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Protein

Nuclear factor interleukin-3-regulated protein

Gene

NFIL3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity). Represses transcriptional activity of PER1 (By similarity). Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity). Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity). Protects pro-B cells from programmed cell death (By similarity).By similarity3 Publications

GO - Molecular functioni

  • DNA binding Source: ProtInc
  • RNA polymerase II core promoter sequence-specific DNA binding Source: Ensembl
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: BHF-UCL
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: BHF-UCL
  • transcription corepressor activity Source: ProtInc
  • transcription factor activity, sequence-specific DNA binding Source: ProtInc

GO - Biological processi

  • cellular response to interleukin-4 Source: Ensembl
  • circadian rhythm Source: GO_Central
  • immune response Source: ProtInc
  • negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of gene expression Source: Ensembl
  • transcription from RNA polymerase II promoter Source: ProtInc

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processBiological rhythms, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1368108. BMAL1:CLOCK,NPAS2 activates circadian gene expression.
SignaLinkiQ16649.
SIGNORiQ16649.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor interleukin-3-regulated protein
Alternative name(s):
E4 promoter-binding protein 4
Interleukin-3 promoter transcriptional activator
Interleukin-3-binding protein 1
Transcriptional activator NF-IL3A
Gene namesi
Name:NFIL3
Synonyms:E4BP4, IL3BP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000165030.3.
HGNCiHGNC:7787. NFIL3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi330K → A: Interacts with DR1 and partially affects transcriptional repression; when associated with E-332. 1 Publication1
Mutagenesisi330K → E: Does not interact with DR1 and drastically affects transcriptional repression; when associated with E-332. 1 Publication1
Mutagenesisi332K → A: Interacts with DR1 and partially affects transcriptional repression; when associated with E-330. 1 Publication1
Mutagenesisi332K → E: Does not interact with DR1 and drastically affects transcriptional repression; when associated with E-330. 1 Publication1

Organism-specific databases

DisGeNETi4783.
OpenTargetsiENSG00000165030.
PharmGKBiPA31593.

Polymorphism and mutation databases

BioMutaiNFIL3.
DMDMi150385077.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002926671 – 462Nuclear factor interleukin-3-regulated proteinAdd BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki24Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki214Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki219Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Cross-linki219Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei301PhosphoserineCombined sources1
Cross-linki306Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki314Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki326Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki332Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki337Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki350Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei353PhosphoserineCombined sources1
Cross-linki360Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki394Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki401Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki406Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki412Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki419Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki424Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki434Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki448Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ16649.
MaxQBiQ16649.
PaxDbiQ16649.
PeptideAtlasiQ16649.
PRIDEiQ16649.

PTM databases

iPTMnetiQ16649.
PhosphoSitePlusiQ16649.

Expressioni

Tissue specificityi

Expressed in bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle.1 Publication

Inductioni

Up-regulated by PHA or TPA.1 Publication

Gene expression databases

BgeeiENSG00000165030.
CleanExiHS_NFIL3.
ExpressionAtlasiQ16649. baseline and differential.
GenevisibleiQ16649. HS.

Organism-specific databases

HPAiHPA003261.

Interactioni

Subunit structurei

Homodimer. Binds DNA as a dimer. Interacts with DR1. Interacts with PER2 and CRY2 (By similarity).By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi110855. 15 interactors.
IntActiQ16649. 18 interactors.
MINTiMINT-268122.
STRINGi9606.ENSP00000297689.

Structurei

3D structure databases

ProteinModelPortaliQ16649.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 136bZIPAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 95Basic motifAdd BLAST17
Regioni99 – 106Leucine-zipper8
Regioni299 – 363Necessary for transcriptional repression and sufficient for interaction with DR11 PublicationAdd BLAST65

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi155 – 210Ser-richAdd BLAST56

Sequence similaritiesi

Belongs to the bZIP family. NFIL3 subfamily.Curated

Phylogenomic databases

eggNOGiENOG410ISXX. Eukaryota.
ENOG410Z2VS. LUCA.
GeneTreeiENSGT00530000064087.
HOGENOMiHOG000059596.
HOVERGENiHBG105717.
InParanoidiQ16649.
KOiK09059.
OMAiAREPRDD.
OrthoDBiEOG091G06SA.
PhylomeDBiQ16649.
TreeFamiTF328374.

Family and domain databases

InterProiView protein in InterPro
IPR004827. bZIP.
IPR016743. NFIL3/E4BP4.
IPR010533. Vert_IL3-reg_TF.
PANTHERiPTHR15284:SF1. PTHR15284:SF1. 1 hit.
PfamiView protein in Pfam
PF07716. bZIP_2. 1 hit.
PF06529. Vert_IL3-reg_TF. 1 hit.
PIRSFiPIRSF019029. bZIP_E4BP4. 1 hit.
SMARTiView protein in SMART
SM00338. BRLZ. 1 hit.
PROSITEiView protein in PROSITE
PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q16649-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLRKMQTVK KEQASLDASS NVDKMMVLNS ALTEVSEDST TGEELLLSEG
60 70 80 90 100
SVGKNKSSAC RRKREFIPDE KKDAMYWEKR RKNNEAAKRS REKRRLNDLV
110 120 130 140 150
LENKLIALGE ENATLKAELL SLKLKFGLIS STAYAQEIQK LSNSTAVYFQ
160 170 180 190 200
DYQTSKSNVS SFVDEHEPSM VSSSCISVIK HSPQSSLSDV SEVSSVEHTQ
210 220 230 240 250
ESSVQGSCRS PENKFQIIKQ EPMELESYTR EPRDDRGSYT ASIYQNYMGN
260 270 280 290 300
SFSGYSHSPP LLQVNRSSSN SPRTSETDDG VVGKSSDGED EQQVPKGPIH
310 320 330 340 350
SPVELKHVHA TVVKVPEVNS SALPHKLRIK AKAMQIKVEA FDNEFEATQK
360 370 380 390 400
LSSPIDMTSK RHFELEKHSA PSMVHSSLTP FSVQVTNIQD WSLKSEHWHQ
410 420 430 440 450
KELSGKTQNS FKTGVVEMKD SGYKVSDPEN LYLKQGIANL SAEVVSLKRL
460
IATQPISASD SG
Length:462
Mass (Da):51,472
Last modified:June 26, 2007 - v2
Checksum:iD19946AAC774C3E7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44 – 45EL → DV in AAA93067 (PubMed:1620116).Curated2
Sequence conflicti44 – 45EL → DV in CAA45597 (PubMed:1620116).Curated2
Sequence conflicti44 – 45EL → DV in AAB35410 (PubMed:7565758).Curated2
Sequence conflicti149F → S in CAG46846 (Ref. 4) Curated1
Sequence conflicti273R → G in AAA93067 (PubMed:1620116).Curated1
Sequence conflicti273R → G in CAA45597 (PubMed:1620116).Curated1
Sequence conflicti273R → G in AAB35410 (PubMed:7565758).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26173 mRNA. Translation: AAA93067.1.
X64318 mRNA. Translation: CAA45597.1.
S79880 mRNA. Translation: AAB35410.1.
EF028070 Genomic DNA. Translation: ABK15691.1.
CR542049 mRNA. Translation: CAG46846.1.
AK313970 mRNA. Translation: BAG36685.1.
AL353764 Genomic DNA. No translation available.
CH471089 Genomic DNA. Translation: EAW62796.1.
BC008197 mRNA. Translation: AAH08197.1.
CCDSiCCDS6690.1.
PIRiG01804.
RefSeqiNP_001276928.1. NM_001289999.1.
NP_001276929.1. NM_001290000.1.
NP_005375.2. NM_005384.2.
XP_016870232.1. XM_017014743.1.
XP_016870233.1. XM_017014744.1.
UniGeneiHs.79334.

Genome annotation databases

EnsembliENST00000297689; ENSP00000297689; ENSG00000165030.
GeneIDi4783.
KEGGihsa:4783.
UCSCiuc004arh.3. human.

Similar proteinsi

Entry informationi

Entry nameiNFIL3_HUMAN
AccessioniPrimary (citable) accession number: Q16649
Secondary accession number(s): B2R9Y8
, Q14211, Q6FGQ8, Q96HS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: October 25, 2017
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families