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Protein

Glutathione S-transferase A3

Gene

GSTA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes isomerization reactions that contribute to the biosynthesis of steroid hormones. Efficiently catalyze obligatory double-bond isomerizations of delta(5)-androstene-3,17-dione and delta(5)-pregnene-3,20-dione, precursors to testosterone and progesterone, respectively.2 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Kineticsi

  1. KM=23 µM for delta(5)-androstene-3,17-dione1 Publication
  1. Vmax=99 µmol/min/mg enzyme for delta(5)-androstene-3,17-dione isomerization1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9Glutathione2 Publications1
Binding sitei45Glutathione2 Publications1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciZFISH:HS07329-MONOMER.
ZFISH:HS10772-MONOMER.
BRENDAi2.5.1.18. 2681.
5.3.3.1. 2681.
ReactomeiR-HSA-156590. Glutathione conjugation.
SABIO-RKQ16772.

Chemistry databases

SwissLipidsiSLP:000001612.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase A3 (EC:2.5.1.18)
Alternative name(s):
GST class-alpha member 3
Glutathione S-transferase A3-3
Gene namesi
Name:GSTA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:4628. GSTA3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi2940.
OpenTargetsiENSG00000174156.
PharmGKBiPA29018.

Chemistry databases

ChEMBLiCHEMBL4866.
DrugBankiDB00143. Glutathione.

Polymorphism and mutation databases

BioMutaiGSTA3.
DMDMi21264437.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001857852 – 222Glutathione S-transferase A3Add BLAST221

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei4N6-succinyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ16772.
PaxDbiQ16772.
PeptideAtlasiQ16772.
PRIDEiQ16772.

PTM databases

iPTMnetiQ16772.
PhosphoSitePlusiQ16772.

Expressioni

Gene expression databases

BgeeiENSG00000174156.
CleanExiHS_GSTA3.
ExpressionAtlasiQ16772. baseline and differential.
GenevisibleiQ16772. HS.

Organism-specific databases

HPAiHPA004342.
HPA048934.
HPA053817.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

BioGridi109195. 4 interactors.
IntActiQ16772. 1 interactor.
STRINGi9606.ENSP00000211122.

Structurei

Secondary structure

1222
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 9Combined sources4
Turni14 – 16Combined sources3
Helixi17 – 25Combined sources9
Beta strandi31 – 34Combined sources4
Helixi38 – 46Combined sources9
Beta strandi57 – 60Combined sources4
Beta strandi63 – 67Combined sources5
Helixi68 – 78Combined sources11
Helixi86 – 108Combined sources23
Helixi109 – 111Combined sources3
Helixi114 – 130Combined sources17
Helixi132 – 143Combined sources12
Beta strandi146 – 149Combined sources4
Helixi155 – 170Combined sources16
Turni172 – 177Combined sources6
Helixi179 – 190Combined sources12
Helixi192 – 197Combined sources6
Helixi210 – 220Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TDIX-ray2.40A/B1-222[»]
2VCVX-ray1.80A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-222[»]
ProteinModelPortaliQ16772.
SMRiQ16772.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ16772.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 83GST N-terminalAdd BLAST81
Domaini85 – 207GST C-terminalAdd BLAST123

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 55Glutathione binding2 Publications2
Regioni67 – 68Glutathione binding2 Publications2

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiQ16772.
KOiK00799.
OMAiCPPDQKE.
PhylomeDBiQ16772.
TreeFamiTF105321.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q16772-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKPKLHYF NGRGRMEPIR WLLAAAGVEF EEKFIGSAED LGKLRNDGSL
60 70 80 90 100
MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYTEGMA
110 120 130 140 150
DLNEMILLLP LCRPEEKDAK IALIKEKTKS RYFPAFEKVL QSHGQDYLVG
160 170 180 190 200
NKLSRADISL VELLYYVEEL DSSLISNFPL LKALKTRISN LPTVKKFLQP
210 220
GSPRKPPADA KALEEARKIF RF
Length:222
Mass (Da):25,302
Last modified:May 27, 2002 - v3
Checksum:i904AA17519B5343C
GO

Sequence cautioni

The sequence AAA74634 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAD04712 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti63M → I in AAH20619 (PubMed:15489334).Curated1
Sequence conflicti113 – 114RP → PA in AAA74634 (PubMed:8307579).Curated2
Sequence conflicti128T → I in AAA74634 (PubMed:8307579).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06227636G → E.1 PublicationCorresponds to variant rs45504096dbSNPEnsembl.1
Natural variantiVAR_04948471I → L.2 PublicationsCorresponds to variant rs1052661dbSNPEnsembl.1
Natural variantiVAR_04948573N → D.1 PublicationCorresponds to variant rs41273858dbSNPEnsembl.1
Natural variantiVAR_062277113R → Q.1 PublicationCorresponds to variant rs45602042dbSNPEnsembl.1
Natural variantiVAR_062278208A → T.1 PublicationCorresponds to variant rs45620832dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13275
, L13270, L13271, L13272, L13273, L13274 Genomic DNA. Translation: AAA74634.1. Different initiation.
AF508266 mRNA. Translation: AAM33360.1.
DQ993361 Genomic DNA. Translation: ABI75350.1.
AL121969 Genomic DNA. Translation: CAC10389.1.
CH471081 Genomic DNA. Translation: EAX04391.1.
BC020619 mRNA. Translation: AAH20619.1.
AF020919 mRNA. Translation: AAD04712.1. Different initiation.
CCDSiCCDS4947.1.
PIRiA49365.
RefSeqiNP_000838.3. NM_000847.4.
UniGeneiHs.102484.

Genome annotation databases

EnsembliENST00000211122; ENSP00000211122; ENSG00000174156.
GeneIDi2940.
KEGGihsa:2940.
UCSCiuc003pbb.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13275
, L13270, L13271, L13272, L13273, L13274 Genomic DNA. Translation: AAA74634.1. Different initiation.
AF508266 mRNA. Translation: AAM33360.1.
DQ993361 Genomic DNA. Translation: ABI75350.1.
AL121969 Genomic DNA. Translation: CAC10389.1.
CH471081 Genomic DNA. Translation: EAX04391.1.
BC020619 mRNA. Translation: AAH20619.1.
AF020919 mRNA. Translation: AAD04712.1. Different initiation.
CCDSiCCDS4947.1.
PIRiA49365.
RefSeqiNP_000838.3. NM_000847.4.
UniGeneiHs.102484.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TDIX-ray2.40A/B1-222[»]
2VCVX-ray1.80A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-222[»]
ProteinModelPortaliQ16772.
SMRiQ16772.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109195. 4 interactors.
IntActiQ16772. 1 interactor.
STRINGi9606.ENSP00000211122.

Chemistry databases

ChEMBLiCHEMBL4866.
DrugBankiDB00143. Glutathione.
SwissLipidsiSLP:000001612.

PTM databases

iPTMnetiQ16772.
PhosphoSitePlusiQ16772.

Polymorphism and mutation databases

BioMutaiGSTA3.
DMDMi21264437.

Proteomic databases

MaxQBiQ16772.
PaxDbiQ16772.
PeptideAtlasiQ16772.
PRIDEiQ16772.

Protocols and materials databases

DNASUi2940.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000211122; ENSP00000211122; ENSG00000174156.
GeneIDi2940.
KEGGihsa:2940.
UCSCiuc003pbb.4. human.

Organism-specific databases

CTDi2940.
DisGeNETi2940.
GeneCardsiGSTA3.
HGNCiHGNC:4628. GSTA3.
HPAiHPA004342.
HPA048934.
HPA053817.
MIMi605449. gene.
neXtProtiNX_Q16772.
OpenTargetsiENSG00000174156.
PharmGKBiPA29018.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiQ16772.
KOiK00799.
OMAiCPPDQKE.
PhylomeDBiQ16772.
TreeFamiTF105321.

Enzyme and pathway databases

BioCyciZFISH:HS07329-MONOMER.
ZFISH:HS10772-MONOMER.
BRENDAi2.5.1.18. 2681.
5.3.3.1. 2681.
ReactomeiR-HSA-156590. Glutathione conjugation.
SABIO-RKQ16772.

Miscellaneous databases

EvolutionaryTraceiQ16772.
GeneWikiiGSTA3.
GenomeRNAii2940.
PROiQ16772.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000174156.
CleanExiHS_GSTA3.
ExpressionAtlasiQ16772. baseline and differential.
GenevisibleiQ16772. HS.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTA3_HUMAN
AccessioniPrimary (citable) accession number: Q16772
Secondary accession number(s): O43468
, Q068V6, Q8WWA8, Q9H415
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 27, 2002
Last modified: November 30, 2016
This is version 157 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.