Reviewed, UniProtKB/Swiss-Prot Q16798 (MAON_HUMAN)

Names and origin

Protein names

Recommended name:
    NADP-dependent malic enzyme, mitochondrial
      Short name=NADP-ME
    EC=1.1.1.40
Alternative name(s):
    Malic enzyme 3

Gene names

Name:

ME3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	604 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subcellular location	Mitochondrion matrix.
Tissue specificity	Expressed predominantly in organs with a low-division rate.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Polymorphism
Domain	Transit peptide
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	aerobic respiration Ref.1 Traceable author statement. Source: UniProtKB malate metabolic process Ref.1 Inferred from direct assay. Source: UniProtKB oxidation reduction Ref.1 Inferred from direct assay. Source: UniProtKB oxygen and reactive oxygen species metabolic process Ref.1 Traceable author statement. Source: UniProtKB pyruvate metabolic process Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity Ref.1 Inferred from direct assay. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 604		NADP-dependent malic enzyme, mitochondrial		PRO_0000018539
Sites
	Active site	137	1	Proton donor By similarity
	Active site	208	1	Proton acceptor By similarity
	Metal binding	280	1	Divalent metal cation By similarity
	Metal binding	281	1	Divalent metal cation By similarity
	Metal binding	304	1	Divalent metal cation By similarity
	Binding site	190	1	NAD By similarity
	Binding site	304	1	NAD By similarity
	Binding site	443	1	NAD By similarity
	Site	304	1	Important for activity By similarity
Natural variations
	Natural variant	85	1	S → G: dbSNP rs17856661.		VAR_047369
	Natural variant	324	1	K → N: dbSNP rs1042780. Ref.1		VAR_047370

Sequences

	Sequence		Length	Mass (Da)
	Q16798-1 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: E0E79A75F3CAC524	FASTA	604	67,068
	10 20 30 40 50 60 MGAALGTGTR LAPWPGRACG ALPRWTPTAP AQGCHSKPGP ARPVPLKKRG YDVTRNPHLN 70 80 90 100 110 120 KGMAFTLEER LQLGIHGLIP PCFLSQDVQL LRIMRYYERQ QSDLDKYIIL MTLQDRNEKL 130 140 150 160 170 180 FYRVLTSDVE KFMPIVYTPT VGLACQHYGL TFRRPRGLFI TIHDKGHLAT MLNSWPEDNI 190 200 210 220 230 240 KAVVVTDGER ILGLGDLGCY GMGIPVGKLA LYTACGGVNP QQCLPVLLDV GTNNEELLRD 250 260 270 280 290 300 PLYIGLKHQR VHGKAYDDLL DEFMQAVTDK FGINCLIQFE DFANANAFRL LNKYRNKYCM 310 320 330 340 350 360 FNDDIQGTAS VAVAGILAAL RITKNKLSNH VFVFQGAGEA AMGIAHLLVM ALEKEGVPKA 370 380 390 400 410 420 EATRKIWMVD SKGLIVKGRS HLNHEKEMFA QDHPEVNSLE EVVRLVKPTA IIGVAAIAGA 430 440 450 460 470 480 FTEQILRDMA SFHERPIIFA LSNPTSKAEC TAEKCYRVTE GRGIFASGSP FKSVTLEDGK 490 500 510 520 530 540 TFIPGQGNNA YVFPGVALGV IAGGIRHIPD EIFLLTAEQI AQEVSEQHLS QGRLYPPLST 550 560 570 580 590 600 IRDVSLRIAI KVLDYAYKHN LASYYPEPKD KEAFVRSLVY TPDYDSFTLD SYTWPKEAMN VQTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification, cDNA cloning and heterologous expression of the human mitochondrial NADP(+)-dependent malic enzyme." Loeber G., Maurer-Fogy I., Schwendenwein R. Biochem. J. 304:687-692(1994) [PubMed: 7818469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASN-324. Tissue: Hippocampus.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X79440 mRNA. Translation: CAA55956.1. AP001148 Genomic DNA. No translation available. AP001831 Genomic DNA. No translation available. AP002492 Genomic DNA. No translation available.
IPI	IPI00003970.
PIR	S53351.
RefSeq	NP_001014811.1. NP_006671.2.
UniGene	Hs.199743
3D structure databases
HSSP	HSSP built from PDB template 1GQ2 based on UniProtKB P40927.
ModBase	Search...
Proteomic databases
PRIDE	Q16798.
Genome annotation databases
Ensembl	ENSG00000151376. Homo sapiens. [Contig view]
GeneID	10873.
KEGG	hsa:10873.
Organism-specific databases
GeneCards	GC11M085829.
H-InvDB	HIX0010004.
HGNC	HGNC:6985. ME3.
MIM	604626. gene.
PharmGKB	PA30725.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q16798.
HOVERGEN	Q16798.
OMA	Q16798. HEKEMFA.
Enzyme and pathway databases
BRENDA	1.1.1.40. 247.
Gene expression databases
ArrayExpress	Q16798.
Bgee	Q16798.
CleanEx	HS_ME3.
GermOnline	ENSG00000151376. Homo sapiens.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00157. NADH.
NextBio	41285.
SOURCE	Search...

Entry information

Entry name

MAON_HUMAN

Accession

Primary (citable) accession number: Q16798

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 25, 2008
Last modified:	June 16, 2009
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)