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Q16674

- MIA_HUMAN

UniProt

Q16674 - MIA_HUMAN

Protein

Melanoma-derived growth regulatory protein

Gene

MIA

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

    GO - Biological processi

    1. cell proliferation Source: ProtInc

    Keywords - Molecular functioni

    Growth factor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Melanoma-derived growth regulatory protein
    Alternative name(s):
    Melanoma inhibitory activity protein
    Gene namesi
    Name:MIA
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:7076. MIA.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: ProtInc

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30800.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 24241 PublicationAdd
    BLAST
    Chaini25 – 131107Melanoma-derived growth regulatory proteinPRO_0000019028Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi36 ↔ 41
    Disulfide bondi59 ↔ 130

    Post-translational modificationi

    May possess two intramolecular disulfide bonds.

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ16674.
    PRIDEiQ16674.

    PTM databases

    PhosphoSiteiQ16674.

    Expressioni

    Tissue specificityi

    All malignant melanoma cell lines tested and infrequently in glioma cell lines.

    Gene expression databases

    BgeeiQ16674.
    CleanExiHS_MIA.
    GenevestigatoriQ16674.

    Organism-specific databases

    HPAiHPA042369.

    Interactioni

    Subunit structurei

    Interacts with FASLG. Interacts with TMIGD2.2 Publications

    Protein-protein interaction databases

    BioGridi113834. 3 interactions.
    IntActiQ16674. 1 interaction.
    STRINGi9606.ENSP00000263369.

    Structurei

    Secondary structure

    1
    131
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi32 – 387
    Beta strandi45 – 528
    Beta strandi57 – 615
    Beta strandi69 – 768
    Helixi78 – 803
    Beta strandi83 – 897
    Beta strandi101 – 1044
    Helixi105 – 1073
    Beta strandi108 – 1136
    Beta strandi119 – 1224

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1HJDNMR-A31-131[»]
    1I1JX-ray1.39A/B25-131[»]
    1K0XNMR-A25-131[»]
    ProteinModelPortaliQ16674.
    SMRiQ16674. Positions 25-131.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ16674.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 11371SH3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MIA/OTOR family.Curated
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain, Signal

    Phylogenomic databases

    eggNOGiNOG43576.
    HOGENOMiHOG000063668.
    HOVERGENiHBG000503.
    InParanoidiQ16674.
    KOiK16655.
    OMAiDCRFIPI.
    PhylomeDBiQ16674.
    TreeFamiTF332724.

    Family and domain databases

    InterProiIPR011511. SH3_2.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF07653. SH3_2. 1 hit.
    [Graphical view]
    SMARTiSM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS50002. SH3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q16674-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MARSLVCLGV IILLSAFSGP GVRGGPMPKL ADRKLCADQE CSHPISMAVA    50
    LQDYMAPDCR FLTIHRGQVV YVFSKLKGRG RLFWGGSVQG DYYGDLAARL 100
    GYFPSSIVRE DQTLKPGKVD VKTDKWDFYC Q 131
    Length:131
    Mass (Da):14,509
    Last modified:November 1, 1997 - v1
    Checksum:i4D3BB30BD6008BDC
    GO
    Isoform 2 (identifier: Q16674-2) [UniParc]FASTAAdd to Basket

    Also known as: MIA-splice

    The sequence of this isoform differs from the canonical sequence as follows:
         43-131: HPISMAVALQ...KTDKWDFYCQ → RSGRLLWRSGCSPGLFPQ

    Show »
    Length:60
    Mass (Da):6,361
    Checksum:i0DF07A3AE87FD5C5
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei43 – 13189HPISM…DFYCQ → RSGRLLWRSGCSPGLFPQ in isoform 2. 1 PublicationVSP_044450Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75450 mRNA. Translation: CAA53203.1.
    X84707 Genomic DNA. Translation: CAA59195.1.
    BT007044 mRNA. Translation: AAP35693.1.
    CR541648 mRNA. Translation: CAG46449.1.
    CH471126 Genomic DNA. Translation: EAW56997.1.
    BC005910 mRNA. Translation: AAH05910.1.
    CCDSiCCDS12566.1. [Q16674-1]
    PIRiI38019.
    RefSeqiNP_001189482.1. NM_001202553.1. [Q16674-1]
    NP_006524.1. NM_006533.3. [Q16674-1]
    UniGeneiHs.646364.

    Genome annotation databases

    EnsembliENST00000263369; ENSP00000263369; ENSG00000261857. [Q16674-1]
    ENST00000594436; ENSP00000470129; ENSG00000261857. [Q16674-1]
    ENST00000597784; ENSP00000469499; ENSG00000261857. [Q16674-1]
    GeneIDi8190.
    KEGGihsa:8190.
    UCSCiuc002opb.4. human. [Q16674-1]

    Polymorphism databases

    DMDMi2498559.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75450 mRNA. Translation: CAA53203.1 .
    X84707 Genomic DNA. Translation: CAA59195.1 .
    BT007044 mRNA. Translation: AAP35693.1 .
    CR541648 mRNA. Translation: CAG46449.1 .
    CH471126 Genomic DNA. Translation: EAW56997.1 .
    BC005910 mRNA. Translation: AAH05910.1 .
    CCDSi CCDS12566.1. [Q16674-1 ]
    PIRi I38019.
    RefSeqi NP_001189482.1. NM_001202553.1. [Q16674-1 ]
    NP_006524.1. NM_006533.3. [Q16674-1 ]
    UniGenei Hs.646364.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1HJD NMR - A 31-131 [» ]
    1I1J X-ray 1.39 A/B 25-131 [» ]
    1K0X NMR - A 25-131 [» ]
    ProteinModelPortali Q16674.
    SMRi Q16674. Positions 25-131.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113834. 3 interactions.
    IntActi Q16674. 1 interaction.
    STRINGi 9606.ENSP00000263369.

    PTM databases

    PhosphoSitei Q16674.

    Polymorphism databases

    DMDMi 2498559.

    Proteomic databases

    PaxDbi Q16674.
    PRIDEi Q16674.

    Protocols and materials databases

    DNASUi 8190.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000263369 ; ENSP00000263369 ; ENSG00000261857 . [Q16674-1 ]
    ENST00000594436 ; ENSP00000470129 ; ENSG00000261857 . [Q16674-1 ]
    ENST00000597784 ; ENSP00000469499 ; ENSG00000261857 . [Q16674-1 ]
    GeneIDi 8190.
    KEGGi hsa:8190.
    UCSCi uc002opb.4. human. [Q16674-1 ]

    Organism-specific databases

    CTDi 8190.
    GeneCardsi GC19P041283.
    HGNCi HGNC:7076. MIA.
    HPAi HPA042369.
    MIMi 601340. gene.
    neXtProti NX_Q16674.
    PharmGKBi PA30800.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG43576.
    HOGENOMi HOG000063668.
    HOVERGENi HBG000503.
    InParanoidi Q16674.
    KOi K16655.
    OMAi DCRFIPI.
    PhylomeDBi Q16674.
    TreeFami TF332724.

    Miscellaneous databases

    EvolutionaryTracei Q16674.
    GeneWikii Melanoma_inhibitory_activity.
    GenomeRNAii 8190.
    NextBioi 30882.
    PROi Q16674.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q16674.
    CleanExi HS_MIA.
    Genevestigatori Q16674.

    Family and domain databases

    InterProi IPR011511. SH3_2.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF07653. SH3_2. 1 hit.
    [Graphical view ]
    SMARTi SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a novel malignant melanoma-derived growth-regulatory protein, MIA."
      Blesch A., Bosserhoff A.-K., Apfel R., Behl C., Hessdoerfer B., Schmitt A., Jachimczak P., Lottspeich F., Buettner R., Bogdahn U.
      Cancer Res. 54:5695-5701(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
    2. "Structure and promoter analysis of the gene encoding the human melanoma-inhibiting protein MIA."
      Bosserhoff A.-K., Hein R., Bogdahn U., Buettner R.
      J. Biol. Chem. 271:490-495(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Placenta.
    3. "Cloning and characterization of the expression pattern of a novel splice product MIA (splice) of malignant melanoma-derived growth-inhibiting activity (MIA/CD-RAP)."
      Hau P., Wise P., Bosserhoff A.K., Blesch A., Jachimczak P., Tschertner I., Bogdahn U., Apfel R.
      J. Invest. Dermatol. 119:562-569(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING.
    4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Skin.
    8. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
      Zhang Z., Henzel W.J.
      Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-39.
    9. "Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening."
      Voss M., Lettau M., Janssen O.
      BMC Immunol. 10:53-53(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FASLG.
    10. "Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis."
      Rahimi N., Rezazadeh K., Mahoney J.E., Hartsough E., Meyer R.D.
      Mol. Biol. Cell 23:1646-1656(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TMIGD2.
    11. "Structure of melanoma inhibitory activity protein, a member of a recently identified family of secreted proteins."
      Lougheed J.C., Holton J.M., Alber T., Bazan J.F., Handel T.M.
      Proc. Natl. Acad. Sci. U.S.A. 98:5515-5520(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.39 ANGSTROMS) OF 25-131.

    Entry informationi

    Entry nameiMIA_HUMAN
    AccessioniPrimary (citable) accession number: Q16674
    Secondary accession number(s): Q6FHV3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3