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Q16706

- MA2A1_HUMAN

UniProt

Q16706 - MA2A1_HUMAN

Protein

Alpha-mannosidase 2

Gene

MAN2A1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (01 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.

    Catalytic activityi

    Hydrolysis of the terminal (1->3)- and (1->6)-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man5(GlcNAc)3.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi175 – 1751ZincBy similarity
    Metal bindingi177 – 1771ZincBy similarity
    Active sitei289 – 2891NucleophileBy similarity
    Metal bindingi289 – 2891ZincBy similarity
    Metal bindingi569 – 5691ZincBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. hydrolase activity, hydrolyzing N-glycosyl compounds Source: Ensembl
    3. mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity Source: ProtInc
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cellular protein metabolic process Source: Reactome
    2. in utero embryonic development Source: Ensembl
    3. liver development Source: Ensembl
    4. lung alveolus development Source: Ensembl
    5. mannose metabolic process Source: InterPro
    6. mitochondrion organization Source: Ensembl
    7. N-glycan processing Source: Ensembl
    8. positive regulation of neurogenesis Source: Ensembl
    9. post-translational protein modification Source: Reactome
    10. protein N-linked glycosylation via asparagine Source: Reactome
    11. respiratory gaseous exchange Source: Ensembl
    12. retina morphogenesis in camera-type eye Source: Ensembl
    13. vacuole organization Source: Ensembl

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:HS03629-MONOMER.
    ReactomeiREACT_25302. Reactions specific to the complex N-glycan synthesis pathway.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGH38. Glycoside Hydrolase Family 38.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-mannosidase 2 (EC:3.2.1.114)
    Alternative name(s):
    Golgi alpha-mannosidase II
    Short name:
    AMan II
    Short name:
    Man II
    Mannosidase alpha class 2A member 1
    Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
    Gene namesi
    Name:MAN2A1
    Synonyms:MANA2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:6824. MAN2A1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular vesicular exosome Source: UniProt
    2. Golgi membrane Source: Reactome
    3. integral component of membrane Source: UniProtKB-KW
    4. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30573.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11441144Alpha-mannosidase 2PRO_0000206902Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi78 – 781N-linked (GlcNAc...)1 Publication
    Modified residuei80 – 801PhosphoserineBy similarity
    Modified residuei82 – 821PhosphoserineBy similarity
    Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1125 – 11251N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ16706.
    PaxDbiQ16706.
    PRIDEiQ16706.

    PTM databases

    PhosphoSiteiQ16706.

    Expressioni

    Gene expression databases

    ArrayExpressiQ16706.
    BgeeiQ16706.
    CleanExiHS_MAN2A1.
    GenevestigatoriQ16706.

    Organism-specific databases

    HPAiCAB022377.
    HPA026896.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.By similarity

    Protein-protein interaction databases

    BioGridi110297. 4 interactions.
    IntActiQ16706. 1 interaction.
    MINTiMINT-2807655.
    STRINGi9606.ENSP00000261483.

    Structurei

    3D structure databases

    ProteinModelPortaliQ16706.
    SMRiQ16706. Positions 126-1072.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 55CytoplasmicSequence Analysis
    Topological domaini27 – 11441118LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei6 – 2621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 38 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0383.
    HOGENOMiHOG000293253.
    HOVERGENiHBG052390.
    InParanoidiQ16706.
    KOiK01231.
    OMAiPRMTMSK.
    OrthoDBiEOG7JQBMJ.
    PhylomeDBiQ16706.
    TreeFamiTF313152.

    Family and domain databases

    Gene3Di1.20.1270.50. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.110.10. 1 hit.
    InterProiIPR011013. Gal_mutarotase_SF_dom.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR013780. Glyco_hydro_13_b.
    IPR027291. Glyco_hydro_38/57_N.
    IPR011682. Glyco_hydro_38_C.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view]
    PfamiPF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 1 hit.
    PF07748. Glyco_hydro_38C. 1 hit.
    [Graphical view]
    SMARTiSM00872. Alpha-mann_mid. 1 hit.
    [Graphical view]
    SUPFAMiSSF74650. SSF74650. 1 hit.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q16706-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLSRQFTVF GSAIFCVVIF SLYLMLDRGH LDYPRNPRRE GSFPQGQLSM     50
    LQEKIDHLER LLAENNEIIS NIRDSVINLS ESVEDGPKSS QSNFSQGAGS 100
    HLLPSQLSLS VDTADCLFAS QSGSHNSDVQ MLDVYSLISF DNPDGGVWKQ 150
    GFDITYESNE WDTEPLQVFV VPHSHNDPGW LKTFNDYFRD KTQYIFNNMV 200
    LKLKEDSRRK FIWSEISYLS KWWDIIDIQK KDAVKSLIEN GQLEIVTGGW 250
    VMPDEATPHY FALIDQLIEG HQWLENNIGV KPRSGWAIDP FGHSPTMAYL 300
    LNRAGLSHML IQRVHYAVKK HFALHKTLEF FWRQNWDLGS VTDILCHMMP 350
    FYSYDIPHTC GPDPKICCQF DFKRLPGGRF GCPWGVPPET IHPGNVQSRA 400
    RMLLDQYRKK SKLFRTKVLL APLGDDFRYC EYTEWDLQFK NYQQLFDYMN 450
    SQSKFKVKIQ FGTLSDFFDA LDKADETQRD KGQSMFPVLS GDFFTYADRD 500
    DHYWSGYFTS RPFYKRMDRI MESHLRAAEI LYYFALRQAH KYKINKFLSS 550
    SLYTALTEAR RNLGLFQHHD AITGTAKDWV VVDYGTRLFH SLMVLEKIIG 600
    NSAFLLILKD KLTYDSYSPD TFLEMDLKQK SQDSLPQKNI IRLSAEPRYL 650
    VVYNPLEQDR ISLVSVYVSS PTVQVFSASG KPVEVQVSAV WDTANTISET 700
    AYEISFRAHI PPLGLKVYKI LESASSNSHL ADYVLYKNKV EDSGIFTIKN 750
    MINTEEGITL ENSFVLLRFD QTGLMKQMMT KEDGKHHEVN VQFSWYGTTI 800
    KRDKSGAYLF LPDGNAKPYV YTTPPFVRVT HGRIYSEVTC FFDHVTHRVR 850
    LYHIQGIEGQ SVEVSNIVDI RKVYNREIAM KISSDIKSQN RFYTDLNGYQ 900
    IQPRMTLSKL PLQANVYPMT TMAYIQDAKH RLTLLSAQSL GVSSLNSGQI 950
    EVIMDRRLMQ DDNRGLEQGI QDNKITANLF RILLEKRSAV NTEEEKKSVS 1000
    YPSLLSHITS SLMNHPVIPM ANKFSSPTLE LQGEFSPLQS SLPCDIHLVN 1050
    LRTIQSKVGN GHSNEAALIL HRKGFDCRFS SKGTGLFCST TQGKILVQKL 1100
    LNKFIVESLT PSSLSLMHSP PGTQNISEIN LSPMEISTFR IQLR 1144
    Length:1,144
    Mass (Da):131,141
    Last modified:May 1, 2007 - v2
    Checksum:iA1814C63D1CE4FAC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti608 – 6081L → G in AAC50302. (PubMed:8524845)Curated
    Sequence conflicti1026 – 10261Missing in BAA10017. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U31520 mRNA. Translation: AAC50302.1.
    D63998 mRNA. Translation: BAA10017.1.
    CCDSiCCDS34209.1.
    RefSeqiNP_002363.2. NM_002372.3.
    UniGeneiHs.432822.

    Genome annotation databases

    EnsembliENST00000261483; ENSP00000261483; ENSG00000112893.
    GeneIDi4124.
    KEGGihsa:4124.
    UCSCiuc003kou.1. human.

    Polymorphism databases

    DMDMi146345453.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U31520 mRNA. Translation: AAC50302.1 .
    D63998 mRNA. Translation: BAA10017.1 .
    CCDSi CCDS34209.1.
    RefSeqi NP_002363.2. NM_002372.3.
    UniGenei Hs.432822.

    3D structure databases

    ProteinModelPortali Q16706.
    SMRi Q16706. Positions 126-1072.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110297. 4 interactions.
    IntActi Q16706. 1 interaction.
    MINTi MINT-2807655.
    STRINGi 9606.ENSP00000261483.

    Chemistry

    BindingDBi Q16706.
    ChEMBLi CHEMBL4056.

    Protein family/group databases

    CAZyi GH38. Glycoside Hydrolase Family 38.

    PTM databases

    PhosphoSitei Q16706.

    Polymorphism databases

    DMDMi 146345453.

    Proteomic databases

    MaxQBi Q16706.
    PaxDbi Q16706.
    PRIDEi Q16706.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000261483 ; ENSP00000261483 ; ENSG00000112893 .
    GeneIDi 4124.
    KEGGi hsa:4124.
    UCSCi uc003kou.1. human.

    Organism-specific databases

    CTDi 4124.
    GeneCardsi GC05P109053.
    H-InvDB HIX0005076.
    HGNCi HGNC:6824. MAN2A1.
    HPAi CAB022377.
    HPA026896.
    MIMi 154582. gene.
    neXtProti NX_Q16706.
    PharmGKBi PA30573.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0383.
    HOGENOMi HOG000293253.
    HOVERGENi HBG052390.
    InParanoidi Q16706.
    KOi K01231.
    OMAi PRMTMSK.
    OrthoDBi EOG7JQBMJ.
    PhylomeDBi Q16706.
    TreeFami TF313152.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BioCyci MetaCyc:HS03629-MONOMER.
    Reactomei REACT_25302. Reactions specific to the complex N-glycan synthesis pathway.

    Miscellaneous databases

    GeneWikii MAN2A1.
    GenomeRNAii 4124.
    NextBioi 16192.
    PROi Q16706.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q16706.
    Bgeei Q16706.
    CleanExi HS_MAN2A1.
    Genevestigatori Q16706.

    Family and domain databases

    Gene3Di 1.20.1270.50. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.110.10. 1 hit.
    InterProi IPR011013. Gal_mutarotase_SF_dom.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR013780. Glyco_hydro_13_b.
    IPR027291. Glyco_hydro_38/57_N.
    IPR011682. Glyco_hydro_38_C.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view ]
    Pfami PF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 1 hit.
    PF07748. Glyco_hydro_38C. 1 hit.
    [Graphical view ]
    SMARTi SM00872. Alpha-mann_mid. 1 hit.
    [Graphical view ]
    SUPFAMi SSF74650. SSF74650. 1 hit.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase IIx isozyme."
      Misago M., Liao Y.-F., Kudo S., Eto S., Mattei M.-G., Moremen K.W., Fukuda M.N.
      Proc. Natl. Acad. Sci. U.S.A. 92:11766-11770(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "Molecular cloning and sequence analysis of human Golgi mannosidase II."
      Misumi Y., Hashimoto C., Sohoda M., Ogata S., Ikehara Y.
      Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    3. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
      Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
      J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-78 AND ASN-1125.
      Tissue: Plasma.
    4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMA2A1_HUMAN
    AccessioniPrimary (citable) accession number: Q16706
    Secondary accession number(s): Q16767
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    3. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    4. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3