Q16774 (KGUA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanylate kinase
EC=2.7.4.8

Alternative name(s):
GMP kinase

Gene names

Name:	GUK1
Synonyms:	GMK

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for recycling GMP and indirectly, cGMP.
Catalytic activity	ATP + GMP = ADP + GDP.
Subunit structure	Monomer By similarity.
Miscellaneous	In addition to phosphorylating GMP, antiviral prodrugs such as acyclovir, ganciclovir, and carbovir and anticancer prodrugs such as the thiopurines are dependent on GMPK for their activation.
Sequence similarities	Belongs to the guanylate kinase family. Contains 1 guanylate kinase-like domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	drug metabolic process Traceable author statement. Source: Reactome nucleobase-containing small molecule interconversion Traceable author statement. Source: Reactome purine nucleotide metabolic process Inferred from direct assay Ref.2. Source: UniProtKB
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW guanylate kinase activity Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q16774-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q16774-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MLRRPLAGLAAAALGRAPPDGM

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 197	196	Guanylate kinase		PRO_0000170651
Regions
	Domain	4 – 186	183	Guanylate kinase-like
	Nucleotide binding	11 – 18	8	ATP By similarity
Sites
	Active site	44	1	By similarity
	Active site	137	1	By similarity
	Active site	148	1	By similarity
	Binding site	171	1	ATP By similarity
	Binding site	172	1	ATP; via carbonyl oxygen By similarity
Amino acid modifications
	Modified residue	53	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	1	1	M → MLRRPLAGLAAAALGRAPPD GM in isoform 2.		VSP_043778

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: C49573212A0DA825	FASTA	197	21,726
	10 20 30 40 50 60 MSGPRPVVLS GPSGAGKSTL LKRLLQEHSG IFGFSVSHTT RNPRPGEENG KDYYFVTREV 70 80 90 100 110 120 MQRDIAAGDF IEHAEFSGNL YGTSKVAVQA VQAMNRICVL DVDLQGVRNI KATDLRPIYI 130 140 150 160 170 180 SVQPPSLHVL EQRLRQRNTE TEESLVKRLA AAQADMESSK EPGLFDVVII NDSLDQAYAE 190 LKEALSEEIK KAQRTGA
	Isoform 2 [UniParc]. Checksum: 6972858284006BB2	FASTA	218	23,782
	10 20 30 40 50 60 MLRRPLAGLA AAALGRAPPD GMSGPRPVVL SGPSGAGKST LLKRLLQEHS GIFGFSVSHT 70 80 90 100 110 120 TRNPRPGEEN GKDYYFVTRE VMQRDIAAGD FIEHAEFSGN LYGTSKVAVQ AVQAMNRICV 130 140 150 160 170 180 LDVDLQGVRN IKATDLRPIY ISVQPPSLHV LEQRLRQRNT ETEESLVKRL AAAQADMESS 190 200 210 KEPGLFDVVI INDSLDQAYA ELKEALSEEI KKAQRTGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human guanylate kinase (GUK1): cDNA sequence, expression and chromosomal localisation." Fitzgibbon J., Katsanis N., Wells D., Delhanty J., Vallins W., Hunt D.M. FEBS Lett. 385:185-188(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Retina.
[2]	"Cloning, characterization, and modeling of mouse and human guanylate kinases." Brady W.A., Kokoris M.S., Fitzgibbon M., Black M.E. J. Biol. Chem. 271:16734-16740(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Ovary.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L76200 mRNA. Translation: AAC37598.1. U66895 mRNA. Translation: AAC50659.1. AK303845 mRNA. Translation: BAG64788.1. AL359510 Genomic DNA. No translation available. CH471098 Genomic DNA. Translation: EAW69856.1. BC006249 mRNA. Translation: AAH06249.1. BC009914 mRNA. Translation: AAH09914.1.
IPI	IPI00182293.
PIR	S68864.
RefSeq	NP_000849.1. NM_000858.5. NP_001152862.1. NM_001159390.1. NP_001152863.1. NM_001159391.1. NP_001229768.1. NM_001242839.1.
UniGene	Hs.376933.
3D structure databases
ProteinModelPortal	Q16774.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q16774. 2 interactions.
STRING	9606.ENSP00000317659.
PTM databases
PhosphoSite	Q16774.
Polymorphism databases
DMDM	2497498.
Proteomic databases
PaxDb	Q16774.
PRIDE	Q16774.
Protocols and materials databases
DNASU	2987.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000312726; ENSP00000317659; ENSG00000143774. ENST00000366716; ENSP00000355677; ENSG00000143774. ENST00000366718; ENSP00000355679; ENSG00000143774. ENST00000366726; ENSP00000355687; ENSG00000143774. ENST00000366730; ENSP00000355691; ENSG00000143774. ENST00000391865; ENSP00000375738; ENSG00000143774.
GeneID	2987.
KEGG	hsa:2987.
UCSC	uc001hsh.3. human.
Organism-specific databases
CTD	2987.
GeneCards	GC01P228327.
HGNC	HGNC:4693. GUK1.
HPA	HPA048587.
MIM	139270. gene.
neXtProt	NX_Q16774.
PharmGKB	PA29072.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0194.
HOGENOM	HOG000037640.
HOVERGEN	HBG003344.
KO	K00942.
OrthoDB	EOG4NS3CS.
PhylomeDB	Q16774.
Enzyme and pathway databases
BioCyc	MetaCyc:HS07104-MONOMER.
Reactome	REACT_111217. Metabolism.
SABIO-RK	Q16774.
Gene expression databases
ArrayExpress	Q16774.
Bgee	Q16774.
CleanEx	HS_GUK1.
Genevestigator	Q16774.
GermOnline	ENSG00000143774. Homo sapiens.
Family and domain databases
InterPro	IPR008144. Guanylate_kin. IPR008145. Guanylate_kin/L-typ_Ca_channel. IPR020590. Guanylate_kinase_CS. IPR017665. Guanylate_kinase_sub. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF00625. Guanylate_kin. 1 hit. [Graphical view]
SMART	SM00072. GuKc. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR03263. guanyl_kin. 1 hit.
PROSITE	PS00856. GUANYLATE_KINASE_1. 1 hit. PS50052. GUANYLATE_KINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL4989.
ChiTaRS	GUK1. human.
GenomeRNAi	2987.
NextBio	11844.
SOURCE	Search...

Entry information

Entry name

KGUA_HUMAN

Accession

Primary (citable) accession number: Q16774

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.