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Protein

Glutathione S-transferase A3

Gene

GSTA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes isomerization reactions that contribute to the biosynthesis of steroid hormones. Efficiently catalyze obligatory double-bond isomerizations of delta5-androstene-3,17-dione and delta5-pregnene-3,20-dione, precursors to testosterone and progesterone, respectively.2 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Kineticsi

  1. KM=23 µM for delta5-androstene-3,17-dione1 Publication
  1. Vmax=99 µmol/min/mg enzyme for delta5-androstene-3,17-dione isomerization1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9Glutathione2 Publications1
Binding sitei45Glutathione2 Publications1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BRENDAi2.5.1.18 2681
5.3.3.1 2681
ReactomeiR-HSA-156590 Glutathione conjugation
SABIO-RKiQ16772

Chemistry databases

SwissLipidsiSLP:000001612

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase A3 (EC:2.5.1.18)
Alternative name(s):
GST class-alpha member 3
Glutathione S-transferase A3-3
Gene namesi
Name:GSTA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000174156.13
HGNCiHGNC:4628 GSTA3
MIMi605449 gene
neXtProtiNX_Q16772

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi2940
OpenTargetsiENSG00000174156
PharmGKBiPA29018

Chemistry databases

ChEMBLiCHEMBL4866
DrugBankiDB00143 Glutathione
DB04521 GSHNA

Polymorphism and mutation databases

BioMutaiGSTA3
DMDMi21264437

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001857852 – 222Glutathione S-transferase A3Add BLAST221

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei4N6-succinyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ16772
PaxDbiQ16772
PeptideAtlasiQ16772
PRIDEiQ16772
ProteomicsDBi61058

PTM databases

iPTMnetiQ16772
PhosphoSitePlusiQ16772

Expressioni

Gene expression databases

BgeeiENSG00000174156
CleanExiHS_GSTA3
ExpressionAtlasiQ16772 baseline and differential
GenevisibleiQ16772 HS

Organism-specific databases

HPAiHPA004342
HPA048934
HPA053817

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

BioGridi109195, 4 interactors
IntActiQ16772, 1 interactor
STRINGi9606.ENSP00000211122

Structurei

Secondary structure

1222
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 9Combined sources4
Turni14 – 16Combined sources3
Helixi17 – 25Combined sources9
Beta strandi31 – 34Combined sources4
Helixi38 – 46Combined sources9
Beta strandi57 – 60Combined sources4
Beta strandi63 – 67Combined sources5
Helixi68 – 78Combined sources11
Helixi86 – 108Combined sources23
Helixi109 – 111Combined sources3
Helixi114 – 130Combined sources17
Helixi132 – 143Combined sources12
Beta strandi146 – 149Combined sources4
Helixi155 – 170Combined sources16
Turni172 – 177Combined sources6
Helixi179 – 190Combined sources12
Helixi192 – 197Combined sources6
Helixi210 – 220Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TDIX-ray2.40A/B1-222[»]
2VCVX-ray1.80A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-222[»]
ProteinModelPortaliQ16772
SMRiQ16772
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ16772

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 83GST N-terminalAdd BLAST81
Domaini85 – 207GST C-terminalAdd BLAST123

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 55Glutathione binding2 Publications2
Regioni67 – 68Glutathione binding2 Publications2

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated

Phylogenomic databases

eggNOGiKOG1695 Eukaryota
ENOG4111VAU LUCA
GeneTreeiENSGT00670000097856
HOGENOMiHOG000115734
HOVERGENiHBG053749
InParanoidiQ16772
KOiK00799
OMAiKSRYFPA
PhylomeDBiQ16772
TreeFamiTF105321

Family and domain databases

InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR004045 Glutathione_S-Trfase_N
IPR003080 GST_alpha
IPR004046 GST_C
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF14497 GST_C_3, 1 hit
PF02798 GST_N, 1 hit
PRINTSiPR01266 GSTRNSFRASEA
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q16772-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKPKLHYF NGRGRMEPIR WLLAAAGVEF EEKFIGSAED LGKLRNDGSL
60 70 80 90 100
MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYTEGMA
110 120 130 140 150
DLNEMILLLP LCRPEEKDAK IALIKEKTKS RYFPAFEKVL QSHGQDYLVG
160 170 180 190 200
NKLSRADISL VELLYYVEEL DSSLISNFPL LKALKTRISN LPTVKKFLQP
210 220
GSPRKPPADA KALEEARKIF RF
Length:222
Mass (Da):25,302
Last modified:May 27, 2002 - v3
Checksum:i904AA17519B5343C
GO

Sequence cautioni

The sequence AAA74634 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAD04712 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti63M → I in AAH20619 (PubMed:15489334).Curated1
Sequence conflicti113 – 114RP → PA in AAA74634 (PubMed:8307579).Curated2
Sequence conflicti128T → I in AAA74634 (PubMed:8307579).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06227636G → E1 PublicationCorresponds to variant dbSNP:rs45504096Ensembl.1
Natural variantiVAR_04948471I → L2 PublicationsCorresponds to variant dbSNP:rs1052661Ensembl.1
Natural variantiVAR_04948573N → D1 PublicationCorresponds to variant dbSNP:rs41273858Ensembl.1
Natural variantiVAR_062277113R → Q1 PublicationCorresponds to variant dbSNP:rs45602042Ensembl.1
Natural variantiVAR_062278208A → T1 PublicationCorresponds to variant dbSNP:rs45620832Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13275
, L13270, L13271, L13272, L13273, L13274 Genomic DNA Translation: AAA74634.1 Different initiation.
AF508266 mRNA Translation: AAM33360.1
DQ993361 Genomic DNA Translation: ABI75350.1
AL121969 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX04391.1
BC020619 mRNA Translation: AAH20619.1
AF020919 mRNA Translation: AAD04712.1 Different initiation.
CCDSiCCDS4947.1
PIRiA49365
RefSeqiNP_000838.3, NM_000847.4
UniGeneiHs.102484

Genome annotation databases

EnsembliENST00000211122; ENSP00000211122; ENSG00000174156
GeneIDi2940
KEGGihsa:2940
UCSCiuc003pbb.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiGSTA3_HUMAN
AccessioniPrimary (citable) accession number: Q16772
Secondary accession number(s): O43468
, Q068V6, Q8WWA8, Q9H415
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 27, 2002
Last modified: June 20, 2018
This is version 165 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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