ID   MAON_HUMAN              Reviewed;         604 AA.
AC   Q16798;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 2.
DT   07-JUL-2009, entry version 83.
DE   RecName: Full=NADP-dependent malic enzyme, mitochondrial;
DE            Short=NADP-ME;
DE            EC=1.1.1.40;
DE   AltName: Full=Malic enzyme 3;
DE   Flags: Precursor;
GN   Name=ME3;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT ASN-324.
RC   TISSUE=Hippocampus;
RX   MEDLINE=95118281; PubMed=7818469;
RA   Loeber G., Maurer-Fogy I., Schwendenwein R.;
RT   "Purification, cDNA cloning and heterologous expression of the human
RT   mitochondrial NADP(+)-dependent malic enzyme.";
RL   Biochem. J. 304:687-692(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16554811; DOI=10.1038/nature04632;
RA   Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA   Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F.,
RA   Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E.,
RA   FitzGerald M.G., Jaffe D.B., LaButti K., Nicol R., Park H.-S.,
RA   Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W.,
RA   Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S.,
RA   Sakaki Y.;
RT   "Human chromosome 11 DNA sequence and analysis including novel gene
RT   identification.";
RL   Nature 440:497-500(2006).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NADP(+) = pyruvate + CO(2) +
CC       NADPH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC   -!- TISSUE SPECIFICITY: Expressed predominantly in organs with a low-
CC       division rate.
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; X79440; CAA55956.1; -; mRNA.
DR   EMBL; AP001148; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP001831; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP002492; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   IPI; IPI00003970; -.
DR   PIR; S53351; S53351.
DR   RefSeq; NP_001014811.1; -.
DR   RefSeq; NP_006671.2; -.
DR   UniGene; Hs.199743; -.
DR   HSSP; P40927; 1GQ2.
DR   PRIDE; Q16798; -.
DR   Ensembl; ENSG00000151376; Homo sapiens.
DR   GeneID; 10873; -.
DR   KEGG; hsa:10873; -.
DR   UCSC; uc001pbz.1; human.
DR   GeneCards; GC11M085829; -.
DR   H-InvDB; HIX0010004; -.
DR   HGNC; HGNC:6985; ME3.
DR   MIM; 604626; gene.
DR   PharmGKB; PA30725; -.
DR   HOGENOM; Q16798; -.
DR   HOVERGEN; Q16798; -.
DR   OMA; Q16798; HEKEMFA.
DR   BRENDA; 1.1.1.40; 247.
DR   DrugBank; DB00157; NADH.
DR   NextBio; 41285; -.
DR   ArrayExpress; Q16798; -.
DR   Bgee; Q16798; -.
DR   CleanEx; HS_ME3; -.
DR   GermOnline; ENSG00000151376; Homo sapiens.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0004473; F:malate dehydrogenase (oxaloacetate-decarbox...; IDA:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0009060; P:aerobic respiration; TAS:UniProtKB.
DR   GO; GO:0006108; P:malate metabolic process; IDA:UniProtKB.
DR   GO; GO:0055114; P:oxidation reduction; IDA:UniProtKB.
DR   GO; GO:0006800; P:oxygen and reactive oxygen species metaboli...; TAS:UniProtKB.
DR   GO; GO:0006090; P:pyruvate metabolic process; IDA:UniProtKB.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   2: Evidence at transcript level;
KW   Complete proteome; Metal-binding; Mitochondrion; NADP; Oxidoreductase;
KW   Polymorphism; Transit peptide.
FT   TRANSIT       1      ?       Mitochondrion (Potential).
FT   CHAIN         ?    604       NADP-dependent malic enzyme,
FT                                mitochondrial.
FT                                /FTId=PRO_0000018539.
FT   ACT_SITE    137    137       Proton donor (By similarity).
FT   ACT_SITE    208    208       Proton acceptor (By similarity).
FT   METAL       280    280       Divalent metal cation (By similarity).
FT   METAL       281    281       Divalent metal cation (By similarity).
FT   METAL       304    304       Divalent metal cation (By similarity).
FT   BINDING     190    190       NAD (By similarity).
FT   BINDING     304    304       NAD (By similarity).
FT   BINDING     443    443       NAD (By similarity).
FT   SITE        304    304       Important for activity (By similarity).
FT   VARIANT      85     85       S -> G (in dbSNP:rs17856661).
FT                                /FTId=VAR_047369.
FT   VARIANT     324    324       K -> N (in dbSNP:rs1042780).
FT                                /FTId=VAR_047370.
SQ   SEQUENCE   604 AA;  67068 MW;  E0E79A75F3CAC524 CRC64;
     MGAALGTGTR LAPWPGRACG ALPRWTPTAP AQGCHSKPGP ARPVPLKKRG YDVTRNPHLN
     KGMAFTLEER LQLGIHGLIP PCFLSQDVQL LRIMRYYERQ QSDLDKYIIL MTLQDRNEKL
     FYRVLTSDVE KFMPIVYTPT VGLACQHYGL TFRRPRGLFI TIHDKGHLAT MLNSWPEDNI
     KAVVVTDGER ILGLGDLGCY GMGIPVGKLA LYTACGGVNP QQCLPVLLDV GTNNEELLRD
     PLYIGLKHQR VHGKAYDDLL DEFMQAVTDK FGINCLIQFE DFANANAFRL LNKYRNKYCM
     FNDDIQGTAS VAVAGILAAL RITKNKLSNH VFVFQGAGEA AMGIAHLLVM ALEKEGVPKA
     EATRKIWMVD SKGLIVKGRS HLNHEKEMFA QDHPEVNSLE EVVRLVKPTA IIGVAAIAGA
     FTEQILRDMA SFHERPIIFA LSNPTSKAEC TAEKCYRVTE GRGIFASGSP FKSVTLEDGK
     TFIPGQGNNA YVFPGVALGV IAGGIRHIPD EIFLLTAEQI AQEVSEQHLS QGRLYPPLST
     IRDVSLRIAI KVLDYAYKHN LASYYPEPKD KEAFVRSLVY TPDYDSFTLD SYTWPKEAMN
     VQTV
//