Q16674 (MIA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Melanoma-derived growth regulatory protein

Alternative name(s):
Melanoma inhibitory activity protein

Gene names

Name:

MIA

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
Subunit structure	Interacts with FASLG. Interacts with TMIGD2. Ref.9 Ref.10
Subcellular location	Secreted.
Tissue specificity	All malignant melanoma cell lines tested and infrequently in glioma cell lines.
Post-translational modification	May possess two intramolecular disulfide bonds.
Sequence similarities	Belongs to the MIA/OTOR family. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	SH3 domain Signal
Molecular function	Growth factor
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell proliferation Traceable author statement Ref.1. Source: ProtInc
Cellular_component	extracellular space Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q16674-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q16674-2)

Also known as: MIA-splice;

The sequence of this isoform differs from the canonical sequence as follows:
     43-131: HPISMAVALQ...KTDKWDFYCQ → RSGRLLWRSGCSPGLFPQ

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Ref.8

Chain

25 – 131

107

Melanoma-derived growth regulatory protein

PRO_0000019028

Regions

Domain

43 – 113

71

SH3

Amino acid modifications

Disulfide bond

36 ↔ 41

Disulfide bond

59 ↔ 130

Natural variations

Alternative sequence

43 – 131

89

HPISM…DFYCQ → RSGRLLWRSGCSPGLFPQ in isoform 2.

VSP_044450

Secondary structure

1

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131

Helix Strand Turn

Details...

Beta strand

32 – 38

7

Beta strand

45 – 52

8

Beta strand

57 – 61

5

Beta strand

69 – 76

8

Helix

78 – 80

3

Beta strand

83 – 89

7

Beta strand

101 – 104

4

Helix

105 – 107

3

Beta strand

108 – 113

6

Beta strand

119 – 122

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 4D3BB30BD6008BDC	FASTA	131	14,509
	10 20 30 40 50 60 MARSLVCLGV IILLSAFSGP GVRGGPMPKL ADRKLCADQE CSHPISMAVA LQDYMAPDCR 70 80 90 100 110 120 FLTIHRGQVV YVFSKLKGRG RLFWGGSVQG DYYGDLAARL GYFPSSIVRE DQTLKPGKVD 130 VKTDKWDFYC Q
	Isoform 2 (MIA-splice) [UniParc]. Checksum: 0DF07A3AE87FD5C5	FASTA	60	6,361
	10 20 30 40 50 60 MARSLVCLGV IILLSAFSGP GVRGGPMPKL ADRKLCADQE CSRSGRLLWR SGCSPGLFPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a novel malignant melanoma-derived growth-regulatory protein, MIA." Blesch A., Bosserhoff A.-K., Apfel R., Behl C., Hessdoerfer B., Schmitt A., Jachimczak P., Lottspeich F., Buettner R., Bogdahn U. Cancer Res. 54:5695-5701(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
[2]	"Structure and promoter analysis of the gene encoding the human melanoma-inhibiting protein MIA." Bosserhoff A.-K., Hein R., Bogdahn U., Buettner R. J. Biol. Chem. 271:490-495(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[3]	"Cloning and characterization of the expression pattern of a novel splice product MIA (splice) of malignant melanoma-derived growth-inhibiting activity (MIA/CD-RAP)." Hau P., Wise P., Bosserhoff A.K., Blesch A., Jachimczak P., Tschertner I., Bogdahn U., Apfel R. J. Invest. Dermatol. 119:562-569(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Skin.
[8]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-39.
[9]	"Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening." Voss M., Lettau M., Janssen O. BMC Immunol. 10:53-53(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FASLG.
[10]	"Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis." Rahimi N., Rezazadeh K., Mahoney J.E., Hartsough E., Meyer R.D. Mol. Biol. Cell 23:1646-1656(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TMIGD2.
[11]	"Structure of melanoma inhibitory activity protein, a member of a recently identified family of secreted proteins." Lougheed J.C., Holton J.M., Alber T., Bazan J.F., Handel T.M. Proc. Natl. Acad. Sci. U.S.A. 98:5515-5520(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.39 ANGSTROMS) OF 25-131.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X75450 mRNA. Translation: CAA53203.1.
X84707 Genomic DNA. Translation: CAA59195.1.
BT007044 mRNA. Translation: AAP35693.1.
CR541648 mRNA. Translation: CAG46449.1.
CH471126 Genomic DNA. Translation: EAW56997.1.
BC005910 mRNA. Translation: AAH05910.1.

IPI

IPI00003448.

PIR

I38019.

RefSeq

NP_001189482.1. NM_001202553.1.
NP_006524.1. NM_006533.3.

UniGene

Hs.646364.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HJD	NMR	-	A	31-131	[»]
1I1J	X-ray	1.39	A/B	25-131	[»]
1K0X	NMR	-	A	25-131	[»]

ProteinModelPortal

Q16674.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q16674. 1 interaction.

STRING

9606.ENSP00000263369.

PTM databases

PhosphoSite

Q16674.

Polymorphism databases

DMDM

2498559.

Proteomic databases

PaxDb

Q16674.

PRIDE

Q16674.

Protocols and materials databases

DNASU

8190.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000263369; ENSP00000263369; ENSG00000261857.
ENST00000594436; ENSP00000470129; ENSG00000261857.
ENST00000597784; ENSP00000469499; ENSG00000261857.

GeneID

8190.

KEGG

hsa:8190.

UCSC

uc002opb.4. human.

Organism-specific databases

CTD

8190.

GeneCards

GC19P041283.

HGNC

HGNC:7076. MIA.

HPA

HPA042369.

MIM

601340. gene.

neXtProt

NX_Q16674.

PharmGKB

PA30800.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG43576.

HOGENOM

HOG000063668.

HOVERGEN

HBG000503.

InParanoid

Q16674.

KO

K16655.

OrthoDB

EOG40ZR04.

PhylomeDB

Q16674.

Gene expression databases

Bgee

Q16674.

CleanEx

HS_MIA.

Genevestigator

Q16674.

GermOnline

ENSG00000167578. Homo sapiens.

Family and domain databases

InterPro

IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]

Pfam

PF07653. SH3_2. 1 hit.
[Graphical view]

SMART

SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF50044. SH3. 1 hit.

PROSITE

PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q16674.

GenomeRNAi

8190.

NextBio

30882.

SOURCE

Search...

Entry information

Entry name

MIA_HUMAN

Accession

Primary (citable) accession number: Q16674
Secondary accession number(s): Q6FHV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.