Q16643 (DREB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Drebrin

Alternative name(s):
Developmentally-regulated brain protein

Gene names

Name:	DBN1
Synonyms:	D0S117E

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites, respectively.
Subunit structure	Binds F-actin.
Subcellular location	Cytoplasm.
Tissue specificity	Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas.
Sequence similarities	Contains 1 ADF-H domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Actin-binding
Molecular function	Developmental protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	actin filament organization Inferred from sequence or structural similarity. Source: UniProtKB cell communication by chemical coupling Inferred from electronic annotation. Source: Compara cell communication by electrical coupling Inferred from electronic annotation. Source: Compara maintenance of protein location in cell Inferred from electronic annotation. Source: Compara neural precursor cell proliferation Inferred from electronic annotation. Source: Compara regulation of dendrite development Non-traceable author statement PubMed 12009525. Source: UniProtKB regulation of neuronal synaptic plasticity Non-traceable author statement PubMed 12009525. Source: UniProtKB
Cellular_component	actomyosin Non-traceable author statement PubMed 12761245. Source: UniProtKB cytoplasm Non-traceable author statement PubMed 12009525. Source: UniProtKB dendrite Non-traceable author statement PubMed 12009525. Source: UniProtKB gap junction Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	actin binding Non-traceable author statement PubMed 12761245. Source: UniProtKB profilin binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q16643-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q16643-2)

The sequence of this isoform differs from the canonical sequence as follows:
     4-29: VSFSGHRLELLAAYEEVIREESAADW → HPWHGTAALASSQAWRDGRERQALVSCR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.7 Ref.8
	Chain	2 – 649	648	Drebrin		PRO_0000080008
Regions
	Domain	3 – 134	132	ADF-H
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.7 Ref.8
	Modified residue	134	1	Phosphoserine By similarity
	Modified residue	141	1	Phosphoserine Ref.15 Ref.17
	Modified residue	142	1	Phosphoserine Ref.8 Ref.13 Ref.15 Ref.17
	Modified residue	331	1	Phosphothreonine Ref.13 Ref.14
	Modified residue	335	1	Phosphothreonine Ref.13
	Modified residue	337	1	Phosphoserine Ref.13 Ref.17
	Modified residue	339	1	Phosphoserine Ref.17
	Modified residue	342	1	Phosphoserine By similarity
	Modified residue	346	1	Phosphothreonine Ref.13 Ref.14
Natural variations
	Alternative sequence	4 – 29	26	VSFSG…SAADW → HPWHGTAALASSQAWRDGRE RQALVSCR in isoform 2.		VSP_028175
	Natural variant	278	1	E → K in a breast cancer sample; somatic mutation. Ref.18		VAR_035910
	Natural variant	446	1	I → V. Ref.1 Ref.2 Ref.4 Ref.6 Corresponds to variant rs2544809 [ dbSNP | Ensembl ].		VAR_047365
	Natural variant	553	1	S → P. Ref.1 Ref.2 Ref.4 Ref.6 Corresponds to variant rs28538572 [ dbSNP | Ensembl ].		VAR_047366
	Natural variant	640	1	E → Q in a breast cancer sample; somatic mutation. Ref.18		VAR_035911

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 25, 2008. Version 4. Checksum: A7DF1AE3776C0BEA	FASTA	649	71,429
	10 20 30 40 50 60 MAGVSFSGHR LELLAAYEEV IREESAADWA LYTYEDGSDD LKLAASGEGG LQELSGHFEN 70 80 90 100 110 120 QKVMYGFCSV KDSQAALPKY VLINWVGEDV PDARKCACAS HVAKVAEFFQ GVDVIVNASS 130 140 150 160 170 180 VEDIDAGAIG QRLSNGLARL SSPVLHRLRL REDENAEPVG TTYQKTDAAV EMKRINREQF 190 200 210 220 230 240 WEQAKKEEEL RKEEERKKAL DERLRFEQER MEQERQEQEE RERRYREREQ QIEEHRRKQQ 250 260 270 280 290 300 TLEAEEAKRR LKEQSIFGDH RDEEEETHMK KSESEVEEAA AIIAQRPDNP REFFKQQERV 310 320 330 340 350 360 ASASAGSCDV PSPFNHRPGS HLDSHRRMAP TPIPTRSPSD SSTASTPVAE QIERALDEVT 370 380 390 400 410 420 SSQPPPLPPP PPPAQETQEP SPILDSEETR AAAPQAWAGP MEEPPQAQAP PRGPGSPAED 430 440 450 460 470 480 LMFMESAEQA VLAAPVEPAT ADATEIHDAA DTIETDTATA DTTVANNVPP AATSLIDLWP 490 500 510 520 530 540 GNGEGASTLQ GEPRAPTPPS GTEVTLAEVP LLDEVAPEPL LPAGEGCATL LNFDELPEPP 550 560 570 580 590 600 ATFCDPEEVE GESLAAPQTP TLPSALEELE QEQEPEPHLL TNGETTQKEG TQASEGYFSQ 610 620 630 640 SQEEEFAQSE ELCAKAPPPV FYNKPPEIDI TCWDADPVPE EEEGFEGGD
	Isoform 2 [UniParc]. Checksum: 89DA14954246C0A3	FASTA	651	71,599
	10 20 30 40 50 60 MAGHPWHGTA ALASSQAWRD GRERQALVSC RALYTYEDGS DDLKLAASGE GGLQELSGHF 70 80 90 100 110 120 ENQKVMYGFC SVKDSQAALP KYVLINWVGE DVPDARKCAC ASHVAKVAEF FQGVDVIVNA 130 140 150 160 170 180 SSVEDIDAGA IGQRLSNGLA RLSSPVLHRL RLREDENAEP VGTTYQKTDA AVEMKRINRE 190 200 210 220 230 240 QFWEQAKKEE ELRKEEERKK ALDERLRFEQ ERMEQERQEQ EERERRYRER EQQIEEHRRK 250 260 270 280 290 300 QQTLEAEEAK RRLKEQSIFG DHRDEEEETH MKKSESEVEE AAAIIAQRPD NPREFFKQQE 310 320 330 340 350 360 RVASASAGSC DVPSPFNHRP GSHLDSHRRM APTPIPTRSP SDSSTASTPV AEQIERALDE 370 380 390 400 410 420 VTSSQPPPLP PPPPPAQETQ EPSPILDSEE TRAAAPQAWA GPMEEPPQAQ APPRGPGSPA 430 440 450 460 470 480 EDLMFMESAE QAVLAAPVEP ATADATEIHD AADTIETDTA TADTTVANNV PPAATSLIDL 490 500 510 520 530 540 WPGNGEGAST LQGEPRAPTP PSGTEVTLAE VPLLDEVAPE PLLPAGEGCA TLLNFDELPE 550 560 570 580 590 600 PPATFCDPEE VEGESLAAPQ TPTLPSALEE LEQEQEPEPH LLTNGETTQK EGTQASEGYF 610 620 630 640 650 SQSQEEEFAQ SEELCAKAPP PVFYNKPPEI DITCWDADPV PEEEEGFEGG D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of cDNA encoding human drebrin E and chromosomal mapping of its gene." Toda M., Shirao T., Minoshima S., Shimizu N., Toya S., Uyemura K. Biochem. Biophys. Res. Commun. 196:468-472(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS VAL-446 AND PRO-553. Tissue: Fetal brain.
[2]	"Human drebrin: cDNA sequence, mRNA tissue distribution and chromosomal localization." Fisher L.W., McBride O.W., Filpula D., Ibaraki K., Young M.F. Neurosci. Res. Commun. 14:35-42(1994) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS VAL-446 AND PRO-553. Tissue: Osteoblast.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS VAL-446 AND PRO-553. Tissue: Testis.
[5]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS VAL-446 AND PRO-553. Tissue: Eye and Muscle.
[7]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-10, ACETYLATION AT ALA-2. Tissue: Platelet.
[8]	Bienvenut W.V., Dozynkiewicz M., Norman J.C. Submitted (JUN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-10; 43-62; 140-147; 150-165; 272-299 AND 328-390, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, PHOSPHORYLATION AT SER-142, MASS SPECTROMETRY. Tissue: Ovarian carcinoma.
[9]	Lubec G., Chen W.-Q., Sun Y. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 80-94, MASS SPECTROMETRY. Tissue: Fetal brain cortex.
[10]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[11]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[12]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Platelet.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142; THR-331; THR-335; SER-337 AND THR-346, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-331 AND THR-346, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[15]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-141 AND SER-142, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[16]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[17]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-141; SER-142; SER-337 AND SER-339, MASS SPECTROMETRY.
[18]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] LYS-278 AND GLN-640.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17530 mRNA. Translation: BAA04480.1. U00802 mRNA. Translation: AAA16256.1. AK314645 mRNA. Translation: BAG37207.1. AL110225 mRNA. Translation: CAB53683.1. AC145098 Genomic DNA. No translation available. BC000283 mRNA. Translation: AAH00283.1. BC007281 mRNA. Translation: AAH07281.1. BC007567 mRNA. Translation: AAH07567.1.
IPI	IPI00003406. IPI00295624.
PIR	JN0809. T14763.
RefSeq	NP_004386.2. NM_004395.3. NP_543157.1. NM_080881.2.
UniGene	Hs.130316.
3D structure databases
ProteinModelPortal	Q16643.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q16643. 11 interactions.
MINT	MINT-1149054.
STRING	9606.ENSP00000292385.
PTM databases
PhosphoSite	Q16643.
Polymorphism databases
DMDM	215274247.
2D gel databases
OGP	Q16643.
Proteomic databases
PaxDb	Q16643.
PRIDE	Q16643.
Protocols and materials databases
DNASU	1627.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000292385; ENSP00000292385; ENSG00000113758. ENST00000309007; ENSP00000308532; ENSG00000113758.
GeneID	1627.
KEGG	hsa:1627.
UCSC	uc003mgx.2. human. uc003mgy.2. human.
Organism-specific databases
CTD	1627.
GeneCards	GC05M176883.
H-InvDB	HIX0005464.
HGNC	HGNC:2695. DBN1.
HPA	CAB008367.
MIM	126660. gene.
neXtProt	NX_Q16643.
PharmGKB	PA27163.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG329456.
HOGENOM	HOG000015304.
HOVERGEN	HBG000823.
OrthoDB	EOG4R502Q.
Gene expression databases
ArrayExpress	Q16643.
Bgee	Q16643.
CleanEx	HS_DBN1.
Genevestigator	Q16643.
GermOnline	ENSG00000113758. Homo sapiens.
Family and domain databases
InterPro	IPR002108. Actin-bd_cofilin/tropomyosin. [Graphical view]
Pfam	PF00241. Cofilin_ADF. 1 hit. [Graphical view]
SMART	SM00102. ADF. 1 hit. [Graphical view]
PROSITE	PS51263. ADF_H. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	DBN1. human.
GenomeRNAi	1627.
NextBio	6674.
SOURCE	Search...

Entry information

Entry name

DREB_HUMAN

Accession

Primary (citable) accession number: Q16643
Secondary accession number(s): B2RBG0, Q9UFZ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 125 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.