Q166L9 (SYM_ROSDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	RD1_2516

Organism

Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans) [Complete proteome] [HAMAP]

Taxonomic identifier

375451 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Roseobacter › Roseobacter denitrificans

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Methionine--tRNA ligase HAMAP-Rule MF_00098		PRO_0000331891
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP-Rule MF_00098
	Motif	346 – 350	5	"KMSKS" region HAMAP-Rule MF_00098
Sites
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Binding site	349	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q166L9 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: C0BF6D5B7A4C0DBD	FASTA	572	64,345
	10 20 30 40 50 60 MERHLITSAI PYINGIKHLG NLVGSQLPAD LYARYLRARG HEVLFLCATD EHGTPAELAA 70 80 90 100 110 120 AKAGKPVDEY CAEMHAIQSE IAKGFRLSFD HFGRSSSAQN HRLTQHFAGK LDEANLIDEV 130 140 150 160 170 180 IEKQVYSHAD GRFLPDRYIE GTCPNCAYDK ARGDQCENCT KQLDPTDLIE PRSAISGSTD 190 200 210 220 230 240 LEVRETKHLY LKQSVLKDQL DAWIDSKTDW PVLTTSIAKK WLHDGDGLRD RGITRDLDWG 250 260 270 280 290 300 IPVKKGTQDW PGMEGKVFYV WFDAPIEYIA CASEWAEANN LEQSDWERWW RTDKGADDVR 310 320 330 340 350 360 YTQFMGKDNV PFHTLSFPAT LLGSGEPWKM VDHLKSFNYL NYDGGQFSTS QGRGIFMDQA 370 380 390 400 410 420 LELLPADYWR WWLLSHAPEN SDSEFTWENF QSSVNKDLAD VLGNFVSRIT KFCAAKFGSE 430 440 450 460 470 480 VPAGGAYGSA ELELLTKLDE SLTRYQDYMG AMEVRKSAQE LRAIWASGNE YLQTVAPWSV 490 500 510 520 530 540 FKTDPETAAM QTRLGLNLAL LFGVLSSPFV PDASEKILKC LGHPSARWPD TCTDFVTSMK 550 560 570 PGDPFEVPEV LFAKLTDEDR ATYSAKFSGT QS

References

[1]

"The complete genome sequence of Roseobacter denitrificans reveals a mixotrophic rather than photosynthetic metabolism." Swingley W.D., Sadekar S., Mastrian S.D., Matthies H.J., Hao J., Ramos H., Acharya C.R., Conrad A.L., Taylor H.L., Dejesa L.C., Shah M.K., O'Huallachain M.E., Lince M.T., Blankenship R.E., Beatty J.T., Touchman J.W. J. Bacteriol. 189:683-690(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33942 / OCh 114.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000362 Genomic DNA. Translation: ABG32074.1.
RefSeq	YP_682760.1. NC_008209.1.
3D structure databases
ProteinModelPortal	Q166L9.
ModBase	Search...
Protein-protein interaction databases
STRING	375451.RD1_2516.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABG32074; ABG32074; RD1_2516.
GeneID	4195264.
KEGG	rde:RD1_2516.
PATRIC	23363055. VBIRosDen86677_2408.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0143.
HOGENOM	HOG000200402.
KO	K01874.
OMA	LTQHFAG.
ProtClustDB	PRK12268.
Enzyme and pathway databases
BioCyc	RDEN375451:GJIZ-2371-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits.
HAMAP	MF_00098. Met_tRNA_synth_type1.
InterPro	IPR023458. Met-tRNA_ligase_1. IPR014758. Met-tRNA_synth. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_ROSDO

Accession

Primary (citable) accession number: Q166L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	July 25, 2006
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program