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Q16706

- MA2A1_HUMAN

UniProt

Q16706 - MA2A1_HUMAN

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Protein

Alpha-mannosidase 2

Gene

MAN2A1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.

Catalytic activityi

Hydrolysis of the terminal (1->3)- and (1->6)-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man5(GlcNAc)3.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi175 – 1751ZincBy similarity
Metal bindingi177 – 1771ZincBy similarity
Active sitei289 – 2891NucleophileBy similarity
Metal bindingi289 – 2891ZincBy similarity
Metal bindingi569 – 5691ZincBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. hydrolase activity, hydrolyzing N-glycosyl compounds Source: Ensembl
  3. mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity Source: ProtInc
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. cellular protein metabolic process Source: Reactome
  2. in utero embryonic development Source: Ensembl
  3. liver development Source: Ensembl
  4. lung alveolus development Source: Ensembl
  5. mannose metabolic process Source: InterPro
  6. mitochondrion organization Source: Ensembl
  7. N-glycan processing Source: Ensembl
  8. positive regulation of neurogenesis Source: Ensembl
  9. post-translational protein modification Source: Reactome
  10. protein N-linked glycosylation via asparagine Source: Reactome
  11. respiratory gaseous exchange Source: Ensembl
  12. retina morphogenesis in camera-type eye Source: Ensembl
  13. vacuole organization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:HS03629-MONOMER.
ReactomeiREACT_25302. Reactions specific to the complex N-glycan synthesis pathway.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase 2 (EC:3.2.1.114)
Alternative name(s):
Golgi alpha-mannosidase II
Short name:
AMan II
Short name:
Man II
Mannosidase alpha class 2A member 1
Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Gene namesi
Name:MAN2A1
Synonyms:MANA2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:6824. MAN2A1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55CytoplasmicSequence Analysis
Transmembranei6 – 2621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini27 – 11441118LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cis-Golgi network Source: Ensembl
  2. extracellular vesicular exosome Source: UniProtKB
  3. Golgi membrane Source: Reactome
  4. integral component of membrane Source: UniProtKB-KW
  5. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30573.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11441144Alpha-mannosidase 2PRO_0000206902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi78 – 781N-linked (GlcNAc...)1 Publication
Modified residuei80 – 801PhosphoserineBy similarity
Modified residuei82 – 821PhosphoserineBy similarity
Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1125 – 11251N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ16706.
PaxDbiQ16706.
PRIDEiQ16706.

PTM databases

PhosphoSiteiQ16706.

Expressioni

Gene expression databases

BgeeiQ16706.
CleanExiHS_MAN2A1.
ExpressionAtlasiQ16706. baseline and differential.
GenevestigatoriQ16706.

Organism-specific databases

HPAiCAB022377.
HPA026896.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

BioGridi110297. 8 interactions.
IntActiQ16706. 1 interaction.
MINTiMINT-2807655.
STRINGi9606.ENSP00000261483.

Structurei

3D structure databases

ProteinModelPortaliQ16706.
SMRiQ16706. Positions 126-1072.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0383.
GeneTreeiENSGT00510000046304.
HOGENOMiHOG000293253.
HOVERGENiHBG052390.
InParanoidiQ16706.
KOiK01231.
OMAiPRMTMSK.
OrthoDBiEOG7JQBMJ.
PhylomeDBiQ16706.
TreeFamiTF313152.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Q16706-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLSRQFTVF GSAIFCVVIF SLYLMLDRGH LDYPRNPRRE GSFPQGQLSM
60 70 80 90 100
LQEKIDHLER LLAENNEIIS NIRDSVINLS ESVEDGPKSS QSNFSQGAGS
110 120 130 140 150
HLLPSQLSLS VDTADCLFAS QSGSHNSDVQ MLDVYSLISF DNPDGGVWKQ
160 170 180 190 200
GFDITYESNE WDTEPLQVFV VPHSHNDPGW LKTFNDYFRD KTQYIFNNMV
210 220 230 240 250
LKLKEDSRRK FIWSEISYLS KWWDIIDIQK KDAVKSLIEN GQLEIVTGGW
260 270 280 290 300
VMPDEATPHY FALIDQLIEG HQWLENNIGV KPRSGWAIDP FGHSPTMAYL
310 320 330 340 350
LNRAGLSHML IQRVHYAVKK HFALHKTLEF FWRQNWDLGS VTDILCHMMP
360 370 380 390 400
FYSYDIPHTC GPDPKICCQF DFKRLPGGRF GCPWGVPPET IHPGNVQSRA
410 420 430 440 450
RMLLDQYRKK SKLFRTKVLL APLGDDFRYC EYTEWDLQFK NYQQLFDYMN
460 470 480 490 500
SQSKFKVKIQ FGTLSDFFDA LDKADETQRD KGQSMFPVLS GDFFTYADRD
510 520 530 540 550
DHYWSGYFTS RPFYKRMDRI MESHLRAAEI LYYFALRQAH KYKINKFLSS
560 570 580 590 600
SLYTALTEAR RNLGLFQHHD AITGTAKDWV VVDYGTRLFH SLMVLEKIIG
610 620 630 640 650
NSAFLLILKD KLTYDSYSPD TFLEMDLKQK SQDSLPQKNI IRLSAEPRYL
660 670 680 690 700
VVYNPLEQDR ISLVSVYVSS PTVQVFSASG KPVEVQVSAV WDTANTISET
710 720 730 740 750
AYEISFRAHI PPLGLKVYKI LESASSNSHL ADYVLYKNKV EDSGIFTIKN
760 770 780 790 800
MINTEEGITL ENSFVLLRFD QTGLMKQMMT KEDGKHHEVN VQFSWYGTTI
810 820 830 840 850
KRDKSGAYLF LPDGNAKPYV YTTPPFVRVT HGRIYSEVTC FFDHVTHRVR
860 870 880 890 900
LYHIQGIEGQ SVEVSNIVDI RKVYNREIAM KISSDIKSQN RFYTDLNGYQ
910 920 930 940 950
IQPRMTLSKL PLQANVYPMT TMAYIQDAKH RLTLLSAQSL GVSSLNSGQI
960 970 980 990 1000
EVIMDRRLMQ DDNRGLEQGI QDNKITANLF RILLEKRSAV NTEEEKKSVS
1010 1020 1030 1040 1050
YPSLLSHITS SLMNHPVIPM ANKFSSPTLE LQGEFSPLQS SLPCDIHLVN
1060 1070 1080 1090 1100
LRTIQSKVGN GHSNEAALIL HRKGFDCRFS SKGTGLFCST TQGKILVQKL
1110 1120 1130 1140
LNKFIVESLT PSSLSLMHSP PGTQNISEIN LSPMEISTFR IQLR
Length:1,144
Mass (Da):131,141
Last modified:May 1, 2007 - v2
Checksum:iA1814C63D1CE4FAC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti608 – 6081L → G in AAC50302. (PubMed:8524845)Curated
Sequence conflicti1026 – 10261Missing in BAA10017. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31520 mRNA. Translation: AAC50302.1.
D63998 mRNA. Translation: BAA10017.1.
CCDSiCCDS34209.1.
RefSeqiNP_002363.2. NM_002372.3.
UniGeneiHs.432822.

Genome annotation databases

EnsembliENST00000261483; ENSP00000261483; ENSG00000112893.
GeneIDi4124.
KEGGihsa:4124.
UCSCiuc003kou.1. human.

Polymorphism databases

DMDMi146345453.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31520 mRNA. Translation: AAC50302.1 .
D63998 mRNA. Translation: BAA10017.1 .
CCDSi CCDS34209.1.
RefSeqi NP_002363.2. NM_002372.3.
UniGenei Hs.432822.

3D structure databases

ProteinModelPortali Q16706.
SMRi Q16706. Positions 126-1072.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 110297. 8 interactions.
IntActi Q16706. 1 interaction.
MINTi MINT-2807655.
STRINGi 9606.ENSP00000261483.

Chemistry

BindingDBi Q16706.
ChEMBLi CHEMBL4056.

Protein family/group databases

CAZyi GH38. Glycoside Hydrolase Family 38.

PTM databases

PhosphoSitei Q16706.

Polymorphism databases

DMDMi 146345453.

Proteomic databases

MaxQBi Q16706.
PaxDbi Q16706.
PRIDEi Q16706.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000261483 ; ENSP00000261483 ; ENSG00000112893 .
GeneIDi 4124.
KEGGi hsa:4124.
UCSCi uc003kou.1. human.

Organism-specific databases

CTDi 4124.
GeneCardsi GC05P109053.
H-InvDB HIX0005076.
HGNCi HGNC:6824. MAN2A1.
HPAi CAB022377.
HPA026896.
MIMi 154582. gene.
neXtProti NX_Q16706.
PharmGKBi PA30573.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0383.
GeneTreei ENSGT00510000046304.
HOGENOMi HOG000293253.
HOVERGENi HBG052390.
InParanoidi Q16706.
KOi K01231.
OMAi PRMTMSK.
OrthoDBi EOG7JQBMJ.
PhylomeDBi Q16706.
TreeFami TF313152.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BioCyci MetaCyc:HS03629-MONOMER.
Reactomei REACT_25302. Reactions specific to the complex N-glycan synthesis pathway.

Miscellaneous databases

ChiTaRSi MAN2A1. human.
GeneWikii MAN2A1.
GenomeRNAii 4124.
NextBioi 16192.
PROi Q16706.
SOURCEi Search...

Gene expression databases

Bgeei Q16706.
CleanExi HS_MAN2A1.
ExpressionAtlasi Q16706. baseline and differential.
Genevestigatori Q16706.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase IIx isozyme."
    Misago M., Liao Y.-F., Kudo S., Eto S., Mattei M.-G., Moremen K.W., Fukuda M.N.
    Proc. Natl. Acad. Sci. U.S.A. 92:11766-11770(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Molecular cloning and sequence analysis of human Golgi mannosidase II."
    Misumi Y., Hashimoto C., Sohoda M., Ogata S., Ikehara Y.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
    Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
    J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-78 AND ASN-1125.
    Tissue: Plasma.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMA2A1_HUMAN
AccessioniPrimary (citable) accession number: Q16706
Secondary accession number(s): Q16767
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 2007
Last modified: November 26, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3