Q16820 (MEP1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 115.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Meprin A subunit beta EC=3.4.24.63 Alternative name(s): Endopeptidase-2 Meprin B N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta PABA peptide hydrolase PPH beta | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 701 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | Hydrolysis of proteins, including azocasein, and peptides. Hydrolysis of 5-His-|-Leu-6, 6-Leu-|-Cys-7, 14-Ala-|-Leu-15 and 19-Cys-|-Gly-20 bonds in insulin B chain. |
| Cofactor | Binds 1 zinc ion per subunit By similarity. |
| Subunit structure | Homotetramer consisting of disulfide-linked beta subunits, or heterotetramer of two alpha and two beta subunits formed by non-covalent association of two disulfide-linked heterodimers By similarity. Interacts with MBL2 through its carbohydrate moiety. This interaction may inhibit its catalytic activity By similarity. |
| Subcellular location | |
| Post-translational modification | N-glycosylated; contains high mannoase and/or complex biantennary structures By similarity. |
| Sequence similarities | Belongs to the peptidase M12A family. Contains 1 EGF-like domain. Contains 1 MAM domain. Contains 1 MATH domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Membrane |
| Coding sequence diversity | Polymorphism |
| Domain | EGF-like domain Signal Transmembrane Transmembrane helix |
| Ligand | Metal-binding Zinc |
| Molecular function | Hydrolase Metalloprotease Protease |
| PTM | Disulfide bond Glycoprotein Zymogen |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological process | digestion Traceable author statement. Source: ProtInc proteolysisInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | extracellular space Traceable author statement. Source: ProtInc integral to plasma membraneTraceable author statement. Source: ProtInc |
| Molecular function | metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion bindingTraceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 22 | 22 | Potential | ||||||||
| Propeptide | 23 – 61 | 39 | PRO_0000028883 | ||||||||
| Chain | 62 – 701 | 640 | Meprin A subunit beta | PRO_0000028884 | |||||||
Regions | |||||||||||
| Topological domain | 23 – 652 | 630 | Extracellular Potential | ||||||||
| Transmembrane | 653 – 673 | 21 | Helical; Potential | ||||||||
| Topological domain | 674 – 701 | 28 | Cytoplasmic Potential | ||||||||
| Domain | 260 – 429 | 170 | MAM | ||||||||
| Domain | 430 – 585 | 156 | MATH | ||||||||
| Domain | 604 – 644 | 41 | EGF-like | ||||||||
| Region | 64 – 259 | 196 | Metalloprotease | ||||||||
Sites | |||||||||||
| Active site | 153 | 1 | By similarity | ||||||||
| Metal binding | 152 | 1 | Zinc; catalytic By similarity | ||||||||
| Metal binding | 156 | 1 | Zinc; catalytic By similarity | ||||||||
| Metal binding | 162 | 1 | Zinc; catalytic By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 218 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 254 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 370 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 421 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 436 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 445 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 547 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 592 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 103 ↔ 255 | By similarity | |||||||||
| Disulfide bond | 124 ↔ 144 | By similarity | |||||||||
| Disulfide bond | 265 ↔ 427 | By similarity | |||||||||
| Disulfide bond | 273 | Interchain By similarity | |||||||||
| Disulfide bond | 305 | Interchain By similarity | |||||||||
| Disulfide bond | 492 | Interchain By similarity | |||||||||
| Disulfide bond | 608 ↔ 619 | By similarity | |||||||||
| Disulfide bond | 613 ↔ 628 | By similarity | |||||||||
| Disulfide bond | 630 ↔ 643 | By similarity | |||||||||
Natural variations | |||||||||||
| Natural variant | 326 | 1 | V → M. Corresponds to variant rs9959396 [ dbSNP | Ensembl ]. | VAR_057064 | |||||||
| Natural variant | 695 | 1 | P → L. Ref.1 Ref.3 Corresponds to variant rs616114 [ dbSNP | Ensembl ]. | VAR_057065 | |||||||
Experimental info | |||||||||||
| Sequence conflict | 71 | 1 | W → S AA sequence Ref.4 | ||||||||
| Sequence conflict | 74 | 1 | T → P AA sequence Ref.4 | ||||||||
| Sequence conflict | 546 | 1 | S → P in AAU05377. Ref.2 | ||||||||
| Sequence conflict | 546 | 1 | S → P in AAI36560. Ref.3 | ||||||||
| Sequence conflict | 546 | 1 | S → P in AAI44245. Ref.3 | ||||||||
| Sequence conflict | 698 | 1 | Missing in CAA57107. Ref.1 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Polarised expression of human intestinal N-benzoyl-L-tyrosyl-p-aminobenzoic acid hydrolase (human meprin) alpha and beta subunits in Madin-Darby canine kidney cells." Eldering J.A., Gruenberg J., Hahn D., Croes H.J., Fransen J.A., Sterchi E.E. Eur. J. Biochem. 247:920-932(1997) [PubMed: 9288916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 62-105, VARIANT LEU-695. Tissue: Intestine. |
| [2] | "Expression of human meprin beta." Haun R.S. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Small intestine. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-695. Tissue: Testis. |
| [4] | "Cloning of the PABA peptide hydrolase alpha subunit (PPH alpha) from human small intestine and its expression in COS-1 cells." Dumermuth E., Eldering J.A., Gruenberg J., Jiang W., Sterchi E.E. FEBS Lett. 335:367-375(1993) [PubMed: 8262185] [Abstract] Cited for: PROTEIN SEQUENCE OF 62-79. Tissue: Small intestine mucosa. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X81333 mRNA. Translation: CAA57107.1. AY695931 mRNA. Translation: AAU05377.1. BC136559 mRNA. Translation: AAI36560.1. BC144244 mRNA. Translation: AAI44245.1. |
| IPI | IPI00178015. |
| PIR | HYHUMB. S49383. |
| RefSeq | NP_005916.2. NM_005925.2. |
| UniGene | Hs.194777. |
3D structure databases | |
| ProteinModelPortal | Q16820. |
| SMR | Q16820. Positions 65-255, 260-586, 606-648. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q16820. |
Protein family/group databases | |
| MEROPS | M12.004. |
PTM databases | |
| PhosphoSite | Q16820. |
Polymorphism databases | |
| DMDM | 296439304. |
Proteomic databases | |
| PRIDE | Q16820. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000269202; ENSP00000269202; ENSG00000141434. |
| GeneID | 4225. |
| KEGG | hsa:4225. |
| UCSC | uc002kxj.2. human. |
Organism-specific databases | |
| CTD | 4225. |
| GeneCards | GC18P029793. |
| H-InvDB | HIX0039722. |
| HGNC | HGNC:7020. MEP1B. |
| HPA | CAB025850. |
| MIM | 600389. gene. |
| neXtProt | NX_Q16820. |
| PharmGKB | PA30754. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG05906. |
| GeneTree | ENSGT00550000074269. |
| HOGENOM | HBG445172. |
| HOVERGEN | HBG052457. |
| InParanoid | Q16820. |
| OMA | WQRVSQV. |
| OrthoDB | EOG408N7Q. |
| PhylomeDB | Q16820. |
Gene expression databases | |
| ArrayExpress | Q16820. |
| Bgee | Q16820. |
| CleanEx | HS_MEP1B. |
| Genevestigator | Q16820. |
| GermOnline | ENSG00000141434. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR008985. ConA-like_lec_gl. IPR006210. EGF-like. IPR000742. EGF_3. IPR000998. MAM_dom. IPR002083. MATH. IPR024079. MetalloPept_cat_dom. IPR008294. Pept_M12A_Meprin. IPR001506. Peptidase_M12A. IPR006026. Peptidase_Metallo. IPR008974. TRAF-like. IPR013322. TRAF-type. [Graphical view] |
| Gene3D | G3DSA:3.40.390.10. G3DSA:3.40.390.10. 1 hit. G3DSA:2.60.210.10. TRAF-type. 1 hit. |
| KO | K08606. |
| Pfam | PF01400. Astacin. 1 hit. PF00629. MAM. 1 hit. PF00917. MATH. 1 hit. [Graphical view] |
| PIRSF | PIRSF001196. Meprin. 1 hit. |
| PRINTS | PR00480. ASTACIN. PR00020. MAMDOMAIN. |
| SMART | SM00181. EGF. 1 hit. SM00137. MAM. 1 hit. SM00061. MATH. 1 hit. SM00235. ZnMc. 1 hit. [Graphical view] |
| SUPFAM | SSF49899. ConA_like_lec_gl. 1 hit. SSF49599. Traf_like. 1 hit. |
| PROSITE | PS00022. EGF_1. False negative. PS01186. EGF_2. False negative. PS50026. EGF_3. 1 hit. PS00740. MAM_1. 1 hit. PS50060. MAM_2. 1 hit. PS50144. MATH. 1 hit. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 16675. |
| SOURCE | Search... |
Entry information
| Entry name | MEP1B_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q16820 Secondary accession number(s): B7ZM35, B9EGL6, Q670J1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| Human chromosome 18 Human chromosome 18: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with