Q167Q3 (KATG_ROSDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	RD1_2195

Organism

Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans) [Complete proteome] [HAMAP]

Taxonomic identifier

375451 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Roseobacter

Protein attributes

Sequence length	726 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 726	726	Catalase-peroxidase HAMAP MF_01961		PRO_0000354898
Sites
	Active site	99	1	Proton acceptor By similarity
	Metal binding	267	1	Iron (heme axial ligand) By similarity
	Site	95	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	98 ↔ 226		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-252) By similarity
	Cross-link	226 ↔ 252		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q167Q3 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: BC7555153FCC07D7	FASTA	726	79,400
	10 20 30 40 50 60 MDGNNVDPTG GCPVMHGGNT AMDKPVTKWW PNALNLDILH QHGARTNPMD PDYDHRAAVK 70 80 90 100 110 120 ALDFEAVKAD VKALMTQDQD WWPADWGHYG GLMIRLAWHS AGTYRMQDGR GGAGSGNIRF 130 140 150 160 170 180 APLNSWPDNA SLDKARRLLW PVKKKYGNAL SWADLIILSG NMAYESMGLK TFGFGFGRED 190 200 210 220 230 240 IWGPETDVYW GSENEWLAPS ENRYGDLDDA STLENPLAAV HMGLIYVNPE GVNGQPDPAR 250 260 270 280 290 300 TAQHVRETFA RMAMDDEETA ALTCGGHTVG KAHGRGAVDN IGVEPEAAGI EAQGFGWSNP 310 320 330 340 350 360 RHDGKATNAF TSGIEGAWTT HPTQWDMGYF KLLFGYEWQL TKSPAGAWQW EPIDIKEEDM 370 380 390 400 410 420 PVDPTDPSKR HQPMMTDADM AMKVDPIYNE ICQKFMADPE YFADVFGRAW FKLTHRDMGP 430 440 450 460 470 480 KACYIGPDVP AEDLIWQDPI PAGSTEYDVA AVKARIADSG LSAADMIATA WDSARTFRGS 490 500 510 520 530 540 DKRGGANGAR IRLAPQKDWA GNEPERLAKV LGILEPIAAE TGASVADVIV LAGNVGLEQS 550 560 570 580 590 600 IKAAGYDVDV PFAQGRGDAT ADQTDAASFD VLEPLADGFR NWQKADYAVS AEEMMLDKAQ 610 620 630 640 650 660 LLGLTAAEMT VLVGGMRVLG VNHGNSKHGV FTDRAGQLTP DFFVNLTDMA YSWHPVDGEN 670 680 690 700 710 720 TYEIRDRATG AVKWTATSAD LVFGSNSVLR AYAEVYAQDD NAEKFVRDFV AAWTKVMNAD RFDLAA

References

[1]

"The complete genome sequence of Roseobacter denitrificans reveals a mixotrophic rather than photosynthetic metabolism." Swingley W.D., Sadekar S., Mastrian S.D., Matthies H.J., Hao J., Ramos H., Acharya C.R., Conrad A.L., Taylor H.L., Dejesa L.C., Shah M.K., O'Huallachain M.E., Lince M.T., Blankenship R.E., Beatty J.T., Touchman J.W. J. Bacteriol. 189:683-690(2007) [PubMed: 17098896] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33942 / OCh 114.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000362 Genomic DNA. Translation: ABG31790.1.
RefSeq	YP_682476.1. NC_008209.1.
3D structure databases
ProteinModelPortal	Q167Q3.
SMR	Q167Q3. Positions 28-724.
ModBase	Search...
Protein-protein interaction databases
STRING	Q167Q3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4196542.
GenomeReviews	Gene locus RD1_2195 in contig CP000362_GR.
KEGG	rde:RD1_2195.
NMPDR	fig|375451.6.peg.2049.
PATRIC	23362433. VBIRosDen86677_2102.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	WPNALNL.
PhylomeDB	Q167Q3.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	RDEN375451:RD1_2195-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_ROSDO

Accession

Primary (citable) accession number: Q167Q3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	July 25, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program