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Protein

Nuclear respiratory factor 1

Gene

NRF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi109 – 305Add BLAST197

GO - Molecular functioni

GO - Biological processi

  • generation of precursor metabolites and energy Source: ProtInc
  • mitochondrion organization Source: Reactome
  • positive regulation of transcription by RNA polymerase II Source: NTNU_SB
  • regulation of transcription by RNA polymerase II Source: ProtInc

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-2151201 Transcriptional activation of mitochondrial biogenesis
SIGNORiQ16656

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear respiratory factor 1
Short name:
NRF-1
Alternative name(s):
Alpha palindromic-binding protein
Short name:
Alpha-pal
Gene namesi
Name:NRF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000106459.14
HGNCiHGNC:7996 NRF1
MIMi600879 gene
neXtProtiNX_Q16656

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4899
OpenTargetsiENSG00000106459
PharmGKBiPA31775

Polymorphism and mutation databases

BioMutaiNRF1
DMDMi12643732

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001002081 – 503Nuclear respiratory factor 1Add BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39Phosphoserine; by CK21 Publication1
Modified residuei44Phosphoserine; by CK21 Publication1
Modified residuei46Phosphoserine; by CK21 Publication1
Modified residuei47Phosphoserine; by CK21 Publication1
Modified residuei52Phosphoserine; by CK21 Publication1
Cross-linki139Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Phosphorylation enhances DNA binding.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ16656
MaxQBiQ16656
PaxDbiQ16656
PeptideAtlasiQ16656
PRIDEiQ16656

PTM databases

iPTMnetiQ16656
PhosphoSitePlusiQ16656

Expressioni

Tissue specificityi

Ubiquitously expressed with strongest expression in skeletal muscle.

Gene expression databases

BgeeiENSG00000106459
CleanExiHS_NRF1
ExpressionAtlasiQ16656 baseline and differential
GenevisibleiQ16656 HS

Organism-specific databases

HPAiHPA029329

Interactioni

Subunit structurei

Homodimer. Binds DNA as a dimer. Interacts with PPRC1.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110955, 21 interactors
DIPiDIP-56958N
IntActiQ16656, 31 interactors
MINTiQ16656
STRINGi9606.ENSP00000223190

Structurei

3D structure databases

ProteinModelPortaliQ16656
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 78DimerizationAdd BLAST78
Regioni301 – 476Required for transcriptional activationAdd BLAST176

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi88 – 116Nuclear localization signalAdd BLAST29

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi41 – 66Asp/Glu-rich (acidic)Add BLAST26
Compositional biasi80 – 86Poly-Ala7

Sequence similaritiesi

Belongs to the NRF1/Ewg family.Curated

Phylogenomic databases

eggNOGiENOG410IDYK Eukaryota
ENOG410XNU7 LUCA
GeneTreeiENSGT00390000006835
HOGENOMiHOG000273898
HOVERGENiHBG003062
InParanoidiQ16656
KOiK11831
OMAiTIHTSQG
PhylomeDBiQ16656
TreeFamiTF105308

Family and domain databases

InterProiView protein in InterPro
IPR019526 Nrf1_activation-bd
IPR019525 Nrf1_NLS/DNA-bd_dimer
PfamiView protein in Pfam
PF10492 Nrf1_activ_bdg, 1 hit
PF10491 Nrf1_DNA-bind, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q16656-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEHGVTQTE HMATIEAHAV AQQVQQVHVA TYTEHSMLSA DEDSPSSPED
60 70 80 90 100
TSYDDSDILN STAADEVTAH LAAAGPVGMA AAAAVATGKK RKRPHVFESN
110 120 130 140 150
PSIRKRQQTR LLRKLRATLD EYTTRVGQQA IVLCISPSKP NPVFKVFGAA
160 170 180 190 200
PLENVVRKYK SMILEDLESA LAEHAPAPQE VNSELPPLTI DGIPVSVDKM
210 220 230 240 250
TQAQLRAFIP EMLKYSTGRG KPGWGKESCK PIWWPEDIPW ANVRSDVRTE
260 270 280 290 300
EQKQRVSWTQ ALRTIVKNCY KQHGREDLLY AFEDQQTQTQ ATATHSIAHL
310 320 330 340 350
VPSQTVVQTF SNPDGTVSLI QVGTGATVAT LADASELPTT VTVAQVNYSA
360 370 380 390 400
VADGEVEQNW ATLQGGEMTI QTTQASEATQ AVASLAEAAV AASQEMQQGA
410 420 430 440 450
TVTMALNSEA AAHAVATLAE ATLQGGGQIV LSGETAAAVG ALTGVQDANG
460 470 480 490 500
LVQIPVSMYQ TVVTSLAQGN GPVQVAMAPV TTRISDSAVT MDGQAVEVVT

LEQ
Length:503
Mass (Da):53,541
Last modified:November 1, 1996 - v1
Checksum:iF31C358278371929
GO
Isoform Short (identifier: Q16656-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     256-321: Missing.

Show »
Length:437
Mass (Da):46,157
Checksum:iD7B62154D8D015B2
GO
Isoform 3 (identifier: Q16656-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-161: Missing.

Note: No experimental confirmation available.
Show »
Length:342
Mass (Da):36,051
Checksum:i5130FF5034DC7362
GO
Isoform 4 (identifier: Q16656-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     449-449: N → NGLFMADRAGRKWILTDKAT

Show »
Length:522
Mass (Da):55,674
Checksum:i580AC14584586AEF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti322V → VV in AAA79013 (PubMed:7629110).Curated1
Sequence conflicti403T → A in BAG56867 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0543301 – 161Missing in isoform 3. 1 PublicationAdd BLAST161
Alternative sequenceiVSP_003598256 – 321Missing in isoform Short. CuratedAdd BLAST66
Alternative sequenceiVSP_054331449N → NGLFMADRAGRKWILTDKAT in isoform 4. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22454 mRNA Translation: AAA16918.1
U02683 mRNA Translation: AAA21647.1
U18383
, U18375, U18376, U18377, U18378, U18379, U18380, U18381, U18382 Genomic DNA Translation: AAA79013.1
EU159452 mRNA Translation: ABV90872.1
AK290891 mRNA Translation: BAF83580.1
AK293351 mRNA Translation: BAG56867.1
AC078846 Genomic DNA No translation available.
AC084864 Genomic DNA No translation available.
CH236950 Genomic DNA Translation: EAL24100.1
CH471070 Genomic DNA Translation: EAW83728.1
BC016925 mRNA Translation: AAH16925.1
CCDSiCCDS5813.2 [Q16656-1]
CCDS78273.1 [Q16656-4]
PIRiA54868
RefSeqiNP_001035199.1, NM_001040110.1 [Q16656-1]
NP_001280092.1, NM_001293163.1 [Q16656-4]
NP_001280093.1, NM_001293164.1 [Q16656-3]
NP_005002.3, NM_005011.4 [Q16656-1]
UniGeneiHs.654363

Genome annotation databases

EnsembliENST00000223190; ENSP00000223190; ENSG00000106459 [Q16656-1]
ENST00000311967; ENSP00000309826; ENSG00000106459 [Q16656-4]
ENST00000353868; ENSP00000342351; ENSG00000106459 [Q16656-4]
ENST00000393230; ENSP00000376922; ENSG00000106459 [Q16656-1]
ENST00000393232; ENSP00000376924; ENSG00000106459 [Q16656-1]
GeneIDi4899
KEGGihsa:4899
UCSCiuc003voz.4 human [Q16656-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNRF1_HUMAN
AccessioniPrimary (citable) accession number: Q16656
Secondary accession number(s): A8K4C6
, B4DDV6, Q15305, Q96AN2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health