NADP-dependent malic enzyme, mitochondrial precursor

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Q16798 (MAON_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 120. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NADP-dependent malic enzyme, mitochondrial
Short name=NADP-ME
EC=1.1.1.40

Alternative name(s):
Malic enzyme 3

Gene names

Name:

ME3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	604 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	(S)-malate + NADP⁺ = pyruvate + CO₂ + NADPH. Oxaloacetate = pyruvate + CO₂.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subcellular location	Mitochondrion matrix.
Tissue specificity	Expressed predominantly in organs with a low-division rate.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Polymorphism
Domain	Transit peptide
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aerobic respiration Traceable author statement Ref.1. Source: UniProtKB malate metabolic process Inferred from direct assay Ref.1. Source: UniProtKB oxygen metabolic process Traceable author statement Ref.1. Source: UniProtKB pyruvate metabolic process Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Traceable author statement Ref.1. Source: UniProtKB
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro cofactor binding Traceable author statement Ref.1. Source: UniProtKB malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity Inferred from direct assay Ref.1. Source: UniProtKB malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Mitochondrion Potential

Chain

? – 604

NADP-dependent malic enzyme, mitochondrial

PRO_0000018539

Sites

Active site

137

Proton donor By similarity

Active site

208

Proton acceptor By similarity

Metal binding

280

Divalent metal cation By similarity

Metal binding

281

Divalent metal cation By similarity

Metal binding

304

Divalent metal cation By similarity

Binding site

190

NAD By similarity

Binding site

304

NAD By similarity

Binding site

443

NAD By similarity

Site

304

Important for activity By similarity

Natural variations

Natural variant

S → G.
Corresponds to variant rs17856661 [ dbSNP | Ensembl ].

VAR_047369

Natural variant

324

K → N. Ref.1
Corresponds to variant rs1042780 [ dbSNP | Ensembl ].

VAR_047370

Sequences

Sequence

Length

Mass (Da)

Tools

Q16798 [UniParc].

Last modified November 25, 2008. Version 2.
Checksum: E0E79A75F3CAC524
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FASTA

604

67,068

        10         20         30         40         50         60 
MGAALGTGTR LAPWPGRACG ALPRWTPTAP AQGCHSKPGP ARPVPLKKRG YDVTRNPHLN 

        70         80         90        100        110        120 
KGMAFTLEER LQLGIHGLIP PCFLSQDVQL LRIMRYYERQ QSDLDKYIIL MTLQDRNEKL 

       130        140        150        160        170        180 
FYRVLTSDVE KFMPIVYTPT VGLACQHYGL TFRRPRGLFI TIHDKGHLAT MLNSWPEDNI 

       190        200        210        220        230        240 
KAVVVTDGER ILGLGDLGCY GMGIPVGKLA LYTACGGVNP QQCLPVLLDV GTNNEELLRD 

       250        260        270        280        290        300 
PLYIGLKHQR VHGKAYDDLL DEFMQAVTDK FGINCLIQFE DFANANAFRL LNKYRNKYCM 

       310        320        330        340        350        360 
FNDDIQGTAS VAVAGILAAL RITKNKLSNH VFVFQGAGEA AMGIAHLLVM ALEKEGVPKA 

       370        380        390        400        410        420 
EATRKIWMVD SKGLIVKGRS HLNHEKEMFA QDHPEVNSLE EVVRLVKPTA IIGVAAIAGA 

       430        440        450        460        470        480 
FTEQILRDMA SFHERPIIFA LSNPTSKAEC TAEKCYRVTE GRGIFASGSP FKSVTLEDGK 

       490        500        510        520        530        540 
TFIPGQGNNA YVFPGVALGV IAGGIRHIPD EIFLLTAEQI AQEVSEQHLS QGRLYPPLST 

       550        560        570        580        590        600 
IRDVSLRIAI KVLDYAYKHN LASYYPEPKD KEAFVRSLVY TPDYDSFTLD SYTWPKEAMN 


VQTV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification, cDNA cloning and heterologous expression of the human mitochondrial NADP(+)-dependent malic enzyme." Loeber G., Maurer-Fogy I., Schwendenwein R. Biochem. J. 304:687-692(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASN-324. Tissue: Hippocampus.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X79440 mRNA. Translation: CAA55956.1. AP001148 Genomic DNA. No translation available. AP001831 Genomic DNA. No translation available. AP002492 Genomic DNA. No translation available.
IPI	IPI00003970.
PIR	S53351.
RefSeq	NP_001014811.1. NM_001014811.1. NP_001155058.1. NM_001161586.1. NP_006671.2. NM_006680.2.
UniGene	Hs.199743.
3D structure databases
ProteinModelPortal	Q16798.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000352657.
PTM databases
PhosphoSite	Q16798.
Polymorphism databases
DMDM	215274021.
Proteomic databases
PaxDb	Q16798.
PRIDE	Q16798.
Protocols and materials databases
DNASU	10873.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000359636; ENSP00000352657; ENSG00000151376. ENST00000393324; ENSP00000376998; ENSG00000151376. ENST00000543262; ENSP00000440246; ENSG00000151376.
GeneID	10873.
KEGG	hsa:10873.
UCSC	uc001pbz.3. human.
Organism-specific databases
CTD	10873.
GeneCards	GC11M086152.
H-InvDB	HIX0010004.
HGNC	HGNC:6985. ME3.
HPA	HPA038473.
MIM	604626. gene.
neXtProt	NX_Q16798.
PharmGKB	PA30725.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0281.
HOGENOM	HOG000042486.
HOVERGEN	HBG000746.
InParanoid	Q16798.
KO	K00029.
OMA	LASWYPE.
OrthoDB	EOG44BB1T.
PhylomeDB	Q16798.
Enzyme and pathway databases
SABIO-RK	Q16798.
Gene expression databases
ArrayExpress	Q16798.
Bgee	Q16798.
CleanEx	HS_ME3.
Genevestigator	Q16798.
GermOnline	ENSG00000151376. Homo sapiens.
Family and domain databases
Gene3D	3.40.50.10380. 1 hit. 3.40.50.720. 1 hit.
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD-bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PIRSF	PIRSF000106. ME. 1 hit.
PRINTS	PR00072. MALOXRDTASE.
SMART	SM00919. Malic_M. 1 hit. [Graphical view]
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL6182.
ChiTaRS	ME3. human.
DrugBank	DB00157. NADH.
GenomeRNAi	10873.
NextBio	41285.
SOURCE	Search...

Entry information

Entry name

MAON_HUMAN

Accession

Primary (citable) accession number: Q16798

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 120 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents