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Protein

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

Gene

NDUFA5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

GO - Molecular functioni

  • NADH dehydrogenase (ubiquinone) activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Enzyme and pathway databases

ReactomeiR-HSA-611105. Respiratory electron transport.
R-HSA-6799198. Complex I biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
Alternative name(s):
Complex I subunit B13
Complex I-13kD-B
Short name:
CI-13kD-B
NADH-ubiquinone oxidoreductase 13 kDa-B subunit
Gene namesi
Name:NDUFA5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:7688. NDUFA5.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: Reactome
  • mitochondrial respiratory chain complex I Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31494.

Chemistry

ChEMBLiCHEMBL2363065.

Polymorphism and mutation databases

DMDMi2499316.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 116115NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5PRO_0000118632Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei30 – 301N6-acetyllysineCombined sources
Modified residuei46 – 461N6-acetyllysineBy similarity
Modified residuei60 – 601N6-acetyllysineCombined sources
Modified residuei98 – 981N6-acetyllysine; alternateBy similarity
Modified residuei98 – 981N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ16718.
MaxQBiQ16718.
PaxDbiQ16718.
PeptideAtlasiQ16718.
PRIDEiQ16718.
TopDownProteomicsiQ16718-1. [Q16718-1]

PTM databases

iPTMnetiQ16718.
PhosphoSiteiQ16718.
SwissPalmiQ16718.

Expressioni

Tissue specificityi

Expressed in all tissues examined with highest levels in heart, skeletal muscle and brain.

Gene expression databases

BgeeiQ16718.
CleanExiHS_NDUFA5.
ExpressionAtlasiQ16718. baseline and differential.
GenevisibleiQ16718. HS.

Organism-specific databases

HPAiHPA043175.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits.1 Publication

Protein-protein interaction databases

BioGridi110778. 29 interactions.
IntActiQ16718. 25 interactions.
STRINGi9606.ENSP00000347988.

Structurei

3D structure databases

ProteinModelPortaliQ16718.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I NDUFA5 subunit family.Curated

Phylogenomic databases

eggNOGiKOG3365. Eukaryota.
ENOG4111V89. LUCA.
GeneTreeiENSGT00390000008099.
HOGENOMiHOG000240009.
HOVERGENiHBG001142.
InParanoidiQ16718.
KOiK03949.
OMAiMPQDAAY.
OrthoDBiEOG73JKXP.
PhylomeDBiQ16718.
TreeFamiTF313785.

Family and domain databases

InterProiIPR006806. ETC_CI_29_9.
[Graphical view]
PfamiPF04716. ETC_C1_NDUFA5. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q16718-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGVLKKTTG LVGLAVCNTP HERLRILYTK ILDVLEEIPK NAAYRKYTEQ
60 70 80 90 100
ITNEKLAMVK AEPDVKKLED QLQGGQLEEV ILQAEHELNL ARKMREWKLW
110
EPLVEEPPAD QWKWPI
Length:116
Mass (Da):13,459
Last modified:January 23, 2007 - v3
Checksum:i652339C1127E6F22
GO
Isoform 2 (identifier: Q16718-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: MAGVLKK → MPYRVGQ

Note: No experimental confirmation available.
Show »
Length:116
Mass (Da):13,563
Checksum:iBD8CF570C1ED77EC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 521T → I in AAH70236 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 77MAGVLKK → MPYRVGQ in isoform 2. 1 PublicationVSP_055164

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53468 mRNA. Translation: AAB02224.1.
U64028 mRNA. Translation: AAB37259.1.
AF044418
, AF044415, AF044416, AF044417 Genomic DNA. Translation: AAD21526.1.
BT006695 mRNA. Translation: AAP35341.1.
AK315458 mRNA. Translation: BAG37845.1.
CR933664 mRNA. Translation: CAI45962.1.
AC073323 Genomic DNA. Translation: AAQ96854.1.
CH471070 Genomic DNA. Translation: EAW83588.1.
BC000813 mRNA. Translation: AAH00813.1.
BC020821 mRNA. Translation: AAH20821.1.
BC070236 mRNA. Translation: AAH70236.1.
BC070237 mRNA. Translation: AAH70237.1.
CCDSiCCDS5788.1. [Q16718-1]
CCDS64760.1. [Q16718-2]
PIRiG02526.
RefSeqiNP_001269348.1. NM_001282419.2.
NP_001269349.1. NM_001282420.2. [Q16718-2]
NP_001269350.1. NM_001282421.2.
NP_001269351.1. NM_001282422.2.
NP_004991.1. NM_005000.4. [Q16718-1]
UniGeneiHs.651219.

Genome annotation databases

EnsembliENST00000355749; ENSP00000347988; ENSG00000128609. [Q16718-1]
ENST00000471770; ENSP00000417142; ENSG00000128609. [Q16718-2]
GeneIDi4698.
KEGGihsa:4698.
UCSCiuc003vks.4. human. [Q16718-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53468 mRNA. Translation: AAB02224.1.
U64028 mRNA. Translation: AAB37259.1.
AF044418
, AF044415, AF044416, AF044417 Genomic DNA. Translation: AAD21526.1.
BT006695 mRNA. Translation: AAP35341.1.
AK315458 mRNA. Translation: BAG37845.1.
CR933664 mRNA. Translation: CAI45962.1.
AC073323 Genomic DNA. Translation: AAQ96854.1.
CH471070 Genomic DNA. Translation: EAW83588.1.
BC000813 mRNA. Translation: AAH00813.1.
BC020821 mRNA. Translation: AAH20821.1.
BC070236 mRNA. Translation: AAH70236.1.
BC070237 mRNA. Translation: AAH70237.1.
CCDSiCCDS5788.1. [Q16718-1]
CCDS64760.1. [Q16718-2]
PIRiG02526.
RefSeqiNP_001269348.1. NM_001282419.2.
NP_001269349.1. NM_001282420.2. [Q16718-2]
NP_001269350.1. NM_001282421.2.
NP_001269351.1. NM_001282422.2.
NP_004991.1. NM_005000.4. [Q16718-1]
UniGeneiHs.651219.

3D structure databases

ProteinModelPortaliQ16718.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110778. 29 interactions.
IntActiQ16718. 25 interactions.
STRINGi9606.ENSP00000347988.

Chemistry

ChEMBLiCHEMBL2363065.

PTM databases

iPTMnetiQ16718.
PhosphoSiteiQ16718.
SwissPalmiQ16718.

Polymorphism and mutation databases

DMDMi2499316.

Proteomic databases

EPDiQ16718.
MaxQBiQ16718.
PaxDbiQ16718.
PeptideAtlasiQ16718.
PRIDEiQ16718.
TopDownProteomicsiQ16718-1. [Q16718-1]

Protocols and materials databases

DNASUi4698.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000355749; ENSP00000347988; ENSG00000128609. [Q16718-1]
ENST00000471770; ENSP00000417142; ENSG00000128609. [Q16718-2]
GeneIDi4698.
KEGGihsa:4698.
UCSCiuc003vks.4. human. [Q16718-1]

Organism-specific databases

CTDi4698.
GeneCardsiNDUFA5.
HGNCiHGNC:7688. NDUFA5.
HPAiHPA043175.
MIMi601677. gene.
neXtProtiNX_Q16718.
PharmGKBiPA31494.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3365. Eukaryota.
ENOG4111V89. LUCA.
GeneTreeiENSGT00390000008099.
HOGENOMiHOG000240009.
HOVERGENiHBG001142.
InParanoidiQ16718.
KOiK03949.
OMAiMPQDAAY.
OrthoDBiEOG73JKXP.
PhylomeDBiQ16718.
TreeFamiTF313785.

Enzyme and pathway databases

ReactomeiR-HSA-611105. Respiratory electron transport.
R-HSA-6799198. Complex I biogenesis.

Miscellaneous databases

ChiTaRSiNDUFA5. human.
GeneWikiiNDUFA5.
GenomeRNAii4698.
PROiQ16718.
SOURCEiSearch...

Gene expression databases

BgeeiQ16718.
CleanExiHS_NDUFA5.
ExpressionAtlasiQ16718. baseline and differential.
GenevisibleiQ16718. HS.

Family and domain databases

InterProiIPR006806. ETC_CI_29_9.
[Graphical view]
PfamiPF04716. ETC_C1_NDUFA5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A human cDNA encoding the homologue of NADH:ubiquinone oxidoreductase subunit B13."
    Pata I., Tensing K., Metspalu A.
    Biochim. Biophys. Acta 1350:115-118(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Cloning of the human NADH:ubiquinone oxidoreductase subunit B13: localization to chromosome 7q32 and identification of a pseudogene on 11p15."
    Russell M.W., du Manoir S., Collins F.S., Brody L.C.
    Mamm. Genome 8:60-61(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "Genomic organization of the human complex I 13-kDa subunit gene NDUFA5."
    Tensing K., Pata I., Wittig I., Wehnert M., Metspalu A.
    Cytogenet. Cell Genet. 84:125-127(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Retina.
  7. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung, Placenta and Skeletal muscle.
  10. "The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification."
    Murray J., Zhang B., Taylor S.W., Oglesbee D., Fahy E., Marusich M.F., Ghosh S.S., Capaldi R.A.
    J. Biol. Chem. 278:13619-13622(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE NADH-UBIQUINONE OXIDOREDUCTASE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  11. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-30 AND LYS-60, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNDUA5_HUMAN
AccessioniPrimary (citable) accession number: Q16718
Secondary accession number(s): B2RD98, Q5H9R2, Q6IRX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.