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Q16820 (MEP1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Meprin A subunit beta
EC=3.4.24.63
Alternative name(s):
Endopeptidase-2
Meprin B
N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta
PABA peptide hydrolase
PPH beta
Gene names
Name:MEP1B
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length701 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of proteins, including azocasein, and peptides. Hydrolysis of 5-His-|-Leu-6, 6-Leu-|-Cys-7, 14-Ala-|-Leu-15 and 19-Cys-|-Gly-20 bonds in insulin B chain.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Homotetramer consisting of disulfide-linked beta subunits, or heterotetramer of two alpha and two beta subunits formed by non-covalent association of two disulfide-linked heterodimers By similarity. Interacts with MBL2 through its carbohydrate moiety. This interaction may inhibit its catalytic activity By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Post-translational modification

N-glycosylated; contains high mannoase and/or complex biantennary structures By similarity.

Sequence similarities

Belongs to the peptidase M12A family.

Contains 1 EGF-like domain.

Contains 1 MAM domain.

Contains 1 MATH domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Propeptide23 – 6139
PRO_0000028883
Chain62 – 701640Meprin A subunit beta
PRO_0000028884

Regions

Topological domain23 – 652630Extracellular Potential
Transmembrane653 – 67321Helical; Potential
Topological domain674 – 70128Cytoplasmic Potential
Domain260 – 429170MAM
Domain430 – 585156MATH
Domain604 – 64441EGF-like
Region64 – 259196Metalloprotease

Sites

Active site1531 By similarity
Metal binding1521Zinc; catalytic By similarity
Metal binding1561Zinc; catalytic By similarity
Metal binding1621Zinc; catalytic By similarity

Amino acid modifications

Glycosylation2181N-linked (GlcNAc...) Potential
Glycosylation2541N-linked (GlcNAc...) Potential
Glycosylation3701N-linked (GlcNAc...) Potential
Glycosylation4211N-linked (GlcNAc...) Potential
Glycosylation4361N-linked (GlcNAc...) Potential
Glycosylation4451N-linked (GlcNAc...) Potential
Glycosylation5471N-linked (GlcNAc...) Potential
Glycosylation5921N-linked (GlcNAc...) Potential
Disulfide bond103 ↔ 255 By similarity
Disulfide bond124 ↔ 144 By similarity
Disulfide bond265 ↔ 427 By similarity
Disulfide bond273Interchain By similarity
Disulfide bond305Interchain By similarity
Disulfide bond492Interchain By similarity
Disulfide bond608 ↔ 619 By similarity
Disulfide bond613 ↔ 628 By similarity
Disulfide bond630 ↔ 643 By similarity

Natural variations

Natural variant3261V → M.
Corresponds to variant rs9959396 [ dbSNP | Ensembl ].
VAR_057064
Natural variant6951P → L. Ref.1 Ref.3
Corresponds to variant rs616114 [ dbSNP | Ensembl ].
VAR_057065

Experimental info

Sequence conflict711W → S AA sequence Ref.4
Sequence conflict741T → P AA sequence Ref.4
Sequence conflict5461S → P in AAU05377. Ref.2
Sequence conflict5461S → P in AAI36560. Ref.3
Sequence conflict5461S → P in AAI44245. Ref.3
Sequence conflict6981Missing in CAA57107. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q16820 [UniParc].

Last modified May 18, 2010. Version 3.
Checksum: 4733DBF018A4F3CD

FASTA70179,571
        10         20         30         40         50         60 
MDLWNLSWFL FLDALLVISG LATPENFDVD GGMDQDIFDI NEGLGLDLFE GDIRLDRAQI 

        70         80         90        100        110        120 
RNSIIGEKYR WPHTIPYVLE DSLEMNAKGV ILNAFERYRL KTCIDFKPWA GETNYISVFK 

       130        140        150        160        170        180 
GSGCWSSVGN RRVGKQELSI GANCDRIATV QHEFLHALGF WHEQSRSDRD DYVRIMWDRI 

       190        200        210        220        230        240 
LSGREHNFNT YSDDISDSLN VPYDYTSVMH YSKTAFQNGT EPTIVTRISD FEDVIGQRMD 

       250        260        270        280        290        300 
FSDSDLLKLN QLYNCSSSLS FMDSCSFELE NVCGMIQSSG DNADWQRVSQ VPRGPESDHS 

       310        320        330        340        350        360 
NMGQCQGSGF FMHFDSSSVN VGATAVLESR TLYPKRGFQC LQFYLYNSGS ESDQLNIYIR 

       370        380        390        400        410        420 
EYSADNVDGN LTLVEEIKEI PTGSWQLYHV TLKVTKKFRV VFEGRKGSGA SLGGLSIDDI 

       430        440        450        460        470        480 
NLSETRCPHH IWHIRNFTQF IGSPNGTLYS PPFYSSKGYA FQIYLNLAHV TNAGIYFHLI 

       490        500        510        520        530        540 
SGANDDQLQW PCPWQQATMT LLDQNPDIRQ RMSNQRSITT DPFMTTDNGN YFWDRPSKVG 

       550        560        570        580        590        600 
TVALFSNGTQ FRRGGGYGTS AFITHERLKS RDFIKGDDVY ILLTVEDISH LNSTQIQLTP 

       610        620        630        640        650        660 
APSVQDLCSK TTCKNDGVCT VRDGKAECRC QSGEDWWYMG ERCEKRGSTR DTIVIAVSST 

       670        680        690        700 
VAVFALMLII TLVSVYCTRK KYRERMSSNR PNLTPQNQHA F

« Hide

References

« Hide 'large scale' references
[1]"Polarised expression of human intestinal N-benzoyl-L-tyrosyl-p-aminobenzoic acid hydrolase (human meprin) alpha and beta subunits in Madin-Darby canine kidney cells."
Eldering J.A., Gruenberg J., Hahn D., Croes H.J., Fransen J.A., Sterchi E.E.
Eur. J. Biochem. 247:920-932(1997) [PubMed: 9288916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 62-105, VARIANT LEU-695.
Tissue: Intestine.
[2]"Expression of human meprin beta."
Haun R.S.
Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Small intestine.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-695.
Tissue: Testis.
[4]"Cloning of the PABA peptide hydrolase alpha subunit (PPH alpha) from human small intestine and its expression in COS-1 cells."
Dumermuth E., Eldering J.A., Gruenberg J., Jiang W., Sterchi E.E.
FEBS Lett. 335:367-375(1993) [PubMed: 8262185] [Abstract]
Cited for: PROTEIN SEQUENCE OF 62-79.
Tissue: Small intestine mucosa.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X81333 mRNA. Translation: CAA57107.1.
AY695931 mRNA. Translation: AAU05377.1.
BC136559 mRNA. Translation: AAI36560.1.
BC144244 mRNA. Translation: AAI44245.1.
IPIIPI00178015.
PIRHYHUMB. S49383.
RefSeqNP_005916.2. NM_005925.2.
UniGeneHs.194777.

3D structure databases

ProteinModelPortalQ16820.
SMRQ16820. Positions 65-255, 260-586, 606-648.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ16820.

Protein family/group databases

MEROPSM12.004.

PTM databases

PhosphoSiteQ16820.

Polymorphism databases

DMDM296439304.

Proteomic databases

PRIDEQ16820.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000269202; ENSP00000269202; ENSG00000141434.
GeneID4225.
KEGGhsa:4225.
UCSCuc002kxj.2. human.

Organism-specific databases

CTD4225.
GeneCardsGC18P029793.
H-InvDBHIX0039722.
HGNCHGNC:7020. MEP1B.
HPACAB025850.
MIM600389. gene.
neXtProtNX_Q16820.
PharmGKBPA30754.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG05906.
GeneTreeENSGT00550000074269.
HOGENOMHBG445172.
HOVERGENHBG052457.
InParanoidQ16820.
OMAWQRVSQV.
OrthoDBEOG408N7Q.
PhylomeDBQ16820.

Gene expression databases

ArrayExpressQ16820.
BgeeQ16820.
CleanExHS_MEP1B.
GenevestigatorQ16820.
GermOnlineENSG00000141434. Homo sapiens.

Family and domain databases

InterProIPR008985. ConA-like_lec_gl.
IPR006210. EGF-like.
IPR000742. EGF_3.
IPR000998. MAM_dom.
IPR002083. MATH.
IPR024079. MetalloPept_cat_dom.
IPR008294. Pept_M12A_Meprin.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
IPR008974. TRAF-like.
IPR013322. TRAF-type.
[Graphical view]
Gene3DG3DSA:3.40.390.10. G3DSA:3.40.390.10. 1 hit.
G3DSA:2.60.210.10. TRAF-type. 1 hit.
KOK08606.
PfamPF01400. Astacin. 1 hit.
PF00629. MAM. 1 hit.
PF00917. MATH. 1 hit.
[Graphical view]
PIRSFPIRSF001196. Meprin. 1 hit.
PRINTSPR00480. ASTACIN.
PR00020. MAMDOMAIN.
SMARTSM00181. EGF. 1 hit.
SM00137. MAM. 1 hit.
SM00061. MATH. 1 hit.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMSSF49899. ConA_like_lec_gl. 1 hit.
SSF49599. Traf_like. 1 hit.
PROSITEPS00022. EGF_1. False negative.
PS01186. EGF_2. False negative.
PS50026. EGF_3. 1 hit.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS50144. MATH. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio16675.
SOURCESearch...

Entry information

Entry nameMEP1B_HUMAN
AccessionPrimary (citable) accession number: Q16820
Secondary accession number(s): B7ZM35, B9EGL6, Q670J1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 18, 2010
Last modified: January 25, 2012
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

Human chromosome 18

Human chromosome 18: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents