Reviewed, UniProtKB/Swiss-Prot Q16763 (UBE2S_HUMAN)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 S
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase S
    Ubiquitin carrier protein S
    Ubiquitin-conjugating enzyme E2-24 kDa
    E2-EPF5

Gene names

Name:	UBE2S
Synonyms:	E2EPF
ORF Names:	OK/SW-cl.73

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Ligase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 222

222

Ubiquitin-conjugating enzyme E2 S

PRO_0000082507

Sites

Active site

95

1

Glycyl thioester intermediate By similarity

Experimental info

Sequence conflict

221 – 222

2

RL → ALRRL Ref.1

Secondary structure

1

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222

Helix Strand Turn

Details...

Helix

10 – 25

16

Beta strand

31 – 35

5

Beta strand

42 – 48

7

Turn

54 – 57

4

Beta strand

59 – 65

7

Turn

68 – 72

5

Beta strand

76 – 81

6

Beta strand

92 – 94

3

Helix

96 – 99

4

Turn

100 – 102

3

Helix

109 – 121

13

Helix

125 – 127

3

Helix

131 – 139

9

Helix

141 – 155

15

Sequences

	Sequence		Length	Mass (Da)
	Q16763-1 [UniParc]. Last modified October 10, 2002. Version 2. Checksum: 2842DC3DCD2AFCB5	FASTA	222	23,845
	10 20 30 40 50 60 MNSNVENLPP HIIRLVYKEV TTLTADPPDG IKVFPNEEDL TDLQVTIEGP EGTPYAGGLF 70 80 90 100 110 120 RMKLLLGKDF PASPPKGYFL TKIFHPNVGA NGEICVNVLK RDWTAELGIR HVLLTIKCLL 130 140 150 160 170 180 IHPNPESALN EEAGRLLLEN YEEYAARARL LTEIHGGAGG PSGRAEAGRA LASGTEASST 190 200 210 220 DPGAPGGPGG AEGPMAKKHA GERDKKLAAK KKTDKKRALR RL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning of a novel human ubiquitin carrier protein. An antigenic domain specifically recognized by endemic pemphigus foliaceus autoantibodies is encoded in a secondary reading frame of this human epidermal transcript." Liu Z., Diaz L.A., Haas A.L., Giudice G.J. J. Biol. Chem. 267:15829-15835(1992) [PubMed: 1379239] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Foreskin.
[2]	"Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients." Shichijo S., Itoh K. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon adenocarcinoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon and Skin.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M91670 mRNA. Translation: AAA58446.1.
AB062397 mRNA. Translation: BAB93484.1.
BC004236 mRNA. Translation: AAH04236.1.
BC007554 mRNA. Translation: AAH07554.1.
BC065364 mRNA. Translation: AAH65364.1.

RefSeq

NP_055316.2.

UniGene

Hs.396393
Hs.592114

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZDN	X-ray	1.93	A/B	1-156	[»]

ModBase

Search...

PTM databases

PhosphoSite

Q16763.

Proteomic databases

PeptideAtlas

Q16763.

Genome annotation databases

Ensembl

ENSG00000108106. Homo sapiens. [Contig view]

GeneID

27338.

KEGG

hsa:27338.

Organism-specific databases

H-InvDB

HIX0015468.
HIX0039201.

HGNC

HGNC:17895. UBE2S.

HPA

CAB015228.

MIM

610309. gene.

PharmGKB

PA134904441.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

Q16763.

HOVERGEN

Q16763.

Gene expression databases

CleanEx

HS_UBE2S.

GermOnline

ENSG00000108106. Homo sapiens.

Family and domain databases

InterPro

IPR016135. UBQ-conjugat/RWD-like.
IPR000608. UBQ-conjugat_E2.
[Graphical view]

Gene3D

G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.

PANTHER

PTHR11621. UBQ-conjugat_E2. 1 hit.

Pfam

PF00179. UQ_con. 1 hit.
[Graphical view]

ProDom

PD000461. UBQ_conjugat. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00212. UBCc. 1 hit.
[Graphical view]

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

Q16763.

NextBio

50400.

SOURCE

Search...

Entry information

Entry name

UBE2S_HUMAN

Accession

Primary (citable) accession number: Q16763
Secondary accession number(s): Q9BTC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 10, 2002
Last modified:	November 25, 2008
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)