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Q16674 (MIA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Melanoma-derived growth regulatory protein
Alternative name(s):
Melanoma inhibitory activity protein
Gene names
Name:MIA
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length131 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

Subunit structure

Interacts with FASLG. Interacts with TMIGD2. Ref.9 Ref.10

Subcellular location

Secreted.

Tissue specificity

All malignant melanoma cell lines tested and infrequently in glioma cell lines.

Post-translational modification

May possess two intramolecular disulfide bonds.

Sequence similarities

Belongs to the MIA/OTOR family.

Contains 1 SH3 domain.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DomainSH3 domain
Signal
   Molecular functionGrowth factor
   PTMDisulfide bond
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcell proliferation

Traceable author statement Ref.1. Source: ProtInc

   Cellular_componentextracellular space

Traceable author statement Ref.1. Source: ProtInc

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q16674-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q16674-2)

Also known as: MIA-splice;

The sequence of this isoform differs from the canonical sequence as follows:
     43-131: HPISMAVALQ...KTDKWDFYCQ → RSGRLLWRSGCSPGLFPQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Ref.8
Chain25 – 131107Melanoma-derived growth regulatory protein
PRO_0000019028

Regions

Domain43 – 11371SH3

Amino acid modifications

Disulfide bond36 ↔ 41
Disulfide bond59 ↔ 130

Natural variations

Alternative sequence43 – 13189HPISM…DFYCQ → RSGRLLWRSGCSPGLFPQ in isoform 2.
VSP_044450

Secondary structure

................... 131
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 4D3BB30BD6008BDC

FASTA13114,509
        10         20         30         40         50         60 
MARSLVCLGV IILLSAFSGP GVRGGPMPKL ADRKLCADQE CSHPISMAVA LQDYMAPDCR 

        70         80         90        100        110        120 
FLTIHRGQVV YVFSKLKGRG RLFWGGSVQG DYYGDLAARL GYFPSSIVRE DQTLKPGKVD 

       130 
VKTDKWDFYC Q 

« Hide

Isoform 2 (MIA-splice) [UniParc].

Checksum: 0DF07A3AE87FD5C5
Show »

FASTA606,361

References

« Hide 'large scale' references
[1]"Cloning of a novel malignant melanoma-derived growth-regulatory protein, MIA."
Blesch A., Bosserhoff A.-K., Apfel R., Behl C., Hessdoerfer B., Schmitt A., Jachimczak P., Lottspeich F., Buettner R., Bogdahn U.
Cancer Res. 54:5695-5701(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
[2]"Structure and promoter analysis of the gene encoding the human melanoma-inhibiting protein MIA."
Bosserhoff A.-K., Hein R., Bogdahn U., Buettner R.
J. Biol. Chem. 271:490-495(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Placenta.
[3]"Cloning and characterization of the expression pattern of a novel splice product MIA (splice) of malignant melanoma-derived growth-inhibiting activity (MIA/CD-RAP)."
Hau P., Wise P., Bosserhoff A.K., Blesch A., Jachimczak P., Tschertner I., Bogdahn U., Apfel R.
J. Invest. Dermatol. 119:562-569(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING.
[4]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Skin.
[8]"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z., Henzel W.J.
Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-39.
[9]"Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening."
Voss M., Lettau M., Janssen O.
BMC Immunol. 10:53-53(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FASLG.
[10]"Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis."
Rahimi N., Rezazadeh K., Mahoney J.E., Hartsough E., Meyer R.D.
Mol. Biol. Cell 23:1646-1656(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TMIGD2.
[11]"Structure of melanoma inhibitory activity protein, a member of a recently identified family of secreted proteins."
Lougheed J.C., Holton J.M., Alber T., Bazan J.F., Handel T.M.
Proc. Natl. Acad. Sci. U.S.A. 98:5515-5520(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.39 ANGSTROMS) OF 25-131.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X75450 mRNA. Translation: CAA53203.1.
X84707 Genomic DNA. Translation: CAA59195.1.
BT007044 mRNA. Translation: AAP35693.1.
CR541648 mRNA. Translation: CAG46449.1.
CH471126 Genomic DNA. Translation: EAW56997.1.
BC005910 mRNA. Translation: AAH05910.1.
CCDSCCDS12566.1. [Q16674-1]
PIRI38019.
RefSeqNP_001189482.1. NM_001202553.1. [Q16674-1]
NP_006524.1. NM_006533.3. [Q16674-1]
UniGeneHs.646364.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1HJDNMR-A31-131[»]
1I1JX-ray1.39A/B25-131[»]
1K0XNMR-A25-131[»]
ProteinModelPortalQ16674.
SMRQ16674. Positions 25-131.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113834. 3 interactions.
IntActQ16674. 1 interaction.
STRING9606.ENSP00000263369.

PTM databases

PhosphoSiteQ16674.

Polymorphism databases

DMDM2498559.

Proteomic databases

PaxDbQ16674.
PRIDEQ16674.

Protocols and materials databases

DNASU8190.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000263369; ENSP00000263369; ENSG00000261857. [Q16674-1]
ENST00000594436; ENSP00000470129; ENSG00000261857. [Q16674-1]
ENST00000597784; ENSP00000469499; ENSG00000261857. [Q16674-1]
GeneID8190.
KEGGhsa:8190.
UCSCuc002opb.4. human. [Q16674-1]

Organism-specific databases

CTD8190.
GeneCardsGC19P041283.
HGNCHGNC:7076. MIA.
HPAHPA042369.
MIM601340. gene.
neXtProtNX_Q16674.
PharmGKBPA30800.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG43576.
HOGENOMHOG000063668.
HOVERGENHBG000503.
InParanoidQ16674.
KOK16655.
OMADCRFIPI.
PhylomeDBQ16674.
TreeFamTF332724.

Gene expression databases

BgeeQ16674.
CleanExHS_MIA.
GenevestigatorQ16674.

Family and domain databases

InterProIPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamPF07653. SH3_2. 1 hit.
[Graphical view]
SMARTSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
PROSITEPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ16674.
GeneWikiMelanoma_inhibitory_activity.
GenomeRNAi8190.
NextBio30882.
PROQ16674.
SOURCESearch...

Entry information

Entry nameMIA_HUMAN
AccessionPrimary (citable) accession number: Q16674
Secondary accession number(s): Q6FHV3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM