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Protein

Nuclear respiratory factor 1

Gene

NRF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi109 – 305Add BLAST197

GO - Molecular functioni

GO - Biological processi

  • generation of precursor metabolites and energy Source: ProtInc
  • mitochondrion organization Source: Reactome
  • multicellular organism development Source: GO_Central
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • regulation of transcription from RNA polymerase II promoter Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000106459-MONOMER.
ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-2151201. Transcriptional activation of mitochondrial biogenesis.
SIGNORiQ16656.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear respiratory factor 1
Short name:
NRF-1
Alternative name(s):
Alpha palindromic-binding protein
Short name:
Alpha-pal
Gene namesi
Name:NRF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:7996. NRF1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • nucleoplasm Source: Reactome
  • nucleus Source: AgBase
  • transcription factor complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4899.
OpenTargetsiENSG00000106459.
PharmGKBiPA31775.

Polymorphism and mutation databases

BioMutaiNRF1.
DMDMi12643732.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001002081 – 503Nuclear respiratory factor 1Add BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39Phosphoserine; by CK21 Publication1
Modified residuei44Phosphoserine; by CK21 Publication1
Modified residuei46Phosphoserine; by CK21 Publication1
Modified residuei47Phosphoserine; by CK21 Publication1
Modified residuei52Phosphoserine; by CK21 Publication1

Post-translational modificationi

Phosphorylation enhances DNA binding.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ16656.
MaxQBiQ16656.
PaxDbiQ16656.
PeptideAtlasiQ16656.
PRIDEiQ16656.

PTM databases

iPTMnetiQ16656.
PhosphoSitePlusiQ16656.

Expressioni

Tissue specificityi

Ubiquitously expressed with strongest expression in skeletal muscle.

Gene expression databases

BgeeiENSG00000106459.
CleanExiHS_NRF1.
ExpressionAtlasiQ16656. baseline and differential.
GenevisibleiQ16656. HS.

Organism-specific databases

HPAiHPA029329.

Interactioni

Subunit structurei

Homodimer. Binds DNA as a dimer. Interacts with PPRC1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FHL2J3KNW44EBI-11742836,EBI-11994324
FHL2Q141923EBI-2547810,EBI-701903
POGZQ7Z3K36EBI-11742836,EBI-1389308
SP4Q024463EBI-2547810,EBI-10198587
TRAF2Q129333EBI-2547810,EBI-355744

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110955. 20 interactors.
DIPiDIP-56958N.
IntActiQ16656. 20 interactors.
STRINGi9606.ENSP00000223190.

Structurei

3D structure databases

ProteinModelPortaliQ16656.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 78DimerizationAdd BLAST78
Regioni301 – 476Required for transcriptional activationAdd BLAST176

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi88 – 116Nuclear localization signalAdd BLAST29

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi41 – 66Asp/Glu-rich (acidic)Add BLAST26
Compositional biasi80 – 86Poly-Ala7

Sequence similaritiesi

Belongs to the NRF1/Ewg family.Curated

Phylogenomic databases

eggNOGiENOG410IDYK. Eukaryota.
ENOG410XNU7. LUCA.
GeneTreeiENSGT00390000006835.
HOGENOMiHOG000273898.
HOVERGENiHBG003062.
InParanoidiQ16656.
KOiK11831.
OMAiQTEHMTT.
PhylomeDBiQ16656.
TreeFamiTF105308.

Family and domain databases

InterProiIPR019526. Nrf1_activation-bd.
IPR019525. Nrf1_NLS/DNA-bd_dimer.
[Graphical view]
PfamiPF10492. Nrf1_activ_bdg. 1 hit.
PF10491. Nrf1_DNA-bind. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q16656-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEHGVTQTE HMATIEAHAV AQQVQQVHVA TYTEHSMLSA DEDSPSSPED
60 70 80 90 100
TSYDDSDILN STAADEVTAH LAAAGPVGMA AAAAVATGKK RKRPHVFESN
110 120 130 140 150
PSIRKRQQTR LLRKLRATLD EYTTRVGQQA IVLCISPSKP NPVFKVFGAA
160 170 180 190 200
PLENVVRKYK SMILEDLESA LAEHAPAPQE VNSELPPLTI DGIPVSVDKM
210 220 230 240 250
TQAQLRAFIP EMLKYSTGRG KPGWGKESCK PIWWPEDIPW ANVRSDVRTE
260 270 280 290 300
EQKQRVSWTQ ALRTIVKNCY KQHGREDLLY AFEDQQTQTQ ATATHSIAHL
310 320 330 340 350
VPSQTVVQTF SNPDGTVSLI QVGTGATVAT LADASELPTT VTVAQVNYSA
360 370 380 390 400
VADGEVEQNW ATLQGGEMTI QTTQASEATQ AVASLAEAAV AASQEMQQGA
410 420 430 440 450
TVTMALNSEA AAHAVATLAE ATLQGGGQIV LSGETAAAVG ALTGVQDANG
460 470 480 490 500
LVQIPVSMYQ TVVTSLAQGN GPVQVAMAPV TTRISDSAVT MDGQAVEVVT

LEQ
Length:503
Mass (Da):53,541
Last modified:November 1, 1996 - v1
Checksum:iF31C358278371929
GO
Isoform Short (identifier: Q16656-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     256-321: Missing.

Show »
Length:437
Mass (Da):46,157
Checksum:iD7B62154D8D015B2
GO
Isoform 3 (identifier: Q16656-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-161: Missing.

Note: No experimental confirmation available.
Show »
Length:342
Mass (Da):36,051
Checksum:i5130FF5034DC7362
GO
Isoform 4 (identifier: Q16656-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     449-449: N → NGLFMADRAGRKWILTDKAT

Show »
Length:522
Mass (Da):55,674
Checksum:i580AC14584586AEF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti322V → VV in AAA79013 (PubMed:7629110).Curated1
Sequence conflicti403T → A in BAG56867 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0543301 – 161Missing in isoform 3. 1 PublicationAdd BLAST161
Alternative sequenceiVSP_003598256 – 321Missing in isoform Short. CuratedAdd BLAST66
Alternative sequenceiVSP_054331449N → NGLFMADRAGRKWILTDKAT in isoform 4. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22454 mRNA. Translation: AAA16918.1.
U02683 mRNA. Translation: AAA21647.1.
U18383
, U18375, U18376, U18377, U18378, U18379, U18380, U18381, U18382 Genomic DNA. Translation: AAA79013.1.
EU159452 mRNA. Translation: ABV90872.1.
AK290891 mRNA. Translation: BAF83580.1.
AK293351 mRNA. Translation: BAG56867.1.
AC078846 Genomic DNA. No translation available.
AC084864 Genomic DNA. No translation available.
CH236950 Genomic DNA. Translation: EAL24100.1.
CH471070 Genomic DNA. Translation: EAW83728.1.
BC016925 mRNA. Translation: AAH16925.1.
CCDSiCCDS5813.2. [Q16656-1]
CCDS78273.1. [Q16656-4]
PIRiA54868.
RefSeqiNP_001035199.1. NM_001040110.1. [Q16656-1]
NP_001280092.1. NM_001293163.1. [Q16656-4]
NP_001280093.1. NM_001293164.1. [Q16656-3]
NP_005002.3. NM_005011.4. [Q16656-1]
UniGeneiHs.654363.

Genome annotation databases

EnsembliENST00000223190; ENSP00000223190; ENSG00000106459. [Q16656-1]
ENST00000311967; ENSP00000309826; ENSG00000106459. [Q16656-4]
ENST00000353868; ENSP00000342351; ENSG00000106459. [Q16656-4]
ENST00000393230; ENSP00000376922; ENSG00000106459. [Q16656-1]
ENST00000393232; ENSP00000376924; ENSG00000106459. [Q16656-1]
GeneIDi4899.
KEGGihsa:4899.
UCSCiuc003voz.4. human. [Q16656-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22454 mRNA. Translation: AAA16918.1.
U02683 mRNA. Translation: AAA21647.1.
U18383
, U18375, U18376, U18377, U18378, U18379, U18380, U18381, U18382 Genomic DNA. Translation: AAA79013.1.
EU159452 mRNA. Translation: ABV90872.1.
AK290891 mRNA. Translation: BAF83580.1.
AK293351 mRNA. Translation: BAG56867.1.
AC078846 Genomic DNA. No translation available.
AC084864 Genomic DNA. No translation available.
CH236950 Genomic DNA. Translation: EAL24100.1.
CH471070 Genomic DNA. Translation: EAW83728.1.
BC016925 mRNA. Translation: AAH16925.1.
CCDSiCCDS5813.2. [Q16656-1]
CCDS78273.1. [Q16656-4]
PIRiA54868.
RefSeqiNP_001035199.1. NM_001040110.1. [Q16656-1]
NP_001280092.1. NM_001293163.1. [Q16656-4]
NP_001280093.1. NM_001293164.1. [Q16656-3]
NP_005002.3. NM_005011.4. [Q16656-1]
UniGeneiHs.654363.

3D structure databases

ProteinModelPortaliQ16656.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110955. 20 interactors.
DIPiDIP-56958N.
IntActiQ16656. 20 interactors.
STRINGi9606.ENSP00000223190.

PTM databases

iPTMnetiQ16656.
PhosphoSitePlusiQ16656.

Polymorphism and mutation databases

BioMutaiNRF1.
DMDMi12643732.

Proteomic databases

EPDiQ16656.
MaxQBiQ16656.
PaxDbiQ16656.
PeptideAtlasiQ16656.
PRIDEiQ16656.

Protocols and materials databases

DNASUi4899.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000223190; ENSP00000223190; ENSG00000106459. [Q16656-1]
ENST00000311967; ENSP00000309826; ENSG00000106459. [Q16656-4]
ENST00000353868; ENSP00000342351; ENSG00000106459. [Q16656-4]
ENST00000393230; ENSP00000376922; ENSG00000106459. [Q16656-1]
ENST00000393232; ENSP00000376924; ENSG00000106459. [Q16656-1]
GeneIDi4899.
KEGGihsa:4899.
UCSCiuc003voz.4. human. [Q16656-1]

Organism-specific databases

CTDi4899.
DisGeNETi4899.
GeneCardsiNRF1.
HGNCiHGNC:7996. NRF1.
HPAiHPA029329.
MIMi600879. gene.
neXtProtiNX_Q16656.
OpenTargetsiENSG00000106459.
PharmGKBiPA31775.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IDYK. Eukaryota.
ENOG410XNU7. LUCA.
GeneTreeiENSGT00390000006835.
HOGENOMiHOG000273898.
HOVERGENiHBG003062.
InParanoidiQ16656.
KOiK11831.
OMAiQTEHMTT.
PhylomeDBiQ16656.
TreeFamiTF105308.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000106459-MONOMER.
ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-2151201. Transcriptional activation of mitochondrial biogenesis.
SIGNORiQ16656.

Miscellaneous databases

ChiTaRSiNRF1. human.
GeneWikiiNRF1.
GenomeRNAii4899.
PROiQ16656.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000106459.
CleanExiHS_NRF1.
ExpressionAtlasiQ16656. baseline and differential.
GenevisibleiQ16656. HS.

Family and domain databases

InterProiIPR019526. Nrf1_activation-bd.
IPR019525. Nrf1_NLS/DNA-bd_dimer.
[Graphical view]
PfamiPF10492. Nrf1_activ_bdg. 1 hit.
PF10491. Nrf1_DNA-bind. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNRF1_HUMAN
AccessioniPrimary (citable) accession number: Q16656
Secondary accession number(s): A8K4C6
, B4DDV6, Q15305, Q96AN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.