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Protein

Ubiquitin-conjugating enzyme E2 S

Gene

UBE2S

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.5 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation3 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation2 Publications
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei95 – 951Glycyl thioester intermediate

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin conjugating enzyme activity Source: MGI
  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin protein ligase binding Source: GO_Central
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • anaphase-promoting complex-dependent catabolic process Source: UniProtKB
  • cell division Source: UniProtKB-KW
  • cellular protein modification process Source: ProtInc
  • exit from mitosis Source: UniProtKB
  • free ubiquitin chain polymerization Source: UniProtKB
  • positive regulation of ubiquitin protein ligase activity Source: UniProtKB
  • protein K11-linked ubiquitination Source: UniProtKB
  • protein K27-linked ubiquitination Source: UniProtKB
  • protein K29-linked ubiquitination Source: UniProtKB
  • protein K63-linked ubiquitination Source: UniProtKB
  • protein K6-linked ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cell cycle, Cell division, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.3.2.B6. 2681.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ16763.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 S (EC:2.3.2.233 Publications)
Alternative name(s):
E2 ubiquitin-conjugating enzyme S
E2-EPF
Ubiquitin carrier protein S
Ubiquitin-conjugating enzyme E2-24 kDa
Ubiquitin-conjugating enzyme E2-EPF5
Ubiquitin-protein ligase S
Gene namesi
Name:UBE2S
Synonyms:E2EPF
ORF Names:OK/SW-cl.73
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:17895. UBE2S.

Subcellular locationi

GO - Cellular componenti

  • anaphase-promoting complex Source: UniProtKB
  • cytoplasm Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi95 – 951C → S: Loss of function. 2 Publications

Organism-specific databases

PharmGKBiPA134904441.

Polymorphism and mutation databases

BioMutaiUBE2S.
DMDMi23829978.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 222222Ubiquitin-conjugating enzyme E2 SPRO_0000082507Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources
Modified residuei173 – 1731PhosphoserineCombined sources

Post-translational modificationi

Autoubiquitinated by the APC/C complex during G1, leading to its degradation by the proteasome.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ16763.
MaxQBiQ16763.
PaxDbiQ16763.
PeptideAtlasiQ16763.
PRIDEiQ16763.

PTM databases

iPTMnetiQ16763.
PhosphoSiteiQ16763.

Expressioni

Gene expression databases

BgeeiENSG00000108106.
CleanExiHS_UBE2S.
ExpressionAtlasiQ16763. baseline and differential.
GenevisibleiQ16763. HS.

Organism-specific databases

HPAiCAB015228.
HPA057150.

Interactioni

Subunit structurei

Component of the APC/C complex. Interacts with CDC20, FZR1/CDH1 and VHL.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi118150. 64 interactions.
DIPiDIP-52784N.
IntActiQ16763. 11 interactions.
MINTiMINT-6774405.
STRINGi9606.ENSP00000264552.

Structurei

Secondary structure

1
222
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 2516Combined sources
Beta strandi31 – 355Combined sources
Beta strandi42 – 487Combined sources
Turni54 – 574Combined sources
Beta strandi59 – 657Combined sources
Turni68 – 725Combined sources
Beta strandi76 – 816Combined sources
Beta strandi92 – 943Combined sources
Helixi96 – 994Combined sources
Turni100 – 1023Combined sources
Helixi109 – 12113Combined sources
Helixi125 – 1273Combined sources
Helixi131 – 1399Combined sources
Helixi141 – 15515Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZDNX-ray1.93A/B1-156[»]
5BNBX-ray2.49A/B/C/D1-156[»]
ProteinModelPortaliQ16763.
SMRiQ16763. Positions 6-156.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ16763.

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0423. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ16763.
KOiK10583.
OrthoDBiEOG091G0USF.
PhylomeDBiQ16763.
TreeFamiTF101120.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q16763-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSNVENLPP HIIRLVYKEV TTLTADPPDG IKVFPNEEDL TDLQVTIEGP
60 70 80 90 100
EGTPYAGGLF RMKLLLGKDF PASPPKGYFL TKIFHPNVGA NGEICVNVLK
110 120 130 140 150
RDWTAELGIR HVLLTIKCLL IHPNPESALN EEAGRLLLEN YEEYAARARL
160 170 180 190 200
LTEIHGGAGG PSGRAEAGRA LASGTEASST DPGAPGGPGG AEGPMAKKHA
210 220
GERDKKLAAK KKTDKKRALR RL
Length:222
Mass (Da):23,845
Last modified:October 10, 2002 - v2
Checksum:i2842DC3DCD2AFCB5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti216 – 2161K → KRAL in AAA58446 (PubMed:1379239).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91670 mRNA. Translation: AAA58446.1.
AB062397 mRNA. Translation: BAB93484.1.
AC020922 Genomic DNA. No translation available.
BC004236 mRNA. Translation: AAH04236.1.
BC007554 mRNA. Translation: AAH07554.1.
BC065364 mRNA. Translation: AAH65364.1.
CCDSiCCDS33114.1.
RefSeqiNP_055316.2. NM_014501.2.
UniGeneiHs.396393.

Genome annotation databases

EnsembliENST00000264552; ENSP00000264552; ENSG00000108106.
GeneIDi27338.
KEGGihsa:27338.
UCSCiuc002qkx.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91670 mRNA. Translation: AAA58446.1.
AB062397 mRNA. Translation: BAB93484.1.
AC020922 Genomic DNA. No translation available.
BC004236 mRNA. Translation: AAH04236.1.
BC007554 mRNA. Translation: AAH07554.1.
BC065364 mRNA. Translation: AAH65364.1.
CCDSiCCDS33114.1.
RefSeqiNP_055316.2. NM_014501.2.
UniGeneiHs.396393.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZDNX-ray1.93A/B1-156[»]
5BNBX-ray2.49A/B/C/D1-156[»]
ProteinModelPortaliQ16763.
SMRiQ16763. Positions 6-156.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118150. 64 interactions.
DIPiDIP-52784N.
IntActiQ16763. 11 interactions.
MINTiMINT-6774405.
STRINGi9606.ENSP00000264552.

PTM databases

iPTMnetiQ16763.
PhosphoSiteiQ16763.

Polymorphism and mutation databases

BioMutaiUBE2S.
DMDMi23829978.

Proteomic databases

EPDiQ16763.
MaxQBiQ16763.
PaxDbiQ16763.
PeptideAtlasiQ16763.
PRIDEiQ16763.

Protocols and materials databases

DNASUi27338.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264552; ENSP00000264552; ENSG00000108106.
GeneIDi27338.
KEGGihsa:27338.
UCSCiuc002qkx.2. human.

Organism-specific databases

CTDi27338.
GeneCardsiUBE2S.
H-InvDBHIX0039201.
HGNCiHGNC:17895. UBE2S.
HPAiCAB015228.
HPA057150.
MIMi610309. gene.
neXtProtiNX_Q16763.
PharmGKBiPA134904441.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0423. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ16763.
KOiK10583.
OrthoDBiEOG091G0USF.
PhylomeDBiQ16763.
TreeFamiTF101120.

Enzyme and pathway databases

UniPathwayiUPA00143.
BRENDAi2.3.2.B6. 2681.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ16763.

Miscellaneous databases

ChiTaRSiUBE2S. human.
EvolutionaryTraceiQ16763.
GenomeRNAii27338.
PROiQ16763.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000108106.
CleanExiHS_UBE2S.
ExpressionAtlasiQ16763. baseline and differential.
GenevisibleiQ16763. HS.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBE2S_HUMAN
AccessioniPrimary (citable) accession number: Q16763
Secondary accession number(s): Q9BTC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 10, 2002
Last modified: September 7, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.