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Protein

Melanoma-derived growth regulatory protein

Gene

MIA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

GO - Biological processi

  1. cell proliferation Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Growth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Melanoma-derived growth regulatory protein
Alternative name(s):
Melanoma inhibitory activity protein
Gene namesi
Name:MIA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:7076. MIA.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30800.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationAdd
BLAST
Chaini25 – 131107Melanoma-derived growth regulatory proteinPRO_0000019028Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi36 ↔ 41
Disulfide bondi59 ↔ 130

Post-translational modificationi

May possess two intramolecular disulfide bonds.

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ16674.
PRIDEiQ16674.

PTM databases

PhosphoSiteiQ16674.

Expressioni

Tissue specificityi

All malignant melanoma cell lines tested and infrequently in glioma cell lines.

Gene expression databases

BgeeiQ16674.
CleanExiHS_MIA.
ExpressionAtlasiQ16674. baseline.
GenevestigatoriQ16674.

Organism-specific databases

HPAiHPA042369.

Interactioni

Subunit structurei

Interacts with FASLG. Interacts with TMIGD2.2 Publications

Protein-protein interaction databases

BioGridi113834. 3 interactions.
IntActiQ16674. 1 interaction.
STRINGi9606.ENSP00000263369.

Structurei

Secondary structure

1
131
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi32 – 387Combined sources
Beta strandi45 – 528Combined sources
Beta strandi57 – 615Combined sources
Beta strandi69 – 768Combined sources
Helixi78 – 803Combined sources
Beta strandi83 – 897Combined sources
Beta strandi101 – 1044Combined sources
Helixi105 – 1073Combined sources
Beta strandi108 – 1136Combined sources
Beta strandi119 – 1224Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HJDNMR-A31-131[»]
1I1JX-ray1.39A/B25-131[»]
1K0XNMR-A25-131[»]
ProteinModelPortaliQ16674.
SMRiQ16674. Positions 25-131.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ16674.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 11371SH3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MIA/OTOR family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain, Signal

Phylogenomic databases

eggNOGiNOG43576.
GeneTreeiENSGT00390000010535.
HOGENOMiHOG000063668.
HOVERGENiHBG000503.
InParanoidiQ16674.
KOiK16655.
OMAiFIPIRQG.
PhylomeDBiQ16674.
TreeFamiTF332724.

Family and domain databases

InterProiIPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q16674-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARSLVCLGV IILLSAFSGP GVRGGPMPKL ADRKLCADQE CSHPISMAVA
60 70 80 90 100
LQDYMAPDCR FLTIHRGQVV YVFSKLKGRG RLFWGGSVQG DYYGDLAARL
110 120 130
GYFPSSIVRE DQTLKPGKVD VKTDKWDFYC Q
Length:131
Mass (Da):14,509
Last modified:November 1, 1997 - v1
Checksum:i4D3BB30BD6008BDC
GO
Isoform 2 (identifier: Q16674-2) [UniParc]FASTAAdd to Basket

Also known as: MIA-splice

The sequence of this isoform differs from the canonical sequence as follows:
     43-131: HPISMAVALQ...KTDKWDFYCQ → RSGRLLWRSGCSPGLFPQ

Show »
Length:60
Mass (Da):6,361
Checksum:i0DF07A3AE87FD5C5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei43 – 13189HPISM…DFYCQ → RSGRLLWRSGCSPGLFPQ in isoform 2. 1 PublicationVSP_044450Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75450 mRNA. Translation: CAA53203.1.
X84707 Genomic DNA. Translation: CAA59195.1.
BT007044 mRNA. Translation: AAP35693.1.
CR541648 mRNA. Translation: CAG46449.1.
CH471126 Genomic DNA. Translation: EAW56997.1.
BC005910 mRNA. Translation: AAH05910.1.
CCDSiCCDS12566.1. [Q16674-1]
PIRiI38019.
RefSeqiNP_001189482.1. NM_001202553.1. [Q16674-1]
NP_006524.1. NM_006533.3. [Q16674-1]
UniGeneiHs.646364.

Genome annotation databases

EnsembliENST00000263369; ENSP00000263369; ENSG00000261857. [Q16674-1]
ENST00000594436; ENSP00000470129; ENSG00000261857. [Q16674-1]
ENST00000597784; ENSP00000469499; ENSG00000261857. [Q16674-1]
GeneIDi8190.
KEGGihsa:8190.
UCSCiuc002opb.4. human. [Q16674-1]

Polymorphism databases

DMDMi2498559.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75450 mRNA. Translation: CAA53203.1.
X84707 Genomic DNA. Translation: CAA59195.1.
BT007044 mRNA. Translation: AAP35693.1.
CR541648 mRNA. Translation: CAG46449.1.
CH471126 Genomic DNA. Translation: EAW56997.1.
BC005910 mRNA. Translation: AAH05910.1.
CCDSiCCDS12566.1. [Q16674-1]
PIRiI38019.
RefSeqiNP_001189482.1. NM_001202553.1. [Q16674-1]
NP_006524.1. NM_006533.3. [Q16674-1]
UniGeneiHs.646364.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HJDNMR-A31-131[»]
1I1JX-ray1.39A/B25-131[»]
1K0XNMR-A25-131[»]
ProteinModelPortaliQ16674.
SMRiQ16674. Positions 25-131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113834. 3 interactions.
IntActiQ16674. 1 interaction.
STRINGi9606.ENSP00000263369.

PTM databases

PhosphoSiteiQ16674.

Polymorphism databases

DMDMi2498559.

Proteomic databases

PaxDbiQ16674.
PRIDEiQ16674.

Protocols and materials databases

DNASUi8190.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263369; ENSP00000263369; ENSG00000261857. [Q16674-1]
ENST00000594436; ENSP00000470129; ENSG00000261857. [Q16674-1]
ENST00000597784; ENSP00000469499; ENSG00000261857. [Q16674-1]
GeneIDi8190.
KEGGihsa:8190.
UCSCiuc002opb.4. human. [Q16674-1]

Organism-specific databases

CTDi8190.
GeneCardsiGC19P041283.
HGNCiHGNC:7076. MIA.
HPAiHPA042369.
MIMi601340. gene.
neXtProtiNX_Q16674.
PharmGKBiPA30800.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG43576.
GeneTreeiENSGT00390000010535.
HOGENOMiHOG000063668.
HOVERGENiHBG000503.
InParanoidiQ16674.
KOiK16655.
OMAiFIPIRQG.
PhylomeDBiQ16674.
TreeFamiTF332724.

Miscellaneous databases

EvolutionaryTraceiQ16674.
GeneWikiiMelanoma_inhibitory_activity.
GenomeRNAii8190.
NextBioi30882.
PROiQ16674.
SOURCEiSearch...

Gene expression databases

BgeeiQ16674.
CleanExiHS_MIA.
ExpressionAtlasiQ16674. baseline.
GenevestigatoriQ16674.

Family and domain databases

InterProiIPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a novel malignant melanoma-derived growth-regulatory protein, MIA."
    Blesch A., Bosserhoff A.-K., Apfel R., Behl C., Hessdoerfer B., Schmitt A., Jachimczak P., Lottspeich F., Buettner R., Bogdahn U.
    Cancer Res. 54:5695-5701(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
  2. "Structure and promoter analysis of the gene encoding the human melanoma-inhibiting protein MIA."
    Bosserhoff A.-K., Hein R., Bogdahn U., Buettner R.
    J. Biol. Chem. 271:490-495(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Placenta.
  3. "Cloning and characterization of the expression pattern of a novel splice product MIA (splice) of malignant melanoma-derived growth-inhibiting activity (MIA/CD-RAP)."
    Hau P., Wise P., Bosserhoff A.K., Blesch A., Jachimczak P., Tschertner I., Bogdahn U., Apfel R.
    J. Invest. Dermatol. 119:562-569(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING.
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Skin.
  8. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
    Zhang Z., Henzel W.J.
    Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-39.
  9. "Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening."
    Voss M., Lettau M., Janssen O.
    BMC Immunol. 10:53-53(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FASLG.
  10. "Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis."
    Rahimi N., Rezazadeh K., Mahoney J.E., Hartsough E., Meyer R.D.
    Mol. Biol. Cell 23:1646-1656(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TMIGD2.
  11. "Structure of melanoma inhibitory activity protein, a member of a recently identified family of secreted proteins."
    Lougheed J.C., Holton J.M., Alber T., Bazan J.F., Handel T.M.
    Proc. Natl. Acad. Sci. U.S.A. 98:5515-5520(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.39 ANGSTROMS) OF 25-131.

Entry informationi

Entry nameiMIA_HUMAN
AccessioniPrimary (citable) accession number: Q16674
Secondary accession number(s): Q6FHV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.