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Protein

Prostasin

Gene

PRSS8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Charge relay system1
Active sitei134Charge relay system1
Active sitei238Charge relay system1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciZFISH:ENSG00000052344-MONOMER.
BRENDAi3.4.21.B6. 2681.
ReactomeiR-HSA-6809371. Formation of the cornified envelope.

Protein family/group databases

MEROPSiS01.159.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostasin (EC:3.4.21.-)
Alternative name(s):
Channel-activating protease 1
Short name:
CAP1
Serine protease 8
Cleaved into the following 2 chains:
Gene namesi
Name:PRSS8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:9491. PRSS8.

Subcellular locationi

Chain Prostasin light chain :
Chain Prostasin heavy chain :

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei320 – 340HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • anchored component of plasma membrane Source: GO_Central
  • extracellular exosome Source: UniProtKB
  • extracellular region Source: ProtInc
  • extracellular space Source: UniProtKB
  • extrinsic component of plasma membrane Source: GO_Central
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi5652.
OpenTargetsiENSG00000052344.
PharmGKBiPA33840.

Chemistry databases

ChEMBLiCHEMBL5610.
GuidetoPHARMACOLOGYi2400.

Polymorphism and mutation databases

BioMutaiPRSS8.
DMDMi2833277.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
PropeptideiPRO_000002802730 – 32Activation peptide3
ChainiPRO_000024051133 – 343ProstasinAdd BLAST311
ChainiPRO_000002802833 – 44Prostasin light chainAdd BLAST12
ChainiPRO_000002802945 – 322Prostasin heavy chainAdd BLAST278
PropeptideiPRO_0000028030323 – 343Add BLAST21

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37 ↔ 154Interchain (between light and heavy chains)PROSITE-ProRule annotation
Disulfide bondi70 ↔ 86PROSITE-ProRule annotation1 Publication
Glycosylationi159N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi168 ↔ 244PROSITE-ProRule annotation1 Publication
Disulfide bondi201 ↔ 223PROSITE-ProRule annotation1 Publication
Disulfide bondi234 ↔ 262PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiQ16651.
MaxQBiQ16651.
PaxDbiQ16651.
PeptideAtlasiQ16651.
PRIDEiQ16651.

Expressioni

Tissue specificityi

Found in prostate, liver, salivary gland, kidney, lung, pancreas, colon, bronchus and renal proximal tubular cells. In the prostate gland it may be synthesized in epithelial cells, secreted into the ducts, and excreted into the seminal fluid.

Gene expression databases

BgeeiENSG00000052344.
CleanExiHS_PRSS8.
ExpressionAtlasiQ16651. baseline and differential.
GenevisibleiQ16651. HS.

Organism-specific databases

HPAiHPA030436.

Interactioni

Subunit structurei

Heterodimer of two chains, light and heavy, held by a disulfide bond.1 Publication

Protein-protein interaction databases

IntActiQ16651. 1 interactor.
MINTiMINT-5000236.
STRINGi9606.ENSP00000319730.

Chemistry databases

BindingDBiQ16651.

Structurei

Secondary structure

1343
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi59 – 64Combined sources6
Beta strandi67 – 74Combined sources8
Beta strandi76 – 82Combined sources7
Helixi84 – 86Combined sources3
Helixi93 – 95Combined sources3
Beta strandi96 – 101Combined sources6
Beta strandi113 – 115Combined sources3
Beta strandi117 – 122Combined sources6
Helixi124 – 128Combined sources5
Beta strandi130 – 132Combined sources3
Beta strandi136 – 142Combined sources7
Beta strandi148 – 150Combined sources3
Beta strandi167 – 174Combined sources8
Beta strandi189 – 196Combined sources8
Helixi198 – 205Combined sources8
Turni206 – 208Combined sources3
Turni218 – 220Combined sources3
Beta strandi221 – 225Combined sources5
Beta strandi227 – 230Combined sources4
Turni235 – 239Combined sources5
Beta strandi241 – 246Combined sources6
Beta strandi249 – 256Combined sources8
Beta strandi260 – 263Combined sources4
Beta strandi269 – 273Combined sources5
Helixi274 – 277Combined sources4
Helixi278 – 288Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DFJX-ray1.45A45-289[»]
3DFLX-ray2.00A45-289[»]
3E0NX-ray1.70B45-305[»]
3E0PX-ray1.70B45-305[»]
3E16X-ray1.60B45-305[»]
3E1XX-ray1.70B45-305[»]
3FVFX-ray1.60B45-305[»]
3GYLX-ray1.30B45-305[»]
3GYMX-ray2.80A/B45-305[»]
ProteinModelPortaliQ16651.
SMRiQ16651.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ16651.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 286Peptidase S1PROSITE-ProRule annotationAdd BLAST242

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118810.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ16651.
KOiK08664.
OMAiITFSRYI.
OrthoDBiEOG091G0AH5.
PhylomeDBiQ16651.
TreeFamiTF351676.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q16651-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQKGVLGPG QLGAVAILLY LGLLRSGTGA EGAEAPCGVA PQARITGGSS
60 70 80 90 100
AVAGQWPWQV SITYEGVHVC GGSLVSEQWV LSAAHCFPSE HHKEAYEVKL
110 120 130 140 150
GAHQLDSYSE DAKVSTLKDI IPHPSYLQEG SQGDIALLQL SRPITFSRYI
160 170 180 190 200
RPICLPAANA SFPNGLHCTV TGWGHVAPSV SLLTPKPLQQ LEVPLISRET
210 220 230 240 250
CNCLYNIDAK PEEPHFVQED MVCAGYVEGG KDACQGDSGG PLSCPVEGLW
260 270 280 290 300
YLTGIVSWGD ACGARNRPGV YTLASSYASW IQSKVTELQP RVVPQTQESQ
310 320 330 340
PDSNLCGSHL AFSSAPAQGL LRPILFLPLG LALGLLSPWL SEH
Length:343
Mass (Da):36,431
Last modified:November 1, 1996 - v1
Checksum:i98DD6447F5A8C1B2
GO
Isoform 2 (identifier: Q16651-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     60-113: Missing.

Note: No experimental confirmation available.
Show »
Length:289
Mass (Da):30,519
Checksum:i4A85CFA9DE10BCDD
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05663260 – 113Missing in isoform 2. 1 PublicationAdd BLAST54

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41351 mRNA. Translation: AAC41759.1.
U33446 Genomic DNA. Translation: AAB19071.1.
AK301619 mRNA. Translation: BAG63104.1.
AC009088 Genomic DNA. No translation available.
BC001462 mRNA. Translation: AAH01462.1.
CCDSiCCDS45469.1. [Q16651-1]
PIRiA57014.
RefSeqiNP_002764.1. NM_002773.4. [Q16651-1]
UniGeneiHs.75799.

Genome annotation databases

EnsembliENST00000317508; ENSP00000319730; ENSG00000052344. [Q16651-1]
ENST00000568261; ENSP00000457750; ENSG00000052344. [Q16651-2]
GeneIDi5652.
KEGGihsa:5652.
UCSCiuc002ebc.5. human. [Q16651-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41351 mRNA. Translation: AAC41759.1.
U33446 Genomic DNA. Translation: AAB19071.1.
AK301619 mRNA. Translation: BAG63104.1.
AC009088 Genomic DNA. No translation available.
BC001462 mRNA. Translation: AAH01462.1.
CCDSiCCDS45469.1. [Q16651-1]
PIRiA57014.
RefSeqiNP_002764.1. NM_002773.4. [Q16651-1]
UniGeneiHs.75799.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DFJX-ray1.45A45-289[»]
3DFLX-ray2.00A45-289[»]
3E0NX-ray1.70B45-305[»]
3E0PX-ray1.70B45-305[»]
3E16X-ray1.60B45-305[»]
3E1XX-ray1.70B45-305[»]
3FVFX-ray1.60B45-305[»]
3GYLX-ray1.30B45-305[»]
3GYMX-ray2.80A/B45-305[»]
ProteinModelPortaliQ16651.
SMRiQ16651.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ16651. 1 interactor.
MINTiMINT-5000236.
STRINGi9606.ENSP00000319730.

Chemistry databases

BindingDBiQ16651.
ChEMBLiCHEMBL5610.
GuidetoPHARMACOLOGYi2400.

Protein family/group databases

MEROPSiS01.159.

Polymorphism and mutation databases

BioMutaiPRSS8.
DMDMi2833277.

Proteomic databases

EPDiQ16651.
MaxQBiQ16651.
PaxDbiQ16651.
PeptideAtlasiQ16651.
PRIDEiQ16651.

Protocols and materials databases

DNASUi5652.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000317508; ENSP00000319730; ENSG00000052344. [Q16651-1]
ENST00000568261; ENSP00000457750; ENSG00000052344. [Q16651-2]
GeneIDi5652.
KEGGihsa:5652.
UCSCiuc002ebc.5. human. [Q16651-1]

Organism-specific databases

CTDi5652.
DisGeNETi5652.
GeneCardsiPRSS8.
HGNCiHGNC:9491. PRSS8.
HPAiHPA030436.
MIMi600823. gene.
neXtProtiNX_Q16651.
OpenTargetsiENSG00000052344.
PharmGKBiPA33840.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118810.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ16651.
KOiK08664.
OMAiITFSRYI.
OrthoDBiEOG091G0AH5.
PhylomeDBiQ16651.
TreeFamiTF351676.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000052344-MONOMER.
BRENDAi3.4.21.B6. 2681.
ReactomeiR-HSA-6809371. Formation of the cornified envelope.

Miscellaneous databases

ChiTaRSiPRSS8. human.
EvolutionaryTraceiQ16651.
GeneWikiiPRSS8.
GenomeRNAii5652.
PROiQ16651.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000052344.
CleanExiHS_PRSS8.
ExpressionAtlasiQ16651. baseline and differential.
GenevisibleiQ16651. HS.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRSS8_HUMAN
AccessioniPrimary (citable) accession number: Q16651
Secondary accession number(s): B4DWP2, Q9UCA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.