 
VAVFALMLII TLVSVYCTRK KYRERMSSNR PNLTPQNQHA F

« Hide

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References

[1]"Polarised expression of human intestinal N-benzoyl-L-tyrosyl-p-aminobenzoic acid hydrolase (human meprin) alpha and beta subunits in Madin-Darby canine kidney cells."
Eldering J.A., Gruenberg J., Hahn D., Croes H.J., Fransen J.A., Sterchi E.E.
Eur. J. Biochem. 247:920-932(1997) [PubMed: 9288916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 62-105, VARIANT LEU-695.
Tissue: Intestine.
[2]"Expression of human meprin beta."
Haun R.S.
Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Small intestine.
[3]"Cloning of the PABA peptide hydrolase alpha subunit (PPH alpha) from human small intestine and its expression in COS-1 cells."
Dumermuth E., Eldering J.A., Gruenberg J., Jiang W., Sterchi E.E.
FEBS Lett. 335:367-375(1993) [PubMed: 8262185] [Abstract]
Cited for: PROTEIN SEQUENCE OF 62-79.
Tissue: Small intestine mucosa.
+Additional computationally mapped references.

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Cross-references

Sequence databases

X81333 mRNA. Translation: CAA57107.1.
AY695931 mRNA. Translation: AAU05377.1.
IPIIPI00178015.
PIRHYHUMB. S49383.
RefSeqNP_005916.2.
UniGeneHs.194777

3D structure databases

HSSPHSSP built from PDB template 1IAE based on UniProtKB P07584.
ModBaseSearch...

Protein family/group databases

MEROPSM12.004.

Proteomic databases

PRIDEQ16820.

Genome annotation databases

EnsemblENSG00000141434. Homo sapiens. [Contig view]
GeneID4225.
KEGGhsa:4225.

Organism-specific databases

GeneCardsGC18P028023.
H-InvDBHIX0039722.
HGNCHGNC:7020. MEP1B.
MIM600389. gene.
PharmGKBPA30754.
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ16820.
HOVERGENQ16820.

Enzyme and pathway databases

BRENDA3.4.24.18. 247.

Gene expression databases

ArrayExpressQ16820.
BgeeQ16820.
CleanExHS_MEP1B.
GermOnlineENSG00000141434. Homo sapiens.

Family and domain databases

InterProIPR006210. EGF-like.
IPR013032. EGF-like_reg_CS.
IPR000742. EGF_3.
IPR000998. MAM.
IPR002083. MATH.
IPR008294. Pept_M12A_Meprin.
IPR006025. Pept_M_Zn_BS.
IPR006026. Peptidase_M.
IPR001506. Peptidase_M12A.
IPR013322. TRAF-type.
[Graphical view]
Gene3DG3DSA:2.60.210.10. TRAF-type. 1 hit.
PfamPF01400. Astacin. 1 hit.
PF00629. MAM. 1 hit.
PF00917. MATH. 1 hit.
[Graphical view]
PIRSFPIRSF001196. Meprin. 1 hit.
PRINTSPR00480. ASTACIN.
PR00020. MAMDOMAIN.
SMARTSM00181. EGF. 1 hit.
SM00137. MAM. 1 hit.
SM00061. MATH. 1 hit.
SM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEPS00022. EGF_1. False negative.
PS01186. EGF_2. False negative.
PS50026. EGF_3. 1 hit.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS50144. MATH. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio16675.
SOURCESearch...

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Entry information

Entry nameMEP1B_HUMAN
AccessionPrimary (citable) accession number: Q16820
Secondary accession number(s): Q670J1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 23, 2004
Last modified: June 16, 2009
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 18

Human chromosome 18: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents