Reviewed,
UniProtKB/Swiss-Prot Q16790 (CAH9_HUMAN)
Last modified
November 25, 2008.
Version 85.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Carbonic anhydrase 9 EC=4.2.1.1 Alternative name(s): Carbonic anhydrase IX Short name=CA-IX Short name=CAIX Carbonate dehydratase IX Membrane antigen MN P54/58N Renal cell carcinoma-associated antigen G250 Short name=RCC-associated antigen G250 pMW1 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 459 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Reversible hydration of carbon dioxide. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. |
| Catalytic activity | H(2)CO(3) = CO(2) + H(2)O. |
| Cofactor | Zinc By similarity. |
| Subunit structure | Forms oligomers linked by disulfide bonds. |
| Subcellular location | Nucleus. Nucleus › nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection › microvillus membrane; Single-pass type I membrane protein. Note= Found on the surface microvilli and in the nucleus, particularly in nucleolus. |
| Tissue specificity | Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. |
| Post-translational modification | N-glycosylated. |
| Sequence similarities | Belongs to the alpha-carbonic anhydrase family. |
Ontologies
Keywords | |
|---|---|
| Cellular component | Cell membrane Cell projection Membrane Nucleus |
| Coding sequence diversity | Polymorphism |
| Domain | Signal Transmembrane |
| Ligand | Metal-binding Zinc |
| Molecular function | Lyase |
| PTM | Glycoprotein |
| Technical term | 3D-structure Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | one-carbon compound metabolic process Inferred from electronic annotation. Source: InterPro |
| Cellular component | cell projection Inferred from electronic annotation. Source: UniProtKB-KW integral to membraneTraceable author statement. Source: ProtInc nucleusInferred from electronic annotation. Source: UniProtKB-KW plasma membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | carbonate dehydratase activity Traceable author statement. Source: ProtInc zinc ion bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 37 | 37 | |||||||
| Chain | 38 – 459 | 422 | Carbonic anhydrase 9 | PRO_0000004243 | |||||
Regions | |||||||||
| Topological domain | 38 – 414 | 377 | Extracellular | ||||||
| Transmembrane | 415 – 435 | 21 | Potential | ||||||
| Topological domain | 436 – 459 | 24 | Cytoplasmic | ||||||
Sites | |||||||||
| Metal binding | 226 | 1 | Zinc; catalytic By similarity | ||||||
| Metal binding | 228 | 1 | Zinc; catalytic By similarity | ||||||
| Metal binding | 251 | 1 | Zinc; catalytic By similarity | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 346 | 1 | N-linked (GlcNAc...) | ||||||
Natural variations | |||||||||
| Natural variant | 33 | 1 | V → M: dbSNP rs2071676. | VAR_010787 | |||||
| Natural variant | 326 | 1 | Q → R: dbSNP rs3829078. | VAR_020049 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and characterization of MN, a human tumor-associated protein with a domain homologous to carbonic anhydrase and a putative helix-loop-helix DNA binding segment." Pastorek J., Pastorekova S., Callebaut I., Mornon J.-P., Zelnik V., Opavsky R., Zat'Ovicova M., Liao S., Portetelle D., Stanbridge E.J., Zavada J., Burny A., Kettmann R. Oncogene 9:2877-2888(1994) [PubMed: 8084592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, VARIANT MET-33. Tissue: Carcinoma. |
| [2] | "Molecular cloning and immunogenicity of renal cell carcinoma-associated antigen G250." Grabmaier K., Vissers J.L.M., De Weijert M.C.A., Oosterwijk-Wakka J.C., Van Bokhoven A., Brakenhoff R.H., Noessner E., Mulders P.A., Merkx G., Figdor C.G., Adema G.J., Oosterwijk E. Int. J. Cancer 85:865-870(2000) [PubMed: 10709109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Renal cell carcinoma. |
| [3] | "DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. Dunham I.Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-33. Tissue: Colon. |
| [6] | "Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract] Cited for: PROTEIN SEQUENCE OF 38-52. |
| [7] | "A novel quasi-viral agent, MaTu, is a two-component system." Pastorekova S., Zavadova Z., Kostal M., Babusikova O., Zavada J. Virology 187:620-626(1992) [PubMed: 1312272] [Abstract] Cited for: CHARACTERIZATION. |
| [8] | "Expression of MaTu-MN protein in human tumor cultures and in clinical specimens." Zavada J., Zavadova Z., Pastorekova S., Ciampor F., Pastorek J., Zelnik V. Int. J. Cancer 54:268-274(1993) [PubMed: 8486430] [Abstract] Cited for: SUBCELLULAR LOCATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X66839 mRNA. Translation: CAA47315.1. AJ010588 mRNA. Translation: CAB82444.1. AL133410, AL357874 Genomic DNA. Translation: CAI10985.1. AL357874, AL133410 Genomic DNA. Translation: CAI13455.1. CH471071 Genomic DNA. Translation: EAW58359.1. BC014950 mRNA. Translation: AAH14950.1. | |||||||||||||
| PIR | I38013. | ||||||||||||
| RefSeq | NP_001207.2. | ||||||||||||
| UniGene | Hs.63287 | ||||||||||||
3D structure databases | |||||||||||||
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| ModBase | Search... | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | Q16790. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSG00000107159. Homo sapiens. [Contig view] | ||||||||||||
| GeneID | 768. | ||||||||||||
| KEGG | hsa:768. | ||||||||||||
Organism-specific databases | |||||||||||||
| HGNC | HGNC:1383. CA9. | ||||||||||||
| HPA | CAB005100. CAB017107. | ||||||||||||
| MIM | 603179. gene. | ||||||||||||
| PharmGKB | PA24384. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
| GeneCards | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | Q16790. | ||||||||||||
| HOVERGEN | Q16790. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | Q16790. | ||||||||||||
| CleanEx | HS_CA9. | ||||||||||||
| GermOnline | ENSG00000107159. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR001148. Euk_COanhd. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:3.10.200.10. Euk_COanhd. 1 hit. | ||||||||||||
| PANTHER | PTHR18952. Euk_COanhd. 1 hit. | ||||||||||||
| Pfam | PF00194. Carb_anhydrase. 1 hit. [Graphical view] | ||||||||||||
| ProDom | PD000865. Euk_COanhd. 1 hit. [Graphical view] [Entries sharing at least one domain] | ||||||||||||
| PROSITE | PS00162. ALPHA_CA_1. 1 hit. PS51144. ALPHA_CA_2. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| NextBio | 3106. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | CAH9_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q16790 Secondary accession number(s): Q5T4R1 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 9 Human chromosome 9: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


