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Q16798 (MAON_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADP-dependent malic enzyme, mitochondrial

Short name=NADP-ME
EC=1.1.1.40
Alternative name(s):
Malic enzyme 3
Gene names
Name:ME3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length604 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.

Oxaloacetate = pyruvate + CO2.

Cofactor

Divalent metal cations. Prefers magnesium or manganese By similarity.

Subcellular location

Mitochondrion matrix.

Tissue specificity

Expressed predominantly in organs with a low-division rate.

Sequence similarities

Belongs to the malic enzymes family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 604NADP-dependent malic enzyme, mitochondrialPRO_0000018539

Sites

Active site1371Proton donor By similarity
Active site2081Proton acceptor By similarity
Metal binding2801Divalent metal cation By similarity
Metal binding2811Divalent metal cation By similarity
Metal binding3041Divalent metal cation By similarity
Binding site1901NAD By similarity
Binding site3041NAD By similarity
Binding site4431NAD By similarity
Site3041Important for activity By similarity

Natural variations

Natural variant851S → G. Ref.3
Corresponds to variant rs17856661 [ dbSNP | Ensembl ].
VAR_047369
Natural variant3241K → N. Ref.1 Ref.3
Corresponds to variant rs1042780 [ dbSNP | Ensembl ].
VAR_047370

Experimental info

Sequence conflict951R → G in AAH22472. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q16798 [UniParc].

Last modified November 25, 2008. Version 2.
Checksum: E0E79A75F3CAC524

FASTA60467,068
        10         20         30         40         50         60 
MGAALGTGTR LAPWPGRACG ALPRWTPTAP AQGCHSKPGP ARPVPLKKRG YDVTRNPHLN 

        70         80         90        100        110        120 
KGMAFTLEER LQLGIHGLIP PCFLSQDVQL LRIMRYYERQ QSDLDKYIIL MTLQDRNEKL 

       130        140        150        160        170        180 
FYRVLTSDVE KFMPIVYTPT VGLACQHYGL TFRRPRGLFI TIHDKGHLAT MLNSWPEDNI 

       190        200        210        220        230        240 
KAVVVTDGER ILGLGDLGCY GMGIPVGKLA LYTACGGVNP QQCLPVLLDV GTNNEELLRD 

       250        260        270        280        290        300 
PLYIGLKHQR VHGKAYDDLL DEFMQAVTDK FGINCLIQFE DFANANAFRL LNKYRNKYCM 

       310        320        330        340        350        360 
FNDDIQGTAS VAVAGILAAL RITKNKLSNH VFVFQGAGEA AMGIAHLLVM ALEKEGVPKA 

       370        380        390        400        410        420 
EATRKIWMVD SKGLIVKGRS HLNHEKEMFA QDHPEVNSLE EVVRLVKPTA IIGVAAIAGA 

       430        440        450        460        470        480 
FTEQILRDMA SFHERPIIFA LSNPTSKAEC TAEKCYRVTE GRGIFASGSP FKSVTLEDGK 

       490        500        510        520        530        540 
TFIPGQGNNA YVFPGVALGV IAGGIRHIPD EIFLLTAEQI AQEVSEQHLS QGRLYPPLST 

       550        560        570        580        590        600 
IRDVSLRIAI KVLDYAYKHN LASYYPEPKD KEAFVRSLVY TPDYDSFTLD SYTWPKEAMN 


VQTV 

« Hide

References

« Hide 'large scale' references
[1]"Purification, cDNA cloning and heterologous expression of the human mitochondrial NADP(+)-dependent malic enzyme."
Loeber G., Maurer-Fogy I., Schwendenwein R.
Biochem. J. 304:687-692(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASN-324.
Tissue: Hippocampus.
[2]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS GLY-85 AND ASN-324.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79440 mRNA. Translation: CAA55956.1.
AP001148 Genomic DNA. No translation available.
AP001831 Genomic DNA. No translation available.
AP002492 Genomic DNA. No translation available.
BC022472 mRNA. Translation: AAH22472.1.
CCDSCCDS8277.1.
PIRS53351.
RefSeqNP_001014811.1. NM_001014811.1.
NP_001155058.1. NM_001161586.1.
NP_006671.2. NM_006680.2.
XP_005273774.1. XM_005273717.1.
UniGeneHs.199743.

3D structure databases

ProteinModelPortalQ16798.
SMRQ16798. Positions 48-602.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000352657.

Chemistry

ChEMBLCHEMBL6182.
DrugBankDB00157. NADH.

PTM databases

PhosphoSiteQ16798.

Polymorphism databases

DMDM215274021.

Proteomic databases

MaxQBQ16798.
PaxDbQ16798.
PRIDEQ16798.

Protocols and materials databases

DNASU10873.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000359636; ENSP00000352657; ENSG00000151376.
ENST00000393324; ENSP00000376998; ENSG00000151376.
ENST00000543262; ENSP00000440246; ENSG00000151376.
GeneID10873.
KEGGhsa:10873.
UCSCuc001pbz.3. human.

Organism-specific databases

CTD10873.
GeneCardsGC11M086152.
H-InvDBHIX0010004.
HGNCHGNC:6985. ME3.
HPAHPA038473.
MIM604626. gene.
neXtProtNX_Q16798.
PharmGKBPA30725.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0281.
HOGENOMHOG000042486.
HOVERGENHBG000746.
InParanoidQ16798.
KOK00029.
OMADFAYKHN.
OrthoDBEOG7G4QF0.
PhylomeDBQ16798.
TreeFamTF300537.

Enzyme and pathway databases

SABIO-RKQ16798.

Gene expression databases

ArrayExpressQ16798.
BgeeQ16798.
CleanExHS_ME3.
GenevestigatorQ16798.

Family and domain databases

Gene3D3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFPIRSF000106. ME. 1 hit.
PRINTSPR00072. MALOXRDTASE.
SMARTSM00919. Malic_M. 1 hit.
[Graphical view]
PROSITEPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSME3. human.
GenomeRNAi10873.
NextBio41285.
PROQ16798.
SOURCESearch...

Entry information

Entry nameMAON_HUMAN
AccessionPrimary (citable) accession number: Q16798
Secondary accession number(s): Q8TBJ0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 25, 2008
Last modified: July 9, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM